Co-localization of the retinoblastoma protein and the Epstein-Barr virus-encoded nuclear antigen EBNA-5.

abstract：:By using affinity-purified antibodies to H10 and to H1AB the localization of these histones was studied by indirect immunofluorescence in the nuclei of proliferating (EAT and uninduced Friend cells) and of differentiating (induced Friend cells) cell populations. While with H1AB antibodies a bright fluorescence all ove...

journal_title：Experimental cell research

authors： Banchev T,Srebreva L,Zlatanova J,Tsanev R

更新日期：1988-07-01 00:00:00

abstract：:We have previously demonstrated that pretreatment of several cell lines with cis-unsaturated fatty acids, like oleic acid, blocks epidermal growth factor (EGF)-induced early ionic signals, and in particular the [Ca2+]i rise. In the present work we show that this blockade does not alter EGF-stimulated cellular prolifer...

journal_title：Experimental cell research

authors： Zugaza JL,Casabiell XA,Bokser L,Eiras A,Beiras A,Casanueva FF

更新日期：1995-07-01 00:00:00

abstract：:The addition of arachidonic acid (20:4), but not other fatty acids, including the structurally similar eicosapentaenoic acid (20:5), induced specific morphological changes in cultured endothelial cells derived from bovine aorta and pulmonary artery. Cells exhibited a time- and dose-dependent change from their normal, ...

journal_title：Experimental cell research

authors： Sandra A,Bar RS,Dolash S,Marshall SJ,Kaduce TL,Spector AA

更新日期：1985-06-01 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is fundamental for normal cell growth and organ development, but has also been implicated in various pathologies, notably tumors of epithelial origin. We have previously shown that the initial 13 amino acids (P13) within the intracellular juxtamembrane region (R645-R657) are...

journal_title：Experimental cell research

authors： Aifa S,Aydin J,Nordvall G,Lundström I,Svensson SP,Hermanson O

更新日期：2005-01-01 00:00:00

abstract：:In a previous study we have shown that triiodothyronine (T3) added to a serum-free medium supplemented with insulin, transferrin, and selenous acid (ITS) can stimulate Caco-2 cell differentiation. In this study we have focused on the effects of T3 on sucrase activity. The results obtained demonstrate that T3 (50 nM) d...

journal_title：Experimental cell research

authors： Jumarie C,Herring-Gillam FE,Beaulieu JF,Malo C

更新日期：1996-02-01 00:00:00

abstract：:The ocular surface shares many characteristics with mucosal surfaces. In both, healing is regulated by peptide growth factors, cytokines, and extracellular matrix proteins. However, these factors are not sufficient to ensure most rapid healing. Trefoil peptides are abundantly expressed epithelial cell products which e...

journal_title：Experimental cell research

authors： Göke MN,Cook JR,Kunert KS,Fini ME,Gipson IK,Podolsky DK

更新日期：2001-04-01 00:00:00

abstract：:hnRNP I, also referred to as polypyrimidine tract binding protein, is one of the proteins associated with nascent pre-mRNA in the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. As for all karyophilic proteins, the nuclear import of hnRNP proteins requires specific sequence determinants that in many instanc...

journal_title：Experimental cell research

authors： Romanelli MG,Weighardt F,Biamonti G,Riva S,Morandi C

更新日期：1997-08-25 00:00:00

abstract：:RNAi mediated loss of Drp1 function changes mitochondrial morphology in cultured HeLa and HUVEC cells by shifting the balance of mitochondrial fission and fusion towards unopposed fusion. Over time, inhibition of Drp1 expression results in the formation of a highly branched mitochondrial network along with "bulge"-lik...

journal_title：Experimental cell research

authors： Möpert K,Hajek P,Frank S,Chen C,Kaufmann J,Santel A

更新日期：2009-08-01 00:00:00

abstract：:The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and...

journal_title：Experimental cell research

authors： Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

更新日期：1990-02-01 00:00:00

abstract：:Elastin, a major constituent of the media, may play an important role in the attachment, migration, and phenotypic properties of vascular smooth muscle cells (SMC). We examined binding proteins and binding sites on elastin in rabbit arterial SMC in primary culture. Freshly isolated cells on alpha-elastin underwent phe...

journal_title：Experimental cell research

authors： Yamamoto K,Aoyagi M,Yamamoto M

更新日期：1995-05-01 00:00:00

abstract：:Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of v...

journal_title：Experimental cell research

authors： Yuan A,Chia CP

更新日期：2000-12-15 00:00:00

abstract：:The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for m...

journal_title：Experimental cell research

authors： Tu J,Zhang B,Fang G,Chang W,Zhao Y

更新日期：2020-05-15 00:00:00

abstract：:Autophagy is a major catabolic process allowing the renewal of intracellular organelles by which cells maintain their homeostasis. We have previously shown that autophagy is controlled by two transduction pathways mediated by a heterotrimeric Gi3 protein and phosphatidylinositol 3-kinase activities in the human colon ...

journal_title：Experimental cell research

authors： Bauvy C,Gane P,Arico S,Codogno P,Ogier-Denis E

更新日期：2001-08-15 00:00:00

abstract：:In the dystrophic pigmented Royal College of Surgeons (RCS) rat, the retinal pigment epithelium (RPE) has a diminished capacity to phagocytose shed photoreceptor outer segments (ROS). An alteration in phagocytic recognition or ligand-receptor interactions between the RPE and ROS's could contribute to this defect. To t...

journal_title：Experimental cell research

authors： Seyfried-Williams R,McLaughlin BJ,Cooper NG

更新日期：1984-10-01 00:00:00

abstract：:The long non-coding RNAs (lncRNAs) have gained much attention due to its essential roles in molecular regulation. As one of the classic lncRNAs, H19 is strongly expressed during embryogenesis and plays a crucial biological function during development. Mesenchymal stem cells (MSCs) are an ideal cell source for tissue e...

journal_title：Experimental cell research

authors： Liu Y,Li G,Zhang JF

更新日期：2017-11-15 00:00:00

abstract：:The epidermal growth factor (EGF) receptor is well known as a mediator of mitogenic signaling and its tyrosine kinase activity has been suggested as a viable target in cancer chemotherapy. To explore the consequences of abolishing the kinase activity of this receptor, we have utilized a potent and specific inhibitor o...

journal_title：Experimental cell research

authors： Nelson JM,Fry DW

更新日期：1997-06-15 00:00:00

abstract：:Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduce...

journal_title：Experimental cell research

authors： Deslex S,Negrel R,Ailhaud G

更新日期：1987-01-01 00:00:00

abstract：:Notch signaling involves the proteolytic cleavage of the transmembrane Notch receptor after binding to its transmembrane ligands. Jagged-1 also undergoes proteolytic cleavage by gamma-secretase and releases an intracellular fragment. In this study, we have demonstrated that the Jagged-1 intracellular domain (JICD) inh...

journal_title：Experimental cell research

authors： Kim MY,Jung J,Mo JS,Ann EJ,Ahn JS,Yoon JH,Park HS

更新日期：2011-10-15 00:00:00

abstract：:Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleo...

journal_title：Experimental cell research

authors： Schwab MS,Gossweiler U,Dreyer C

更新日期：1998-03-15 00:00:00

abstract：:We had earlier demonstrated that a 46-kDa glycoprotein is involved in the differentiation of rat skeletal myoblasts. We now show that the binding of this glycoprotein to collagen and gelatin is disrupted by Arg-Gly-Asp (RGD) containing peptide but not by Arg-Gly-Glu (RGE). The former peptide also selectively elutes th...

journal_title：Experimental cell research

authors： Nandan D,Cates GA,Ball EH,Sanwal BD

更新日期：1988-11-01 00:00:00

abstract：:Progressive pathophysiologic modifications of endothelial cells are associated with aging. In vitro, endothelial cell senescence is accompanied by the failure to proliferate as well as by perturbations in gene expression. Here we show that (i) senescence enhances monoblastoid U937 cell adhesion to the endothelial mono...

journal_title：Experimental cell research

authors： Maier JA,Statuto M,Ragnotti G

更新日期：1993-09-01 00:00:00

abstract：:The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...

journal_title：Experimental cell research

authors： Grøvdal LM,Johannessen LE,Rødland MS,Madshus IH,Stang E

更新日期：2008-04-01 00:00:00

abstract：:Over the last decade there has been growing evidence that Brain Expressed X-Linked 2 (BEX2) has a significant role in the process of carcinogenesis. Collectively, available studies suggest a pro-oncogenic function for this gene in multiple malignancies, including breast, colorectal and hepatocellular cancers in additi...

journal_title：Experimental cell research

authors： Naderi A

更新日期：2019-03-15 00:00:00

abstract：:Resistance of cancer cells to apoptosis is dependent on a balance of multiple genetic and epigenetic mechanisms, which up-regulate efficacy of the surviving growth factor-receptor signaling pathways and suppress death-receptor signaling pathways. The Insulin-like Growth Factor-1 Receptor (IGF-1R) signaling pathway is ...

journal_title：Experimental cell research

authors： Karasic TB,Hei TK,Ivanov VN

更新日期：2010-07-15 00:00:00

abstract：:In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...

journal_title：Experimental cell research

authors： Weitzel G,Pilatus U,Rensing L

更新日期：1987-05-01 00:00:00

abstract：:Mip130/LIN-9 is part of a large complex that includes homologs of the Drosophila dREAM (drosophila RB-like, E2F, and Myb) and C. elegans DRM complexes. This complex also includes proteins such as Mip40/LIN-37, Mip120/LIN-54, and LIN-52. In mammalian cells, Mip130/LIN-9 specifically associates with the p107/p130-E2F4 r...

journal_title：Experimental cell research

authors： Sandoval R,Pilkinton M,Colamonici OR

更新日期：2009-10-15 00:00:00

abstract：:Regulatory B cells (Breg cells) play critical roles in modulating immune responses during autoimmune diseases and infection. Here we explored the participation of two main Breg subsets, including IL-10+ Breg (B10) and IL-35+ Breg cells, in maintaining successful pregnancy. We first observed an elevated percentage of B...

journal_title：Experimental cell research

authors： Liu J,Chen X,Hao S,Zhao H,Pang L,Wang L,Ren H,Wang C,Mao H

更新日期：2019-10-15 00:00:00

abstract：:To study the biological function of Tetrahymena intermediate-type filament protein (a 49K protein), we examined the immunofluorescence localization of 49K protein within Tetrahymena cells. The results showed that the immunofluorescence was localized in the oral apparatus, mitochondria and mucocysts. Among them, the fl...

journal_title：Experimental cell research

authors： Numata O,Hirono M,Watanabe Y

更新日期：1983-10-01 00:00:00

abstract：:The role of glycans in the apical targeting of proteins in epithelial cells remains a debated question. We have expressed the mouse soluble dipeptidyl peptidase IV (DPP IV ectodomain) in kidney (MDCK) and in intestinal (Caco-2) epithelial cell lines, as a model to study the role of glycosylation in apical targeting. T...

journal_title：Experimental cell research

authors： Slimane TA,Lenoir C,Sapin C,Maurice M,Trugnan G

更新日期：2000-07-10 00:00:00

abstract：:Tendon is a critical unit of musculoskeletal system that connects muscle to bone to control bone movement. More population participate in physical activities, tendon injuries, such as acute tendon rupture and tendinopathy due to overuse, are common causing unbearable pain and disability. However, the process of tendon...

journal_title：Experimental cell research

authors： Lu YF,Chan KM,Li G,Zhang JF

更新日期：2016-02-15 00:00:00