Abstract：
:In a previous study we have shown that triiodothyronine (T3) added to a serum-free medium supplemented with insulin, transferrin, and selenous acid (ITS) can stimulate Caco-2 cell differentiation. In this study we have focused on the effects of T3 on sucrase activity. The results obtained demonstrate that T3 (50 nM) does not change Caco-2 cell proliferation but enhances sucrase activity from 50 to 80%. Similar increases were observed whether or not insulin was present in the culture medium, showing that there was no synergistic effect between T3 and insulin on sucrase activity. Moreover, T3 acts specifically during the differentiation period since addition of T3 to the defined TS medium before confluency is reached does not stimulate sucrase activity. Sucrase kinetic parameters were evaluated for the first time in Caco-2 cells under various culture conditions. The presence of a single enzyme was verified, with a Km of about 7 mM and a Vmax around 20 nmol of substrate hydrolyzed min-1 mg-1 of protein. Our results showed that T3 did not change the enzyme's affinity for sucrose but doubled the Vmax. Moreover, immunoblotting using anti-sucrase-isomaltase (SI) antibodies revealed an approximately twofold increase in the relative amount of SI immunoreactive protein in T3-stimulated cells compared to untreated cells. Results obtained by both Northern hybridization and RT-PCR amplification showed a significant increase in SI mRNA contents. These results suggest that T3 acts primarily on sucrase expression at the mRNA level.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Jumarie C,Herring-Gillam FE,Beaulieu JF,Malo Cdoi
10.1006/excr.1996.0041subject
Has Abstractpub_date
1996-02-01 00:00:00pages
319-25issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(96)90041-5journal_volume
222pub_type
杂志文章abstract：:Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.5096
更新日期：2001-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.3464
更新日期：1997-02-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1381
更新日期：1995-12-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2019-10-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90330-8
更新日期：1988-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90640-2
更新日期：1984-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1998.4278
更新日期：1999-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90503-8
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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doi：10.1006/excr.1998.4176
更新日期：1998-09-15 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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更新日期：1992-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.12.016
更新日期：2013-02-15 00:00:00
abstract：:Multipotent mesenchymal stromal cells (MSCs) support wound healing processes. These cells express toll-like receptors (TLRs). TLRs perform important key functions when the immune system is confronted with danger signals. TLR ligation by lipopolysaccharides (LPS) activates MSCs and induces intracellular signaling casca...
journal_title：Experimental cell research
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更新日期：2018-09-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.07.018
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2005.11.025
更新日期：2006-04-01 00:00:00
abstract：:The effect of hydrocortisone (HC) on PDGF beta-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF beta-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF beta-recepto...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(05)80074-6
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