Triiodothyronine stimulates the expression of sucrase-isomaltase in Caco-2 cells cultured in serum-free medium.

Abstract:

:In a previous study we have shown that triiodothyronine (T3) added to a serum-free medium supplemented with insulin, transferrin, and selenous acid (ITS) can stimulate Caco-2 cell differentiation. In this study we have focused on the effects of T3 on sucrase activity. The results obtained demonstrate that T3 (50 nM) does not change Caco-2 cell proliferation but enhances sucrase activity from 50 to 80%. Similar increases were observed whether or not insulin was present in the culture medium, showing that there was no synergistic effect between T3 and insulin on sucrase activity. Moreover, T3 acts specifically during the differentiation period since addition of T3 to the defined TS medium before confluency is reached does not stimulate sucrase activity. Sucrase kinetic parameters were evaluated for the first time in Caco-2 cells under various culture conditions. The presence of a single enzyme was verified, with a Km of about 7 mM and a Vmax around 20 nmol of substrate hydrolyzed min-1 mg-1 of protein. Our results showed that T3 did not change the enzyme's affinity for sucrose but doubled the Vmax. Moreover, immunoblotting using anti-sucrase-isomaltase (SI) antibodies revealed an approximately twofold increase in the relative amount of SI immunoreactive protein in T3-stimulated cells compared to untreated cells. Results obtained by both Northern hybridization and RT-PCR amplification showed a significant increase in SI mRNA contents. These results suggest that T3 acts primarily on sucrase expression at the mRNA level.

journal_name

Exp Cell Res

authors

Jumarie C,Herring-Gillam FE,Beaulieu JF,Malo C

doi

10.1006/excr.1996.0041

subject

Has Abstract

pub_date

1996-02-01 00:00:00

pages

319-25

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(96)90041-5

journal_volume

222

pub_type

杂志文章
  • Transformation and characterization of mutant human fibroblasts defective in peroxisome assembly.

    abstract::Human skin fibroblasts deficient in peroxisome biogenesis were transformed by transfecting SV40 ori- DNA with the use of an electroporator, and the biochemical, immunocytochemical, and cytogenetic properties of the transformants were analyzed. Cells (1 x 10(6)) from a patient with Zellweger syndrome and one with neona...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(92)90278-g

    authors: Okamoto H,Suzuki Y,Shimozawa N,Yajima S,Masuno M,Orii T

    更新日期:1992-08-01 00:00:00

  • Stage and tissue-specific expression of a collagen gene during Drosophila melanogaster development.

    abstract::Based on data from developmental RNA profiles and in situ hybridization, we report a direct examination of the expression of one collagen gene (Dcg1) during drosophila melanogaster life cycle. These studies show, for the first time, that the expression of a collagen gene is both differential and tissue-specific during...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90071-6

    authors: Le Parco Y,Knibiehler B,Cecchini JP,Mirre C

    更新日期:1986-04-01 00:00:00

  • Effect of shear stress on cytosolic Ca2+ of calf pulmonary artery endothelial cells.

    abstract::The purpose of the present study was to determine if hemodynamic shear stress increases free cytosolic Ca2+ concentration ([Ca2+]i) of cultured pulmonary artery endothelial cells exposed to steady laminar fluid flow in a parallel plate chamber. Average [Ca2+]i was estimated by measuring cell-associated fura-2 fluoresc...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(92)90145-x

    authors: Schilling WP,Mo M,Eskin SG

    更新日期:1992-01-01 00:00:00

  • The recycling of a secretory granule membrane protein.

    abstract::We have used N-hydroxysuccinimido-d-biotin as a reagent for labeling proteins exposed at the surface of cultured bovine adrenal chromaffin cells during Ba2+-stimulated secretion. A specific secretory granule membrane constituent, dopamine-beta-hydroxylase (DBH), has been investigated using immunoprecipitation followed...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(89)90249-8

    authors: Hunter A,Phillips JH

    更新日期:1989-06-01 00:00:00

  • In chicken leukemia cells globin genes are fully transcribed but their rnas are retained in the perinucleolar area.

    abstract::Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2001.5332

    authors: Iarovaia O,Razin SV,Linares-Cruz G,Sjakste N,Scherrer K

    更新日期:2001-11-01 00:00:00

  • A syndecan-4 binding peptide derived from laminin 5 uses a novel PKCε pathway to induce cross-linked actin network (CLAN) formation in human trabecular meshwork (HTM) cells.

    abstract::In this study, we examined the role(s) of syndecan-4 in regulating the formation of an actin geodesic dome structure called a cross-linked actin network (CLAN) in which syndecan-4 has previously been localized. CLANs have been described in several different cell types, but they have been most widely studied in human t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2014.07.035

    authors: Filla MS,Clark R,Peters DM

    更新日期:2014-10-01 00:00:00

  • Measurement of cell-monolayer adhesion in cells transfected with N-CAM cDNAs.

    abstract::To measure the adhesion of cells expressing the neural cell adhesion molecule N-CAM, mouse Lmtk- fibroblast cells were transfected by a calcium phosphate precipitation technique with eucaryotic expression vectors encoding N-CAM polypeptides. We obtained cell lines expressing the 140-kDa transmembrane isoform of N-CAM ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1041

    authors: Woo MK,Murray BA

    更新日期:1993-02-01 00:00:00

  • Myristoylation is required for the intracellular localization and endocytic function of ARF6.

    abstract::ADP-ribosylation factors (ARFs) are modified by a myristate group that is covalently linked to the second glycine residue at the amino terminus. With the recent evidence that ARF6 localizes to the cell periphery and plays a regulatory role in endocytic traffic, we have investigated the role of myristoylation on the me...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1362

    authors: D'Souza-Schorey C,Stahl PD

    更新日期:1995-11-01 00:00:00

  • Epigenetic silencing in embryogenesis.

    abstract::Transcription profiling of early embryos emphasizes that differential gene expression is a fundamental control mechanism of development. Precise regulatory mechanisms operate on a background of epigenetic changes in chromatin composition, modification, and architecture that are integral for the development of a plurip...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2005.06.023

    authors: Meehan RR,Dunican DS,Ruzov A,Pennings S

    更新日期:2005-10-01 00:00:00

  • The structural analysis of adhesions mediated by Ep-CAM.

    abstract::The epithelial cell adhesion molecule Ep-CAM is capable of mediating Ca2+-independent homotypic cell-cell adhesion when introduced into cells lacking their own means of cell-cell interactions. We used (confocal) immunofluorescent and (immuno-) electron microscopy to investigate the structural organization of Ep-CAM-me...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4263

    authors: Balzar M,Prins FA,Bakker HA,Fleuren GJ,Warnaar SO,Litvinov SV

    更新日期:1999-01-10 00:00:00

  • Purification and characterization of new mating pheromones of the ciliate Euplotes raikovi.

    abstract::Cell union in mating pairs in the ciliate Euplotes raikovi is controlled by a system of multiple mating types which are inherited with alleles codominant at the genetic locus mat and expressed via diffusible mating pheromones. The mating pheromones Er-2, Er-3, and Er-11 were purified from cells homozygous for the mat-...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(87)90399-5

    authors: Raffioni S,Miceli C,Concetti A,Barra D,Luporini P

    更新日期:1987-10-01 00:00:00

  • Tissue-specific posttranslational modification of the small heat shock protein HSP27 in Drosophila.

    abstract::Drosophila sHSPs (small heat shock proteins) are expressed in the absence of stress in specific regions of the central nervous system and in gonads of young adults flies. In these two organs, the sHSPs show a cell-specific and developmental stage-specific pattern of expression suggesting distinct regulation and functi...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1996.0052

    authors: Marin R,Landry J,Tanguay RM

    更新日期:1996-02-25 00:00:00

  • Cell cycle specificity and reversibility of the inhibitory effect of epidermal G1-chalone on DNA synthesis in partially synchronized RTE-2 keratinocytes in vitro.

    abstract::The specific action of a pig skin fraction enriched in epidermal G1-chalone, a tissue-specific inhibitor of epidermal DNA synthesis, was investigated by means of flow cytofluorometry. The results indicate that G1-chalone inhibits progression of partially synchronized rat tongue epithelial cells (line RTE-2) through th...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(89)90235-8

    authors: Tiegel A,Richter KH,Marks F

    更新日期:1989-06-01 00:00:00

  • Genetic and epigenetic changes in clonal descendants of irradiated human fibroblasts.

    abstract::To study delayed genetic and epigenetic radiation effects, which may trigger radiation-induced carcinogenesis, we have established single-cell clones from irradiated and non-irradiated primary human fibroblasts. Stable clones were endowed with the same karyotype in all analyzed metaphases after 20 population doublings...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2018.06.034

    authors: Flunkert J,Maierhofer A,Dittrich M,Müller T,Horvath S,Nanda I,Haaf T

    更新日期:2018-09-15 00:00:00

  • Lymphoblastoid cell variant in contact-mediated cell spreading.

    abstract::We have isolated a clone of human lymphoblastoid cells that is capable of undergoing the phenomenon of contact-mediated cell spreading in vitro. We have detected this behavior when using both transmission electron microscopy (TEM), and differential interference contrast microscopy. Upon cell-cell contact, cells become...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(83)90015-0

    authors: Sorrentino JA,Millis AJ,Whipple AP,Colquhoun WR

    更新日期:1983-05-01 00:00:00

  • Compartmentalization of Fas and Fas ligand may prevent auto- or paracrine apoptosis in epithelial cells.

    abstract::Oligomerization of Fas receptor by its ligand, FasL, activates a signaling cascade that leads to apoptosis of Fas bearing cells. Interestingly, many epithelia coexpress Fas and FasL, yet FasL does not trigger Fas present on the same or neighboring cells to induce spontaneous apoptosis. Here, we show that Fas and FasL ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/s0014-4827(02)00056-3

    authors: Tan KH,Hunziker W

    更新日期:2003-04-01 00:00:00

  • Specific activation of a STAT family member in Xiphophorus melanoma cells.

    abstract::Melanoma formation in the teleost fish Xiphophorus is caused by the uncontrolled activity of the genetically defined tumor locus Tu. The critical component of this locus is the Xmrk oncogene encoding a subclass I receptor tyrosine kinase. Overexpression and constitutive activation of the Xmrk receptor triggers a set o...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4470

    authors: Baudler M,Schartl M,Altschmied J

    更新日期:1999-06-15 00:00:00

  • Macrophages enhance tumor-derived autophagosomes (DRibbles)-induced B cells activation by TLR4/MyD88 and CD40/CD40L.

    abstract::Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2014.10.015

    authors: Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

    更新日期:2015-02-15 00:00:00

  • Cellular fibronectin expression is down-regulated at the mRNA level in differentiating human intestinal epithelial cells.

    abstract::The expression of human cellular fibronectin (HFN) was analyzed in relation to the differentiation process of the human enterocyte-like cell line Caco-2. A single approximately 290-kDa form of HFN, corresponding to a 10.0-kb mRNA species, was observed in Caco-2 cells. The expression levels of both protein and mRNA wer...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1004

    authors: Vachon PH,Simoneau A,Herring-Gillam FE,Beaulieu JF

    更新日期:1995-01-01 00:00:00

  • Actin organization during the cell cycle in meristematic plant cells. Actin is present in the cytokinetic phragmoplast.

    abstract::The distribution and organisation of F-actin during the cell cycle of meristematic root-tip cells of Allium was investigated using a rhodamine-labelled phalloidin to stain F-actin in isolated cell preparations. Such preparations could, in addition, be stained for tubulin by immunofluorescence, enabling a comparison be...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(85)90277-0

    authors: Clayton L,Lloyd CW

    更新日期:1985-01-01 00:00:00

  • A newly established porcine aortic endothelial cell line: characterization and application to the study of human-to-swine graft rejection.

    abstract::The establishment of cell lines allows reproductible in vitro studies that would be far more difficult to perform using primary cells that rapidly undergo phenotypical alterations in culture. The purpose of this work was to establish an endothelial cell line appropriate for in vitro study of endothelial cell activatio...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3831

    authors: Malassagne B,Taboit F,Conti F,Batteux F,Atia N,Chéreau C,Conjeaud H,Théron MC,Attal J,Braet F,Houdebine LM,Calmus Y,Houssin D,Weill B

    更新日期:1998-01-10 00:00:00

  • Serum and serotonin induce retraction of calf aortic smooth muscle (CASM) cells in vitro: inhibition by ketanserin, a 5-HT2 receptor antagonist.

    abstract::Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(90)90216-w

    authors: De Wever B,Rooman RP,De Brabander M

    更新日期:1990-01-01 00:00:00

  • pH in the endosome. Measurements during pinocytosis and receptor-mediated endocytosis.

    abstract::By fluorescence spectroscopy, the average pH within endocytic compartments was determined during endocytosis of fluorescein conjugates by macrophages and hepatocytes. In mouse macrophages and hepatocytes fluorescein conjugates taken up either in the fluid phase or by binding to cell surface receptors were rapidly tran...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90699-2

    authors: Geisow MJ,Evans WH

    更新日期:1984-01-01 00:00:00

  • High expression levels of the "erythroid/brain" type glucose transporter (GLUT1) in the basal cells of human eye conjunctiva and oral mucosa reconstituted in culture.

    abstract::The expression of the "erythroid/brain" type glucose transporter (GLUT1) seems to be a feature of "barrier" tissues, at least in humans. Recently, we reported that GLUT1 is highly expressed in the basal layers of either "authentic" human epidermis or human epidermis reconstituted in culture and that its expression see...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(91)90522-v

    authors: Gherzi R,Melioli G,De Luca M,D'Agostino A,Guastella M,Traverso CE,D'Anna F,Franzi AT,Cancedda R

    更新日期:1991-07-01 00:00:00

  • An antibody against 100- to 116-kDa polypeptides in coated vesicles inhibits triskelion binding.

    abstract::There is considerable evidence that the 100- to 116-kDa polypeptides in calf brain coated vesicles are involved in the assembly of clathrin triskelions to form coated vesicles. We have raised polyclonal antibodies against these polypeptides. By Western blot analysis, these antibodies bind to a distinct subset of the s...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(88)90320-5

    authors: Mosley ST,Branton D

    更新日期:1988-02-01 00:00:00

  • Decreased tumorigenicity of c-Myc-transformed fibroblasts expressing active USF2.

    abstract::USF is a small family of basic helix-loop-helix leucine zipper (bHLH-zip) transcription factors with DNA binding specificities similar to that of the c-Myc oncoprotein. Evidence for a role of USF in growth control includes inhibition of c-Myc-dependent cellular transformation in vitro and loss of USF transcriptional a...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2004.08.013

    authors: Choe C,Chen N,Sawadogo M

    更新日期:2005-01-01 00:00:00

  • Overexpression of soluble ADAM33 promotes a hypercontractile phenotype of the airway smooth muscle cell in rat.

    abstract::A disintegrin and metalloproteinase 33 (ADAM33) has been identified as a susceptibility gene for asthma, but details of the causality are not fully understood. We hypothesize that soluble ADAM33 (sADAM33) overexpression can alter the mechanical behaviors of airway smooth muscle cells (ASMCs) via regulation of the cell...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2016.10.004

    authors: Duan Y,Long J,Chen J,Jiang X,Zhu J,Jin Y,Lin F,Zhong J,Xu R,Mao L,Deng L

    更新日期:2016-11-15 00:00:00

  • Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1.

    abstract::Leptomycin B (LMB) is a Streptomyces metabolite that inhibits nuclear export of the human immunodeficiency virus type 1 regulatory protein Rev at low nanomolar concentrations. Recently, LMB was shown to inhibit the function of CRM1, a receptor for the nuclear export signal (NES). Here we show evidence that LMB binds d...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4136

    authors: Kudo N,Wolff B,Sekimoto T,Schreiner EP,Yoneda Y,Yanagida M,Horinouchi S,Yoshida M

    更新日期:1998-08-01 00:00:00

  • Conventional protein kinase C mediates phorbol-dibutyrate-induced cytoskeletal remodeling in a7r5 smooth muscle cells.

    abstract::Phorbol dibutyrate (PDBu) induced the formation of podosome-like structures together with partial disassembly of actin stress fibers in A7r5 smooth muscle cells. These podosomes contained alpha-actinin, F-actin, and vinculin and exhibit a tubular, column-like structure arising perpendicularly from the bottom of PDBu-t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2002.5592

    authors: Hai CM,Hahne P,Harrington EO,Gimona M

    更新日期:2002-10-15 00:00:00

  • Down-regulation of PKHD1 induces cell apoptosis through PI3K and NF-κB pathways.

    abstract::Mutations in PKHD1 (polycystic kidney and hepatic disease gene 1) gene cause the autosomal recessive polycystic kidney disease (ARPKD). Fibrocystin/polyductin (FPC), encoded by PKHD1, is a membrane-associated receptor-like protein. Although it is widely accepted that cystogenesis is mostly due to aberrant cell prolife...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2011.01.025

    authors: Sun L,Wang S,Hu C,Zhang X

    更新日期:2011-04-15 00:00:00