The cytoplasmic pH, ATP content and total protein synthesis rate during heat-shock protein inducing treatments in yeast.

abstract：:LC3, a mammalian homologue of yeast Atg8, is assumed to play an important part in bulk sequestration and degradation of cytoplasm (macroautophagy), and is widely used as an indicator of this process. To critically examine its role, we followed the autophagic flux of LC3 in rat hepatocytes during conditions of maximal ...

journal_title：Experimental cell research

authors： Szalai P,Hagen LK,Sætre F,Luhr M,Sponheim M,Øverbye A,Mills IG,Seglen PO,Engedal N

更新日期：2015-04-10 00:00:00

abstract：:The existence of two different pathways for cell death has been postulated. In addition to the passive and traumatic process leading to necrosis, an active program characterized by organelle integrity and called apoptosis has been described. A positive correlation between the apoptotic cell death process and oxidative...

journal_title：Experimental cell research

authors： Cossarizza A,Franceschi C,Monti D,Salvioli S,Bellesia E,Rivabene R,Biondo L,Rainaldi G,Tinari A,Malorni W

更新日期：1995-09-01 00:00:00

abstract：:Compact mouse morulae were decompacted in calcium-free medium and allowed to recompact in standard embryo culture medium. When the recompaction medium contained trifluoperazine (TFP)(0.05 mM), an inhibitor of the calcium-dependent protein calmodulin, the embryos failed to recompact. This effect could not be overcome b...

journal_title：Experimental cell research

authors： Bilozur M,Powers RD

更新日期：1982-11-01 00:00:00

abstract：:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...

journal_title：Experimental cell research

authors： Nakamura H,Morita T,Sato C

更新日期：1986-08-01 00:00:00

abstract：:Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT,...

journal_title：Experimental cell research

authors： Premzl A,Zavasnik-Bergant V,Turk V,Kos J

更新日期：2003-02-15 00:00:00

abstract：:Coiled bodies are conserved subnuclear organelles that contain splicing snRNPs, a subset of nucleolar antigens, and the autoantigen p80 coilin. Most nuclei contain one to five nucleoplasmic coiled bodies, often with one or more located at the nucleolar periphery. Here we show that exposure of mammalian cells to low le...

journal_title：Experimental cell research

authors： Lyon CE,Bohmann K,Sleeman J,Lamond AI

更新日期：1997-01-10 00:00:00

abstract：:Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...

journal_title：Experimental cell research

authors： Paiement J

更新日期：1986-10-01 00:00:00

abstract：:Fluid-phase endocytosis was significantly faster in three lines of human lysosomal storage disease fibroblasts (two I cells and one Sandhoff's) than in three normals. Re-exocytosis of fluid showed similar kinetics in all lines when release was expressed as a percentage of the radioactivity associated with the cells at...

journal_title：Experimental cell research

authors： Dean RT

更新日期：1984-04-01 00:00:00

abstract：:When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increa...

journal_title：Experimental cell research

authors： Rønning OW,Pettersen EO

更新日期：1984-11-01 00:00:00

abstract：:A great deal of evidence suggests that long non-coding RNAs (lncRNAs) function in the tumorigenesis of retinoblastoma (RB). However, the roles of lncRNA ILF3-AS1 in RB are still unclear. In the present study, our work revealed that the lncRNA ILF3-AS1 was increased in both RB tissues and cell lines. Repression of ILF3...

journal_title：Experimental cell research

authors： Han S,Song L,Chen Y,Hou M,Wei X,Fan D

更新日期：2020-08-15 00:00:00

abstract：:The phosphorylation of glucose-regulated protein, GRP78, is thought to be involved in the regulation of the binding function of GRP78 to immunoglobulin heavy chains. The phosphorylation of GRP78 proceeded faster in transformed cells than in normal cells, whereas the levels of GRP78 synthesis and accumulation were simi...

journal_title：Experimental cell research

authors： Satoh M,Nakai A,Sokawa Y,Hirayoshi K,Nagata K

更新日期：1993-03-01 00:00:00

abstract：:Galectin-3, a beta-galactoside-binding protein, has been shown to be involved in multiple biological processes through interaction with its complementary glycoconjugates. Here we provide the first evidence of galectin-3 as a mitogen. Incubation of quiescent cultures of normal human lung fibroblast IMR-90 cells with re...

journal_title：Experimental cell research

authors： Inohara H,Akahani S,Raz A

更新日期：1998-12-15 00:00:00

abstract：:The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...

journal_title：Experimental cell research

authors： Stuurman N,Van Driel R,De Jong L,Meijne AM,Van Renswoude J

更新日期：1989-02-01 00:00:00

abstract：:Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I...

journal_title：Experimental cell research

authors： Freise C,Bobb V,Querfeld U

更新日期：2017-09-15 00:00:00

abstract：:Celiac disease (CD) is a multisystemic autoimmune inflammation of the intestinal tract induced by wheat gluten and related cereals in HLA-DQ2/8 positive individuals. An essential role in the pathogenesis of CD is played by a fraction of the peptic-tryptic digest of gluten, Frazer's Fraction (FF). Here, we investigate ...

journal_title：Experimental cell research

authors： Reinke Y,Zimmer KP,Naim HY

更新日期：2009-11-15 00:00:00

abstract：:The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...

journal_title：Experimental cell research

authors： Nicolson GL

更新日期：1993-02-01 00:00:00

abstract：:We have previously shown that following psoralen photoactivation (PUVA treatment) human dermal fibroblasts undergo long-term growth arrest as well as morphological and functional changes reminiscent of cellular senescence [ 1 ]. In the absence of molecular data on what constitutes normal senescence, it has been diffic...

journal_title：Experimental cell research

authors： Ma W,Wlaschek M,Brenneisen P,Schneider LA,Hommel C,Hellweg C,Sauer H,Wartenberg M,Herrmann G,Meewes C,Boukamp P,Scharffetter-Kochanek K

更新日期：2002-04-01 00:00:00

abstract：:Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleo...

journal_title：Experimental cell research

authors： Schwab MS,Gossweiler U,Dreyer C

更新日期：1998-03-15 00:00:00

abstract：:This study deals with the effects of hyper- and hypo-osmotic media on the ultrastructure of four different types of cells and tissues: rat pheochromocytoma cells of line PC12, mouse Ehrlich ascites tumor cells, rat kidney cortex and intestine. Application of hyper-osmotic conditions induces in the nuclear compartment ...

journal_title：Experimental cell research

authors： Delpire E,Duchêne C,Goessens G,Gilles R

更新日期：1985-09-01 00:00:00

abstract：:We have compared the rate of synthesis, half-life, and steady-state content of the oncoprotein p53 in logarithmically growing cultures of (a) primary embryo, (b) immortalized but untransformed, and (c) spontaneously transformed mouse fibroblasts. Steady-state p53 content derived from metabolic labeling and immunopreci...

journal_title：Experimental cell research

authors： Williams NW,Wynford-Thomas D

更新日期：1989-10-01 00:00:00

abstract：:The process of osteoblast differentiation and matrix mineralization requires a rise in alkaline phosphatase enzymatic activity resulting in the generation of free phosphate. The ability of inorganic phosphate to regulate gene transcription and cellular function represents a potentially novel extracellular signaling me...

journal_title：Experimental cell research

authors： Beck GR Jr,Moran E,Knecht N

更新日期：2003-08-15 00:00:00

abstract：:Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...

journal_title：Experimental cell research

authors： Law AY,Lai KP,Ip CK,Wong AS,Wagner GF,Wong CK

更新日期：2008-05-01 00:00:00

abstract：:The glial cell line-derived neurotrophic factor (GDNF) is involved in the development and maintenance of neural tissues. Mutations in components of its signaling pathway lead to severe migration deficits of neuronal crest stem cells, tumor formation, or ablation of the urinary system. In animal models of Parkinson's d...

journal_title：Experimental cell research

authors： Pahnke J,Mix E,Knoblich R,Müller J,Zschiesche M,Schubert B,Koczan D,Bauer P,Böttcher T,Thiesen HJ,Lazarov L,Wree A,Rolfs A

更新日期：2004-07-15 00:00:00

abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...

journal_title：Experimental cell research

authors： Kinnaird JH,Maitland K,Walker GA,Wheatley I,Thompson FJ,Devaney E

更新日期：2004-08-15 00:00:00

abstract：:We present the first evidence that secretory organelle docking at the cell membrane can be reversed in vivo. In nondischarge (nd) mutants of Paramecium tetraurelia all trichocysts can be detached from the cell surface within 2-3 h by different means, including cytochalasin B (but not D), high cell density, or Ca2+ ion...

journal_title：Experimental cell research

authors： Pape R,Plattner H

更新日期：1990-12-01 00:00:00

abstract：:Two genes, KIN1 and KIN2, were isolated in Saccharomyces cerevisiae by their homology to the protein kinase family of viral oncogenes. In previous studies, we identified the yeast KIN1 gene product (pp145KIN1) and KIN2 gene product (pp145KIN2) as 145 kDa phosphoproteins with serine/threonine protein kinase activity. W...

journal_title：Experimental cell research

authors： Tibbetts M,Donovan M,Roe S,Stiltner AM,Hammond CI

更新日期：1994-07-01 00:00:00

abstract：:Binding of integrins to the extracellular matrix (ECM) activates various signal transduction pathways and regulates gene expression in many cell types. Integrin-dependent cytoplasmic protein/protein interactions are necessary for activation of those signal transduction cascades. In our studies we investigated a possib...

journal_title：Experimental cell research

authors： Danker K,Gabriel B,Heidrich C,Reutter W

更新日期：1998-03-15 00:00:00

abstract：:Upregulated ERK1/2 activity is often correlated with AKT activation during prostate cancer (PCa) progression, yet their functional relation needs elucidation. Using androgen-deprived LNCaP cells, in which ERK1/2 activation occurs in strong correlation with AKT activation, we found that AKT-mediated B-Raf regulation is...

journal_title：Experimental cell research

authors： Hong SK,Jeong JH,Chan AM,Park JI

更新日期：2013-07-15 00:00:00

abstract：:Wnt1 and -5a have been shown to modulate the proliferation and differentiation of midbrain dopaminergic (DA) neurons. However, it is not known whether other Wnts or which Frizzled (Fz) receptors are expressed in the developing midbrain. We found that 13 out of 19 Wnts, all 10 Fzs, and several intracellular Wnt signali...

journal_title：Experimental cell research

authors： Rawal N,Castelo-Branco G,Sousa KM,Kele J,Kobayashi K,Okano H,Arenas E

更新日期：2006-05-15 00:00:00

abstract：:Bcl-2 family proteins regulate apoptosis at the level of mitochondria. To examine the mechanism of Bcl-2 function, we investigated the effects of the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP) on two hematopoietic cell lines and Bcl-2 overexpressing transfectants. CCCP directly interferes with mitoc...

journal_title：Experimental cell research

authors： de Graaf AO,van den Heuvel LP,Dijkman HB,de Abreu RA,Birkenkamp KU,de Witte T,van der Reijden BA,Smeitink JA,Jansen JH

更新日期：2004-10-01 00:00:00