Abstract：
:Two genes, KIN1 and KIN2, were isolated in Saccharomyces cerevisiae by their homology to the protein kinase family of viral oncogenes. In previous studies, we identified the yeast KIN1 gene product (pp145KIN1) and KIN2 gene product (pp145KIN2) as 145 kDa phosphoproteins with serine/threonine protein kinase activity. We have used two approaches to determine the location of these protein kinases in the yeast cell: biochemical fractionation and purification of plasma membrane and cell wall material. Biochemical fractionation techniques revealed that 70-90% of both KIN1 and KIN2 proteins localized to a particulate fraction. This particulate fraction contained three pools of KIN1 and KIN2 proteins. One pool could be released with either sodium chloride or high pH, another released with nonionic detergent, and a third was resistant to either treatment. Thus, both protein-protein and protein-membrane interactions are responsible for the association to the particulate fraction. The presence of these proteins in purified plasma membranes confirms the biochemical fractionation data demonstrating a protein-membrane interaction. Interestingly, a significant fraction of KIN1 and KIN2 proteins are present in purified cell wall preparations. Since protease protection experiments indicate that these proteins are confined to the cytoplasmic face of the plasma membrane and do not protrude into the periplasmic space, any association with the cell wall must be mediated through a protein complex.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Tibbetts M,Donovan M,Roe S,Stiltner AM,Hammond CIdoi
10.1006/excr.1994.1177subject
Has Abstractpub_date
1994-07-01 00:00:00pages
93-9issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(84)71177-3journal_volume
213pub_type
杂志文章abstract：:The dynamics of lamin disassembly and reassembly during sea urchin male pronuclear development in vitro was investigated. Using five anti-lamin antibodies, we monitored by immunofluorescence and immunoblotting the changes in lamins during sperm chromatin decondensation, nuclear envelope (NE) formation, and male pronuc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1280
更新日期：1995-08-01 00:00:00
abstract：:Formation of the neural tube is the morphological hallmark for development of the embryonic central nervous system (CNS). Therefore, neural tube development is a crucial step in the neurulation process. Slit/Robo signaling was initially identified as a chemo-repellent that regulated axon growth cone elongation, but it...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.02.011
更新日期：2013-05-01 00:00:00
abstract：:3-phosphoinositide-dependent kinase-1 (PDK1) phosphorylates and activates several kinases in the cAMP-dependent, cGMP-dependent and protein kinase C (AGC) family. Many putative PDK1 substrates have been identified, but have not been analyzed following transient and specific inhibition of PDK1 activity. Here, we demons...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.04.006
更新日期：2008-07-01 00:00:00
abstract：:Olfactory ensheathing cells (OECs) are a type of glial cells with morphological plasticity in the olfactory system. Cultured OECs display the process-bearing and flattened shape. Our previous studies have shown that the frontal application of Slit-2 gradient induced the collapse of leading front, and reversed the soma...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.05.024
更新日期：2012-09-10 00:00:00
abstract：:A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90173-x
更新日期：1987-08-01 00:00:00
abstract：:The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1993.1022
更新日期：1993-02-01 00:00:00
abstract：:Earlier we reported the undetectable level of endothelin (ET)-1 mRNA in aorta from young donors, in contrast to the detectable levels in older donors (Lab. Invest. 67, 210-217, 1992). We also found that the synthesis of ET-1 peptide is elevated in cultured endothelial cells from the aorta of over-50-year-old donors. I...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1051
更新日期：1994-03-01 00:00:00
abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.08.010
更新日期：2011-01-01 00:00:00
abstract：:Colon cancer shows characteristics of metastasis, which is associated with angiogenesis. Increasing evidence highlights long non-coding RNAs (lncRNAs) as important participants in angiogenesis of cancers, including colon cancer. Hence, this study investigated the role of HNF1A-AS1 in angiogenesis of colon cancer. RT-q...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112025
更新日期：2020-08-15 00:00:00
abstract：:The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90709-2
更新日期：1984-01-01 00:00:00
abstract：:T51B rat liver epithelial cells were stimulated with extracellular ATP. Changes in cytoplasmic free Ca2+ concentration [( Ca2+]i) were measured by fura-2 both in a large population of cells on coverslips in a cuvette and in single cells in a microscopic system. Extracellular ATP evoked a prompt increase in [Ca2+]i in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90198-5
更新日期：1989-03-01 00:00:00
abstract：:The stimulatory heterotrimeric Gs protein alpha subunit (Gsα) is a ubiquitous guanine nucleotide-binding protein that regulates the intracellular cAMP signaling pathway and consequently participates in a wide range of biological events. In the reproductive system, despite Gsα being associated with oocyte meiotic arres...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.07.023
更新日期：2018-09-15 00:00:00
abstract：:A respiratory epithelial cell line M3E3/C3, derived from fetal Syrian hamster lung, grew very slowly in RPMI 1640 medium with serum concentrations as low as 1.5%. Under these conditions the simultaneous action of epidermal growth factor (EGF) and vitamin A (retinol) was apparently a combination of growth enhancement a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90578-1
更新日期：1986-03-01 00:00:00
abstract：:The role of the F-actin cytoskeleton in cell volume regulation was studied in Ehrlich ascites tumor cells, using a quantitative rhodamine-phalloidin assay, confocal laser scanning microscopy, and electronic cell sizing. A hypotonic challenge (160 mOsm) was associated with a decrease in cellular F-actin content at 1 an...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4615
更新日期：1999-10-10 00:00:00
abstract：:In the fungus Achlya, sexual development in the male strain E87 is mediated by the steroid hormone antheridiol. Treatment of vegetatively growing cells of E87 with antheridiol resulted in changes in the [35S]methionine labeling of several secreted proteins. The most heavily labeled group of proteins observed in the se...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90555-0
更新日期：1986-03-01 00:00:00
abstract：:Skeletal muscles cope with a large range of activities, from being able to support the body weight during long periods of upright standing to perform explosive movements in response to an unexpected threat. This requires systems for energy metabolism that can provide energy during long periods of moderately increased ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2010.05.019
更新日期：2010-11-01 00:00:00
abstract：:Tubular epithelial-to-mesenchymal transition (EMT) plays a crucial role in the progression of renal tubular interstitial fibrosis (TIF), which subsequently leads to chronic kidney disease (CKD) and eventually, end-stage renal disease (ESRD). We propose that augmenter of liver regeneration (ALR), a member of the newly ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.07.001
更新日期：2014-10-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) is a pleiotropic cytokine promoting proliferation, migration and survival in several cell types. HGF and its cognate receptor c-Met are expressed in cardiac cells during early cardiogenesis, but data concerning its role in cardiac differentiation of embryonic stem cells (ESCs) and the un...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.12.009
更新日期：2007-03-10 00:00:00
abstract：:Time-lapse microcinematography was used to trace the migration and subsequent fate of daughter pairs from single myogenic stem cells. The generation times of siblings which divide are closely correlated (r, 0.73) compared with randomly selected cells (r, 0.059), suggesting the more or less equal partition of stem cell...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90204-1
更新日期：1986-11-01 00:00:00
abstract：:Laminin is a major component of the basal lamina upon which all epithelial cells reside in vivo. The synthesis of basal lamina components and their subsequent assembly into a morphologically distinct basal lamina is a differentiated function of epithelial cells in vivo. Ultrastructural studies in our laboratory show t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90036-8
更新日期：1992-11-01 00:00:00
abstract：:Murine embryonal carcinoma F9 cells can be induced to differentiate by 2-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC). The differentiated phenotype is similar to that of retinoic acid (RA)-treated F9 cells. In contrast to F9 cells the differentiated cells secrete plasminog...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90276-9
更新日期：1985-01-01 00:00:00
abstract：:We previously demonstrated that the Tg737 gene plays a critical role in the carcinogenesis of hepatocellular carcinoma (HCC). However, few systematic investigations have focused on the biological function of Tg737 in the invasion and migration of liver cancer stem cells (LCSCs) and on its clinical significance. In thi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.06.021
更新日期：2017-09-15 00:00:00
abstract：:Human lymphocytes were either exposed to X-irradiation (25 to 200 rads) or treated with H2O2 (9.1 to 291 microM) at 4 degrees C and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Both agents induced a significant increase in DNA migration, beg...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90265-0
更新日期：1988-03-01 00:00:00
abstract：:Microsomal triglyceride transfer protein (MTP) is a heterodimeric complex consisting of a unique large 97-kDa protein and the multifunctional 58-kDa protein disulfide isomerase (PDI). It plays an essential role in the assembly of lipoproteins by shuttling lipids between phospholipid membranes. Based on cell fractionat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.05.038
更新日期：2004-10-15 00:00:00
abstract：:Accumulating evidence shows that the inhibition of thromboxane synthase (TXS) induced apoptosis in cancer cells. TXS inhibitor 1-Benzylimidzole (1-BI) can trigger apoptosis in lung cancer cells but the mechanism is not fully defined. In this study, lung cancer cells were treated with 1-BI. In this study, the level of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.07.003
更新日期：2010-12-10 00:00:00
abstract：OBJECTIVE:To investigate whether transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor (bFGF)-incorporating degradable stent implantation (TMDRSI) can promote myocardial regeneration after acute myocardial infarction (AMI). METHODS:A model of AMI was generated by ligating ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.11.009
更新日期：2012-02-15 00:00:00
abstract：:To gain insight into the role of the endothelial cell during the pathophysiology of the angiogenic response, investigators have isolated micro- and macrovascular endothelial cells from a wide range of both animal and human vessels, including the umbilical vein. Human umbilical vein endothelial cells (HUVECs) isolated ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0168
更新日期：1996-05-25 00:00:00
abstract：:Vero cells were infected with the ts-045 strain of vesicular stomatitis virus, and the cells were incubated at 39 degrees C to accumulate the mutant G glycoprotein in the ER as a misfolded aggregate. Cycloheximide was added to the culture medium 3.5 h after infection to prevent further protein synthesis, and the tempe...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0281
更新日期：1996-09-15 00:00:00
abstract：:In many systems, events participating in cell division are controlled by intracellular pH (pHi). In Xenopus eggs, fertilization is accompanied by an increase in pHi which occurs concomitantly with an increase in protein synthesis and a reinitiation of DNA synthesis, leading the embryo to cell division. In this paper, ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90313-3
更新日期：1989-12-01 00:00:00
abstract：:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90583-5
更新日期：1986-08-01 00:00:00
