Abstract：
:The dynamics of lamin disassembly and reassembly during sea urchin male pronuclear development in vitro was investigated. Using five anti-lamin antibodies, we monitored by immunofluorescence and immunoblotting the changes in lamins during sperm chromatin decondensation, nuclear envelope (NE) formation, and male pronuclear swelling in fertilized sea urchin egg cytoplasmic extracts. We report the existence of five proteins in sperm nuclei and swollen male pronuclei (p49, p54, p65, p72, p84) which react with the antibodies. The sperm antigens resist membrane permeabilization with lysolecithin and 0.1% Triton X (TX)-100, but are removed from the lateral aspects of the nuclei by 1% TX-100. All five are completely removed from nuclei within 10 min of incubation in egg extracts. Initial chromatin decondensation and NE formation occur without reassembly of the putative lamins, but all lamins or lamin epitope-containing peptides assemble coordinately during pronuclear swelling promoted by adenosine 5'-triphosphate. Of the five pronuclear antigens, p49 and p54 appear to originate exclusively from the sperm. p65, p72, and p84 are also present in the egg cytoplasm and may be contributed to pronuclei by either source. Assembly of putative lamins and nuclear swelling, but not chromatin decondensation and nuclear envelope formation, are prevented in lamin-depleted cytoplasmic extracts. Our results indicate that p49 and p54 are not necessary for nuclear swelling, cytoplasmic p65 is required for swelling, and p72 and p84 are by themselves not sufficient but may be involved with p65 in nuclear swelling and full pronuclear development in vitro.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Collas P,Pinto-Correia C,Poccia DLdoi
10.1006/excr.1995.1280subject
Has Abstractpub_date
1995-08-01 00:00:00pages
687-98issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(85)71280-3journal_volume
219pub_type
杂志文章abstract：:Among the cellular events that are associated with the process of endochondral ossification is an incremental increase in chondrocyte basal intracellular free Ca(2+) concentration ([Ca(2+)](i)) from 50 to 100 nM. To determine if this rise in [Ca(2+)](i) functionally participates in the maturational process of growth p...
journal_title：Experimental cell research
pub_type： 杂志文章
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abstract：:Accumulating evidence shows that the inhibition of thromboxane synthase (TXS) induced apoptosis in cancer cells. TXS inhibitor 1-Benzylimidzole (1-BI) can trigger apoptosis in lung cancer cells but the mechanism is not fully defined. In this study, lung cancer cells were treated with 1-BI. In this study, the level of ...
journal_title：Experimental cell research
pub_type： 杂志文章
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abstract：:Using a device named the cell force monitor, the contractile force developed by fibroblasts has been studied by measuring the macroscopic contraction of porous collagen-glycosaminoglycan (GAG) matrices over the first 24 h following cell attachment. In this paper, the effect of a variation in the stiffness that resists...
journal_title：Experimental cell research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental cell research
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更新日期：1989-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1985-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1995-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1998-11-25 00:00:00
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pub_type： 杂志文章
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更新日期：1992-12-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2004-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2010-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1986-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2008-07-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2010-11-15 00:00:00
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pub_type： 杂志文章
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更新日期：2003-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1987-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2006-09-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3831
更新日期：1998-01-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1356
更新日期：1995-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2012-09-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2014-01-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1332
更新日期：1995-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2003-07-15 00:00:00
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journal_title：Experimental cell research
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更新日期：2017-03-15 00:00:00
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journal_title：Experimental cell research
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更新日期：2019-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2001-04-01 00:00:00
abstract：:Human amnion epithelial cells attach and flatten slowly (approximately 65 min) onto plastic in the presence of serum but much more rapidly (20-30 min) onto subcellular matrix (SCM) deposited by the same cells. This matrix contains both fibronectin and laminin, but neither molecule on its own can reproduce its adhesive...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90611-6
更新日期：1984-08-01 00:00:00
