Abstract:
:We have used biotinylated rDNA probes to localize by in situ hybridization the extrachromosomal genes for ribosomal RNA in the slime mold Physarum polycephalum. We established conditions that allow for highly specific hybridization at the ultrastructural level and determined that the 60-kb palindromic rDNA molecules are confined to the nucleolus in interphase. Our study definitively locates these extrachromosomal genes in mitosis in the form of thin DNA fibers contained within nucleolar remnants. We further show that these rDNA minichromosomes do not condense and that they segregate as entities independent of the condensed chromosomal DNA. In telophase, these minichromosomes migrate from the poles toward the equatorial region of the nucleus in a direction opposite that of the chromosomes. Our results illustrate the discontinuous nature of the nucleolar organizing region in Physarum.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Puvion-Dutilleul F,Pierron Gdoi
10.1016/0014-4827(92)90009-wkeywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
354-64issue
2eissn
0014-4827issn
1090-2422journal_volume
203pub_type
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journal_title:Experimental cell research
pub_type: 杂志文章
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