Abstract:
:The establishment of cell lines allows reproductible in vitro studies that would be far more difficult to perform using primary cells that rapidly undergo phenotypical alterations in culture. The purpose of this work was to establish an endothelial cell line appropriate for in vitro study of endothelial cell activation during xenograft rejection. Porcine aortic endothelial cells were transfected with the early region of SV40 and selected on the basis of morphological, phenotypical, and functional features. By light and electron microscopy, the porcine aortic endothelial cell line (PAEC11) and primary cells were similar except that PAEC11 was slightly smaller. PAEC11 displayed endothelial cell characteristics since it endocytosed acetylated low density lipoproteins, produced von Willebrand factor, and expressed E-selectin. Human natural antibodies bound to the same xenoantigens on PAEC11 and primary cells. That binding was followed by human complement activation and cell lysis. In addition, PAEC11 was found appropriate for genetic engineering since it could be transfected with a plasmid encoding a foreign gene. Therefore, this cell line should be a useful model for in vitro study of endothelial cell function in general and human-to-swine xenograft rejection in particular.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Malassagne B,Taboit F,Conti F,Batteux F,Atia N,Chéreau C,Conjeaud H,Théron MC,Attal J,Braet F,Houdebine LM,Calmus Y,Houssin D,Weill Bdoi
10.1006/excr.1997.3831subject
Has Abstractpub_date
1998-01-10 00:00:00pages
90-100issue
1eissn
0014-4827issn
1090-2422pii
S0014482797938313journal_volume
238pub_type
杂志文章abstract::Ran, a nuclear GTPase, and a number of interacting proteins, including regulators RanGEF1 and RanGAP1, are involved in nucleocytoplasmic transport. We have identified a new temperature-sensitive mutation in budding yeast YRB1 gene, which encodes Ran-binding protein-1 (RanBP1). In contrast to other yrb1 alleles, the ne...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4174
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abstract::Previous hybrid studies involving fusion of normal with immortal human cells indicated that the phenotype of cellular senescence is dominant and that immortality results from recessive changes in normal growth regulatory genes. We have further assigned 28 different immortal human cell lines to at least four complement...
journal_title:Experimental cell research
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abstract::In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis...
journal_title:Experimental cell research
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abstract::Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nu...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1016/0014-4827(85)90277-0
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abstract::The DNA topoisomerase I inhibitor camptothecin (CAM) is selectively cytotoxic to S-phase cells of HL-60, and some other myelogenous leukemic lines. The early effects of cell exposure to 0.05-0.2 micrograms/ml CAM are seen after 2 h; at that time a progressive degradation of DNA in the chromatin of S-phase cells is ini...
journal_title:Experimental cell research
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doi:10.1016/0014-4827(91)90534-2
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90149-5
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1306
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.05.037
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90318-2
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0223
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3799
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abstract::To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three ke...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.01.011
更新日期:2004-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1263
更新日期:1993-10-01 00:00:00
abstract::K252a, a protein kinase inhibitor, acts as a neurotrophic factor in several neuronal cells. In this study we show that K252a enhanced the differentiation of C2C12 myoblasts as well as tyrosine phosphorylation of several focal adhesion-associated proteins including p130(Cas), focal adhesion kinase, and paxillin. The ty...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4648
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abstract::There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4256
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abstract::Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...
journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.05.024
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3680
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90100-4
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.10.027
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abstract::The turbidity of axonemes during active sliding of microtubules was analysed using the stopped-flow-light-scattering method with high time resolution. Flagella of sea-urchin spermatozoa were demembranated and used after a brief treatment with trypsin. The turbidity of the suspension of flagellar axonemes during ATP-in...
journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4411
更新日期:1999-04-10 00:00:00
abstract::The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3876
更新日期:1998-02-25 00:00:00