Tetracyclines cause cell stress-dependent ATF4 activation and mTOR inhibition.

abstract：:To gain insight into the role of the endothelial cell during the pathophysiology of the angiogenic response, investigators have isolated micro- and macrovascular endothelial cells from a wide range of both animal and human vessels, including the umbilical vein. Human umbilical vein endothelial cells (HUVECs) isolated ...

journal_title：Experimental cell research

authors： Hughes SE

更新日期：1996-05-25 00:00:00

abstract：:Nuclear pore complexes (NPCs) provide the only sites for macromolecular transport between nucleus and cytoplasm. The nucleoporin p62, a component of higher eukaryotic NPCs, is located at the central gated channel and involved in nuclear trafficking of various cargos. p62 is organized into an N-terminal segment that co...

journal_title：Experimental cell research

authors： Stochaj U,Bański P,Kodiha M,Matusiewicz N

更新日期：2006-08-01 00:00:00

abstract：:HALF1 (human activator of liver function 1) is a closed DNA duplex implicated in reversion of rat dedifferentiated C2 hepatoma cells to a well-differentiated state. A copy of HALF1 is found in high-molecular-weight DNA in the human genome. The genomic equivalent of HALF1 and its flanking sequences [gH(5'-3') fragment]...

journal_title：Experimental cell research

authors： Boccaccio C,Meunier-Rotival M,Deschatrette J

更新日期：1994-07-01 00:00:00

abstract：:Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in China with a poor prognosis. Most ESCC patients were diagnosed at advanced stages, losing the opportunity for surgical excision. Hence, it remains a pressing work to identify biomarkers for early detection, prognosis prediction and target...

journal_title：Experimental cell research

authors： Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

更新日期：2018-06-01 00:00:00

abstract：:Cells with and without hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity were used to examine the transfer of purine metabolites through the medium and via cell contacts. HGPRT- Chinese hamster and human fibroblasts were able to incorporate 3H-labeled purine metabolite(s) from medium in which mouse HGPRT...

journal_title：Experimental cell research

authors： Bols NC,Mosser DD,Boliska SA

更新日期：1987-11-01 00:00:00

abstract：:The bioflavonoid quercetin is a dietary anticancer chemical that is capable of inducing apoptosis in tumor cells. Although the activity of quercetin is believed to be due to its antioxidative properties, it has recently been suggested that quercetin also has prooxidant activities, which might effect cytotoxicity direc...

journal_title：Experimental cell research

authors： Lee JC,Kim J,Park JK,Chung GH,Jang YS

更新日期：2003-12-10 00:00:00

abstract：:Poly (ADP-ribose) polymerase-1 (PARP1) is a DNA damage sensor that gets activated in proportion to the damage, helping cells to determine whether to repair the damage or initiate cell death processes. We have previously shown PARP1's significance in the developmental processes of Dictyostelium discoideum in addition t...

journal_title：Experimental cell research

authors： Mir H,Rajawat J,Vohra I,Vaishnav J,Kadam A,Begum R

更新日期：2020-12-15 00:00:00

abstract：:Polyamines affect nucleosome oligomerization and DNA conformation in vitro, yet little information exists regarding the influence of naturally synthesized polyamines on mammalian chromatin. Capitalizing on the relative inefficiency of a moderate ionic strength extraction buffer to dissociate histones, we obtained evid...

journal_title：Experimental cell research

authors： Hobbs CA,Paul BA,Gilmour SK

更新日期：2003-11-01 00:00:00

abstract：:In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...

journal_title：Experimental cell research

authors： Johnson-Pais TL,Leach RJ

更新日期：1996-07-10 00:00:00

abstract：:By using affinity-purified antibodies to H10 and to H1AB the localization of these histones was studied by indirect immunofluorescence in the nuclei of proliferating (EAT and uninduced Friend cells) and of differentiating (induced Friend cells) cell populations. While with H1AB antibodies a bright fluorescence all ove...

journal_title：Experimental cell research

authors： Banchev T,Srebreva L,Zlatanova J,Tsanev R

更新日期：1988-07-01 00:00:00

abstract：:Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...

journal_title：Experimental cell research

authors： Vaughan AT,Gordon DJ

更新日期：1992-10-01 00:00:00

abstract：:We have previously established the malignant T lymphoma CS-21 cell line from a spontaneous lymphoma in a BALB/c mouse. CS-21 lymphoma cells grew continuously when they were cocultured with stromal CA-12 cells prepared from lymph nodes. CS-21 lymphoma cells, however, could not proliferate by themselves, and they underw...

journal_title：Experimental cell research

authors： Kataoka S,Naito M,Fujita N,Ishii H,Ishii S,Yamori T,Nakajima M,Tsuruo T

更新日期：1993-08-01 00:00:00

abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...

journal_title：Experimental cell research

authors： Li X,Lu H,Gu Y,Zhang X,Zhang G,Shi T,Chen W

更新日期：2020-01-01 00:00:00

abstract：BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

journal_title：Experimental cell research

authors： Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

更新日期：2019-10-01 00:00:00

abstract：:In this study an amphotropic retrovirus has been used to efficiently transduce normal human (NF) and scleroderma (systemic sclerosis; SSc) dermal fibroblasts (SScF) with a sequence encoding a temperature-sensitive mutant of the SV40 large T antigen (tsA58-U19). From the primary outgrowths of skin explants, cultures we...

journal_title：Experimental cell research

authors： Xu S,Vancheeswaran R,Bou-Gharios G,O'Hare MJ,Olsen I,Abraham D,Black C

更新日期：1995-10-01 00:00:00

abstract：:Previously, whole-mount electron microscopy of nuclei extruded together with residual cytoplasm from the rhizoids of several algal species of the order Dasycladales has revealed the occurrence of an intra- and perinuclear network of 10-nm filaments morphologically indistinguishable from that of mammalian vimentin inte...

journal_title：Experimental cell research

authors： Berger S,Wittke W,Traub P

更新日期：1998-05-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...

journal_title：Experimental cell research

authors： Angus SP,Fribourg AF,Markey MP,Williams SL,Horn HF,DeGregori J,Kowalik TF,Fukasawa K,Knudsen ES

更新日期：2002-06-10 00:00:00

abstract：:During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for...

journal_title：Experimental cell research

authors： Bryantsev AL,Chechenova MB,Shelden EA

更新日期：2007-01-01 00:00:00

abstract：:In a previous study, we found that a specific inhibitor of cellular arginine-specific mono(ADP-ribosyl) transferase, meta-iodobenzylguanidine (MIBG), reversibly inhibited both proliferation and differentiation of cultured embryonic chick primary muscle myoblasts. In addition, we observed that arginine-specific ADP-rib...

journal_title：Experimental cell research

authors： Huang HY,Zhou H,Huiatt TW,Graves DJ

更新日期：1996-07-10 00:00:00

abstract：:We report here the first direct observation of chemotaxis to EGF by rat mammary carcinoma cells. When exposed to a gradient of EGF diffusing from a micropipette, MTLn3 cells displayed typical ameboid chemotaxis, extending a lamellipod-like protrusion and moving toward the pipette. Using a homogeneous upshift in EGF to...

journal_title：Experimental cell research

authors： Bailly M,Yan L,Whitesides GM,Condeelis JS,Segall JE

更新日期：1998-06-15 00:00:00

abstract：:Most coronary smooth muscle cells(CoSMCs) are differentiated from epicardial progenitor cells(EPCs), but the specific mechanism is not fully investigated. Previous studies have shown that autophagy plays an important role for smooth muscle cells(SMCs) differentiation, yet whether autophagy is involved in the different...

journal_title：Experimental cell research

authors： Liu Y,Liu B,Li Y,Li Y,Du J,Deng S,Jing X,She Q

更新日期：2019-02-15 00:00:00

abstract：:Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I...

journal_title：Experimental cell research

authors： Freise C,Bobb V,Querfeld U

更新日期：2017-09-15 00:00:00

abstract：:The phosphorylation of glucose-regulated protein, GRP78, is thought to be involved in the regulation of the binding function of GRP78 to immunoglobulin heavy chains. The phosphorylation of GRP78 proceeded faster in transformed cells than in normal cells, whereas the levels of GRP78 synthesis and accumulation were simi...

journal_title：Experimental cell research

authors： Satoh M,Nakai A,Sokawa Y,Hirayoshi K,Nagata K

更新日期：1993-03-01 00:00:00

abstract：:Studies were carried out to test the idea that transforming growth factor beta (TGFbeta) stimulated fibroblast contraction of collagen matrices by different mechanisms depending on mechanical loading on the cells and matrix. Under mechanically unloaded conditions (floating matrices), TGFbeta stimulated contraction dir...

journal_title：Experimental cell research

authors： Grinnell F,Ho CH

更新日期：2002-02-15 00:00:00

abstract：:The structure and the polypeptide composition of the nuclear shell isolated from interphase HeLa cells have been investigated and compared to those of the intranuclear material. The isolated nuclear shell contains chromatin superstructures (28-32 nm thick fibres) made of tightly packed nucleosomes that resist low ioni...

journal_title：Experimental cell research

authors： Bouvier D,Hubert J,Seve AP,Bouteille M

更新日期：1985-02-01 00:00:00

abstract：:Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...

journal_title：Experimental cell research

authors： Paillasson S,Van De Corput M,Dirks RW,Tanke HJ,Robert-Nicoud M,Ronot X

更新日期：1997-02-25 00:00:00

abstract：:Despite an increasing interest in the role of the p53 tumour suppressor protein in embryonic stem cells, not much is known about its regulation in this cell type. We show that the relatively high amount of p53 protein correlates with a higher amount of p53 RNA in ES cells compared to differentiated cells. Moreover, p5...

journal_title：Experimental cell research

authors： Solozobova V,Blattner C

更新日期：2010-09-10 00:00:00

abstract：:Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...

journal_title：Experimental cell research

authors： Saggioro D,D'agostino DM,Chieco-Bianchi L

更新日期：1999-03-15 00:00:00

abstract：:Raver2 was originally identified as a member of the hnRNP family through database searches revealing three N-terminal RNA recognition motifs (RRMs) bearing highest sequence identity in the RNP sequences to the related hnRNP Raver1. Outside the RRM region, both Raver proteins are quite divergent in sequence except for ...

journal_title：Experimental cell research

authors： Henneberg B,Swiniarski S,Sabine Becke,Illenberger S

更新日期：2010-04-01 00:00:00

abstract：:In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...

journal_title：Experimental cell research

authors： Weitzel G,Pilatus U,Rensing L

更新日期：1987-05-01 00:00:00