Abstract：
:HALF1 (human activator of liver function 1) is a closed DNA duplex implicated in reversion of rat dedifferentiated C2 hepatoma cells to a well-differentiated state. A copy of HALF1 is found in high-molecular-weight DNA in the human genome. The genomic equivalent of HALF1 and its flanking sequences [gH(5'-3') fragment] have previously been cloned and sequenced. To analyze the ability of the gH(5'-3') fragment to induce reversion process of C2 cells, two series of transfections were performed: (1) cotransfection of gH(5'-3') and plasmid pSVneo1 and (2) transfection of gH(5'-3') inserted into pSVneo1. The frequency of reversion was enhanced in transfected cells from the first experiment whereas no revertants were obtained from transfected cells in the second one. DNA analysis of the revertant clones revealed that reversion is associated with the transient presence of nonintegrated gH(5'-3') molecules. C2 cells were also transfected with gH(5'-3') cloned in pSV2dhfr. In the products of this transfection, the genesis of revertants correlated with amplification process of the gH(5'-3') sequence. We conclude that the presence of integrated copies of gH(5'-3'), even in high copy number, is not sufficient to induce the reversion process. We propose that extrachromosomal forms of gH(5'-3'), either given to cells or formed during amplification cycles, are involved in the reversion process of C2 cells.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Boccaccio C,Meunier-Rotival M,Deschatrette Jdoi
10.1006/excr.1994.1180subject
Has Abstractpub_date
1994-07-01 00:00:00pages
113-20issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(84)71180-3journal_volume
213pub_type
杂志文章abstract：:The mechanisms of replicative senescence by telomere shortening are not fully understood. The Indian muntjac has the fewest chromosomes of all mammals, greatly simplifying the analysis of each telomere over time. In this study, telomere shortening was observed throughout the life span of cultured normal muntjac cells ...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2002-11-15 00:00:00
abstract：:Neurons require adhesive scaffolds for their growth and differentiation. Laminins are a major cell adhesive component of basement membranes and have various biological activities in the peripheral and central nervous systems. Here, we evaluated the biological activities of 5 peptides derived from laminin-111 as a scaf...
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abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2006.02.029
更新日期：2006-07-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1207
更新日期：1994-08-01 00:00:00
abstract：:Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...
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更新日期：2005-05-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2016.07.025
更新日期：2016-08-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：1999-11-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2005-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.07.001
更新日期：2014-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90176-5
更新日期：1987-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90262-x
更新日期：1987-07-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90140-5
更新日期：1985-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4586
更新日期：1999-09-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.07.010
更新日期：2008-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.12.002
更新日期：2013-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.08.007
更新日期：2017-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4331
更新日期：1999-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.04.020
更新日期：2013-08-15 00:00:00
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journal_title：Experimental cell research
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更新日期：2000-02-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.04.014
更新日期：2010-07-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90389-c
更新日期：1991-08-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2011.09.007
更新日期：2011-12-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.05.009
更新日期：2013-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2004-07-01 00:00:00
abstract：:High throughput RNA sequencing techniques have revealed that a large fraction of the genome is transcribed into long non-coding RNAs (lncRNAs). Unlike canonical protein-coding genes, lncRNAs do not contain long open reading frames (ORFs) and tend to be poorly conserved across species. However, many of them contain sma...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2020-06-01 00:00:00
abstract：:Uptake and metabolism of thymidine and adenosine have been studied in embryos of the sea urchin Strongylocentrotus purpuratus. Uptake of these nucleosides is found to be mutually competitive, with the Km for uptake of thymidine similar to its Ki for inhibition of adenosine uptake and vice versa. The metabolic studies ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90411-3
更新日期：1987-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.01.025
更新日期：2018-03-01 00:00:00
abstract：:Tendon is a critical unit of musculoskeletal system that connects muscle to bone to control bone movement. More population participate in physical activities, tendon injuries, such as acute tendon rupture and tendinopathy due to overuse, are common causing unbearable pain and disability. However, the process of tendon...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2015.12.014
更新日期：2016-02-15 00:00:00
