Abstract：
:In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed by fusion of the human osteosarcoma TE-85 with the mouse fibrosarcoma La-t- demonstrated a 10-fold reduction of ALPL steady-state mRNA and enzyme activity, a phenomenon termed extinction. Hybrid subclones which reexpressed ALPL contained reduced numbers of fibroblast chromosomes compared to earlier passages. This suggests that a trans-acting negative regulatory factor expressed from the fibroblast genome regulates ALPL expression. Two factors known to influence ALPL expression are 1,25-dihydroxyvitamin D3 (1,25D3) and transforming growth factor-beta1 (TGFbeta1). 1,25D3 is involved in mobilizing bone calcium stores and TGFbeta1 plays a critical role in bone remodeling. The extinguished hybrids were exposed to 1,25D3, TGFbeta1, and a combination of these factors. For two hybrids, the combination induced reexpression of ALPL activity to levels comparable to the TE-85 parent, indicating a competition between the factors and the extinguisher(s). Neither factor alone could induce ALPL reexpression to the levels observed with the combination. In only one hybrid, the combination of factors synergistically increased ALPL expression. These data help define the cis sequence element(s) in the ALPL promoter which are involved in the negative regulation of this gene.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Johnson-Pais TL,Leach RJsubject
Has Abstractpub_date
1996-07-10 00:00:00pages
67-74issue
1eissn
0014-4827issn
1090-2422journal_volume
226pub_type
杂志文章abstract：:Melanoma development is a multi-step process arising from a series of genetic and epigenetic events. Although the sequential stages involved in progression from melanocytes to malignant melanoma are clearly defined, our current understanding of the mechanisms leading to melanoma onset is still incomplete. Growing evid...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.12.023
更新日期：2009-03-10 00:00:00
abstract：:This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90099-v
更新日期：1990-04-01 00:00:00
abstract：:The small GTPases Rho, Rac, and Cdc42 regulate the actin cytoskeleton in all eukaryotic cells. In this study we have evaluated the effect of cholesterol oxides (7-ketocholesterol and 25-hydroxycholesterol) on cell migration, cell adhesion, and cytoskeletal organisation of lens epithelial cells (LEC). Effects of choles...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.08.002
更新日期：2003-12-10 00:00:00
abstract：:Malignant melanoma (MM) remains the leading cause of skin cancer related death, which has very poor prognosis because of locoregional recurrence and distant metastasis. DEPDC1B (DEP domain-containing protein 1B), has been proved to be associated with some types of malignant tumors. However, the role of DEPDC1B in MM i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.03.021
更新日期：2019-06-01 00:00:00
abstract：:The adenosine analog 5,6 dichloro-1-beta-ribofuranosyl benzimidazole (DRB) inhibits specifically mRNA production in eukaryotic cells, with concomitant alterations in pre-rRNA processing and nucleolar ultrastructure. We have focused our analysis on the effects of drug treatment on rRNA transcription through observation...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90322-1
更新日期：1983-06-01 00:00:00
abstract：:We report here the first direct observation of chemotaxis to EGF by rat mammary carcinoma cells. When exposed to a gradient of EGF diffusing from a micropipette, MTLn3 cells displayed typical ameboid chemotaxis, extending a lamellipod-like protrusion and moving toward the pipette. Using a homogeneous upshift in EGF to...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4031
更新日期：1998-06-15 00:00:00
abstract：:Impaired elastin fiber assembly is observed in the fetal ductus arteriosus (DA), associated with a reduced concentration of elastin binding protein (EBP), a 67-kDa galactolectin. It is also seen in cultured aortic (Ao) smooth muscle cells (SMC) following the release of the EBP by glycosaminoglycans rich in N-acetylgal...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90008-v
更新日期：1992-12-01 00:00:00
abstract：:This study investigates how epithelial cells moving together function to coordinate their collective movement to repair a wound. Using a lens ex vivo mock cataract surgery model we show that region-specific reorganization of cell-cell junctions, cytoskeletal networks and myosin function along apical and basal domains ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.12.021
更新日期：2014-03-10 00:00:00
abstract：:The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.09.030
更新日期：2017-11-15 00:00:00
abstract：:The first angiogenesis inhibitors for cancer have now been approved by the F.D.A. in the U.S. and in 28 other countries, including China. The majority of these are monotherapies that block VEGF. However, mutant tumor cells may over time produce redundant angiogenic factors. Therefore, for long-term use in cancer, comb...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2005.11.015
更新日期：2006-03-10 00:00:00
abstract：:Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.12.011
更新日期：2016-07-01 00:00:00
abstract：:Sperm cells from control donors of proven fertility and men from barren couples were studied by conventional procedures, i.e., light microscopy as well as flow cytometry. Light microscopy analysis of semen included the measurement of spermatozoa concentration, morphology, and motility. All the men from barren couples ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4064
更新日期：1998-06-15 00:00:00
abstract：:SIRT5 has a wide range of functions in different cellular processes such as glycolysis, TCA cycle and antioxidant defense, which mediates lysine desuccinylation, deglutarylation and demalonylation. Recent evidences have implicated that SIRT5 is a potential suppressor of gastric cancer (GC). However, the underlying mec...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.06.028
更新日期：2019-09-15 00:00:00
abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.10.015
更新日期：2015-02-15 00:00:00
abstract：:Abscission marks the completion of cell division and its failure is associated with delayed cytokinesis and even tetraploidization. Aberrant abscission and consequential ploidy changes can underlie various diseases including cancer. Midbody, a transient structure formed in the intercellular bridge during telophase, co...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.08.019
更新日期：2011-01-15 00:00:00
abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.05.008
更新日期：2008-08-01 00:00:00
abstract：:As a widely used anti-bacterial agent and a metabolic inhibitor as well as AMP-activated protein kinase (AMPK) activator, berberine (BBR) has been shown to cross the blood-brain barrier. Its efficacy has been investigated in various disease models of the central nervous system. Neurite outgrowth is critical for nervou...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.04.006
更新日期：2015-06-10 00:00:00
abstract：:Cross-linking of CD44 in vitro promotes chemokinesis and actin-based dendrite formation in T and B cells. However, the mechanisms by which the adhesion molecule CD44 induces cytoskeleton activation in lymphocytes are still poorly understood. In this study, we have investigated whether myosin isoforms are involved in C...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.002
更新日期：2006-10-15 00:00:00
abstract：:The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleola...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1110
更新日期：1993-04-01 00:00:00
abstract：:We have studied the ability of plasmids encoding a normal human myc protein to stimulate growth of primary rat embryo fibroblasts. We measured growth stimulation by the number of G418-resistant colonies obtained after co-transfection with plasmid pSV2neo and by the percentage of these colonies that grew in long-term c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90482-9
更新日期：1986-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90611-6
更新日期：1984-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4253
更新日期：1998-12-15 00:00:00
abstract：:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0123
更新日期：1996-04-10 00:00:00
abstract：:There are a number of low-abundance small nuclear RNAs (snRNAs) in eukaryotic cells. Many of them have been assigned functions in the biogenesis of cellular RNAs, such as splicing and 3' end processing. Here, we present the sequence of Xenopus U12 snRNA and compare the secondary structures of the low-abundance U11 and...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0372
更新日期：1996-12-15 00:00:00
abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.08.010
更新日期：2011-01-01 00:00:00
abstract：:Tunicamycin, an inhibitor of one of the earliest steps in the synthesis of N-linked oligosaccharides, prevents bud formation and growth in Saccharomyces cerevisiae cells that are either growing exponentially or recovering from different cell cycle arrests at start. Analysis of tunicamycin-treated cells by flow microfl...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90176-5
更新日期：1987-08-01 00:00:00
abstract：:Culture and UV (254 nm) irradiation conditions that are suggested as appropriate for a study of the effect of UV on the limited in vitro lifespan of a normal human diploid fibroblast (HDF) strain are first described. An inoculation density at each subcultivation of 1.8 x 10(4) viable cells/cm-2 permits the decline in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(78)90368-3
更新日期：1978-05-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is one of the most common cancers diagnosed worldwide. However, the mechanism underlying HCC pathogenesis remains unknown. In the present study, TRIM24 was found increased in human HCC clinical samples and positively correlated with HCC tumor grade. Furthermore, TRIM24 knockdown inhibits...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.04.006
更新日期：2018-06-15 00:00:00
abstract：:An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To identify pathways downstream of PKP3 loss that are required for increased tumour formation, a gene expression analysis was performed, which demonstrated that the expression of lipocalin2 (LCN2) was elevated upon PKP3 loss and...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.05.026
更新日期：2018-08-15 00:00:00
abstract：:The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90072-4
更新日期：1989-02-01 00:00:00
