Ultrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes.

abstract：:Despite over 30 years of work, the fundamental structure of eukaryotic chromatin remains controversial. Here, we review the roots of this controversy in disparities between results derived from studies of chromatin in nuclei, chromatin isolated from nuclei, and chromatin reconstituted from defined components. Thanks t...

journal_title：Experimental cell research

authors： Grigoryev SA,Woodcock CL

更新日期：2012-07-15 00:00:00

abstract：:Pitx2 is a bicoid-related homeobox transcription factor implicated in regulating left-right patterning and organogenesis. However, only a limited number of Pitx2 downstream target genes have been identified and characterized. Here we demonstrate that Pitx2 is a transcriptional repressor of DEP domain containing 1B (DE...

journal_title：Experimental cell research

authors： Wu D,Zhu X,Jimenez-Cowell K,Mold AJ,Sollecito CC,Lombana N,Jiao M,Wei Q

更新日期：2015-04-10 00:00:00

abstract：:The cellular targets for the Kirsten murine sarcoma virus (KiMSV)-transforming protein, p21ras, are unknown. Other studies have indicated that the mature form of p21 is distributed diffusely on the cytoplasmic face of the plasma membrane. However, after fixation without buffer washes, indirect immunofluorescent staini...

journal_title：Experimental cell research

authors： Myrdal SE,Auersperg N

更新日期：1985-08-01 00:00:00

abstract：:Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in t...

journal_title：Experimental cell research

authors： Iarovaia O,Razin SV,Linares-Cruz G,Sjakste N,Scherrer K

更新日期：2001-11-01 00:00:00

abstract：:Keratinocyte growth factor (KGF/FGF7) and fibroblast growth factor 10 (FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is...

journal_title：Experimental cell research

authors： Ceccarelli S,Cardinali G,Aspite N,Picardo M,Marchese C,Torrisi MR,Mancini P

更新日期：2007-05-15 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are multifunctional signaling molecules that have gained increasing interest in cancer research. To obtain a systematic view on BMP signaling in pancreatic cancer we first determined the mRNA expression levels of seven BMP ligands (BMP2-BMP8) and six BMP specific receptors in pancrea...

journal_title：Experimental cell research

authors： Virtanen S,Alarmo EL,Sandström S,Ampuja M,Kallioniemi A

更新日期：2011-09-10 00:00:00

abstract：:DNA topoisomerase (topo) I plays an important role in DNA metabolism by relieving the torsional restraints of DNA topology through ATP-independent single-strand DNA breakage. In the present study, we expressed human topo I in HeLa cells by fusing it to enhanced green fluorescent protein (EGFP). The EGFP-topo I fusion ...

journal_title：Experimental cell research

authors： Mo YY,Wang P,Beck WT

更新日期：2000-05-01 00:00:00

abstract：:The epigenetic states of key regulatory genes must be altered to drive cell fate decisions in differentiating cells. This process must be coupled, at least transiently, to the DNA replication machinery. Only a few genes, however, have been shown to require DNA replication for their activation or repression upon induct...

journal_title：Experimental cell research

authors： Bancescu DL,Glatt-Deeley H,Lalande M

更新日期：2004-08-01 00:00:00

abstract：:We had earlier demonstrated that a 46-kDa glycoprotein is involved in the differentiation of rat skeletal myoblasts. We now show that the binding of this glycoprotein to collagen and gelatin is disrupted by Arg-Gly-Asp (RGD) containing peptide but not by Arg-Gly-Glu (RGE). The former peptide also selectively elutes th...

journal_title：Experimental cell research

authors： Nandan D,Cates GA,Ball EH,Sanwal BD

更新日期：1988-11-01 00:00:00

abstract：:Caveolin-1 (Cav-1) is a principal structural protein of caveolae. Cav-1 has been implicated in cancer progression, but its precise functional roles in pituitary adenoma cells remain largely unclear. In this study, we evidenced that the level of cav-1 was elevated in the invasive pituitary adenoma. Cav-1 knockdown rest...

journal_title：Experimental cell research

authors： Yang W,Xu T,Qiu P,Xu G

更新日期：2018-06-01 00:00:00

abstract：BACKGROUND:Müller cell gliosis not only plays an important physiological role by maintaining retinal neuronal homeostasis but is also associated with multiple pathological events in the retina, including optic nerve crush (ONC) injury. Modulating Müller cell gliosis contributes to the creation of a permissive environme...

journal_title：Experimental cell research

authors： Li HJ,Sun ZL,Pan YB,Sun YY,Xu MH,Feng DF

更新日期：2019-02-15 00:00:00

abstract：:Collagen VI is a survival factor for skeletal muscle produced by endomysial cells and localized in connective tissue around muscle fibers. Mutations of its genes (COL6A1, COL6A2 and COL6A3) cause two muscular disorders, Bethlem myopathy and Ullrich disease. Expression of Collagen VI is highly dynamic during developmen...

journal_title：Experimental cell research

authors： Braghetta P,Ferrari A,Fabbro C,Bizzotto D,Volpin D,Bonaldo P,Bressan GM

更新日期：2008-11-15 00:00:00

abstract：:There are numerous mechanisms by which mammals respond to hypoxia. These include acute changes in pulmonary arterial tone due to smooth muscle cell contraction, acute increases in respiration triggered by the carotid body chemosensory cells, and chronic changes such as induction of red blood cell proliferation and ang...

journal_title：Experimental cell research

authors： McElroy GS,Chandel NS

更新日期：2017-07-15 00:00:00

abstract：:Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate w...

journal_title：Experimental cell research

authors： Bayley SA,Stones AJ,Smith CG

更新日期：1988-07-01 00:00:00

abstract：:A novel fluorescence-based method was developed to measure HIV envelope glycoprotein (env)-CD4-mediated cell fusion. This method measures the spread of a fluorescent dye as the cytosolic compartments of adjacent cells become contiguous upon cell-to-cell fusion. Calcein-labeled CD4+ Sup-T1 cells were seeded onto a mono...

journal_title：Experimental cell research

authors： Pine PS,Weaver JL,Oravecz T,Pall M,Ussery M,Aszalos A

更新日期：1998-04-10 00:00:00

abstract：:LMX1B is a LIM-homeodomain transcription factor essential for development. Putative LMX1B target genes have been identified through mouse gene targeting studies, but their identity as direct LMX1B targets remains hypothetical. We describe here the first molecular characterization of LMX1B target gene regulation. Micro...

journal_title：Experimental cell research

authors： Rascle A,Neumann T,Raschta AS,Neumann A,Heining E,Kastner J,Witzgall R

更新日期：2009-01-01 00:00:00

abstract：:Rap proteins (Rap1, Rap2a, b, c) are small molecular weight GTPases of the Ras family. Rap G proteins mediate diverse cellular events such as cell adhesion, proliferation, and gene activation through various signaling pathways. Activation of Rap signal is regulated tightly by several specific regulatory proteins inclu...

journal_title：Experimental cell research

authors： Minato N

更新日期：2013-09-10 00:00:00

abstract：:Phosphatidylinositol 3-kinase inhibitors have been shown to affect endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated beta(2)-adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endo...

journal_title：Experimental cell research

authors： Awwad HO,Iyer V,Rosenfeld JL,Millman EE,Foster E,Moore RH,Knoll BJ

更新日期：2007-07-15 00:00:00

abstract：:Herp (homocysteine-induced endoplasmic reticulum protein) is an ER-resident membrane protein, which has a ubiquitin-like domain at its N-terminus. Expression of Herp protein is up-regulated in response to ER stress, including homocysteine. Herp stabilizes neuronal Ca(2+) homeostasis and is involved in improving the ba...

journal_title：Experimental cell research

authors： Lenz B,Bleich S,Beutler S,Schlierf B,Schwager K,Reulbach U,Kornhuber J,Bönsch D

更新日期：2006-12-10 00:00:00

abstract：:The K63-linked ubiquitination of RIP1 coordinates survival/death homeostasis by driving transcription of genes downstream of RelA. Previously, we demonstrated that EGF-dependent RelA transactivation overcomes hypoxia-initiated apoptosis, yet the underlying mechanisms remain mysterious. We report here that UBXN1 defici...

journal_title：Experimental cell research

authors： Song YK,Hu BC,Xu L,Liu JQ,Chen X,Zheng Y,Chen MH,Wang JZ,Sun RH,Mo SJ

更新日期：2019-05-01 00:00:00

abstract：:Cells with and without hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity were used to examine the transfer of purine metabolites through the medium and via cell contacts. HGPRT- Chinese hamster and human fibroblasts were able to incorporate 3H-labeled purine metabolite(s) from medium in which mouse HGPRT...

journal_title：Experimental cell research

authors： Bols NC,Mosser DD,Boliska SA

更新日期：1987-11-01 00:00:00

abstract：:Although periodontal diseases are initiated by bacteria that colonize the tooth surface and gingival sulcus, the host response is believed to play an essential role in the breakdown of connective tissue and bone. Mitochondrial reactive oxygen species (mtROS) have been proposed to regulate the activation of the inflamm...

journal_title：Experimental cell research

authors： Li X,Wang X,Zheng M,Luan QX

更新日期：2016-09-10 00:00:00

abstract：:Adaptation to low oxygen conditions is essential for maintaining homeostasis and viability in oxygen-consuming multi-cellular tissues, including solid tumors. Central in these processes are the hypoxia-inducible transcription factors, HIF-1 and HIF-2, controlling genes involved in e.g. glucose metabolism and neovascul...

journal_title：Experimental cell research

authors： Löfstedt T,Fredlund E,Noguera R,Navarro S,Holmquist-Mengelbier L,Beckman S,Påhlman S,Axelson H

更新日期：2009-07-01 00:00:00

abstract：:In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...

journal_title：Experimental cell research

authors： Geiger D,Carpentier JL,Gorden P,Orci L

更新日期：1989-11-01 00:00:00

abstract：:We studied the expression of FREK (fibroblast growth factor receptor-like embryonic kinase), a new receptor recently cloned from quail embryo, during the differentiation of skeletal muscle satellite cells and epiphyseal growth-plate chondrocytes. Although FREK mRNA was expressed in both cell types, satellite cells exp...

journal_title：Experimental cell research

authors： Halevy O,Monsonego E,Marcelle C,Hodik V,Mett A,Pines M

更新日期：1994-06-01 00:00:00

abstract：:The growth of malignant melanoma cells is inhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA) while the growth of normal melanocytes is stimulated. We previously demonstrated that TPA inhibits the growth of Demel melanoma cells and leads to arrest at both at the G1/S and G2/M cell cycle transitions. To investigate...

journal_title：Experimental cell research

authors： Coppock DL,Buffolino P,Kopman C,Nathanson L

更新日期：1995-11-01 00:00:00

abstract：:In preliminary experiments it was established that the hypertrophic and hyperplastic responses of neonatal cardiac myocytes in culture were associated with enhanced expression of IGF-1 and IGF-1 receptors in these cells. Therefore, to determine the role of IGF-1 receptors on myocyte growth, cells were exposed to antis...

journal_title：Experimental cell research

authors： Kajstura J,Cheng W,Reiss K,Anversa P

更新日期：1994-12-01 00:00:00

abstract：:Fenretinide is an effective inducer of apoptosis in many malignancies but its precise mechanism(s) of action in the induction of apoptosis in neuroblastoma is unclear. To characterize fenretinide-induced apoptosis, neuroblastoma cell lines were treated with fenretinide and flow cytometry was used to measure apoptosis,...

journal_title：Experimental cell research

authors： Lovat PE,Ranalli M,Annichiarrico-Petruzzelli M,Bernassola F,Piacentini M,Malcolm AJ,Pearson AD,Melino G,Redfern CP

更新日期：2000-10-10 00:00:00

abstract：:Acetylcholine receptors (AChRs) of rat muscle cells grown in culture for 4 days were labeled with 125I-alpha-bungarotoxin and their degradation rate measured. Two AChR populations, a rapidly degrading one (Rr,t1/2 approximately 1 day) and a slowly degrading one (Rs, t1/2 approximately 4 days) were identified at a rati...

journal_title：Experimental cell research

authors： O'Malley JP,Rubin LL,Salpeter MM

更新日期：1993-09-01 00:00:00

abstract：:Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its lo...

journal_title：Experimental cell research

authors： Sato C,Nishizawa K,Nakayama T,Hirai R,Nakamura H

更新日期：1986-12-01 00:00:00