A semiautomated fluorescence-based cell-to-cell fusion assay for gp120-gp41 and CD4 expressing cells.

abstract：:The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase pro...

journal_title：Experimental cell research

authors： Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KH

更新日期：1983-12-01 00:00:00

abstract：:Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root gan...

journal_title：Experimental cell research

authors： Fedorov YV,Prudovsky IA,Yegorov YE,Zelenin AV

更新日期：1993-11-01 00:00:00

abstract：:Estrogen receptor-containing cells were identified in uteri of mature and senescent rats by means of quantitative autoradiography. Cell numbers and density were assessed in the various uterine layers. It was determined that despite decreases in receptor concentrations in endometrium, endometrial stroma, myometrium, an...

journal_title：Experimental cell research

authors： Han Z,Kokkonen GC,Roth GS

更新日期：1989-01-01 00:00:00

abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...

journal_title：Experimental cell research

authors： Li X,Lu H,Gu Y,Zhang X,Zhang G,Shi T,Chen W

更新日期：2020-01-01 00:00:00

abstract：:This study aims to clarify the role and molecular mechanism of dynamin-related protein 1 (Drp1)-mediated mitochondrial homeostasis in high glucose (HG)-induced endometrial cancer (EC). Normal endometrium and tumor tissues of EC patients with normal and HG levels were collected, and Drp1 and p-Drp1 expression levels we...

journal_title：Experimental cell research

authors： Guo J,Ye F,Jiang X,Guo H,Xie W,Zhang Y,Sheng X

更新日期：2020-04-01 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00

abstract：:Advanced stage neuroblastomas (NB) exhibit a tissue inhibitor of metalloproteinase (TIMP)-2/matrix metalloproteinase (MMP) imbalance, considered a prerequisite for MMP involvement in tumor progression in vivo. Human SH-SY5Y NB cells exhibit a similar TIMP-2/MMP imbalance that promotes in vitro invasive behavior that i...

journal_title：Experimental cell research

authors： Cappabianca L,Farina AR,Tacconelli A,Mantovani R,Gulino A,Mackay AR

更新日期：2003-06-10 00:00:00

abstract：:Human, Chinese hamster or Chinese hamster/human hybrid cytoplasts were fused with UV-irradiated xeroderma pigmentosum group A (XP-A) cells. Unscheduled DNA synthesis (UDS) of the XP-A nucleus was measured 0-2 and 2-4 h after seeding of the fused population. Human cytoplasts did correct the defect in the XP-A nucleus i...

journal_title：Experimental cell research

authors： Keijzer W,Stefanini M,Bootsma D,Verkerk A,Geurts van Kessel AH,Jongkind JF,Westerveld A

更新日期：1987-04-01 00:00:00

abstract：:Nod-like receptor pyrin domain-containing protein 6 (NLRP6) plays a key role in innate immunity, host defense and tumorigenesis. Our previous study has demonstrated the tumor suppressor role of NLRP6 in gastric cancer. In the present study, we explored the interaction protein of NLRP6 by Flag-tagged immunoprecipitatio...

journal_title：Experimental cell research

authors： Wang X,Wu X,Wang Q,Zhang Y,Wang C,Chen J

更新日期：2020-10-01 00:00:00

abstract：:The loss of expression of a single gene can revert normal tissue to a malignant phenotype. For example, while normal breast has high lumenal expression of CEACAM1, the majority of breast cancers exhibit the early loss of this gene with the concurrent loss of their lumenal phenotype. MCF7 cells that lack CEACAM1 expres...

journal_title：Experimental cell research

authors： Chean J,Chen CJ,Shively JE

更新日期：2017-10-01 00:00:00

abstract：:The migration and proliferation of vascular smooth muscle cells (vSMCs) are critical events in neointima formation during atherosclerosis and restenosis. The transcription factor nuclear factor of activated T-cells-isoform c1 (NFATc1) is regulated by atherogenic cytokines, and has been implicated in the migratory and ...

journal_title：Experimental cell research

authors： Chow W,Hou G,Bendeck MP

更新日期：2008-10-01 00:00:00

abstract：:Liver membrane glycoproteins with affinity for immobilized collagen type I were subjected to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution of the separated proteins. Electroeluted glycoproteins with ability to neutralize the inhibitory effect of anti-CollCA...

journal_title：Experimental cell research

authors： Gullberg D,Terracio L,Rubin K

更新日期：1988-04-01 00:00:00

abstract：:Proliferation of human B- and T-lymphoid cell lines including Raji and Akata cells was found to be arrested at the G1 stage in the cell cycle by dimethyl sulfoxide (DMSO). The G1 arrest by DMSO occurred gradually and was completed within 96 h after addition of 1.5% DMSO concomitantly with a decrease in growth rate. Pr...

journal_title：Experimental cell research

authors： Sawai M,Takase K,Teraoka H,Tsukada K

更新日期：1990-03-01 00:00:00

abstract：:The Drosophila sponge (spg)/CG31048 gene belongs to the dedicator of cytokinesis (DOCK) family genes that are conserved in a wide variety of species. DOCK family members are known as DOCK1-DOCK11 in mammals. Although DOCK1 and DOCK2 involve neurite elongation and immunocyte differentiation, respectively, the functions...

journal_title：Experimental cell research

authors： Eguchi K,Yoshioka Y,Yoshida H,Morishita K,Miyata S,Hiai H,Yamaguchi M

更新日期：2013-08-15 00:00:00

abstract：:Multiple myeloma is characterized by the malignant growth of immunoglobulin producing plasma cells, predominantly in the bone marrow. The effects of primary human mesenchymal stromal cells on the differentiation phenotype of multiple myeloma cells were studied by co-culture experiments. The incubation of multiple myel...

journal_title：Experimental cell research

authors： Dezorella N,Pevsner-Fischer M,Deutsch V,Kay S,Baron S,Stern R,Tavor S,Nagler A,Naparstek E,Zipori D,Katz BZ

更新日期：2009-07-01 00:00:00

abstract：:We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. Th...

journal_title：Experimental cell research

authors： Keherly MJ,Papaconstantinou J,McCombs JL

更新日期：1993-04-01 00:00:00

abstract：:The distribution of rDNA and the uptake of tritiated uridine was investigated in nucleoli of human Sertoli cells. The nucleolar components in these cells are spatially arranged in a highly ordered and invariable way and can be recognized in both light and electron microscopy. The pattern of distribution of rDNA and th...

journal_title：Experimental cell research

authors： Wachtler F,Hartung M,Devictor M,Wiegant J,Stahl A,Schwarzacher HG

更新日期：1989-09-01 00:00:00

abstract：:A monoclonal rat IgM antibody (4C9) raised against F9 embryonal carcinoma cells reacted with fucosyl residues in poly-N-acetyllactosamine-type large carbohydrates of these cells (embryoglycan). The chemical properties and distribution of the antigen resembled those of SSEA-1. The monoclonal antibody was found to inhib...

journal_title：Experimental cell research

authors： Nomoto S,Muramatsu H,Ozawa M,Suganuma T,Tashiro M,Muramatsu T

更新日期：1986-05-01 00:00:00

abstract：:Cellular senescence is an irreversible growth phase characteristic of normal cells. We have found that human senescent fibroblasts can be induced to undergo programmed cell death (apoptosis) by ceramide, TNF-alpha, or okadaic acid. The most profound effects were induced by TNF-alpha and okadaic acid treatment. In the ...

journal_title：Experimental cell research

authors： DeJesus V,Rios I,Davis C,Chen Y,Calhoun D,Zakeri Z,Hubbard K

更新日期：2002-03-10 00:00:00

abstract：:The long non-coding RNAs (lncRNAs) have gained much attention due to its essential roles in molecular regulation. As one of the classic lncRNAs, H19 is strongly expressed during embryogenesis and plays a crucial biological function during development. Mesenchymal stem cells (MSCs) are an ideal cell source for tissue e...

journal_title：Experimental cell research

authors： Liu Y,Li G,Zhang JF

更新日期：2017-11-15 00:00:00

abstract：:Studies performed over the past five years have demonstrated that there are two major cell-intrinsic pathways for inducing apoptosis, one that begins with ligation of cell surface death receptors and another that involves mitochondrial release of cytochrome c. Several reports have suggested that anticancer drugs kill ...

journal_title：Experimental cell research

authors： Kaufmann SH,Earnshaw WC

更新日期：2000-04-10 00:00:00

abstract：:In this communication, we have studied a 72,000 mol w (p72) host protein which reacts with a mouse monoclonal antibody (PAb6) directed against antigenic determinants on the Simian virus 40 (SV40) large T antigen protein that map 5' of 0.42 map units on the viral genome. The p72 protein is an abundant basic (pI greater...

journal_title：Experimental cell research

authors： Ullrich SJ,Appella E,Mercer WE

更新日期：1988-10-01 00:00:00

abstract：:Regulating the entry of secretory and membrane proteins into the ER is shown to be an important physiological process, in terms of controlling quantity, localization and therefore function of target proteins. However, few small molecule modulators are available to pharmacologically regulate translocation of a specific...

journal_title：Experimental cell research

authors： Kim J,Choi I,Park JY,Kang SW

更新日期：2013-08-01 00:00:00

abstract：:The rate-limiting step in melanogenesis is catalyzed by tyrosinase, a multifunctional enzyme encoded by the albino locus. We have previously reported that depletion of protein kinase C by long-term treatment of B16 mouse melanoma cells with phorbol dibutyrate (PDBu) prevented cell density-dependent melanogenesis. This...

journal_title：Experimental cell research

authors： Mahalingam H,Watanabe A,Tachibana M,Niles RM

更新日期：1997-11-25 00:00:00

abstract：:Nitric oxide (NO) markedly induces cardiomyocyte (CM) differentiation of embryonic stem (ES) cells. Here we examined the role of the Ca(2+) signaling in the NO-induced CM differentiation of mouse ES cells. We found that NO induced intracellular Ca(2+) increases in ES cells in a dose-dependent manner, and application o...

journal_title：Experimental cell research

authors： Wei W,Huang W,Yue J

更新日期：2016-08-01 00:00:00

abstract：:CEACAM1-4S (carcinoembryonic antigen-related cell adhesion molecule 1) is a type I membrane protein with a short (12-amino acid) cytoplasmic tail. Wild type CEACAM1-4S-transfected MCF7 cells form glands with lumena when grown in 3D culture, while null mutations of two putative phosphorylation sites (T457A and S459A) i...

journal_title：Experimental cell research

authors： Chen CJ,Nguyen T,Shively JE

更新日期：2010-02-15 00:00:00

abstract：:We report here the first direct observation of chemotaxis to EGF by rat mammary carcinoma cells. When exposed to a gradient of EGF diffusing from a micropipette, MTLn3 cells displayed typical ameboid chemotaxis, extending a lamellipod-like protrusion and moving toward the pipette. Using a homogeneous upshift in EGF to...

journal_title：Experimental cell research

authors： Bailly M,Yan L,Whitesides GM,Condeelis JS,Segall JE

更新日期：1998-06-15 00:00:00

abstract：:Pevonedistat is a potent, selective, first-in-class NEDD8 activating enzyme inhibitor. It is now under multiple clinical trials that investigate its anticancer effect against solid tumors and leukemia. ATP-binding cassette (ABC) transporters are membrane proteins that are involved in mediating multidrug resistance (MD...

journal_title：Experimental cell research

authors： Wei LY,Wu ZX,Yang Y,Zhao M,Ma XY,Li JS,Yang DH,Chen ZS,Fan YF

更新日期：2020-03-15 00:00:00

abstract：:The p70 ribosomal S6 kinase (S6K1) is rapidly activated following growth factor stimulation of quiescent fibroblasts and inhibition of this enzyme results in a G(1) arrest. Phosphorylation of the ribosomal S6 protein by S6K1 regulates the translation of both ribosomal proteins and initiation factors, leading to an inc...

journal_title：Experimental cell research

authors： Zhang H,Hoff H,Marinucci T,Cristofalo VJ,Sell C

更新日期：2000-08-25 00:00:00

abstract：:The laminins consist of at least 11 polypeptides (5 alpha-chains, 3 beta-chains, and 3 gamma-chains) specific to basement membranes. Here we investigate the biological activity associated with the G domain of the newly identified laminin alpha5-chain using 113 overlapping synthetic peptides (positions 2679-3635). Usin...

journal_title：Experimental cell research

authors： Makino M,Okazaki I,Kasai S,Nishi N,Bougaeva M,Weeks BS,Otaka A,Nielsen PK,Yamada Y,Nomizu M

更新日期：2002-07-01 00:00:00