Abstract:
:The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase protein level changes in parallel with activity. Although the K-isozyme is the primary form of pyruvate kinase expressed, some M-type subunit is also present and both forms undergo an increase in specific activity. In addition, pyruvate kinase expression is also elevated by adding cAMP analogues with theophylline, butyrate or conditioned media. This increased level of expression is hypothesized to be a secondary event associated with a differentiation-like-induced expression of the mast cell phenotype.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KHdoi
10.1016/0014-4827(83)90367-1subject
Has Abstractpub_date
1983-12-01 00:00:00pages
565-75issue
2eissn
0014-4827issn
1090-2422pii
0014-4827(83)90367-1journal_volume
149pub_type
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