Identification of cell binding sites in the laminin alpha5-chain G domain.

abstract：:The interfacial energy of the non-attached to substrate cell surface was analysed in tumor cell variants of the K-1735 melanoma and UV-2237 fibrosarcoma series, which exhibit distinct metastatic phenotypes. The highly metastatic cell variants exhibited a two-fold increase in the ability to form rapid cell substrate in...

journal_title：Experimental cell research

authors： Reich S,Rosin H,Levy M,Karkash R,Raz A

更新日期：1984-08-01 00:00:00

abstract：:The germinal vesicle (GV) of Xenopus laevis is an enormous nucleus that contains 18 giant lampbrush chromosomes and thousands of inclusions. The inclusions are primarily of three types: approximately 1500 extrachromosomal nucleoli, 50-100 Cajal bodies, and several thousand B-snurposomes, which correspond to speckles o...

journal_title：Experimental cell research

authors： Gall JG,Wu Z,Murphy C,Gao H

更新日期：2004-05-15 00:00:00

abstract：:Two SV40-transformed human cell lines, GM637, derived from a normal human subject, and GM5849, derived from a patient with ataxia-telangiectasia (A-T), were grown in increasing concentrations of the cytotoxic agent methotrexate (MTX). The GM637 line was naturally more resistant to methotrexate than was GM5849 and, ove...

journal_title：Experimental cell research

authors： Hahn P,Kapp LN,Painter RB

更新日期：1987-01-01 00:00:00

abstract：:Interferon beta is widely used as first-line treatment for relapsing remitting multiple sclerosis (RRMS). Several products are marketed world-wide, and biosimilar products are emerging. Interferon beta reduces relapse rates by about 1/3, and reduces the appearance of new MRI lesions by about 2/3, and some studies have...

journal_title：Experimental cell research

authors： Rudick RA,Goelz SE

更新日期：2011-05-15 00:00:00

abstract：:The enzyme activity hydrolysing diadenosine 5,5'-P1, P4-tetraphosphate (AP4A) was demonstrated in the embryonic extract of sea urchin. The enzyme activity was preferentially inhibited by ZnCl2 and by high concentrations of isobutylmethylxanthine, indicating that two types of the enzyme, (AP4A) hydrolase and non-specif...

journal_title：Experimental cell research

authors： Morioka M,Shimada H

更新日期：1987-03-01 00:00:00

abstract：BACKGROUND:The db/db mouse is an animal model of diabetes in which leptin receptor activity is deficient resulting accelerated cardiomyopathy when exposed to angiotensin (AT). Toll-like receptors 4 and 2 (TLR4, TLR2) are pattern recognition receptors, that recognize pathogen-associated molecular patterns and exacerbate...

journal_title：Experimental cell research

authors： Cohen K,Waldman M,Abraham NG,Laniado-Schwartzman M,Gurfield D,Aravot D,Arad M,Hochhauser E

更新日期：2017-01-01 00:00:00

abstract：:Human neutrophils developed long thin tubulovesicular extensions (cytonemes) upon adhesion to fibronectin-coated substrata, when spreading was blocked. We observed extension formation when neutrophils were plated to fibronectin-coated substrata in Na(+)-free extracellular medium or in the presence of drugs capable of ...

journal_title：Experimental cell research

authors： Galkina SI,Sud'ina GF,Ullrich V

更新日期：2001-06-10 00:00:00

abstract：:Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...

journal_title：Experimental cell research

authors： Nakahara H,Dennis JE,Bruder SP,Haynesworth SE,Lennon DP,Caplan AI

更新日期：1991-08-01 00:00:00

abstract：:Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduce...

journal_title：Experimental cell research

authors： Deslex S,Negrel R,Ailhaud G

更新日期：1987-01-01 00:00:00

abstract：:Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

journal_title：Experimental cell research

authors： De Wever B,Rooman RP,De Brabander M

更新日期：1990-01-01 00:00:00

abstract：:The biosynthesis of 6(R)-5,6,7,8-tetrahydrobiopterin (BH4) in murine erythroleukemia (MEL) cells is almost completely inhibited by 10 mM, 2,4-diamino-6-hydroxypyrimidine (DAHP), which targets GTP cyclohydrolase. The inhibition results in dephosphorylation of the retinoblastoma gene product, prolongation of the G1-phas...

journal_title：Experimental cell research

authors： Zhuo S,Fan S,Kaufman S

更新日期：1996-01-10 00:00:00

abstract：:The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported th...

journal_title：Experimental cell research

authors： Nöth U,Tuli R,Seghatoleslami R,Howard M,Shah A,Hall DJ,Hickok NJ,Tuan RS

更新日期：2003-11-15 00:00:00

abstract：:A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...

journal_title：Experimental cell research

authors： Schmidt-Zachmann MS,Hügle B,Scheer U,Franke WW

更新日期：1984-08-01 00:00:00

abstract：:Tau pathology in Alzheimer's disease (AD) includes hyperphosphorylation and truncation of tau. Phosphorylation at S422 is found to suppress truncation of tau at D421 that leading to the generation of ΔTau. However, the interrelation between hyperphosphorylation and generation of ΔTau in AD remains elusive. In current ...

journal_title：Experimental cell research

authors： Cao L,Liang Y,Liu Y,Xu Y,Wan W,Zhu C

更新日期：2018-09-01 00:00:00

abstract：:The results of Phase I/II clinical trials indicate that ricin A-chain-containing immunotoxins cause vascular leak syndrome, characterized by hypoalbuminemia with resultant weight gain and edema. Vascular leak syndrome may be a dose-limiting factor during treatment with ricin A-chain-containing immunotoxins. In this re...

journal_title：Experimental cell research

authors： Soler-Rodríguez AM,Ghetie MA,Oppenheimer-Marks N,Uhr JW,Vitetta ES

更新日期：1993-06-01 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microsc...

journal_title：Experimental cell research

authors： Krause S,Fakan S,Weis K,Wahle E

更新日期：1994-09-01 00:00:00

abstract：:We generated four monoclonal antibodies (mAbs) specific for human alpha 3 integrin (VLA-3 alpha subunit). All of them were found to induce homotypic cell aggregation of HT1080 fibrosarcoma and SN12C renal carcinoma cells, both of which express high levels of alpha 3 integrin. The antibodies also induced the cell aggre...

journal_title：Experimental cell research

authors： Takeuchi K,Tsuji T,Hakomori S,Irimura T

更新日期：1994-03-01 00:00:00

abstract：:In the naturally synchronous mitosis of the syncytial plasmodium of Physarum polycephalum, the nucleolus disintegrates in prophase, releasing a large amount of ribosomal RNA. Using biotinylated rDNA probes, we studied by high-resolution in situ hybridization the behavior of this nucleolar RNA throughout mitosis. Our r...

journal_title：Experimental cell research

authors： Pierron G,Puvion-Dutilleul F

更新日期：1993-10-01 00:00:00

abstract：:We recently observed a 25-fold increase in the activity of ornithine decarboxylase (ODC) 6 h after treatment of G1-synchronized CTLL-2 cells with interleukin-2 (IL-2). Here we show that the ODC mRNA content increased in parallel with the ODC activity during the first hours of stimulation with IL-2, resulting in a 25-f...

journal_title：Experimental cell research

authors： Legraverend C,Potter A,Hölttä E,Alitalo K,Andersson LC

更新日期：1989-03-01 00:00:00

abstract：:This study was designed to elucidate the mechanisms involved in elevated cell death arising from an altered endogenous oxidant state. Increased levels of cell death were detected in cells lacking Gpx1 following the addition of exogenous H2O2. This increased apoptosis correlated with a down-regulation in the activation...

journal_title：Experimental cell research

authors： Taylor JM,Crack PJ,Gould JA,Ali U,Hertzog PJ,Iannello RC

更新日期：2004-11-01 00:00:00

abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

journal_title：Experimental cell research

authors： Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

更新日期：1996-12-15 00:00:00

abstract：:The 90-kDa heat shock protein, HSP90, is an abundant molecular chaperone which functions in cellular homeostasis in prokaryotes and eukaryotes. It is well known that HSP90 plays a critical and indispensable role in regulating cell growth through modulations of various signal transduction pathways, but its roles in cel...

journal_title：Experimental cell research

authors： Zhao C,Hashiguchi A,Kondoh K,Du W,Hata J,Yamada T

更新日期：2002-05-01 00:00:00

abstract：:The long non-coding RNAs (lncRNAs) have gained much attention due to its essential roles in molecular regulation. As one of the classic lncRNAs, H19 is strongly expressed during embryogenesis and plays a crucial biological function during development. Mesenchymal stem cells (MSCs) are an ideal cell source for tissue e...

journal_title：Experimental cell research

authors： Liu Y,Li G,Zhang JF

更新日期：2017-11-15 00:00:00

abstract：:Normal fibroblast subpopulations have differential surface expression of the GPI-linked raft protein Thy-1, which correlates with differences in cellular adhesion and migration in vitro. Thrombospondin-1 (TSP-1) induces an intermediate state of adhesion in fibroblasts and other cells which facilitates migration. TSP-1...

journal_title：Experimental cell research

authors： Rege TA,Pallero MA,Gomez C,Grenett HE,Murphy-Ullrich JE,Hagood JS

更新日期：2006-11-15 00:00:00

abstract：:Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nu...

journal_title：Experimental cell research

authors： Skare P,Kreivi JP,Bergström A,Karlsson R

更新日期：2003-05-15 00:00:00

abstract：:The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

journal_title：Experimental cell research

authors： Olsson AK,Nånberg E

更新日期：2001-04-15 00:00:00

abstract：:Translational control is an essential process in regulation of gene expression, which occurs at the initiation step performed by a number of translation initiation factor complexes. eIF3a (eIF3 p170) is the largest subunit of the eIF3 complex. eIF3a has been suggested to play roles in regulating translation of a subse...

journal_title：Experimental cell research

authors： Dong Z,Liu Z,Cui P,Pincheira R,Yang Y,Liu J,Zhang JT

更新日期：2009-07-01 00:00:00

abstract：:Extracellular matrix (ECM) has been reported to enhance epithelial cell attachment and proliferation as well as to induce differentiation in vitro. In an attempt to determine the benefits of culturing pancreatic islet cells on ECM, we studied the morphological and functional patterns of rat islet cells and an insulin-...

journal_title：Experimental cell research

authors： Thivolet CH,Chatelain P,Nicoloso H,Durand A,Bertrand J

更新日期：1985-08-01 00:00:00

abstract：:Spindle assembly is essential for the equal distribution of genetic material to the daughter cells during mitosis. The process of spindle assembly is complicated and involves multiple levels of molecular regulation. It is generally accepted that mitotic spindles are emanated from the centrosomes and are assembled in t...

journal_title：Experimental cell research

authors： Yang CP,Fan SS

更新日期：2008-10-15 00:00:00