Immunodetection of poly(A) binding protein II in the cell nucleus.

abstract：:The proline analogs cis-4-hydroxy-L-proline, 3,4-dehydro-L-proline, and 2-azetidinecarboxylic acid induced increases in the migration rate of retinal capillary endothelial cells, aortal endothelial cells, corneal endothelial cells, aortal smooth muscle cells, and retinal pericytes. cis-4-hydroxy-D-proline did not. The...

journal_title：Experimental cell research

authors： McAuslan BR,Reilly W,Hannan GN,Schindhelm K,Milthorpe B,Saur BA

更新日期：1988-06-01 00:00:00

abstract：:Ets variant gene 5 (ETV5) and glial cell-derived neurotrophic factor (GDNF) are produced in Sertoli cells and required for maintenance and self-renewal of spermatogonial stem cells (SSCs) in mice. Fibroblast growth factors (FGFs) have been reported to stimulate Etv5 mRNA expression, and FSH was shown to stimulate Gdnf...

journal_title：Experimental cell research

authors： Simon L,Ekman GC,Tyagi G,Hess RA,Murphy KM,Cooke PS

更新日期：2007-08-15 00:00:00

abstract：:We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were used to avoid frequent i...

journal_title：Experimental cell research

authors： Okumoto K,Bogaki A,Tateishi K,Tsukamoto T,Osumi T,Shimozawa N,Suzuki Y,Orii T,Fujiki Y

更新日期：1997-05-25 00:00:00

abstract：:Formation of the neural tube is the morphological hallmark for development of the embryonic central nervous system (CNS). Therefore, neural tube development is a crucial step in the neurulation process. Slit/Robo signaling was initially identified as a chemo-repellent that regulated axon growth cone elongation, but it...

journal_title：Experimental cell research

authors： Wang G,Li Y,Wang XY,Han Z,Chuai M,Wang LJ,Ho Lee KK,Geng JG,Yang X

更新日期：2013-05-01 00:00:00

abstract：:Inorganic polyphosphate [Poly(P)] may represent a physiological source of phosphate and has the ability to induce bone differentiation in osteoblasts. We previously reported that cytokine-induced matrix metalloproteinase (MMP)-3 accelerates the proliferation of purified odontoblast-like cells. In this study, MMP-3 sma...

journal_title：Experimental cell research

authors： Ozeki N,Hase N,Yamaguchi H,Hiyama T,Kawai R,Kondo A,Nakata K,Mogi M

更新日期：2015-05-01 00:00:00

abstract：:Drebrin is an actin-binding protein which is expressed at highly levels in neurons. When introduced into fibroblasts, it has been known to bind to F-actin and to cause remodeling of F-actin. Here, we performed a domain analysis of the actin-binding and actin-remodeling activities of drebrin. Various fragments of drebr...

journal_title：Experimental cell research

authors： Hayashi K,Ishikawa R,Kawai-Hirai R,Takagi T,Taketomi A,Shirao T

更新日期：1999-12-15 00:00:00

abstract：:The stroma, the middle layer of the cornea, is a connective tissue making up most of the corneal thickness. The stromal extracellular matrix (ECM) consists of highly organised lamellae which are made up of tightly packed fibrils primarily composed of collagens type I and V. This layer is interspersed with keratocytes,...

journal_title：Experimental cell research

authors： Greene CA,Green CR,Dickinson ME,Johnson V,Sherwin T

更新日期：2016-09-10 00:00:00

abstract：:Polo-like kinases (Plks) are essential for progression through mitosis. The activity of these kinases peak during M phase and this activation has been attributed to phosphorylation. Kinases capable of activating Plks in vitro have been previously identified both in mammalian cells and in Xenopus laevis oocytes (SLK an...

journal_title：Experimental cell research

authors： Alves PS,Godinho SA,Tavares AA

更新日期：2006-02-01 00:00:00

abstract：:PKL12/STK16 protein is the first identified mammalian member of a ser/thr kinase subfamily that is conserved across several kingdoms, with a broad expression pattern in murine tissues and cell types. Endogenous STK16 subcellular localization was evaluated by indirect immunofluorescence in NIH/3T3 and NRK cells, demons...

journal_title：Experimental cell research

authors： Guinea B,Ligos JM,Laín de Lera T,Martín-Caballero J,Flores J,Gonzalez de la Peña M,García-Castro J,Bernad A

更新日期：2006-01-15 00:00:00

abstract：:Phosphatidylinositol 3-kinase inhibitors have been shown to affect endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated beta(2)-adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endo...

journal_title：Experimental cell research

authors： Awwad HO,Iyer V,Rosenfeld JL,Millman EE,Foster E,Moore RH,Knoll BJ

更新日期：2007-07-15 00:00:00

abstract：:ADP-ribosylation factor 3 (ARF3) is a member of the KRAS proto-oncogene, GTPase(Ras) super-family of guanine nucleotide-binding proteins that mediates Golgi-related mitosis, but its role in malignant cells is unclear. In the present study, we found that mRNA and protein expression of ARF3 is up-regulated in breast can...

journal_title：Experimental cell research

authors： Huang D,Pei Y,Dai C,Huang Y,Chen H,Chen X,Zhang X,Lin C,Wang H,Zhang R,Wan X,Wang L

更新日期：2019-11-15 00:00:00

abstract：:We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....

journal_title：Experimental cell research

authors： Pendergrass WR,Li Y,Jiang D,Fei RG,Wolf NS

更新日期：1995-04-01 00:00:00

abstract：:The stimulation of human tumor cells overexpressing epidermal growth factor receptor (EGFR) with EGF enhances tumor development and malignancy. Therefore, compounds that modulate the EGF-mediated signal inducing apoptosis in EGFR-overexpressing cells would represent a new class of antitumor drug and might be useful in...

journal_title：Experimental cell research

authors： Yoshimoto Y,Imoto M

更新日期：2002-09-10 00:00:00

abstract：:Growth and survival of tumors at a site of metastasis involve interactions with stromal cells in the surrounding environment. Stromal cells aid tumor cell growth by producing cytokines as well as by modifying the environment surrounding the tumor through modulation of the extracellular matrix (ECM). Small leucine-rich...

journal_title：Experimental cell research

authors： Coulson-Thomas VJ,Gesteira TF,Coulson-Thomas YM,Vicente CM,Tersariol IL,Nader HB,Toma L

更新日期：2010-11-15 00:00:00

abstract：:Mutations in PKHD1 (polycystic kidney and hepatic disease gene 1) gene cause the autosomal recessive polycystic kidney disease (ARPKD). Fibrocystin/polyductin (FPC), encoded by PKHD1, is a membrane-associated receptor-like protein. Although it is widely accepted that cystogenesis is mostly due to aberrant cell prolife...

journal_title：Experimental cell research

authors： Sun L,Wang S,Hu C,Zhang X

更新日期：2011-04-15 00:00:00

abstract：:Hematopoiesis is the hierarchical process in which all lineages of blood cells are produced by self-renewing hematopoietic stem cells (HSCs) in the bone marrow (BM). While the regulatory factors that maintain proper HSC function and lineage output under normal conditions are well understood, significantly less is know...

journal_title：Experimental cell research

authors： Mirantes C,Passegué E,Pietras EM

更新日期：2014-12-10 00:00:00

abstract：:Micro(mi)RNAs play important and varied roles in tumorigenesis; however, the full repertoire of miRNAs that affect cancer cell growth is not known. In this study, an miRNA library was screened to identify those that affect the growth of A549 tumor cells. Among 300 miRNAs, miR-28-5p, -323-5p, -510-5p, -552-3p, and -608...

journal_title：Experimental cell research

authors： Choi YC,Yoon S,Byun Y,Lee G,Kee H,Jeong Y,Yoon J,Baek K

更新日期：2015-12-10 00:00:00

abstract：:Plasmid 6.4 kbp DNA, 14 kbp DNA, lambda phage particles, all of which contained herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene, or IgM molecules, were mixed with erythrocyte membranes and treated with neutral detergent. The transparent mixture was diluted with phosphate-buffered saline (PBS), followed ...

journal_title：Experimental cell research

authors： Sugawa H,Uchida T,Yoneda Y,Ishiura M,Okada Y

更新日期：1985-08-01 00:00:00

abstract：:Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of ...

journal_title：Experimental cell research

authors： Hirako Y,Yonemoto Y,Yamauchi T,Nishizawa Y,Kawamoto Y,Owaribe K

更新日期：2014-06-10 00:00:00

abstract：:The high level and intranuclear location of the cellular phosphoprotein p53 are usually regarded as invariant features of SV40-transformed fibroblast lines. During the development of improved methods for immunocytochemical detection of p53 using SVA31 E7 mouse fibroblasts, we have observed unexpected and marked variat...

journal_title：Experimental cell research

authors： Davis D,Wynford-Thomas D

更新日期：1986-09-01 00:00:00

abstract：:Dominant mutations in presenilin1 (PS1) and presenilin2 (PS2) are a major cause of early-onset Alzheimer's disease. In this report we analyze the expression of the zebrafish presenilin1 (Psen1) and presenilin2 (Psen2) proteins during embryogenesis. We demonstrate that Psen1 and Psen2 holoproteins are relatively abunda...

journal_title：Experimental cell research

authors： Nornes S,Groth C,Camp E,Ey P,Lardelli M

更新日期：2003-09-10 00:00:00

abstract：:Durations of S-phase (Ts) and total cell cycle times (Tc) were measured from the peripheral blood (PB) and bone marrow aspirates (BM) of five patients with acute nonlymphocytic leukemia (ANLL). Intravenous bromodeoxyuridine (BrdU) was used as the first label for S-phase cells and a monoclonal anti-BrdU antibody was us...

journal_title：Experimental cell research

authors： Raza A,Yasin Z,Grande C

更新日期：1988-05-01 00:00:00

abstract：:Natural BH3-memitic (-)-gossypol shows promising antitumor efficacy in several kinds of cancer. However, our previous studies have demonstrated that protective autophagy decreases the drug sensitivities of Bcl-2 inhibitors in hepatocellular carcinoma (HCC) cells. In the present study, we are the first to report that H...

journal_title：Experimental cell research

authors： Wang B,Chen L,Ni Z,Dai X,Qin L,Wu Y,Li X,Xu L,Lian J,He F

更新日期：2014-11-01 00:00:00

abstract：:Repeated injury to the stratum corneum of mammalian skin (caused by friction, soaps, or organic solvents) elicits hyperkeratosis and epidermal thickening. Functionally, these changes serve to restore the cutaneous barrier and protect the organism. To better understand the molecular and cellular basis of this response,...

journal_title：Experimental cell research

authors： Ajani G,Sato N,Mack JA,Maytin EV

更新日期：2007-08-15 00:00:00

abstract：:At Day 17 of in ovo development, chondrocyte hypertrophy including synthesis of collagen X takes place in a limited region within the cranial part of chick embryo sternum. Here we analyze in suspension culture the differences in response to single growth factors of chondrocytes derived from the cranial part versus cel...

journal_title：Experimental cell research

authors： Böhme K,Winterhalter KH,Bruckner P

更新日期：1995-01-01 00:00:00

abstract：:Whey Acidic Protein (WAP) gene expression is specific to the mammary gland and regulated by lactogenic hormones to peak during lactation. It differs markedly from the more constitutive expression of the two flanking genes, Ramp3 and Tbrg4. Our results show that the tight regulation of WAP gene expression parallels var...

journal_title：Experimental cell research

authors： Montazer-Torbati MB,Hue-Beauvais C,Droineau S,Ballester M,Coant N,Aujean E,Petitbarat M,Rijnkels M,Devinoy E

更新日期：2008-03-10 00:00:00

abstract：:Time-lapse microcinematography was used to trace the migration and subsequent fate of daughter pairs from single myogenic stem cells. The generation times of siblings which divide are closely correlated (r, 0.73) compared with randomly selected cells (r, 0.059), suggesting the more or less equal partition of stem cell...

journal_title：Experimental cell research

authors： Konigsberg IR,Pfister KK

更新日期：1986-11-01 00:00:00

abstract：UNLABELLED:The success of hepatocyte transplantation has been limited by the low efficiency of transplanted cell integration into liver parenchyma. Human fetal hepatic progenitor cells (hepatoblasts) engraft more effectively than adult hepatocytes in mouse livers. However, the signals required for their integration are...

journal_title：Experimental cell research

authors： Delgado JP,Vanneaux V,Branger J,Touboul T,Sentilhes L,Mainot S,Lainas P,Leclerc P,Uzan G,Mahieu-Caputo D,Weber A

更新日期：2009-11-15 00:00:00

abstract：:Celiac disease (CD) is a multisystemic autoimmune inflammation of the intestinal tract induced by wheat gluten and related cereals in HLA-DQ2/8 positive individuals. An essential role in the pathogenesis of CD is played by a fraction of the peptic-tryptic digest of gluten, Frazer's Fraction (FF). Here, we investigate ...

journal_title：Experimental cell research

authors： Reinke Y,Zimmer KP,Naim HY

更新日期：2009-11-15 00:00:00

abstract：:In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for human regulatory subunits RII alpha RII beta of the cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies with several antibodies directed against RII alpha and RII beta show that these ...

journal_title：Experimental cell research

authors： Keryer G,Rios RM,Landmark BF,Skalhegg B,Lohmann SM,Bornens M

更新日期：1993-02-01 00:00:00