Abstract：
:Time-lapse microcinematography was used to trace the migration and subsequent fate of daughter pairs from single myogenic stem cells. The generation times of siblings which divide are closely correlated (r, 0.73) compared with randomly selected cells (r, 0.059), suggesting the more or less equal partition of stem cell components. The commitment to differentiative expression, however, is not inherited and must occur following stem cell division. Within the combined group in which at least one sibling fused, the ratio of pairs in which both fuse to pairs in which one divides and one fuses is 21:18. X2 for this ratio (compared with 1:1) is 0.103 indicating that it is just as likely that one daughter fuses and the other divides as that both fuse. We see no evidence of an intrinsic mitotic clock. If there were, one would expect that both or neither daughter would differentiate, depending on whether or not the 'clock' had run out for a particular stem cell.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Konigsberg IR,Pfister KKdoi
10.1016/0014-4827(86)90204-1subject
Has Abstractpub_date
1986-11-01 00:00:00pages
63-74issue
1eissn
0014-4827issn
1090-2422journal_volume
167pub_type
杂志文章abstract：:The effect of SK&F 96365 (1-(beta-[3-(4-methoxyphenyl)propoxyl]-4- methoxyphenethyl)-1H-imidazole hydrochloride), a recently synthesized inhibitor of receptor-mediated calcium entry, was investigated on human hematopoietic cell lines. We found that treatment of the T-cell leukemia line Jurkat with SK&F 96365 inhibited...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90103-f
更新日期：1992-10-01 00:00:00
abstract：:Cytotoxic T lymphocyte (CTL)-mediated lysis is accompanied by fragmentation of target cell DNA into an oligonucleosome ladder, a hallmark of apoptosis. Is this a fortuitous coincidence, or could CTL be inducing lysis by activation of the suicide signal? In this report we demonstrate that CTL-mediated target cell death...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1150
更新日期：1993-06-01 00:00:00
abstract：:A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxicall...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90204-2
更新日期：1988-04-01 00:00:00
abstract：:The bioflavonoid quercetin is a dietary anticancer chemical that is capable of inducing apoptosis in tumor cells. Although the activity of quercetin is believed to be due to its antioxidative properties, it has recently been suggested that quercetin also has prooxidant activities, which might effect cytotoxicity direc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00410-5
更新日期：2003-12-10 00:00:00
abstract：:Fibroblast migration is a central process in skin wound healing, which requires the coordination of several types of growth factors. bFGF, a well-known fibroblast growth factor (FGF), is able to accelerate fibroblast migration; however, the underlying mechanism of bFGF regulation fibroblast migration remains unclear. ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.03.054
更新日期：2017-06-15 00:00:00
abstract：:The 37 kDa/67 kDa laminin receptor (LRP/LR) is over-expressed in tumor cells and has been implicated in several tumourigenic processes such as metastasis and telomerase activation, however, more importantly the focus of the present study is on the maintenance of cellular viability and the evasion of apoptosis. The aim...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.04.003
更新日期：2018-07-01 00:00:00
abstract：:Transforming growth factor beta (TGFbeta) can modulate the activity of various MAP kinases. However, how this pathway may mediate TGFbeta-induced malignant phenotypes remains elusive. We investigated the role of autocrine TGFbeta signaling through MAP kinases in the regulation of cell survival in breast carcinoma MCF-...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.02.016
更新日期：2007-05-01 00:00:00
abstract：:The relationship between Lamin B2 and tumor proliferation and migration is unclear. We explored the impact of Lamin B2 on non-small cell lung cancer (NSCLC) cells. Tissue microarray and immunohistochemistry were combined to evaluate Lamin B2 expression and its relationship with the clinicopathological factors found in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112090
更新日期：2020-08-15 00:00:00
abstract：:Raver2 was originally identified as a member of the hnRNP family through database searches revealing three N-terminal RNA recognition motifs (RRMs) bearing highest sequence identity in the RNP sequences to the related hnRNP Raver1. Outside the RRM region, both Raver proteins are quite divergent in sequence except for ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.11.023
更新日期：2010-04-01 00:00:00
abstract：:In many eukaryotic organisms, initiation of DNA synthesis is associated with a major control point within the cell cycle and reflects the commitment of the cell to the DNA replication-division portion of the cell cycle. In Paramecium, the timing of DNA synthesis initiation is established prior to fission during the pr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90215-6
更新日期：1986-11-01 00:00:00
abstract：:Abdominal aortic aneurysms (AAA) are characterized by matrix remodeling, elastin degradation, absence of nitric oxide (NO) signaling, and inflammation, influencing smooth muscle cell (SMC) phenotype and gene expression. Little is known about the biomolecular release and intrinsic biomechanics of human AAA-SMCs. NO del...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111589
更新日期：2019-11-01 00:00:00
abstract：:The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90593-7
更新日期：1984-02-01 00:00:00
abstract：:Nod-like receptor pyrin domain-containing protein 6 (NLRP6) plays a key role in innate immunity, host defense and tumorigenesis. Our previous study has demonstrated the tumor suppressor role of NLRP6 in gastric cancer. In the present study, we explored the interaction protein of NLRP6 by Flag-tagged immunoprecipitatio...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112177
更新日期：2020-10-01 00:00:00
abstract：:The expression level of DNA polymerase beta was determined in various mouse tissues. Northern blot hybridization analysis using rat cDNA as a probe revealed that the mRNA of about 1.5 kb for this enzyme is present in all kinds of tissues examined, but its content widely varies among tissues; the most abundant DNA poly...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90191-2
更新日期：1989-03-01 00:00:00
abstract：:The biosynthesis of 6(R)-5,6,7,8-tetrahydrobiopterin (BH4) in murine erythroleukemia (MEL) cells is almost completely inhibited by 10 mM, 2,4-diamino-6-hydroxypyrimidine (DAHP), which targets GTP cyclohydrolase. The inhibition results in dephosphorylation of the retinoblastoma gene product, prolongation of the G1-phas...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0021
更新日期：1996-01-10 00:00:00
abstract：:Rhabdomyosarcoma (RMS) are soft-tissue sarcoma commonly encountered in childhood. RMS cells can acquire invasive behavior and form metastases. The metastatic dissemination implicates many proteases among which are mu-calpain and m-calpain. Study of calpain expression and activity underline the deregulation of calpain ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.02.017
更新日期：2010-05-15 00:00:00
abstract：:Transient activation of the signal transducers and activators of transcription (STAT) proteins in response to growth hormone (GH) and other type II cytokines plays a pivotal role on specific gene transcription. The negative regulation of STATs seems to be exerted at the GH receptor (GHR)/Janus Kinase (JAK) complex and...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.11.017
更新日期：2004-03-10 00:00:00
abstract：:We have studied the morphological alterations of the lysosomal compartment in rat hepatocytes following intraperitoneal administration of leupeptin, using electron microscopy and cytochemical techniques. At 30 min after the injection, autophagic vacuoles (autophagosomes and autolysosomes), containing cytoplasmic organ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90436-6
更新日期：1983-03-01 00:00:00
abstract：:Scavenger receptor class A (SR-A) facilitates the development of atherosclerosis, which might be due to its role in the uptake of modified low-density lipoproteins. However, the receptor is also suggested to be important for cell adhesion, thereby potentially influencing the residence time of cells in vivo. Using SR-A...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4530
更新日期：1999-07-10 00:00:00
abstract：:The puromycin analog N6,N6-dimethyladenine (6-dimethylaminopurine or 6-DMAP) was found to inhibit meiosis reinitiation in starfish oocytes stimulated by the natural hormone 1-methyladenine. Increasing concentrations of this agent delayed and eventually blocked germinal vesicle breakdown. They were found to be effectiv...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90121-8
更新日期：1988-05-01 00:00:00
abstract：:The process of DNA synthesis in normal and endoreduplicating mammalian cells are very similar. Both types of chromosomes are replicated in defined units termed chromosomal replicons, and in the same sequences along their lengths. The sister chromosomes of the diplochromosomes are replicated synchronously in identical ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90148-9
更新日期：1983-07-01 00:00:00
abstract：:The level of integrin alpha(v)beta3 and its ligand osteopontin (OPN) has been directly correlated to tumorigenicity of melanoma and other cancer cells. We have previously shown an increase in pp(60c-Src) kinase activity associated with integrin alpha(v)beta3 in melanoma cells (M21) treated with soluble OPN. pp(60c-Src...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.03.022
更新日期：2006-07-15 00:00:00
abstract：:Prosomes constitute the multicatalytic proteinase (MCP) core of the 26S proteasomes, but were first observed as subcomplexes of untranslated mRNP; this suggests that they play a putative role in the control of protein biosynthesis in addition to their catabolic enzymatic function. In previous investigations it was sho...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3561
更新日期：1997-05-25 00:00:00
abstract：:The kidney's glomerular filtration barrier consists of two cells-podocytes and endothelial cells-and the glomerular basement membrane (GBM), a specialized extracellular matrix that lies between them. Like all basement membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteog...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2012.02.031
更新日期：2012-05-15 00:00:00
abstract：:Although osteocalcin is the most abundant noncollagenous protein in bone, its role remains undefined. Recent studies have reported diametrically opposing responses in the vitamin D regulation of the mouse vs the human and rat osteocalcin genes. The aim of this study was to increase the understanding of these differenc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4912
更新日期：2000-08-01 00:00:00
abstract：:This study deals with the effects of hyper- and hypo-osmotic media on the ultrastructure of four different types of cells and tissues: rat pheochromocytoma cells of line PC12, mouse Ehrlich ascites tumor cells, rat kidney cortex and intestine. Application of hyper-osmotic conditions induces in the nuclear compartment ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90240-x
更新日期：1985-09-01 00:00:00
abstract：:Seven continuous cell lines have been established in vitro from lethal embryos produced by the female sterile mutant mh 1182 of Drosophila melanogaster. Six lines show haploid metaphases. Karyotype analysis revealed a high level of aneuploid cells with frequent chromosome fragments. In three lines, haploid cells were ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90371-9
更新日期：1984-03-01 00:00:00
abstract：:Hyaluronan binding protein (HABP1), located on human chromosome 17p13.3, was identified and characterized as being involved in cellular signaling from our laboratory. Here, we demonstrate that HABP1 expression in Schizosaccharomyces pombe induces growth inhibition, morphological abnormalities like elongation, multinuc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.05.025
更新日期：2005-10-01 00:00:00
abstract：:Durations of S-phase (Ts) and total cell cycle times (Tc) were measured from the peripheral blood (PB) and bone marrow aspirates (BM) of five patients with acute nonlymphocytic leukemia (ANLL). Intravenous bromodeoxyuridine (BrdU) was used as the first label for S-phase cells and a monoclonal anti-BrdU antibody was us...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90116-4
更新日期：1988-05-01 00:00:00
abstract：:We have investigated the effects of the lysosomotropic amines, ammonium chloride and chloroquine, on the delivery of fluid-phase pinocytic tracers to lysosomes in Chinese hamster ovary (CHO) cells. In preliminary experiments, 15 mM ammonium chloride and 0.1 mM chloroquine were found to be sufficient to give maximal pr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90192-9
更新日期：1988-04-01 00:00:00
