DNA fragmentation induced by cytotoxic T lymphocytes can result in target cell death.


:Cytotoxic T lymphocyte (CTL)-mediated lysis is accompanied by fragmentation of target cell DNA into an oligonucleosome ladder, a hallmark of apoptosis. Is this a fortuitous coincidence, or could CTL be inducing lysis by activation of the suicide signal? In this report we demonstrate that CTL-mediated target cell death can be blocked with the drug aurintricarboxylic acid (ATA). The abrogation of death correlates with the inhibition of DNA fragmentation. While ATA prevented DNA fragmentation, it failed to significantly alter protein, RNA, or DNA synthesis in the cell lines over the dose range used. In addition, there was no inhibition of cell-cell interaction or granule exocytosis during CTL-mediated killing. ATA also significantly inhibited the cytolysis and DNA fragmentation mediated by isolated cytolytic granules, as well as the granular protein fragmentin. We developed an assay in which target cells could be separated from CTL after binding and programming for lysis. Once they had received the "kiss of death," target cells could be rescued from lysis (as indicated by inhibition of DNA fragmentation and increased target cell viability) by treatment with ATA. These results suggest that ATA blocks target cell death by inhibition of DNA fragmentation, and further, that chromatin degradation is a cause rather than a result of cell death in CTL-mediated lysis.


Exp Cell Res


Helgason CD,Shi L,Greenberg AH,Shi Y,Bromley P,Cotter TG,Green DR,Bleackley RC




Has Abstract


1993-06-01 00:00:00














  • Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments.

    abstract::Phosphorylation of types III and IV intermediate filaments (IFs) is known to regulate their organization and function. Phosphorylation of the amino-terminal head domain sites on types III and IV IF proteins plays a key role in the assembly/disassembly of IF subunits into 10 nm filaments, and influences the phosphoryla...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Sihag RK,Inagaki M,Yamaguchi T,Shea TB,Pant HC

    更新日期:2007-06-10 00:00:00

  • Functional characterization of the microtubule-binding and -destabilizing domains of CPAP and d-SAS-4.

    abstract::We previously identified a novel centrosomal protein CPAP, which carries a 112-residue motif that is essential for microtubule destabilization. In this report, we define both the microtubule (MT) binding and destabilizing domains in human CPAP and analyze the mutations that affect its MT-destabilizing activity. Analys...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hsu WB,Hung LY,Tang CJ,Su CL,Chang Y,Tang TK

    更新日期:2008-08-15 00:00:00

  • Immunopurification of a sarcomeric junctional protein complex containing GAPDH.

    abstract::We have isolated a monoclonal antibody, P4B2, which localizes to multiple anchorage junctions, namely, a subset of focal adhesions, the Z-disk of muscle, and neuromuscular junctions. Immunopurification of the antigen to this antibody from chicken brain tissue yielded a complex of three prominent proteins with mobiliti...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: McDonald KA,Muschler J,Horwitz AF

    更新日期:1998-09-15 00:00:00

  • Disruption of IGF-1R signaling increases TRAIL-induced apoptosis: a new potential therapy for the treatment of melanoma.

    abstract::Resistance of cancer cells to apoptosis is dependent on a balance of multiple genetic and epigenetic mechanisms, which up-regulate efficacy of the surviving growth factor-receptor signaling pathways and suppress death-receptor signaling pathways. The Insulin-like Growth Factor-1 Receptor (IGF-1R) signaling pathway is ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Karasic TB,Hei TK,Ivanov VN

    更新日期:2010-07-15 00:00:00

  • Molecular functions of brain expressed X-linked 2 (BEX2) in malignancies.

    abstract::Over the last decade there has been growing evidence that Brain Expressed X-Linked 2 (BEX2) has a significant role in the process of carcinogenesis. Collectively, available studies suggest a pro-oncogenic function for this gene in multiple malignancies, including breast, colorectal and hepatocellular cancers in additi...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Naderi A

    更新日期:2019-03-15 00:00:00

  • Differentiation-linked expression of prothymosin alpha gene in human myeloid leukemic cells.

    abstract::Prothymosin alpha (ProT alpha) is a nuclear protein related to cell proliferation. Its gene is highly activated during postnatal development at stages containing many proliferating but also differentiating cells. In this report, a study on ProT alpha gene expression during differentiation of human myeloid leukemic (HL...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Dosil M,Alvarez-Fernández L,Gómez-Márquez J

    更新日期:1993-01-01 00:00:00

  • MPF amplification in Xenopus oocyte extracts depends on a two-step activation of cdc25 phosphatase.

    abstract::The activation of Cdc2 kinase induces the entry into M-phase of all eukaryotic cells. We have developed a cell-free system prepared from prophase-arrested Xenopus oocytes to analyze the mechanism initiating the all-or-none activation of Cdc2 kinase. Inhibition of phosphatase 2A, the major okadaic acid-sensitive Ser/Th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Karaïskou A,Cayla X,Haccard O,Jessus C,Ozon R

    更新日期:1998-11-01 00:00:00

  • Developmental control of Presenilin1 expression, endoproteolysis, and interaction in zebrafish embryos.

    abstract::Dominant mutations in presenilin1 (PS1) and presenilin2 (PS2) are a major cause of early-onset Alzheimer's disease. In this report we analyze the expression of the zebrafish presenilin1 (Psen1) and presenilin2 (Psen2) proteins during embryogenesis. We demonstrate that Psen1 and Psen2 holoproteins are relatively abunda...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nornes S,Groth C,Camp E,Ey P,Lardelli M

    更新日期:2003-09-10 00:00:00

  • Developmental regulation of elongation factor-1 delta in sea urchin suggests appearance of a mechanism for alternative poly(A) site selection in gastrulae.

    abstract::Elongation factor-1 delta gene expression was analyzed during sea urchin development. EF-1 delta mRNA is present as a single 2.7-kb transcript in unfertilized eggs and in rapidly dividing cleavage stage embryos. It decreases rapidly 6 h after fertilization and then reappears at the gastrula stage as two transcripts of...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Delalande C,Monnier A,Minella O,Genevière AM,Mulner-Lorillon O,Bellé R,Cormier P

    更新日期:1998-07-10 00:00:00

  • Overexpression of cyclin D1 blocks proliferation of normal diploid fibroblasts.

    abstract::Human cyclin D1 forms complexes with several Cdks, with proliferating cell nuclear antigen, and with a recently discovered 21-kDa inhibitor of Cdk activity. Substrates for cyclin D1/Cdks have not been identified in vivo, but it has been proposed that the D class of cyclins might play a role in regulating the activity ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Atadja P,Wong H,Veillete C,Riabowol K

    更新日期:1995-04-01 00:00:00

  • Sudden volume changes of the lumen of inverted thyroid follicles in suspension cultures. Use in estimation of rates of water flux through thyroid epithelium.

    abstract::Inverted thyroid follicles in suspension culture were observed using dark-field illumination and time-lapse video enhancement techniques. The lumens of many follicles undergo episodes of rapid shrinkage followed by relatively slow dilation. The extent of shrinkage ranged from 10 to 40% of the initial diameter. The dec...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nitsch L,Wollman SH

    更新日期:1986-01-01 00:00:00

  • KIN1 and KIN2 protein kinases localize to the cytoplasmic face of the yeast plasma membrane.

    abstract::Two genes, KIN1 and KIN2, were isolated in Saccharomyces cerevisiae by their homology to the protein kinase family of viral oncogenes. In previous studies, we identified the yeast KIN1 gene product (pp145KIN1) and KIN2 gene product (pp145KIN2) as 145 kDa phosphoproteins with serine/threonine protein kinase activity. W...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tibbetts M,Donovan M,Roe S,Stiltner AM,Hammond CI

    更新日期:1994-07-01 00:00:00

  • The over-expression of HAS2, Hyal-2 and CD44 is implicated in the invasiveness of breast cancer.

    abstract::Within tumors there appears to be an intricate balance between hyaluronan (HA) synthesis and degradation where the invading edges display increased HA metabolism. The metabolism of HA has not been characterized in breast cancer cell lines; therefore, this study quantitatively identifies and characterizes the enzymes r...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Udabage L,Brownlee GR,Nilsson SK,Brown TJ

    更新日期:2005-10-15 00:00:00

  • Human Kupffer cell recognition and phagocytosis of apoptotic peripheral blood lymphocytes.

    abstract::Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L

    更新日期:1996-04-10 00:00:00

  • Distinct functions for Rap1 signaling in vascular morphogenesis and dysfunction.

    abstract::Rap1 signaling is important for both major processes of vessel formation: vasculogenesis, or de novo vessel formation, and angiogenesis, sprouting of new vessels from pre-existing ones. We provide an overview of genetic studies in mice and zebrafish and discuss some of the proposed underlying mechanisms derived from c...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Chrzanowska-Wodnicka M

    更新日期:2013-09-10 00:00:00

  • Conditional inactivation of Brca1 in the mouse ovarian surface epithelium results in an increase in preneoplastic changes.

    abstract::Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Clark-Knowles KV,Garson K,Jonkers J,Vanderhyden BC

    更新日期:2007-01-01 00:00:00

  • Cell-substrate contact: another factor may influence transepithelial electrical resistance of cell layers cultured on permeable filters.

    abstract::Transepithelial resistance (TER) measurement has often been used to study the paracellular transport properties of epithelia grown on permeable filters, especially the barrier function of tight junctions. However, the TER value includes another source, the resistance caused by cell-substrate contact, that may give ris...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lo CM,Keese CR,Giaever I

    更新日期:1999-08-01 00:00:00

  • Plakophilin3 loss leads to an increase in lipocalin2 expression, which is required for tumour formation.

    abstract::An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To identify pathways downstream of PKP3 loss that are required for increased tumour formation, a gene expression analysis was performed, which demonstrated that the expression of lipocalin2 (LCN2) was elevated upon PKP3 loss and...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Basu S,Chaudhary N,Shah S,Braggs C,Sawant A,Vaz S,Thorat R,Gupta S,Dalal SN

    更新日期:2018-08-15 00:00:00

  • LIM-only protein FHL3 interacts with CDC25B2 phosphatase.

    abstract::LIM domain proteins are important regulators of the growth, determination, and differentiation of cells. In this report, FHL3 (human four-and-a-half LIM-only protein 3) is shown to interact with human phosphatase CDC25B, a cell cycle regulator involved in the control of G2/M. We found that this interaction was specifi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mils V,Lee SM,Joly W,Hang EW,Baldin V,Waye MM,Ducommun B,Tsui SK

    更新日期:2003-04-15 00:00:00

  • CD25-expressing Th17 cells mediate CD8+ T cell suppression in CTLA-4 dependent mechanisms in pancreatic ductal adenocarcinoma.

    abstract::The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lang C,Wang J,Chen L

    更新日期:2017-11-15 00:00:00

  • Pretreatment with oleic acid accelerates the entrance into the mitotic cycle of EGF-stimulated fibroblasts.

    abstract::We have previously demonstrated that pretreatment of several cell lines with cis-unsaturated fatty acids, like oleic acid, blocks epidermal growth factor (EGF)-induced early ionic signals, and in particular the [Ca2+]i rise. In the present work we show that this blockade does not alter EGF-stimulated cellular prolifer...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zugaza JL,Casabiell XA,Bokser L,Eiras A,Beiras A,Casanueva FF

    更新日期:1995-07-01 00:00:00

  • Effect of tunicamycin on cell cycle progression in budding yeast.

    abstract::Tunicamycin, an inhibitor of one of the earliest steps in the synthesis of N-linked oligosaccharides, prevents bud formation and growth in Saccharomyces cerevisiae cells that are either growing exponentially or recovering from different cell cycle arrests at start. Analysis of tunicamycin-treated cells by flow microfl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Vai M,Popolo L,Alberghina L

    更新日期:1987-08-01 00:00:00

  • Cytochemical localization of adenylate cyclase in bovine cumulus-oocyte complexes.

    abstract::The ultrastructural localization of adenylate cyclase was studied in bovine cumulus-oocyte complexes. Adenylate cyclase was observed on the plasma membrane of the oocyte and occasionally on the plasma membrane of cumulus cells. The cytochemical observations presented demonstrate that there is more adenylate cyclase in...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kuyt JR,Kruip TA,de Jong-Brink M

    更新日期:1988-01-01 00:00:00

  • NLRP3 inflammasome inhibition attenuates cisplatin-induced renal fibrosis by decreasing oxidative stress and inflammation.

    abstract:BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

    更新日期:2019-10-01 00:00:00

  • Characterization and response of newly developed high-grade glioma cultures to the tyrosine kinase inhibitors, erlotinib, gefitinib and imatinib.

    abstract::High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

    更新日期:2012-03-10 00:00:00

  • The role of HGF on invasive properties and repopulation potential of human fetal hepatic progenitor cells.

    abstract:UNLABELLED:The success of hepatocyte transplantation has been limited by the low efficiency of transplanted cell integration into liver parenchyma. Human fetal hepatic progenitor cells (hepatoblasts) engraft more effectively than adult hepatocytes in mouse livers. However, the signals required for their integration are...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Delgado JP,Vanneaux V,Branger J,Touboul T,Sentilhes L,Mainot S,Lainas P,Leclerc P,Uzan G,Mahieu-Caputo D,Weber A

    更新日期:2009-11-15 00:00:00

  • Betulinic acid derivatives can protect human Müller cells from glutamate-induced oxidative stress.

    abstract::Müller cells are the predominant retinal glial cells. One of the key roles of Müller cells is in the uptake of the neurotransmitter glutamate and in its conversion to glutamine. Müller cell dysfunction due to oxidative stress elicited by high glutamate concentrations can lead to toxicity, which promote the pathogenesi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cheng Z,Zhang T,Zheng J,Ding W,Wang Y,Li Y,Zhu L,Murray M,Zhou F

    更新日期:2019-10-01 00:00:00

  • Constitutively active BMP type I receptors transduce BMP-2 signals without the ligand in C2C12 myoblasts.

    abstract::Bone morphogenetic protein-2 (BMP-2), a member of transforming growth factor-beta superfamily, inhibits the terminal differentiation of C2C12 myoblasts and changes their differentiation pathway into cells expressing osteoblast phenotypes such as alkaline phosphatase (ALP) activity and osteocalcin production (Katagiri ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Akiyama S,Katagiri T,Namiki M,Yamaji N,Yamamoto N,Miyama K,Shibuya H,Ueno N,Wozney JM,Suda T

    更新日期:1997-09-15 00:00:00

  • Possible role of deep tubular invaginations of the plasma membrane in MHC-I trafficking.

    abstract::Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma memb...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Massol RH,Larsen JE,Kirchhausen T

    更新日期:2005-05-15 00:00:00

  • RelA/NF-kappaB transcription factor associates with alpha-actinin-4.

    abstract::The NF-kappaB/RelA family of transcription factors regulates inducible transcription of a large number of genes in response to diverse stimuli. Little is known, however, about the location of NF-kappaB in the cytoplasm and the transport mechanism to the nucleus. We found that NF-kappaB is associated with the actin-bin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Babakov VN,Petukhova OA,Turoverova LV,Kropacheva IV,Tentler DG,Bolshakova AV,Podolskaya EP,Magnusson KE,Pinaev GP

    更新日期:2008-03-10 00:00:00