The ING1b tumor suppressor facilitates nucleotide excision repair by promoting chromatin accessibility to XPA.


:ING1b is the most studied ING family protein and perhaps the most ubiquitously and abundantly expressed. This protein is involved in the regulation of various biological functions ranging from senescence, cell cycle arrest, apoptosis, to DNA repair. ING1b is upregulated by UV irradiation and enhances the removal of bulky nucleic acid photoproducts. In this study, we provide evidence that ING1b mediates nucleotide excision repair by facilitating the access to damaged nucleosomal DNA. We demonstrate that ING1b is not recruited to UV-induced DNA lesions but enhances nucleotide excision repair only in XPC-proficient cells, implying an essential role in early steps of the 'access, repair, restore' model. We also find that ING1b alters histone acetylation dynamics upon exposure to UV radiation and induces chromatin relaxation in microccocal nuclease digestion assay, revealing that ING1b may allow better access to nucleotide excision repair machinery. More importantly, ING1b associates with chromatin in a UV-inducible manner and facilitates DNA access to nucleotide excision repair factor XPA. Furthermore, depletion of the endogenous ING1b results to the sensitization of cells at S-phase to UV irradiation. Taken together, these observations establish a role of ING1b acting as a chromatin accessibility factor for DNA damage recognition proteins upon genotoxic injury.


Exp Cell Res


Kuo WH,Wang Y,Wong RP,Campos EI,Li G




Has Abstract


2007-05-01 00:00:00














  • siRNA - Mediated LRP/LR knock-down reduces cellular viability of malignant melanoma cells through the activation of apoptotic caspases.

    abstract::The 37 kDa/67 kDa laminin receptor (LRP/LR) is over-expressed in tumor cells and has been implicated in several tumourigenic processes such as metastasis and telomerase activation, however, more importantly the focus of the present study is on the maintenance of cellular viability and the evasion of apoptosis. The aim...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rebelo TM,Vania L,Ferreira E,Weiss SFT

    更新日期:2018-07-01 00:00:00

  • Identification of sequence determinants of human nuclear dUTPase isoform localization.

    abstract::dUTP nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate and is the central regulator of cellular dUTP pools. Nuclear (DUT-N) and mitochondrial (DUT-M) isoforms of the protein have been identified in humans and arise from the same gene by the alternative use of 5' exons. Recently, ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tinkelenberg BA,Fazzone W,Lynch FJ,Ladner RD

    更新日期:2003-07-01 00:00:00

  • Profilin I colocalizes with speckles and Cajal bodies: a possible role in pre-mRNA splicing.

    abstract::Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nu...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Skare P,Kreivi JP,Bergström A,Karlsson R

    更新日期:2003-05-15 00:00:00

  • 1,25-dihydroxyvitamin D3 induces biphasic NF-kappaB responses during HL-60 leukemia cells differentiation through protein induction and PI3K/Akt-dependent phosphorylation/degradation of IkappaB.

    abstract::1,25-dihydroxyvitamin D(3) (VD(3)) induces differentiation in a number of leukemia cell lines and under various conditions is able to either stimulate or inhibit nuclear factor kappa B (NF-kappaB) activity. Here we report a time-dependent biphasic regulation of NF-kappaB in VD(3)-treated HL-60 leukemia cells. After VD...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tse AK,Wan CK,Shen XL,Zhu GY,Cheung HY,Yang M,Fong WF

    更新日期:2007-05-01 00:00:00

  • Cyclic AMP in ovarian cancer cells both inhibits proliferation and increases c-KIT expression.

    abstract::C-KIT encodes a tyrosine kinase receptor (KIT) that, when activated by its ligand (KL), stimulates proliferation, differentiation, migration, and survival. Greater than 70% of epithelial ovarian cancers coexpress c-KIT and KL. C-KIT and KL expression levels have been shown to be up-regulated by cAMP in some cell types...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shaw TJ,Keszthelyi EJ,Tonary AM,Cada M,Vanderhyden BC

    更新日期:2002-02-01 00:00:00

  • Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells.

    abstract::Inorganic polyphosphate [Poly(P)] may represent a physiological source of phosphate and has the ability to induce bone differentiation in osteoblasts. We previously reported that cytokine-induced matrix metalloproteinase (MMP)-3 accelerates the proliferation of purified odontoblast-like cells. In this study, MMP-3 sma...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ozeki N,Hase N,Yamaguchi H,Hiyama T,Kawai R,Kondo A,Nakata K,Mogi M

    更新日期:2015-05-01 00:00:00

  • Caspase-mediated apoptosis in AK-5 tumor cells: a cell-free study using peptide inhibitors and antisense strategy.

    abstract::An in vitro system has been employed to study the apoptotic mechanisms in the AK-5 tumor which is a spontaneously regressing rat histiocytoma. Cytosolic extracts of tumor cells primed for apoptosis using dexamethasone and immune serum from tumor-regressing animals were able to induce apoptosis in intact nuclei and rep...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Anjum R,Khar A

    更新日期:1997-11-01 00:00:00

  • SIRT1 and microRNAs: The role in breast, lung and prostate cancers.

    abstract::Breast cancer and prostate cancer are the most common malignant tumors in female and men, respectively. Furthermore, lung cancer is the leading cause of cancer deaths worldwide. It is an emergency to develop a powerful strategy to treat these threatening cancers more effectively, because of low efficacy and high rates...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Karbasforooshan H,Roohbakhsh A,Karimi G

    更新日期:2018-06-01 00:00:00

  • Endocytosed epidermal growth factor (EGF) receptors contribute to the EGF-mediated growth arrest in A431 cells by inducing a sustained increase in p21/CIP1.

    abstract::We investigated the ability of endocytosed activated epidermal growth factor receptors (EGFR) to induce expression of the cyclin-interacting protein p21/CIP1 in A431 cells. Transforming growth factor alpha (TGFalpha) and EGF both induced tyrosine phosphorylation, induction of p21/CIP1, and thereby inhibition of DNA sy...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Skarpen E,Johannessen LE,Bjerk K,Fasteng H,Guren TK,Lindeman B,Thoresen GH,Christoffersen T,Stang E,Huitfeldt HS,Madshus IH

    更新日期:1998-08-25 00:00:00

  • PC13 embryonal carcinoma cells produce a heparin-binding growth factor.

    abstract::A polypeptide growth factor has been isolated from serum-free medium conditioned by mouse PC13 embryonal carcinoma cells, which is strongly mitogenic for Swiss 3T3 fibroblasts. On a CM-2-SW high-performance liquid chromatography cation-exchange column at low pH, this growth factor elutes at a salt concentration very c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: van Veggel JH,van Oostwaard TM,de Laat SW,van Zoelen EJ

    更新日期:1987-04-01 00:00:00

  • Identification and localization of a novel nucleolar protein of high molecular weight by a monoclonal antibody.

    abstract::A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schmidt-Zachmann MS,Hügle B,Scheer U,Franke WW

    更新日期:1984-08-01 00:00:00

  • The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b.

    abstract::The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Simpson F,Lammerts van Bueren K,Butterfield N,Bennetts JS,Bowles J,Adolphe C,Simms LA,Young J,Walsh MD,Leggett B,Fowles LF,Wicking C

    更新日期:2006-01-01 00:00:00

  • MicroRNA-92a -mediated endothelial to mesenchymal transition controls vein graft neointimal lesion formation.

    abstract:PURPOSE:Long-term failure of vein grafts due to neointimal hyperplasia remains an important problem in coronary artery bypass graft surgery. Endothelial to mesenchymal transition (EndMT) contributes to vein graft vascular remodeling. However, there is little study on microRNA-mediated EndMT contributions to neointimal ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhong CM,Li S,Wang XW,Chen D,Jiang ZL,Zhang C,He XJ,Huang C,Jiang YJ,Wu QC

    更新日期:2021-01-01 00:00:00

  • Characterization of p87C3G, a novel, truncated C3G isoform that is overexpressed in chronic myeloid leukemia and interacts with Bcr-Abl.

    abstract::A novel C3G isoform, designated p87C3G, lacking the most amino terminal region of the cognate protein has been found to be overexpressed in two CML cell lines, K562 and Boff 210, both expressing Bcr-Abl p210. p87C3G expression is also highly augmented in patients diagnosed with chronic myeloid leukemia (CML) Ph+, in c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gutiérrez-Berzal J,Castellano E,Martín-Encabo S,Gutiérrez-Cianca N,Hernández JM,Santos E,Guerrero C

    更新日期:2006-04-01 00:00:00

  • Factor Xa stimulates fibroblast procollagen production, proliferation, and calcium signaling via PAR1 activation.

    abstract::Fibroblast proliferation and procollagen production are central features of tissue repair and fibrosis. In addition to its role in blood clotting, the coagulation cascade proteinase thrombin can contribute to tissue repair by stimulating fibroblasts via proteolytic activation of proteinase-activated receptor-1 (PAR1)....

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Blanc-Brude OP,Archer F,Leoni P,Derian C,Bolsover S,Laurent GJ,Chambers RC

    更新日期:2005-03-10 00:00:00

  • Jun B expression is regulated differently by three mitogenic pathways in thyrocytes.

    abstract::In dog thyrocytes in primary culture, thyrotropin (TSH), acting through cyclic AMP, induces proliferation and differentiation expression, while tetradecanoylphorbol acetate (TPA) or epidermal growth factor (EGF) induces proliferation and dedifferentiation. In this work, we have investigated the regulation of mRNA expr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pirson I,Dumont JE

    更新日期:1994-10-01 00:00:00

  • Signal transduction in endothelial cells by the angiogenesis inhibitor histidine-rich glycoprotein targets focal adhesions.

    abstract::Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lee C,Dixelius J,Thulin A,Kawamura H,Claesson-Welsh L,Olsson AK

    更新日期:2006-08-01 00:00:00

  • The cytoplasmic pH, ATP content and total protein synthesis rate during heat-shock protein inducing treatments in yeast.

    abstract::In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Weitzel G,Pilatus U,Rensing L

    更新日期:1987-05-01 00:00:00

  • Altered structure of ribosomal RNA transcription units in hamster cells after DRB treatment.

    abstract::The adenosine analog 5,6 dichloro-1-beta-ribofuranosyl benzimidazole (DRB) inhibits specifically mRNA production in eukaryotic cells, with concomitant alterations in pre-rRNA processing and nucleolar ultrastructure. We have focused our analysis on the effects of drug treatment on rRNA transcription through observation...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Puvion-Dutilleul F,Nicoloso M,Bachellerie JP

    更新日期:1983-06-01 00:00:00

  • Cyclosporin A is a differential inhibitor of eukaryotic RNA polymerases.

    abstract::In order to investigate the molecular mechanism of biological action of the drug cyclosporin A (CsA), we have studied its effect on eukaryotic RNA polymerase activities in two systems: (a) in vitro transcription; (b) injection into frog oocytes. The electron microscopic transcription R-loop method [1] has been used to...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Brack C,Mattaj IW,Gautschi J,Cammisuli S

    更新日期:1984-04-01 00:00:00

  • Activation of cysteine proteases in cowpea plants during the hypersensitive response--a form of programmed cell death.

    abstract::There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: D'Silva I,Poirier GG,Heath MC

    更新日期:1998-12-15 00:00:00

  • High expression levels of the "erythroid/brain" type glucose transporter (GLUT1) in the basal cells of human eye conjunctiva and oral mucosa reconstituted in culture.

    abstract::The expression of the "erythroid/brain" type glucose transporter (GLUT1) seems to be a feature of "barrier" tissues, at least in humans. Recently, we reported that GLUT1 is highly expressed in the basal layers of either "authentic" human epidermis or human epidermis reconstituted in culture and that its expression see...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gherzi R,Melioli G,De Luca M,D'Agostino A,Guastella M,Traverso CE,D'Anna F,Franzi AT,Cancedda R

    更新日期:1991-07-01 00:00:00

  • Mammalian ChlR1 has a role in heterochromatin organization.

    abstract::The ChlR1 DNA helicase, encoded by DDX11 gene, which is responsible for Warsaw breakage syndrome (WABS), has a role in sister-chromatid cohesion. In this study, we show that human ChlR1 deficient cells exhibit abnormal heterochromatin organization. While constitutive heterochromatin is discretely localized at perinucl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Inoue A,Hyle J,Lechner MS,Lahti JM

    更新日期:2011-10-15 00:00:00

  • Down-regulation of insulin receptors is related to insulin internalization.

    abstract::In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Geiger D,Carpentier JL,Gorden P,Orci L

    更新日期:1989-11-01 00:00:00

  • Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells.

    abstract::Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription wa...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Egyhazi E,Pigon A,Chang JH,Ghaffari SH,Dreesen TD,Wellman SE,Case ST,Olson MO

    更新日期:1988-10-01 00:00:00

  • Emerging functions: diseases and animal models reshape our view of the cytoskeleton.

    abstract::Intermediate filaments (IFs), desmosomes, and their associates are built from multidomain proteins that form cytoskeletal scaffolds in the cytoplasm and the nucleus of vertebrate tissues. Mutations in more than 80 genes cause monogenic disorders that include severe skin fragility, myopathies, neurodegeneration, and pr...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Magin TM,Reichelt J,Hatzfeld M

    更新日期:2004-11-15 00:00:00

  • Endothelial nitric oxide synthase is segregated from caveolin-1 and localizes to the leading edge of migrating cells.

    abstract::The enzyme endothelial Nitric Oxide Synthase (eNOS) is involved in key physiological and pathological processes, including cell motility and apoptosis. It is widely believed that at the cell surface eNOS is localized in caveolae, where caveolin-1 negatively regulates its activity, however, there are still uncertaintie...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bulotta S,Cerullo A,Barsacchi R,De Palma C,Rotiroti D,Clementi E,Borgese N

    更新日期:2006-04-01 00:00:00

  • Disruption of the Golgi apparatus by brefeldin A inhibits the cytotoxicity of ricin, modeccin, and Pseudomonas toxin.

    abstract::We have studied the cytotoxicity of ricin in cells treated with brefeldin A (BFA), which dramatically disrupts the structure of the Golgi apparatus causing Golgi content and membrane to redistribute to the ER. BFA inhibits the cytotoxicity of ricin in Chinese hamster ovary, normal rat kidney, and Vero cells and abolis...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yoshida T,Chen CC,Zhang MS,Wu HC

    更新日期:1991-02-01 00:00:00

  • Differentiation of C2C12 myoblasts expressing lamin A mutated at a site responsible for Emery-Dreifuss muscular dystrophy is improved by inhibition of the MEK-ERK pathway and stimulation of the PI3-kinase pathway.

    abstract::Mutation R453W in A-type lamins, that are major nuclear envelope proteins, generates Emery-Dreifuss muscular dystrophy. We previously showed that mouse myoblasts expressing R453W-lamin A incompletely exit the cell cycle and differentiate into myocytes with a low level of multinucleation. Here we attempted to improve d...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Favreau C,Delbarre E,Courvalin JC,Buendia B

    更新日期:2008-04-01 00:00:00

  • Down-regulation of UDP-glucose dehydrogenase affects glycosaminoglycans synthesis and motility in HCT-8 colorectal carcinoma cells.

    abstract::UDP-glucose dehydrogenase (UGDH) catalyzes oxidation of UDP-glucose to yield UDP-glucuronic acid, a precursor of hyaluronic acid (HA) and other glycosaminoglycans (GAGs) in extracellular matrix. Although association of extracellular matrix with cell proliferation and migration has been well documented, the importance ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wang TP,Pan YR,Fu CY,Chang HY

    更新日期:2010-10-15 00:00:00