The ING1b tumor suppressor facilitates nucleotide excision repair by promoting chromatin accessibility to XPA.

Abstract:

:ING1b is the most studied ING family protein and perhaps the most ubiquitously and abundantly expressed. This protein is involved in the regulation of various biological functions ranging from senescence, cell cycle arrest, apoptosis, to DNA repair. ING1b is upregulated by UV irradiation and enhances the removal of bulky nucleic acid photoproducts. In this study, we provide evidence that ING1b mediates nucleotide excision repair by facilitating the access to damaged nucleosomal DNA. We demonstrate that ING1b is not recruited to UV-induced DNA lesions but enhances nucleotide excision repair only in XPC-proficient cells, implying an essential role in early steps of the 'access, repair, restore' model. We also find that ING1b alters histone acetylation dynamics upon exposure to UV radiation and induces chromatin relaxation in microccocal nuclease digestion assay, revealing that ING1b may allow better access to nucleotide excision repair machinery. More importantly, ING1b associates with chromatin in a UV-inducible manner and facilitates DNA access to nucleotide excision repair factor XPA. Furthermore, depletion of the endogenous ING1b results to the sensitization of cells at S-phase to UV irradiation. Taken together, these observations establish a role of ING1b acting as a chromatin accessibility factor for DNA damage recognition proteins upon genotoxic injury.

journal_name

Exp Cell Res

authors

Kuo WH,Wang Y,Wong RP,Campos EI,Li G

doi

10.1016/j.yexcr.2007.02.010

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

1628-38

issue

8

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(07)00074-2

journal_volume

313

pub_type

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