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Simultaneous cell death and desquamation of the embryonic diffusion barrier during epidermal development.

Abstract：

:The periderm is an epithelial layer covering the emerging epidermis in early embryogenesis of vertebrates. In the chicken embryo, an additional cellular layer, the subperiderm, occurs at later embryonic stages underneath the periderm. The questions arose what is the function of both epithelial layers and, as they are transitory structures, by which mechanism are they removed. By immunocytochemistry, the tight junction (TJ) proteins occludin and claudin-1 were localized in the periderm and in the subperiderm, and sites of close contact between adjacent cells were detected by electron microscopy. Using horseradish peroxidase (HRP) as tracer, these contacts were identified as tight junctions involved in the formation of the embryonic diffusion barrier. This barrier was lost by desquamation at the end of the embryonic period, when the cornified envelope of the emerging epidermis was formed. By TUNEL and DNA ladder assays, we detected simultaneous cell death in the periderm and the subperiderm shortly before hatching. The absence of caspases-3, -6, and -7 activity, key enzymes of apoptosis, and the lack of typical morphological criteria of apoptosis such as cell fragmentation or membrane blebbing point to a special form of programmed cell death (PCD) leading to the desquamation of the embryonic diffusion barrier.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Saathoff M,Blum B,Quast T,Kirfel G,Herzog V

doi

10.1016/j.yexcr.2004.06.009

keywords:

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

415-26

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014482704003398

journal_volume

299

pub_type

杂志文章

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