Abstract：
:Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondrocytes were used to study the endocytosis and turnover of CD44 and the effects of receptor occupancy on this turnover. Matrix-intact chondrocytes exhibit approximately a 6% internalization of cell surface CD44 by 4 h. Treatment with Streptomyces hyaluronidase to remove endogenous pericellular matrix increased internalization to approximately 20% of cell surface CD44 at 4 h. This turnover could be partially inhibited by the addition of exogenous hyaluronan to these matrix-depleted chondrocytes. Cell surface biotin-labeled CD44 was internalized by chondrocytes and this internalization was decreased in the presence of hyaluronan. Colocalization of internalized CD44 and fluorescein-labeled hyaluronan in intracellular vesicles correlates with the previous results of receptor-mediated endocytosis pathway for the degradation of hyaluronan by acid hydrolases. Taken together, our results indicate that CD44 is internalized by chondrocytes and that CD44 turnover is modulated by occupancy with hyaluronan.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Aguiar DJ,Knudson W,Knudson CBdoi
10.1006/excr.1999.4641keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
292-302issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(99)94641-4journal_volume
252pub_type
杂志文章abstract：:Rat myotubes in tissue culture form broad areas of close contact with the substrate. These areas often display two distinct, interdigitating sets of membrane domains. One, the "contact domain", is close to the substrate; the other, termed the "AChR domain", is further from the substrate and is rich in acetylcholine re...
journal_title：Experimental cell research
pub_type： 杂志文章
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abstract：:A portion of the DNA within intact nuclei of a spontaneously transformed Chinese hamster ovary cell line (CHO-Kl) is relatively resistant to digestion by pancreatic deoxyribonuclease, as compared to nuclei from primary cultures of Chinese hamster ovary fibroblasts. Treatment of CHO-Kl cells with derivatives of 3',5' c...
journal_title：Experimental cell research
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abstract：:Insulin-like growth factor I (IGF-I) promotes the motility of different cell types. We investigated the role of IGF-I receptor (IGF-IR) signaling in locomotion of MCF-7 breast cancer epithelial cells overexpressing the wild-type IGF-IR (MCF-7/IGF-IR). Stimulation of MCF-7/IGF-IR cells with 50 ng/ml IGF-I induced disru...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1999-08-25 00:00:00
abstract：:The 90-kDa heat shock protein, HSP90, is an abundant molecular chaperone which functions in cellular homeostasis in prokaryotes and eukaryotes. It is well known that HSP90 plays a critical and indispensable role in regulating cell growth through modulations of various signal transduction pathways, but its roles in cel...
journal_title：Experimental cell research
pub_type： 杂志文章
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abstract：:Wnt1 and -5a have been shown to modulate the proliferation and differentiation of midbrain dopaminergic (DA) neurons. However, it is not known whether other Wnts or which Frizzled (Fz) receptors are expressed in the developing midbrain. We found that 13 out of 19 Wnts, all 10 Fzs, and several intracellular Wnt signali...
journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2017-12-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2017-06-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2004-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4827(88)90472-7
更新日期：1988-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.03.042
更新日期：2017-05-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2014.07.016
更新日期：2014-11-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4865
更新日期：2000-05-25 00:00:00
abstract：:The casein kinase I (CKI) family consists of at least seven vertebrate genes, some of which can be alternatively spliced. Previously, we have studied the four splice variants of the chicken CKIalpha gene. The four proteins differ only by the presence or absence of two peptides, a 28-amino-acid "L" insert in the cataly...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5324
更新日期：2001-10-01 00:00:00
abstract：:The effect of the physiological vitamin D metabolite 24R, 25-dihydroxyvitamin D3 [24R,25(OH)2D3] on human osteoblastic cells was assessed. Physiological concentrations (10(-9)-10(-8) M) of 24R, 25(OH)2D3 significantly increased the cyclic guanosine 5'-monophosphate (cGMP) content in the human osteoblastic cells by app...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4189
更新日期：1998-10-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90056-x
更新日期：1986-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90220-9
更新日期：1984-12-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2014.02.028
更新日期：2014-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4012
更新日期：1998-05-01 00:00:00
abstract：:Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90105-4
更新日期：1991-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-05-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2018-09-15 00:00:00
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doi：10.1016/j.yexcr.2019.111776
更新日期：2020-02-01 00:00:00
abstract：:The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.12.017
更新日期：2008-04-01 00:00:00
abstract：:Our results showed that the expression of the src gene in chick embryo fibroblasts (CEF) released the density-dependent inhibition (DDI) of phosphate metabolism (phosphate uptake and phosphorylation of small organic compounds). With increasing cell density, phosphate metabolism decreased by 58% in normal CEF and, in c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90640-2
更新日期：1984-06-01 00:00:00
abstract：:This study aims to clarify the role and molecular mechanism of dynamin-related protein 1 (Drp1)-mediated mitochondrial homeostasis in high glucose (HG)-induced endometrial cancer (EC). Normal endometrium and tumor tissues of EC patients with normal and HG levels were collected, and Drp1 and p-Drp1 expression levels we...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.111880
更新日期：2020-04-01 00:00:00
abstract：PURPOSE:Owing to the late diagnosis and frequent metastasis, ovarian cancer (OC) exhibits a high mortality rate. The study was intended to figure out the function of long non-coding RNA (lncRNA) HCP5 in OC metastasis. METHODS:Microarray analysis was conducted to probe aberrantly expressed lncRNAs in OC tissues. Artifi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112129
更新日期：2020-09-01 00:00:00
