Four casein kinase I isoforms are differentially partitioned between nucleus and cytoplasm.

abstract：:Histone deacetylase inhibitors (HDACi) were recently identified as having significant clinical potential in reversing β-cell functional inhibition caused by inflammation, a shared precursor of Type 1 and Type 2 diabetes. However, HDACi are highly complex and little is known of their direct effect on important cell sec...

journal_title：Experimental cell research

authors： Tiernan AR,Champion JA,Sambanis A

更新日期：2015-01-01 00:00:00

abstract：:Leptomycin B (LMB) is a Streptomyces metabolite that inhibits nuclear export of the human immunodeficiency virus type 1 regulatory protein Rev at low nanomolar concentrations. Recently, LMB was shown to inhibit the function of CRM1, a receptor for the nuclear export signal (NES). Here we show evidence that LMB binds d...

journal_title：Experimental cell research

authors： Kudo N,Wolff B,Sekimoto T,Schreiner EP,Yoneda Y,Yanagida M,Horinouchi S,Yoshida M

更新日期：1998-08-01 00:00:00

abstract：:The K63-linked ubiquitination of RIP1 coordinates survival/death homeostasis by driving transcription of genes downstream of RelA. Previously, we demonstrated that EGF-dependent RelA transactivation overcomes hypoxia-initiated apoptosis, yet the underlying mechanisms remain mysterious. We report here that UBXN1 defici...

journal_title：Experimental cell research

authors： Song YK,Hu BC,Xu L,Liu JQ,Chen X,Zheng Y,Chen MH,Wang JZ,Sun RH,Mo SJ

更新日期：2019-05-01 00:00:00

abstract：:The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for m...

journal_title：Experimental cell research

authors： Tu J,Zhang B,Fang G,Chang W,Zhao Y

更新日期：2020-05-15 00:00:00

abstract：:Topoisomerase II is essential for cell proliferation and survival and has been a target of various anticancer drugs. ICRF-193 has long been used as a catalytic inhibitor to study the function of topoisomerase II. Here, we show that ICRF-193 treatment induces DNA damage signaling. Treatment with ICRF-193 induced G2 arr...

journal_title：Experimental cell research

authors： Park I,Avraham HK

更新日期：2006-07-01 00:00:00

abstract：:The activation of cdc2/cyclin B is the trigger for entry into mitosis. The mechanism of cdc2/cyclin B activation is complex, but the final step is the dephosphorylation of the Thr14 and Tyr15 residues on the cdc2 subunit, catalyzed by a member of the Cdc25 family of phosphatases. Cdc2/cyclin B1 accumulates at the cent...

journal_title：Experimental cell research

authors： De Souza CP,Ellem KA,Gabrielli BG

更新日期：2000-05-25 00:00:00

abstract：:Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a...

journal_title：Experimental cell research

authors： Segal-Bendirdjian E,Jacquemin-Sablon A

更新日期：1995-05-01 00:00:00

abstract：:Oxysterol-binding protein (OSBP)-related proteins (ORPs) are conserved lipid binding proteins found in organisms ranging from yeast to mammals. Recent findings have indicated that these proteins mainly localize to contact sites of 2 different membranous organelles. ORP6, a member of the ORP subfamily III, is one of th...

journal_title：Experimental cell research

authors： Mochizuki S,Miki H,Zhou R,Kido Y,Nishimura W,Kikuchi M,Noda Y

更新日期：2018-09-15 00:00:00

abstract：:MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockou...

journal_title：Experimental cell research

authors： Petri R,Malmevik J,Fasching L,Åkerblom M,Jakobsson J

更新日期：2014-02-01 00:00:00

abstract：:Plasmid 6.4 kbp DNA, 14 kbp DNA, lambda phage particles, all of which contained herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene, or IgM molecules, were mixed with erythrocyte membranes and treated with neutral detergent. The transparent mixture was diluted with phosphate-buffered saline (PBS), followed ...

journal_title：Experimental cell research

authors： Sugawa H,Uchida T,Yoneda Y,Ishiura M,Okada Y

更新日期：1985-08-01 00:00:00

abstract：:The mitochondrion imports and processes the vast majority of the proteins that constitute its structural elements and metabolic pathways. To study mitochondrial precursor processing in the context of the cellular environment, we employed the baculovirus expression system to overexpress the prototypical precursor prote...

journal_title：Experimental cell research

authors： Wright G,Reichenbecher V

更新日期：1999-02-01 00:00:00

abstract：:When mesenchymal cells from the early chick limb bud (stage 23-24) are plated at high cell density they spontaneously undergo chondrogenesis implicating extensive cell-to-cell interactions. In the past it has been shown that TGF-beta and vitamin D can influence this process and can stimulate chondrogenesis. Given the ...

journal_title：Experimental cell research

authors： Tsonis PA,Del Rio-Tsonis K,Millan JL,Wheelock MJ

更新日期：1994-08-01 00:00:00

abstract：:Current methods of monitoring insulin in culture are limited to soluble insulin (secretions or lysates) or synthetic gene reporter analyses. We present an insulin-specific cell enzyme-linked immunosorbent assay (cell-ELISA) to assess relative intracellular insulin protein content of adherent cultures, normalized to ce...

journal_title：Experimental cell research

authors： Andersen PL,Vermette P

更新日期：2016-09-10 00:00:00

abstract：:Activin A belongs to the superfamily of transforming growth factor beta (TGFβ) and is a critical regulatory cytokine in breast cancer and inflammation. However, the role of activin A in migration of breast cancer cells and immune cells was not well characterized. Here, a microfluidic device was used to examine the eff...

journal_title：Experimental cell research

authors： Xie D,Liu Z,Wu J,Feng W,Yang K,Deng J,Tian G,Santos S,Cui X,Lin F

更新日期：2017-08-01 00:00:00

abstract：:Sperm cells from control donors of proven fertility and men from barren couples were studied by conventional procedures, i.e., light microscopy as well as flow cytometry. Light microscopy analysis of semen included the measurement of spermatozoa concentration, morphology, and motility. All the men from barren couples ...

journal_title：Experimental cell research

authors： Troiano L,Granata AR,Cossarizza A,Kalashnikova G,Bianchi R,Pini G,Tropea F,Carani C,Franceschi C

更新日期：1998-06-15 00:00:00

abstract：:We have previously demonstrated that pretreatment of several cell lines with cis-unsaturated fatty acids, like oleic acid, blocks epidermal growth factor (EGF)-induced early ionic signals, and in particular the [Ca2+]i rise. In the present work we show that this blockade does not alter EGF-stimulated cellular prolifer...

journal_title：Experimental cell research

authors： Zugaza JL,Casabiell XA,Bokser L,Eiras A,Beiras A,Casanueva FF

更新日期：1995-07-01 00:00:00

abstract：:Rap1 signaling is important for both major processes of vessel formation: vasculogenesis, or de novo vessel formation, and angiogenesis, sprouting of new vessels from pre-existing ones. We provide an overview of genetic studies in mice and zebrafish and discuss some of the proposed underlying mechanisms derived from c...

journal_title：Experimental cell research

authors： Chrzanowska-Wodnicka M

更新日期：2013-09-10 00:00:00

abstract：:Bax-mediated apoptosis in neurons is involved in many pathologic conditions affecting the central nervous system, including degenerative diseases, stroke, and trauma. Two molecules belonging to the Bcl-2 family, Bcl-2 and Bcl-X(L), protect cells from Bax-induced apoptosis and show distinct expression patterns in adult...

journal_title：Experimental cell research

authors： Shinoura N,Satou R,Yoshida Y,Asai A,Kirino T,Hamada H

更新日期：2000-02-01 00:00:00

abstract：:In preceding studies, the IP3-sensitive Ca2+ store of the hamster insulinoma cell line, HIT, was detected in cell surface protrusions such as microvilli and related membrane structures [Lange, K., and Brandt, U. (1993) FEBS Lett. 320, 183-188; and (1993) FEBS Lett. 325, 205-209]. In this study, these experiments were ...

journal_title：Experimental cell research

authors： Lange J,Schlieps K,Lange K,Brandt U,Knoll-Köhler E

更新日期：1996-11-01 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:The male fetal lung begins to synthesize surfactant later in gestation than the female. This delay appears to be caused by androgens. We hypothesized that male fetal lung differentiation is delayed as a consequence of an extended phase of growth which is elicited by androgens. We observed that in vivo fetal lung prote...

journal_title：Experimental cell research

authors： Nielsen HC,Kirk WO,Sweezey N,Torday JS

更新日期：1990-05-01 00:00:00

abstract：:Alzheimer's disease (AD) is characterized by early degeneration of cholinergic neurons and decreased levels of nerve growth factor (NGF). Thus, increasing the NGF levels by for instance encapsulated cell bio-delivery (ECB) is a potential treatment strategy. The results from our previous first-in-human studies on ECB o...

journal_title：Experimental cell research

authors： Eriksdotter M,Navarro-Oviedo M,Mitra S,Wahlberg L,Linderoth B,Tjernberg LO,Behbahani H

更新日期：2018-10-01 00:00:00

abstract：:In the trabecular meshwork (TM) of the eye, regulation of tissue contractility by the PPRARI sequence within the Heparin II (HepII) domain of fibronectin is believed to control the movement of aqueous humor and dictate the level of intraocular pressure. This study shows that the HepII domain utilizes activated alpha4b...

journal_title：Experimental cell research

authors： Schwinn MK,Gonzalez JM Jr,Gabelt BT,Sheibani N,Kaufman PL,Peters DM

更新日期：2010-05-15 00:00:00

abstract：:Culture and UV (254 nm) irradiation conditions that are suggested as appropriate for a study of the effect of UV on the limited in vitro lifespan of a normal human diploid fibroblast (HDF) strain are first described. An inoculation density at each subcultivation of 1.8 x 10(4) viable cells/cm-2 permits the decline in ...

journal_title：Experimental cell research

authors： Kantor GJ,Mulkie JR,Hull DR

更新日期：1978-05-01 00:00:00

abstract：:Human and rodent cells undergoing apoptosis were observed to express high levels of a novel 45,000 M(r) protein. The protein, which we have termed apoptosis specific protein (ASP), was found in Burkitt lymphoma (BL) cells and in adenovirus-transformed human and rat embryo cells induced into apoptosis by a variety of s...

journal_title：Experimental cell research

authors： Grand RJ,Milner AE,Mustoe T,Johnson GD,Owen D,Grant ML,Gregory CD

更新日期：1995-06-01 00:00:00

abstract：:Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...

journal_title：Experimental cell research

authors： McFarlane C,Hennebry A,Thomas M,Plummer E,Ling N,Sharma M,Kambadur R

更新日期：2008-01-15 00:00:00

abstract：:The expression level of DNA polymerase beta was determined in various mouse tissues. Northern blot hybridization analysis using rat cDNA as a probe revealed that the mRNA of about 1.5 kb for this enzyme is present in all kinds of tissues examined, but its content widely varies among tissues; the most abundant DNA poly...

journal_title：Experimental cell research

authors： Hirose F,Hotta Y,Yamaguchi M,Matsukage A

更新日期：1989-03-01 00:00:00

abstract：:Regions of heterochromatin are often found at the periphery of the mammalian nucleus, juxtaposed to the nuclear lamina. Genes in these regions are likely maintained in a transcriptionally silent state, although other locations at the nuclear periphery associated with nuclear pores are sites of active transcription. As...

journal_title：Experimental cell research

authors： Lee DC,Welton KL,Smith ED,Kennedy BK

更新日期：2009-04-01 00:00:00

abstract：:Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...

journal_title：Experimental cell research

authors： Pujol JP,Penfornis H,Arenzana-Seisdedos F,Bocquet J,Farjanel J,Rattner A,Brisset M,Virelizier JL,Beliard R,Loyau G

更新日期：1985-05-01 00:00:00

abstract：:Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...

journal_title：Experimental cell research

authors： Clark-Knowles KV,Garson K,Jonkers J,Vanderhyden BC

更新日期：2007-01-01 00:00:00