Centrosomal and cytoplasmic Cdc2/cyclin B1 activation precedes nuclear mitotic events.

abstract：:In this study, we demonstrate the protective effects of Cycloartenyl ferulate (CF) against Paraquat (PQ)-induced cytotoxicity and elucidate the underlying molecular mechanisms. The results show that, CF could reverse the PQ-induced growth inhibition and release of lactate dehydrogenase in HK-2 human proximal tubular c...

journal_title：Experimental cell research

authors： Hong GL,Liu JM,Zhao GJ,Wang L,Liang G,Wu B,Li MF,Qiu QM,Lu ZQ

更新日期：2013-11-01 00:00:00

abstract：:The inhibition of apoptosis by Toxoplasma gondii is governed by its modulation of several signaling cascades including the NFkappaappaB and JNK pathways. This is evident in the dysregulation of JNK activation following treatment with UV and TNFalpha, both apoptogenic stimuli. Infection-mediated interference with the J...

journal_title：Experimental cell research

authors： Carmen JC,Southard RC,Sinai AP

更新日期：2008-12-10 00:00:00

abstract：:Many cell types secrete plasma membrane-bound microvesicles, suggested to play an important role in tissue morphogenesis, wound healing, and cancer spreading. However, the mechanisms of their formation have remained largely unknown. It was found that the tips of long microvilli induced in cells by overexpression of hy...

journal_title：Experimental cell research

authors： Rilla K,Pasonen-Seppänen S,Deen AJ,Koistinen VVT,Wojciechowski S,Oikari S,Kärnä R,Bart G,Törrönen K,Tammi RH,Tammi MI

更新日期：2013-08-01 00:00:00

abstract：:We previously showed that clonable skeletal myogenic cells can be derived from the embryonic aorta but become very rare in the more mature and structured fetal aorta. The aim of this study was to investigate whether, during fetal and postnatal development, these myogenic progenitors progressively disappear or may rath...

journal_title：Experimental cell research

authors： Cusella De Angelis MG,Balconi G,Bernasconi S,Zanetta L,Boratto R,Galli D,Dejana E,Cossu G

更新日期：2003-11-01 00:00:00

abstract：:Mitochondrial DNA depleted (ρ(0)) human skin fibroblasts (HSF) with suppressed oxidative phosphorylation were characterized by significant changes in the expression of 2100 nuclear genes, encoding numerous protein classes, in NF-κB and STAT3 signaling pathways, and by decreased activity of mitochondrial death pathway,...

journal_title：Experimental cell research

authors： Ivanov VN,Ghandhi SA,Zhou H,Huang SX,Chai Y,Amundson SA,Hei TK

更新日期：2011-07-01 00:00:00

abstract：:Cystatins constitute a superfamily of cysteine protease inhibitors. A member of the type II secreted cystatin family, cystatin F, has been identified through different gene array experiments to be specifically expressed in hematopoietic cells as well as to be associated with several malignant tumors, suggesting a role...

journal_title：Experimental cell research

authors： Cappello F,Gatti E,Camossetto V,David A,Lelouard H,Pierre P

更新日期：2004-07-15 00:00:00

abstract：:Liver endothelium has been reported to possess membrane receptors for the iron-binding protein transferrin (Tf). Similarly, the core protein of proteoglycans (PG) associated with cell membrane in many cell systems can bind Tf. To find out if membrane-associated proteoglycans can explain Tf-binding ability of liver end...

journal_title：Experimental cell research

authors： Omoto E,Minguell JJ,Tavassoli M

更新日期：1990-03-01 00:00:00

abstract：:We have investigated the gene targeting frequency at the hprt locus in a range of embryonic stem cell lines selected for variations in cell cycle parameters. Our results show that targeting frequency varies with cell line by as much as 12-fold between nonisogenic lines and 3-fold between isogenic lines and that a noni...

journal_title：Experimental cell research

authors： Udy GB,Parkes BD,Wells DN

更新日期：1997-03-15 00:00:00

abstract：:Our results showed that the expression of the src gene in chick embryo fibroblasts (CEF) released the density-dependent inhibition (DDI) of phosphate metabolism (phosphate uptake and phosphorylation of small organic compounds). With increasing cell density, phosphate metabolism decreased by 58% in normal CEF and, in c...

journal_title：Experimental cell research

authors： Jullien M,Harel L,Golde A,Villaudy J,Pugnet P

更新日期：1984-06-01 00:00:00

abstract：:Tunicamycin, an inhibitor of one of the earliest steps in the synthesis of N-linked oligosaccharides, prevents bud formation and growth in Saccharomyces cerevisiae cells that are either growing exponentially or recovering from different cell cycle arrests at start. Analysis of tunicamycin-treated cells by flow microfl...

journal_title：Experimental cell research

authors： Vai M,Popolo L,Alberghina L

更新日期：1987-08-01 00:00:00

abstract：:The retention of mRNAs near the nuclei that synthesize them may be an important feature of the organization of multinucleated skeletal myotubes. Here, we assess the possible role of two factors in this localization. First, we examine the role of mRNA half-life, by studying the distribution of the mRNA for the transfer...

journal_title：Experimental cell research

authors： Ralston E,McLaren RS,Horowitz JA

更新日期：1997-11-01 00:00:00

abstract：:Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root gan...

journal_title：Experimental cell research

authors： Fedorov YV,Prudovsky IA,Yegorov YE,Zelenin AV

更新日期：1993-11-01 00:00:00

abstract：:Satellite cells are a quiescent heterogeneous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-beta (TGF-beta) superfamily members are elevated post-injury and their importance in the r...

journal_title：Experimental cell research

authors： Schabort EJ,van der Merwe M,Loos B,Moore FP,Niesler CU

更新日期：2009-02-01 00:00:00

abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

journal_title：Experimental cell research

authors： Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

更新日期：2008-08-01 00:00:00

abstract：:TGFβ contributes to mesangial cell hypertrophy and matrix protein increase in various kidney diseases including diabetic nephropathy. Deptor is an mTOR-interacting protein and suppresses mTORC1 and mTORC2 activities. We have recently shown that TGFβ-induced inhibition of deptor increases the mTOR activity. The mechani...

journal_title：Experimental cell research

authors： Maity S,Bera A,Ghosh-Choudhury N,Das F,Kasinath BS,Choudhury GG

更新日期：2018-03-01 00:00:00

abstract：:The androgen receptor (AR) plays critical roles in human prostate carcinoma progression and transformation. However, the activation of AR is regulated by co-regulators. MEIS1 protein, the homeodomain transcription factor, exhibited a decreased level in poor-prognosis prostate tumors. In this study, we investigated a p...

journal_title：Experimental cell research

authors： Cui L,Li M,Feng F,Yang Y,Hang X,Cui J,Gao J

更新日期：2014-10-15 00:00:00

abstract：:Reversible methylation of the protein phosphatase 2A catalytic subunit (PP2A(C)(1)) is an important regulatory mechanism playing a crucial role in the selective recruitment of regulatory B subunits. Here, we investigated the subcellular localization of leucine carboxyl methyltransferase (LCMT1) and protein phosphatase...

journal_title：Experimental cell research

authors： Longin S,Zwaenepoel K,Martens E,Louis JV,Rondelez E,Goris J,Janssens V

更新日期：2008-01-01 00:00:00

abstract：:We report that prosaposin binds to U937 and is active as a protective factor on tumor necrosis factor alpha (TNFalpha)-induced cell death. The prosaposin-derived saposin C binds to U937 cells in a concentration-dependent manner, suggesting that prosaposin behaves similarly. Prosaposin binding induces U937 cell death p...

journal_title：Experimental cell research

authors： Misasi R,Garofalo T,Di Marzio L,Mattei V,Gizzi C,Hiraiwa M,Pavan A,Grazia Cifone M,Sorice M

更新日期：2004-08-01 00:00:00

abstract：:Endocytosis is inhibited by overexpression of either dynamin-1 or dynamin-2 mutants because both isoforms form heterotetramers with endogenous dynamin-2 and interfere with its function. By contrast, other phenotypes, which are specifically triggered by overexpression of dynamin-2, but not dynamin-1 are likely to refle...

journal_title：Experimental cell research

authors： Soulet F,Schmid SL,Damke H

更新日期：2006-11-01 00:00:00

abstract：:Mutations in the tumor suppressor gene p53 were found in more than 90% of all human squamous cell carcinomas (SCC). To study the function of p53 in a keratinocyte background, a tetracycline-controlled p53 transgene was introduced into a human SCC cell line (SCC15), lacking endogenous p53. Conditional expression of wil...

journal_title：Experimental cell research

authors： Niemantsverdriet M,Jongmans W,Backendorf C

更新日期：2005-10-15 00:00:00

abstract：:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

journal_title：Experimental cell research

authors： Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

更新日期：2012-03-10 00:00:00

abstract：:The mechanisms of replicative senescence by telomere shortening are not fully understood. The Indian muntjac has the fewest chromosomes of all mammals, greatly simplifying the analysis of each telomere over time. In this study, telomere shortening was observed throughout the life span of cultured normal muntjac cells ...

journal_title：Experimental cell research

authors： Zou Y,Yi X,Wright WE,Shay JW

更新日期：2002-11-15 00:00:00

abstract：:Butyrate and other short-chain fatty acids (SCFA), produced by colonic bacterial flora, affect numerous epithelial cell functions. To better understand how SCFA regulate ion transport, we investigated the effects of 4-phenylbutyrate (4-PBA) on Na(+) absorption in T84 cells. Under standard cell culture conditions, the ...

journal_title：Experimental cell research

authors： Iordache C,Duszyk M

更新日期：2007-01-15 00:00:00

abstract：:Skeletal muscle differentiation is strongly inhibited by transforming growth factor type beta (TGF-beta), although muscle formation as well as regeneration normally occurs in an environment rich in this growth factor. In this study, we evaluated the role of intracellular regulatory Smads proteins as well as TGF-beta-r...

journal_title：Experimental cell research

authors： Droguett R,Cabello-Verrugio C,Santander C,Brandan E

更新日期：2010-09-10 00:00:00

abstract：:Celiac disease (CD) is a multisystemic autoimmune inflammation of the intestinal tract induced by wheat gluten and related cereals in HLA-DQ2/8 positive individuals. An essential role in the pathogenesis of CD is played by a fraction of the peptic-tryptic digest of gluten, Frazer's Fraction (FF). Here, we investigate ...

journal_title：Experimental cell research

authors： Reinke Y,Zimmer KP,Naim HY

更新日期：2009-11-15 00:00:00

abstract：:Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I...

journal_title：Experimental cell research

authors： Freise C,Bobb V,Querfeld U

更新日期：2017-09-15 00:00:00

abstract：:Pevonedistat is a potent, selective, first-in-class NEDD8 activating enzyme inhibitor. It is now under multiple clinical trials that investigate its anticancer effect against solid tumors and leukemia. ATP-binding cassette (ABC) transporters are membrane proteins that are involved in mediating multidrug resistance (MD...

journal_title：Experimental cell research

authors： Wei LY,Wu ZX,Yang Y,Zhao M,Ma XY,Li JS,Yang DH,Chen ZS,Fan YF

更新日期：2020-03-15 00:00:00

abstract：:Megakaryocytes (Mks) are unique cells in the human body in that they carry a single and polyploid nucleus. It is therefore of interest to understand their nuclear ultrastructure. PML oncogenic domains (PODs) were described in several types of eukaryotic cells using human autoantibodies which recognize nuclear antigens...

journal_title：Experimental cell research

authors： Drouin A,Schmitt A,Massé JM,Cieutat AM,Fichelson S,Cramer EM

更新日期：2001-12-10 00:00:00

abstract：:We have used biotinylated rDNA probes to localize by in situ hybridization the extrachromosomal genes for ribosomal RNA in the slime mold Physarum polycephalum. We established conditions that allow for highly specific hybridization at the ultrastructural level and determined that the 60-kb palindromic rDNA molecules a...

journal_title：Experimental cell research

authors： Puvion-Dutilleul F,Pierron G

更新日期：1992-12-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress the expression of their target genes post-transcriptionally. MiRNAs participate in the regulation of a variety of biological processes, including development and diseases. However, the functional role and molecular mechanism by which miRNAs regulate ...

journal_title：Experimental cell research

authors： Wang L,Chen X,Zheng Y,Li F,Lu Z,Chen C,Liu J,Wang Y,Peng Y,Shen Z,Gao J,Zhu M,Chen H

更新日期：2012-11-01 00:00:00