Spatial control of protein phosphatase 2A (de)methylation.

abstract：:Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleo...

journal_title：Experimental cell research

authors： Schwab MS,Gossweiler U,Dreyer C

更新日期：1998-03-15 00:00:00

abstract：:Transforming growth factor beta regulates many biological processes including cell motility and invasion. Podosomes are specialized F-actin rich structures found in normal cells, such as osteoclasts and macrophages. Tumor cells often form related structures called invadopodia that are thought to promote invasion and m...

journal_title：Experimental cell research

authors： Mandal S,Johnson KR,Wheelock MJ

更新日期：2008-11-15 00:00:00

abstract：:Hypoxic blood-brain barrier (BBB) dysfunction is a common feature of CNS diseases however mechanisms underlying barrier disturbance are still largely unknown. This study investigated the role of transforming growth factor β (TGFβ), a cytokine known to induce expression of the proprotein convertase Furin, in hypoxia-me...

journal_title：Experimental cell research

authors： Baumann J,Huang SF,Gassmann M,Tsao CC,Ogunshola OO

更新日期：2019-10-15 00:00:00

abstract：:Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...

journal_title：Experimental cell research

authors： List HJ,Smith CL,Martinez E,Harris VK,Danielsen M,Riegel AT

更新日期：2000-10-10 00:00:00

abstract：:Non-small cell lung carcinoma (NSCLC) is characterized by resistance to drug-induced apoptosis, which might explain the survival of lung cancer cells following treatment. Recently we have shown that the broad-range kinase inhibitor staurosporine (STS) reactivates the apoptotic machinery in U1810 NSCLC cells [Joseph et...

journal_title：Experimental cell research

authors： Hemström TH,Joseph B,Schulte G,Lewensohn R,Zhivotovsky B

更新日期：2005-04-15 00:00:00

abstract：:U73122, a phospholipase C inhibitor, dose dependently blocks the cGMP-induced Ca2+ release in sea urchin eggs and homogenates. U73122 inhibition was prevented by cotreatment with dithiothreitol (DTT), but DTT is ineffective when eggs or homogenates were pretreated with U73122. U73122 action is different from the other...

journal_title：Experimental cell research

authors： Lee SJ,Madden PJ,Shen SS

更新日期：1998-07-10 00:00:00

abstract：:Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts a...

journal_title：Experimental cell research

authors： Li J,Li H,Tian Y,Yang Y,Chen G,Guo W,Tian W

更新日期：2016-07-01 00:00:00

abstract：:In this communication, we have studied a 72,000 mol w (p72) host protein which reacts with a mouse monoclonal antibody (PAb6) directed against antigenic determinants on the Simian virus 40 (SV40) large T antigen protein that map 5' of 0.42 map units on the viral genome. The p72 protein is an abundant basic (pI greater...

journal_title：Experimental cell research

authors： Ullrich SJ,Appella E,Mercer WE

更新日期：1988-10-01 00:00:00

abstract：:Germ cells were isolated from mouse fetal gonads 11 1/2-16 1/2 days post coitum (dpc), and exposed to various methods of in vitro culture. From 13 1/2 dpc onwards, both male and female germ cells survived well at 37 degrees C for several days. During the culture period the proportion of female germ cells in meiosis in...

journal_title：Experimental cell research

authors： De Felici M,McLaren A

更新日期：1983-04-01 00:00:00

abstract：:Tumor angiogenesis and immune response have in common to be cell recognition mechanisms, which are based on specific adhesion molecules and dependent on nitric oxide (NO(•)). The aim of the present study is to deepen the mechanisms of angiogenesis and inflammation regulation by NO(•) to find out the molecular regulati...

journal_title：Experimental cell research

authors： Carreau A,Kieda C,Grillon C

更新日期：2011-01-01 00:00:00

abstract：:We have previously shown that following psoralen photoactivation (PUVA treatment) human dermal fibroblasts undergo long-term growth arrest as well as morphological and functional changes reminiscent of cellular senescence [ 1 ]. In the absence of molecular data on what constitutes normal senescence, it has been diffic...

journal_title：Experimental cell research

authors： Ma W,Wlaschek M,Brenneisen P,Schneider LA,Hommel C,Hellweg C,Sauer H,Wartenberg M,Herrmann G,Meewes C,Boukamp P,Scharffetter-Kochanek K

更新日期：2002-04-01 00:00:00

abstract：:The migration and proliferation of vascular smooth muscle cells (vSMCs) are critical events in neointima formation during atherosclerosis and restenosis. The transcription factor nuclear factor of activated T-cells-isoform c1 (NFATc1) is regulated by atherogenic cytokines, and has been implicated in the migratory and ...

journal_title：Experimental cell research

authors： Chow W,Hou G,Bendeck MP

更新日期：2008-10-01 00:00:00

abstract：:Previously we showed that the potocytosis of 5-methyltetrahydrofolate is dependent on the acidification of plasmalemmal vesicles created each time a caveolae, closes off from the cell surface. We now report that bafilomycin A1, which is a specific inhibitor of the V-type proton ATPase, inhibits 5-methyltetrahydrofolat...

journal_title：Experimental cell research

authors： Mineo C,Anderson RG

更新日期：1996-05-01 00:00:00

abstract：:Autosomal dominantly inherited missense mutations in lamins A and C cause familial partial lipodystrophy of the Dunnigan-type (FPLD), and myopathies including Emery-Dreifuss muscular dystrophy (EDMD). While mutations responsible for FPLD are restricted to the carboxyl-terminal tails, those responsible for EDMD are spr...

journal_title：Experimental cell research

authors： Favreau C,Dubosclard E,Ostlund C,Vigouroux C,Capeau J,Wehnert M,Higuet D,Worman HJ,Courvalin JC,Buendia B

更新日期：2003-01-01 00:00:00

abstract：:Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unc...

journal_title：Experimental cell research

authors： Bhoonderowa L,Hameurlaine F,Arbabian A,Faqir F,Amblard F,Coscoy S

更新日期：2016-10-15 00:00:00

abstract：:The adaptor protein Shb has previously been shown to regulate apoptosis in response to cytokines and inhibitors of angiogenesis although the mechanisms governing these effects have remained obscure. We currently demonstrate interactions between Shb and c-Abl and that Shb regulates c-Abl kinase activity. The data sugge...

journal_title：Experimental cell research

authors： Hägerkvist R,Mokhtari D,Lindholm C,Farnebo F,Mostoslavsky G,Mulligan RC,Welsh N,Welsh M

更新日期：2007-01-15 00:00:00

abstract：:Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...

journal_title：Experimental cell research

authors： Corbett A,Ferrigno P,Henry M,Kahana J,Koepp D,Lee M,Nguyen L,Schlenstedt G,Seedorf M,Shen E,Taura T,Wong D,Silver P

更新日期：1996-12-15 00:00:00

abstract：:The process of tumor cell invasion of the basement membrane is proposed to consist of three steps: attachment, local proteolysis and migration. 12-(S)-HETE, a 12-lipoxygenase metabolite of arachidonic acid, upregulates surface expression of integrin cytoadhesins and an autocrine motility factor receptor, suggesting th...

journal_title：Experimental cell research

authors： Honn KV,Timár J,Rozhin J,Bazaz R,Sameni M,Ziegler G,Sloane BF

更新日期：1994-09-01 00:00:00

abstract：:A monoclonal antibody raised against microtubule-associated protein-1 (MAP-1) binds to nuclei of normal human fibroblasts, its binding site being detected as intranuclear immunofluorescent dots. The percentage of cells showing these dots increased with insulin, hydrocortisone, epidermal growth factor (EGF), and transf...

journal_title：Experimental cell research

authors： Ohno T,Ohkawa A,Sato C

更新日期：1985-06-01 00:00:00

abstract：:Dominant mutations in presenilin1 (PS1) and presenilin2 (PS2) are a major cause of early-onset Alzheimer's disease. In this report we analyze the expression of the zebrafish presenilin1 (Psen1) and presenilin2 (Psen2) proteins during embryogenesis. We demonstrate that Psen1 and Psen2 holoproteins are relatively abunda...

journal_title：Experimental cell research

authors： Nornes S,Groth C,Camp E,Ey P,Lardelli M

更新日期：2003-09-10 00:00:00

abstract：:32D cells grown for 1 year in interleukin-3 (IL-3) and granulocyte colony-stimulating factor (G-CSF) generated the 32D Ro cell line which retained the parental mast cell phenotype but lost ability to generate erythroid cells in response to erythropoietin (EPO). In order to clarify the mechanisms underlying such restri...

journal_title：Experimental cell research

authors： Baiocchi M,Di Rico C,Di Pietro R,Di Baldassarre A,Migliaccio AR

更新日期：2003-05-01 00:00:00

abstract：:Identification of transcription factors expressed by differentiated cells is informative not only of tissue-specific pathways, but to help identify master regulators for cellular reprogramming. If applied, such an approach could generate healthy autologous tissue-specific cells for clinical use where cells from the ho...

journal_title：Experimental cell research

authors： Hustler A,Eardley I,Hinley J,Pearson J,Wezel F,Radvanyi F,Baker SC,Southgate J

更新日期：2018-08-15 00:00:00

abstract：:The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...

journal_title：Experimental cell research

authors： Broadley C,Dawidowicz E,Chong PL,Hoover R

更新日期：1991-03-01 00:00:00

abstract：:The aim of the present study was to determine whether mitochondrial activity could regulate myoblast proliferation. We demonstrate that an increase in mitochondrial activity of L6E9 myoblasts can be easily obtained by simply raising extracellular pyruvate concentration in the culture dish. Under this condition, L6E9 m...

journal_title：Experimental cell research

authors： Duguez S,Sabido O,Freyssenet D

更新日期：2004-09-10 00:00:00

abstract：:The establishment of cell lines allows reproductible in vitro studies that would be far more difficult to perform using primary cells that rapidly undergo phenotypical alterations in culture. The purpose of this work was to establish an endothelial cell line appropriate for in vitro study of endothelial cell activatio...

journal_title：Experimental cell research

authors： Malassagne B,Taboit F,Conti F,Batteux F,Atia N,Chéreau C,Conjeaud H,Théron MC,Attal J,Braet F,Houdebine LM,Calmus Y,Houssin D,Weill B

更新日期：1998-01-10 00:00:00

abstract：:Liver cells of new-born rats, which were found to be able to form spheroidal aggregates when cultured on a nonadherent plastic substratum, were studied under various conditions of culture, mainly by adding different nutrients and growth factors to the culture medium. Analysis of hepatocyte-specific functions was carri...

journal_title：Experimental cell research

authors： Tong JZ,Bernard O,Alvarez F

更新日期：1990-07-01 00:00:00

abstract：:The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...

journal_title：Experimental cell research

authors： Sakkas D,Mariethoz E,St John JC

更新日期：1999-09-15 00:00:00

abstract：:The highly purified, luminal domain of rat lung endothelial plasma membranes was used as an immunogen to obtain monoclonal antibodies to the endothelial cell surface. The procedure was highly efficient, yielding antibodies which recognize three seemingly novel endothelial integral membrane glycoproteins of molecular w...

journal_title：Experimental cell research

authors： Ghitescu LD,Crine P,Jacobson BS

更新日期：1997-04-10 00:00:00

abstract：:The dynamics of lamin disassembly and reassembly during sea urchin male pronuclear development in vitro was investigated. Using five anti-lamin antibodies, we monitored by immunofluorescence and immunoblotting the changes in lamins during sperm chromatin decondensation, nuclear envelope (NE) formation, and male pronuc...

journal_title：Experimental cell research

authors： Collas P,Pinto-Correia C,Poccia DL

更新日期：1995-08-01 00:00:00

abstract：:Liver membrane glycoproteins with affinity for immobilized collagen type I were subjected to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution of the separated proteins. Electroeluted glycoproteins with ability to neutralize the inhibitory effect of anti-CollCA...

journal_title：Experimental cell research

authors： Gullberg D,Terracio L,Rubin K

更新日期：1988-04-01 00:00:00