Abstract:
:A monoclonal antibody raised against microtubule-associated protein-1 (MAP-1) binds to nuclei of normal human fibroblasts, its binding site being detected as intranuclear immunofluorescent dots. The percentage of cells showing these dots increased with insulin, hydrocortisone, epidermal growth factor (EGF), and transferrin in serum-free medium, their effects decreasing in this order. The appearance of dots induced by serum or insulin plus hydrocortisone was dependent on the Ca2+ concentration of the medium. The Ca2+ ionophore A23187 increased the rate of appearance of cells containing dots. Conversely, the calmodulin inhibitor W-7 blocked their appearance and decreased the number of cells containing dots. The dose-response curves of W-7 for inhibition of the appearance of dots and of DNA synthesis were essentially identical. These results suggest that the appearance of intranuclear MAP-1 antigen is controlled mainly by insulin, Ca2+ and calmodulin and is associated with DNA replication.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ohno T,Ohkawa A,Sato Cdoi
10.1016/0014-4827(85)90480-xsubject
Has Abstractpub_date
1985-06-01 00:00:00pages
558-62issue
2eissn
0014-4827issn
1090-2422journal_volume
158pub_type
杂志文章abstract::Transcription factors Mitf and NFATc1 share many downstream targets that are critical for osteoclastogenesis. Since RANKL signals induce/activate both NFATc1 and Mitf isoform-E (Mitf-E), a tissue-restricted Mitf isoform in osteoclasts, it is plausible that the two factors work together to promote osteoclastogenesis. A...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.08.018
更新日期:2014-10-15 00:00:00
abstract::Most coronary smooth muscle cells(CoSMCs) are differentiated from epicardial progenitor cells(EPCs), but the specific mechanism is not fully investigated. Previous studies have shown that autophagy plays an important role for smooth muscle cells(SMCs) differentiation, yet whether autophagy is involved in the different...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.12.025
更新日期:2019-02-15 00:00:00
abstract::The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00386-0
更新日期:2003-11-15 00:00:00
abstract::In culture, nontransformed human diploid fibroblasts divide a limited number of times, resulting in a nonproliferating senescent cell culture which exhibits an altered pattern of gene expression. Previously we reported that an early event in the process of replicative senescence was an increase in the synthesis of two...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0326
更新日期:1996-11-01 00:00:00
abstract::Prenucleolar bodies (PNBs) are transitory structures which serve as building blocks for nucleoli at the transition mitosis/interphase. The assembly of PNBs and their pathway are not clearly understood. To better understand these events, the behavior of the PNB-containing PM-Scl 100 protein was compared with that of ot...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4578
更新日期:1999-09-15 00:00:00
abstract::The centromere is a specialized region of the eukaryotic chromosome that is responsible for directing chromosome movements in mitosis and for coordinating the progression of mitotic events at the crucial transition between metaphase and anaphase. In this review, we will focus on recent advances in the understanding of...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1006/excr.1998.4278
更新日期:1999-02-01 00:00:00
abstract::We have compared the in vitro replicative life span and characteristics of immortalization of skin fibroblast cultures derived from ad libitum-fed and caloric-restricted Fischer 344 rats of 6, 24, and 29 months of age. Cells from all 6-, 24-, and 29-month-old animals showed a gradual decline in proliferative potential...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90343-7
更新日期:1992-07-01 00:00:00
abstract::We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. Th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1098
更新日期:1993-04-01 00:00:00
abstract::Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90554-8
更新日期:1991-03-01 00:00:00
abstract::Alexander disease (AxD) is a rare neurodegenerative disorder characterized by large cytoplasmic aggregates in astrocytes and myelin abnormalities and caused by dominant mutations in the gene encoding glial fibrillary acidic protein (GFAP), the main intermediate filament protein in astrocytes. We tested the effects of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.04.035
更新日期:2007-08-01 00:00:00
abstract::The gene cluster for human U2 RNA has been mapped to chromosome 17q21 by in situ hybridization and hybridization analysis of DNA from mouse/human somatic cell hybrids. ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(85)80020-3
更新日期:1985-08-01 00:00:00
abstract::The glomerular capillary endothelium is highly specialized to support the selective filtration of massive volumes of plasma. Filtration is driven by Starling forces acting across the glomerular capillary wall, and depends on its large surface area and extremely high water permeability. Glomerular endothelial cells are...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2012.02.032
更新日期:2012-05-15 00:00:00
abstract::The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90548-9
更新日期:1991-03-01 00:00:00
abstract::The involvement of p53 as a determinant of chemosensitivity or radiosensitivity is not well understood and is complicated by numerous contradictory reports. Here we have addressed this issue using a series of isogenic clones derived from two neuroblastoma cell lines that express wild-type p53 genes, Nub7 and IMR32. Tw...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5493
更新日期:2002-04-15 00:00:00
abstract::Stem cells with the ability to differentiate in specialized cell types can be extracted from a wide array of adult tissues including skeletal muscle. Here we have analyzed a population of cells isolated from skeletal muscle on the basis of their poor adherence on uncoated or collagen-coated dishes that show multi-line...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.01.009
更新日期:2008-04-01 00:00:00
abstract::Distal-less homeobox 5 (Dlx5) is a pro-osteogenic but anti-adipogenic transcription factor that regulates lineage commitment in mesenchymal stem cells. Although the expression of Dlx5 is known to be decreased by adipogenic stimuli, the mechanism of Dlx5 down-regulation has not yet been clarified. MicroRNAs (miRNAs) ar...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.04.020
更新日期:2013-08-15 00:00:00
abstract::Primary cultures of human keratinocytes form a multilayered tissue. By incubating the tissue cultures in Ca2(+)-free medium the differentiated cell layers can be stripped off leaving a basal cell monolayer. We have developed a method for high-frequency transfection of these epidermal basal cells with genes inserted in...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90231-x
更新日期:1990-08-01 00:00:00
abstract::CEACAM1-4S (carcinoembryonic antigen-related cell adhesion molecule 1) is a type I membrane protein with a short (12-amino acid) cytoplasmic tail. Wild type CEACAM1-4S-transfected MCF7 cells form glands with lumena when grown in 3D culture, while null mutations of two putative phosphorylation sites (T457A and S459A) i...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.11.001
更新日期:2010-02-15 00:00:00
abstract::The protein encoded by C-terminal alternatively spliced p53 mRNA (p53as) has been shown previously to occur naturally in mouse cells and to bind sequence-specifically to DNA more efficiently than p53 (p53r, regular form). In the current study, p53as and p53r proteins ectopically expressed in p53-deficient cells each t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5596
更新日期:2002-10-01 00:00:00
abstract::Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts a...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2015.12.011
更新日期:2016-07-01 00:00:00
abstract::Differentiation and apoptosis are precisely regulated events in early embryogenesis. Retinoic acid-induced differentiation in the embryonal carcinoma (EC) cell line NCR-G3 triggers concurrent induction of apoptosis. Using this system, which serves as a model of early embryogenesis, the expression of various bcl-2-rela...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5203
更新日期:2001-05-15 00:00:00
abstract::The puromycin analog N6,N6-dimethyladenine (6-dimethylaminopurine or 6-DMAP) was found to inhibit meiosis reinitiation in starfish oocytes stimulated by the natural hormone 1-methyladenine. Increasing concentrations of this agent delayed and eventually blocked germinal vesicle breakdown. They were found to be effectiv...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90121-8
更新日期:1988-05-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common cancers diagnosed worldwide. However, the mechanism underlying HCC pathogenesis remains unknown. In the present study, TRIM24 was found increased in human HCC clinical samples and positively correlated with HCC tumor grade. Furthermore, TRIM24 knockdown inhibits...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.04.006
更新日期:2018-06-15 00:00:00
abstract::Gallbladder cancer (GBC) is an aggressive malignancy of the bile duct, which is associated with a low (5-year) survival and poor prognosis. The transcription factor HIF-1α is implicated in the angiogenesis, cell survival, epithelial mesenchymal transition (EMT) and invasiveness of GBC. In this study, we have investiga...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.11.021
更新日期:2015-03-15 00:00:00
abstract::In low serum (0.2%) medium, ascorbate stimulates primary avian tendon cells to increase procollagen synthesis from 12 to 50% of total protein synthesis. This is reversibly blocked by an increase of serum levels from 0.2 to 3%. Ascorbate in low serum medium has been shown previously to stimulate the procollagen pathway...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90330-8
更新日期:1988-06-01 00:00:00
abstract::Several cell division cycle (cdc) mutants of Saccharomyces cerevisiae (cdc28, cdc35, cdc19, cdc21, and cdc17) at the restrictive temperature (37 degrees C) in the presence of 1% glucose and defined medium divert most of the carbon (approximately 50%) to ethanol production with low biomass growth yields (Yglc) that cor...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1062
更新日期:1995-03-01 00:00:00
abstract::The biosynthesis of type XI and type II collagens was examined using a stable rat chondrocyte cell line established by W. E. Horton et al. (1988, Exp. Cell Res. 178, 457-468.). These cells (IRC; immortalized rat chondrocytes) were created by transformation with a murine retrovirus carrying the v-myc and v-raf oncogene...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1169
更新日期:1994-07-01 00:00:00
abstract::The histone 2A variant macroH2A1.2 is expressed in female and male mammals and is implicated in X-chromosome inactivation and autosomal gene silencing. In undifferentiated and early differentiating murine embryonic stem (ES) cells a cytosolic pool of macroH2A1.2 has recently been reported and found to be associated wi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5277
更新日期:2001-08-15 00:00:00
abstract::Wnt1 and -5a have been shown to modulate the proliferation and differentiation of midbrain dopaminergic (DA) neurons. However, it is not known whether other Wnts or which Frizzled (Fz) receptors are expressed in the developing midbrain. We found that 13 out of 19 Wnts, all 10 Fzs, and several intracellular Wnt signali...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.01.032
更新日期:2006-05-15 00:00:00
abstract::Insulin-like growth factor I (IGF-I) promotes the motility of different cell types. We investigated the role of IGF-I receptor (IGF-IR) signaling in locomotion of MCF-7 breast cancer epithelial cells overexpressing the wild-type IGF-IR (MCF-7/IGF-IR). Stimulation of MCF-7/IGF-IR cells with 50 ng/ml IGF-I induced disru...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4566
更新日期:1999-08-25 00:00:00