TGF-beta induces formation of F-actin cores and matrix degradation in human breast cancer cells via distinct signaling pathways.

abstract：:Nuclear pore complexes (NPCs) provide the only sites for macromolecular transport between nucleus and cytoplasm. The nucleoporin p62, a component of higher eukaryotic NPCs, is located at the central gated channel and involved in nuclear trafficking of various cargos. p62 is organized into an N-terminal segment that co...

journal_title：Experimental cell research

authors： Stochaj U,Bański P,Kodiha M,Matusiewicz N

更新日期：2006-08-01 00:00:00

abstract：:In culture, nontransformed human diploid fibroblasts divide a limited number of times, resulting in a nonproliferating senescent cell culture which exhibits an altered pattern of gene expression. Previously we reported that an early event in the process of replicative senescence was an increase in the synthesis of two...

journal_title：Experimental cell research

authors： Zeng G,McCue HM,Mastrangelo L,Millis AJ

更新日期：1996-11-01 00:00:00

abstract：:The movement of a cell through the sequential phases of apoptosis is accompanied by a progressive decrease in cell size with loss in protein mass. In lymphocytes from Hiv-infected persons, protein loss during apoptosis is due to increased protein degradation rather than decreased synthesis. To identify and characteriz...

journal_title：Experimental cell research

authors： Piedimonte G,Crinelli R,Della Salda L,Corsi D,Pennisi MG,Kramer L,Casabianca A,Sarli G,Bendinelli M,Marcato PS,Magnani M

更新日期：1999-05-01 00:00:00

abstract：:Specific cholecystokinin (CCK) receptor and gastrin receptor antagonists were used to assess what role, if any, these receptors have in autocrine cell growth. Although the cholecystokinin receptor antagonist, L-364,718, inhibited cell proliferation in a broad spectrum of cell lines, the gastrin antagonist, L-365,260, ...

journal_title：Experimental cell research

authors： Thumwood CM,Hong J,Baldwin GS

更新日期：1991-01-01 00:00:00

abstract：:Coiled bodies (CBs) are structural constituents observed in nuclei of most eukaryotic cells. They usually occur in the nucleoplasm as well as in contact with the nucleolar surface. In this work we studied the hepatocyte nuclei of hibernating dormice in order to investigate possible modifications of CBs along the seaso...

journal_title：Experimental cell research

authors： Malatesta M,Zancanaro C,Martin TE,Chan EK,Amalric F,Lührmann R,Vogel P,Fakan S

更新日期：1994-04-01 00:00:00

abstract：:Hematopoiesis is the hierarchical process in which all lineages of blood cells are produced by self-renewing hematopoietic stem cells (HSCs) in the bone marrow (BM). While the regulatory factors that maintain proper HSC function and lineage output under normal conditions are well understood, significantly less is know...

journal_title：Experimental cell research

authors： Mirantes C,Passegué E,Pietras EM

更新日期：2014-12-10 00:00:00

abstract：:Within tumors there appears to be an intricate balance between hyaluronan (HA) synthesis and degradation where the invading edges display increased HA metabolism. The metabolism of HA has not been characterized in breast cancer cell lines; therefore, this study quantitatively identifies and characterizes the enzymes r...

journal_title：Experimental cell research

authors： Udabage L,Brownlee GR,Nilsson SK,Brown TJ

更新日期：2005-10-15 00:00:00

abstract：:In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...

journal_title：Experimental cell research

authors： Geiger D,Carpentier JL,Gorden P,Orci L

更新日期：1989-11-01 00:00:00

abstract：:A method for coating liposomes with transferrin is described. Such liposomes have been used for carrying exogenous DNA to rabbit bone marrow (BM) precursor cells, in vivo. A large amount of the DNA contained in the liposomes was delivered specifically into the erythroblasts of the test animals. ...

journal_title：Experimental cell research

authors： Stavridis JC,Deliconstantinos G,Psallidopoulos MC,Armenakas NA,Hadjiminas DJ,Hadjiminas J

更新日期：1986-06-01 00:00:00

abstract：:Abnormal aspartyl residue formation such as L-isoaspartates occurs frequently during aging in long-lived proteins, resulting in the alteration of their structures and biological functions. In this study, we investigated the alteration of aspartyl residues in extracellular matrix (ECM) proteins, type-I collagen and fib...

journal_title：Experimental cell research

authors： Lanthier J,Desrosiers RR

更新日期：2004-02-01 00:00:00

abstract：:The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

journal_title：Experimental cell research

authors： Lang C,Wang J,Chen L

更新日期：2017-11-15 00:00:00

abstract：:Spindle assembly is essential for the equal distribution of genetic material to the daughter cells during mitosis. The process of spindle assembly is complicated and involves multiple levels of molecular regulation. It is generally accepted that mitotic spindles are emanated from the centrosomes and are assembled in t...

journal_title：Experimental cell research

authors： Yang CP,Fan SS

更新日期：2008-10-15 00:00:00

abstract：:Elastin, a major constituent of the media, may play an important role in the attachment, migration, and phenotypic properties of vascular smooth muscle cells (SMC). We examined binding proteins and binding sites on elastin in rabbit arterial SMC in primary culture. Freshly isolated cells on alpha-elastin underwent phe...

journal_title：Experimental cell research

authors： Yamamoto K,Aoyagi M,Yamamoto M

更新日期：1995-05-01 00:00:00

abstract：:Normal and transformed human cells when stained for ezrin, an F-actin-binding ERM (ezrin/radixin/moesin) family protein, revealed a faint and intense immunofluorescence, respectively. Surprisingly, nuclear staining that was assigned to the nucleolus by confocal laser and immunoelectron microscopy was detected in both ...

journal_title：Experimental cell research

authors： Kaul SC,Kawai R,Nomura H,Mitsui Y,Reddel RR,Wadhwa R

更新日期：1999-07-10 00:00:00

abstract：:Neurons require adhesive scaffolds for their growth and differentiation. Laminins are a major cell adhesive component of basement membranes and have various biological activities in the peripheral and central nervous systems. Here, we evaluated the biological activities of 5 peptides derived from laminin-111 as a scaf...

journal_title：Experimental cell research

authors： Hayashi H,Horinokita I,Yamada Y,Hamada K,Takagi N,Nomizu M

更新日期：2020-12-22 00:00:00

abstract：:This review article describes the pathways and mechanisms of endocytosis and post-endocytic sorting of the EGF receptor (EGFR/ErbB1) and other members of the ErbB family. Growth factor binding to EGFR accelerates its internalization through clathrin-coated pits which is followed by the efficient lysosomal targeting of...

journal_title：Experimental cell research

authors： Sorkin A,Goh LK

更新日期：2009-02-15 00:00:00

abstract：:Murine embryonal carcinoma F9 cells can be induced to differentiate by 2-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC). The differentiated phenotype is similar to that of retinoic acid (RA)-treated F9 cells. In contrast to F9 cells the differentiated cells secrete plasminog...

journal_title：Experimental cell research

authors： Jetten AM,Shirley JE

更新日期：1985-01-01 00:00:00

abstract：:Calcium-tolerant myocytes were isolated from adult rat hearts by collagenase perfusion and plated on various substrates in serum-free medium and their adhesion to various extracellular matrix (ECM) components was determined. The myocytes attached readily to dishes coated with collagen type IV (C-IV), laminin (LN), and...

journal_title：Experimental cell research

authors： Lundgren E,Terracio L,Mårdh S,Borg TK

更新日期：1985-06-01 00:00:00

abstract：:Prior incubation of confluent, quiescent cultures of Swiss 3T3 cells with insulin leads to a selective loss of mitogenic stimulation on re-addition of the combination of vasopressin and insulin in serum-free medium. The desensitization is specific for the action of vasopressin as insulin is fully active in the refract...

journal_title：Experimental cell research

authors： Collins MK,Rozengurt E

更新日期：1984-04-01 00:00:00

abstract：:Recruitment of mesenchymal stem cells (MSC) to tissue damages is a promising approach for in situ tissue regeneration. The physiological mechanisms and regulatory processes of MSC trafficking to injured tissue remain poorly understood. However, the pivotal role of chemokines in MSC recruitment has already been shown. ...

journal_title：Experimental cell research

authors： Binger T,Stich S,Andreas K,Kaps C,Sezer O,Notter M,Sittinger M,Ringe J

更新日期：2009-05-01 00:00:00

abstract：:Xenopus early embryos are transcriptionally quiescent until the midblastula transition (MBT). We have examined the question of whether the absence of rRNA synthesis is related to a deficiency in the RNA polymerase I (pol I) transcription machinery. Previously we have demonstrated that the maternally provided pol I tra...

journal_title：Experimental cell research

authors： Bell P,Scheer U

更新日期：1999-04-10 00:00:00

abstract：:We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...

journal_title：Experimental cell research

authors： Ozeki N,Mogi M,Hase N,Hiyama T,Yamaguchi H,Kawai R,Matsumoto T,Nakata K

更新日期：2016-09-10 00:00:00

abstract：:Among the cellular events that are associated with the process of endochondral ossification is an incremental increase in chondrocyte basal intracellular free Ca(2+) concentration ([Ca(2+)](i)) from 50 to 100 nM. To determine if this rise in [Ca(2+)](i) functionally participates in the maturational process of growth p...

journal_title：Experimental cell research

authors： Zuscik MJ,D'Souza M,Ionescu AM,Gunter KK,Gunter TE,O'Keefe RJ,Schwarz EM,Puzas JE,Rosier RN

更新日期：2002-06-10 00:00:00

abstract：:Inflammatory cells such as macrophages can play a pro-tumorigenic role in the tumor stroma. Tumor-associated macrophages (TAMs) generally display an M2 phenotype with tumor-promoting activity; however, the mechanisms regulating the TAM phenotype remain unclear. Complement 5a (C5a) is a cytokine-like polypeptide that i...

journal_title：Experimental cell research

authors： Piao C,Zhang WM,Li TT,Zhang CC,Qiu S,Liu Y,Liu S,Jin M,Jia LX,Song WC,Du J

更新日期：2018-05-15 00:00:00

abstract：:The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...

journal_title：Experimental cell research

authors： Lolkema MP,Mehra N,Jorna AS,van Beest M,Giles RH,Voest EE

更新日期：2004-12-10 00:00:00

abstract：:Interleukin-1 (IL-1) is one of the most potent bone-resorbing factors involved in bone loss associated with inflammation. We previously reported that IL-1 prolonged the survival of multinucleated osteoclast-like cells (OCLs) formed in cocultures of murine osteoblasts/stromal cells and bone marrow cells via the prevent...

journal_title：Experimental cell research

authors： Jimi E,Nakamura I,Duong LT,Ikebe T,Takahashi N,Rodan GA,Suda T

更新日期：1999-02-25 00:00:00

abstract：:An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium a...

journal_title：Experimental cell research

authors： Akatov VS,Lezhnev EI,Vexler AM,Kublik LN

更新日期：1985-10-01 00:00:00

abstract：:Identification of new drugs and predicting drug response are major challenges in oncology, especially for brain tumors, because total surgical resection is difficult and radiation therapy or chemotherapy is often ineffective. With the aim of developing a culture system close to in vivo conditions for testing new drugs...

journal_title：Experimental cell research

authors： Jiguet Jiglaire C,Baeza-Kallee N,Denicolaï E,Barets D,Metellus P,Padovani L,Chinot O,Figarella-Branger D,Fernandez C

更新日期：2014-02-15 00:00:00

abstract：:With multiple divisions in culture, normal diploid cells suffer a loss of growth potential that leads to replicative senescence and a finite replicative capacity. Using quantitative RT-PCR, we have monitored mRNA expression levels of c-fos, c-jun, JunB, c-myc, p53, H-ras, and histone H4 during the replicative senescen...

journal_title：Experimental cell research

authors： Irving J,Feng J,Wistrom C,Pikaart M,Villeponteau B

更新日期：1992-09-01 00:00:00

abstract：:Previous work has suggested that adhesive or cytoskeletal structures alter during epithelial transformation. The purpose of the present studies was to see whether such reorganizations were accompanied by consistent changes in the biophysical characteristics of cells. We used in vitro cell lines from rat liver and resp...

journal_title：Experimental cell research

authors： Heckman CA,Campbell AE,Wetzel B

更新日期：1987-03-01 00:00:00