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Ex vivo cultures of glioblastoma in three-dimensional hydrogel maintain the original tumor growth behavior and are suitable for preclinical drug and radiation sensitivity screening.

Abstract：

:Identification of new drugs and predicting drug response are major challenges in oncology, especially for brain tumors, because total surgical resection is difficult and radiation therapy or chemotherapy is often ineffective. With the aim of developing a culture system close to in vivo conditions for testing new drugs, we characterized an ex vivo three-dimensional culture system based on a hyaluronic acid-rich hydrogel and compared it with classical two-dimensional culture conditions. U87-MG glioblastoma cells and seven primary cell cultures of human glioblastomas were subjected to radiation therapy and chemotherapy drugs. It appears that 3D hydrogel preserves the original cancer growth behavior and enables assessment of the sensitivity of malignant gliomas to radiation and drugs with regard to inter-tumoral heterogeneity of therapeutic response. It could be used for preclinical assessment of new therapies.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Jiguet Jiglaire C,Baeza-Kallee N,Denicolaï E,Barets D,Metellus P,Padovani L,Chinot O,Figarella-Branger D,Fernandez C

doi

10.1016/j.yexcr.2013.12.010

subject

Has Abstract

pub_date

2014-02-15 00:00:00

pages

99-108

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(13)00527-2

journal_volume

321

pub_type

杂志文章

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