Abstract：
:We have examined the effects of diamide, an oxidizer of glutathione, on the progress of HeLa cells through the cell cycle. At concentrations which do not significantly alter generation time, anaphase or cytokinesis, diamide causes a two-fold increase in the duration of metaphase. At 3 X 10(-8) M, ouabain also prolongs metaphase without effect on anaphase or cytokinesis, though with a different time course. The data suggest that the metaphase stage of mitosis is particularly sensitive to alterations in both sulfhydryl groups and Na+ levels but that the effects of diamide are probably not primarily due to the oxidation of sulfhydryl groups of the Na+/K+-ATPase.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ziegler ML,Sisken JEdoi
10.1016/0014-4827(85)90449-5subject
Has Abstractpub_date
1985-05-01 00:00:00pages
263-6issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(85)90449-5journal_volume
158pub_type
杂志文章abstract：:The interfacial energy of the non-attached to substrate cell surface was analysed in tumor cell variants of the K-1735 melanoma and UV-2237 fibrosarcoma series, which exhibit distinct metastatic phenotypes. The highly metastatic cell variants exhibited a two-fold increase in the ability to form rapid cell substrate in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90625-6
更新日期：1984-08-01 00:00:00
abstract：:Phosphoinositide 3-kinases (PI3Ks) are an evolutionary conserved family of lipid kinases that control cell growth, metabolism and survival. By generating lipid second messengers that interact with specialized lipid-binding domains found in a wide spectrum of signaling molecules, PI3Ks instigate signaling through a net...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2013.02.021
更新日期：2013-05-15 00:00:00
abstract：:Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respective...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.01.012
更新日期：2012-03-10 00:00:00
abstract：:The importance of mononuclear leukocyte (MO) adhesion to dysfunctional endothelium and migration to the subendothelial space in the early phases of atherogenesis is well established. Few studies have addressed the relevance of polymorphonuclear leukocytes (PMNs) in the context of evolving lesions, and nothing is known...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5459
更新日期：2002-04-01 00:00:00
abstract：:Single cells from the siliceous sponge Geodia cydonium, obtained after chemical dissociation, reaggregate in the presence of the aggregation factor in Ca2+-containing medium to large aggregates. It was found that polyvalent organic cations (polylysine, spermine, spermidine, putrescine) enhance the Ca2+-mediated cell a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(78)90382-8
更新日期：1978-05-01 00:00:00
abstract：:The purpose of the present study was to determine if hemodynamic shear stress increases free cytosolic Ca2+ concentration ([Ca2+]i) of cultured pulmonary artery endothelial cells exposed to steady laminar fluid flow in a parallel plate chamber. Average [Ca2+]i was estimated by measuring cell-associated fura-2 fluoresc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90145-x
更新日期：1992-01-01 00:00:00
abstract：:This study aims to clarify the role and molecular mechanism of dynamin-related protein 1 (Drp1)-mediated mitochondrial homeostasis in high glucose (HG)-induced endometrial cancer (EC). Normal endometrium and tumor tissues of EC patients with normal and HG levels were collected, and Drp1 and p-Drp1 expression levels we...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.111880
更新日期：2020-04-01 00:00:00
abstract：:L1 cell adhesion molecule (L1-CAM) is a transmembrane cell adhesion molecule initially defined as a promigratory molecule in the developing nervous system. L1 is also overexpressed in a variety of human carcinomas and is associated with bad prognosis. In carcinoma cell lines L1 augments cell motility and metastasis, t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.04.004
更新日期：2008-08-01 00:00:00
abstract：:Gold nanoparticles are recently having much attention because of their increased applications in biomedical fields. In this paper, we demonstrated the photothermal efficacy of citrate capped gold nanoparticles (AuNPs) for the destruction of A431 cancer cells. Citrate capped AuNPs were synthesized successfully and char...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.04.010
更新日期：2011-08-15 00:00:00
abstract：:Osteopontin (OPN) is a multifunctional protein implicated in cellular adhesion and migration. Phosphorylation has emerged as a post-translational modification important for certain biological activities of OPN. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.10.019
更新日期：2010-02-01 00:00:00
abstract：:The microtubule cytoskeleton is known to play a role in cell structure and serve as a scaffold for a variety of active molecules in processes as diverse as motility and cell division. The literature on the role of microtubules in signal transduction, however, is marked by inconsistencies. We have investigated a well-s...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.08.020
更新日期：2009-11-15 00:00:00
abstract：:In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：
更新日期：1996-07-10 00:00:00
abstract：:The DNA topoisomerase I inhibitor camptothecin (CAM) is selectively cytotoxic to S-phase cells of HL-60, and some other myelogenous leukemic lines. The early effects of cell exposure to 0.05-0.2 micrograms/ml CAM are seen after 2 h; at that time a progressive degradation of DNA in the chromatin of S-phase cells is ini...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90534-2
更新日期：1991-03-01 00:00:00
abstract：:Tyrosine-specific protein kinase activity of pp60c-src was examined in human Y79 retinoblastoma cells cultured in monolayers after clusters in suspension culture had been dissociated. The activity increased five- to six-fold between Days 1 and 7 in the monolayer cultures, with a concomitant increase in numbers of cell...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90255-9
更新日期：1990-07-01 00:00:00
abstract：:In dog thyrocytes in primary culture, thyrotropin (TSH), acting through cyclic AMP, induces proliferation and differentiation expression, while tetradecanoylphorbol acetate (TPA) or epidermal growth factor (EGF) induces proliferation and dedifferentiation. In this work, we have investigated the regulation of mRNA expr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1294
更新日期：1994-10-01 00:00:00
abstract：:The mitochondrion imports and processes the vast majority of the proteins that constitute its structural elements and metabolic pathways. To study mitochondrial precursor processing in the context of the cellular environment, we employed the baculovirus expression system to overexpress the prototypical precursor prote...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4331
更新日期：1999-02-01 00:00:00
abstract：:The cellular plasma membrane becomes permeable to macromolecules during the cell injury process. This results in exposure of the interior of the cell to plasma proteins and to high-affinity binding of the Fc part of IgG to intermediate filaments (Hansson, G K, Starkebaum, G A, Benditt, E P & Schwartz, S M, Proc natl a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90311-9
更新日期：1987-06-01 00:00:00
abstract：:Laminins assemble into trimers composed of alpha, beta, and gamma chains which posttranslationally are glycosylated and sometimes proteolytically cleaved. In the current paper we set out to characterize posttranslational modifications and the laminin isoforms formed by laminin alpha1 and alpha5 chains. Comparative pul...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4980
更新日期：2000-09-15 00:00:00
abstract：:Previous work has suggested that adhesive or cytoskeletal structures alter during epithelial transformation. The purpose of the present studies was to see whether such reorganizations were accompanied by consistent changes in the biophysical characteristics of cells. We used in vitro cell lines from rat liver and resp...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90232-1
更新日期：1987-03-01 00:00:00
abstract：:SMN, the affected protein in spinal muscular atrophy (SMA), is a cytoplasmic protein that also occurs in nuclear structures called "gems" and is involved in snRNP maturation. Coilin-p80 is a marker protein for nuclear Cajal bodies (coiled bodies; CBs) which are also involved in snRNP maturation, storage or transport. ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5186
更新日期：2001-05-01 00:00:00
abstract：:For cancer metastasis, tumor cells present in the circulation must first adhere to the endothelium. Integrins play a central role in leukocyte adhesion to the endothelium and subsequent migration into tissues. The majority of tumor cells derived from solid cancers, including breast cancer, do not express integrins. We...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00184-8
更新日期：2003-08-01 00:00:00
abstract：:Bax, a multi-domain pro-apoptotic Bcl-2 family member, is a key regulator for the release of apoptogenic factors from mitochondria. MOAP-1, which was first isolated from a screen for Bax-associating proteins, interacts with Bax upon apoptotic induction. MOAP-1 is a short-lived protein that is constitutively degraded b...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.11.021
更新日期：2009-04-15 00:00:00
abstract：:Tubular epithelial-to-mesenchymal transition (EMT) plays a crucial role in the progression of renal tubular interstitial fibrosis (TIF), which subsequently leads to chronic kidney disease (CKD) and eventually, end-stage renal disease (ESRD). We propose that augmenter of liver regeneration (ALR), a member of the newly ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.07.001
更新日期：2014-10-01 00:00:00
abstract：:tsJT60, a temperature-sensitive (ts) cell-cycle mutant of Fischer rats, is viable at both the permissive (34 degrees C) and nonpermissive (40 degrees C) temperatures. The cells grow normally in exponential growth phase at both temperatures, but when stimulated with serum from G0 phase they enter S phase at 34 degrees ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90347-3
更新日期：1988-11-01 00:00:00
abstract：:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4570
更新日期：1999-08-25 00:00:00
abstract：:Identification of breast cancer not being a single disease but backed by multiple heterogeneous oncogenic subpopulations is of growing interest in developing personalized therapies to provide optimal outcomes. Through this review, we bring attention to evolution of tumor and microenvironment heterogeneity as a predomi...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2013.09.002
更新日期：2014-01-01 00:00:00
abstract：:Identification of transcription factors expressed by differentiated cells is informative not only of tissue-specific pathways, but to help identify master regulators for cellular reprogramming. If applied, such an approach could generate healthy autologous tissue-specific cells for clinical use where cells from the ho...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.05.031
更新日期：2018-08-15 00:00:00
abstract：:Distal-less homeobox 5 (Dlx5) is a pro-osteogenic but anti-adipogenic transcription factor that regulates lineage commitment in mesenchymal stem cells. Although the expression of Dlx5 is known to be decreased by adipogenic stimuli, the mechanism of Dlx5 down-regulation has not yet been clarified. MicroRNAs (miRNAs) ar...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.04.020
更新日期：2013-08-15 00:00:00
abstract：:Fibroblast proliferation and procollagen production are central features of tissue repair and fibrosis. In addition to its role in blood clotting, the coagulation cascade proteinase thrombin can contribute to tissue repair by stimulating fibroblasts via proteolytic activation of proteinase-activated receptor-1 (PAR1)....
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.021
更新日期：2005-03-10 00:00:00
abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111719
更新日期：2020-01-01 00:00:00
