Abstract：
:In dog thyrocytes in primary culture, thyrotropin (TSH), acting through cyclic AMP, induces proliferation and differentiation expression, while tetradecanoylphorbol acetate (TPA) or epidermal growth factor (EGF) induces proliferation and dedifferentiation. In this work, we have investigated the regulation of mRNA expression of the protooncogene jun B in these cells. TSH stimulated jun B expression very transiently with biphasic kinetics similar to those obtained for c-myc mRNA accumulation. Forskolin reproduced these effects, suggesting that they are, as other effects of TSH in this system, mediated by cyclic AMP. As shown by nuclear run-on experiments, jun B is regulated by the cAMP pathway at the transcriptional level. As in other cell types, EGF or TPA caused a more sustained increase in mRNA levels. In thyroid slices, in which DNA synthesis appears to be induced by the wounding process, jun B is also induced, suggesting a correlation with the proliferative status of the cell. Interestingly, two jun B mRNAs of 2.1 and 2.3 kb were induced by all the mitogenic pathways. The kinetics of their accumulation were different; i.e., TPA induced the smaller transcript with some delay after the longer one and cycloheximide induced the progressive shortening of the first appearing heavier mRNA. The 2.3-kb messenger has a longer poly(A) tail, and kinetics in the presence of actinomycin D suggested it could represent a precursor form of the 2.1-kb messenger. It is suggested that the specific kinetics of cyclic-AMP-induced accumulation of jun B mRNA could be related to the dual stimulation of differentiation and proliferation by TSH in dog thyrocytes.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Pirson I,Dumont JEdoi
10.1006/excr.1994.1294subject
Has Abstractpub_date
1994-10-01 00:00:00pages
561-9issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(84)71294-8journal_volume
214pub_type
杂志文章abstract：:The tumor suppressor protein p16INK4a (inhibitor of CDK4) is one of the most direct links between cell-cycle control and cancer. The p16INK4a gene is frequently inactivated in human tumors, and inheritance of mutant alleles results in susceptibility to several types of cancer. p16INK4a is part of a cell-cycle regulato...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1997.3824
更新日期：1997-11-25 00:00:00
abstract：:Raver2 was originally identified as a member of the hnRNP family through database searches revealing three N-terminal RNA recognition motifs (RRMs) bearing highest sequence identity in the RNP sequences to the related hnRNP Raver1. Outside the RRM region, both Raver proteins are quite divergent in sequence except for ...
journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2009.11.023
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2017.11.025
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.09.021
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abstract：:In this study we have analyzed short- and long-term changes in extracellular signal-regulated kinase (ERK) 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of human promyelocytic leukemia cells. Immunoprecipitation of HL-60 cellular extracts with an ERK antibody followed by in...
journal_title：Experimental cell research
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doi：10.1006/excr.1997.3631
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journal_title：Experimental cell research
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更新日期：1992-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4773
更新日期：2000-02-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.5055
更新日期：2000-12-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.08.012
更新日期：2014-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.01.010
更新日期：2009-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90182-a
更新日期：1990-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.06.005
更新日期：2008-08-15 00:00:00
abstract：:Defects in the regulation of programmed cell death play a fundamental role in the development of neoplasia and neurological disorders, both of which are linked to the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection. We previously showed that the HTLV-1 Tax protein protects from apoptosis induced by seru...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2006-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.10.033
更新日期：2006-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90201-2
更新日期：1989-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4530
更新日期：1999-07-10 00:00:00
abstract：:Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate w...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90042-0
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1983-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4586
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2018-09-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4156
更新日期：1998-10-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.018
更新日期：2006-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1306
更新日期：1993-12-01 00:00:00
abstract：:High glucose concentrations associated with diabetes have been shown to cause the nonenzymatic modification of proteins. Reducing sugars covalently bind to free amine groups, undergo Amadori rearrangements, and crosslink with other glucose-modified proteins. Crosslinking of type I collagen by incubation with different...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0308
更新日期：1996-10-10 00:00:00
abstract：:Co-cultures of lutropin receptor (LHR) positive and negative Leydig cells were used to test the hypothesis that the LHR provokes phosphorylation of the extracellular regulated kinases (ERK1/2) using intracellular and intercellular pathways. Addition of hCG to MA-10 cells (LHR positive) stimulates phosphorylation of th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.06.025
更新日期：2008-01-01 00:00:00
abstract：:In this study an amphotropic retrovirus has been used to efficiently transduce normal human (NF) and scleroderma (systemic sclerosis; SSc) dermal fibroblasts (SScF) with a sequence encoding a temperature-sensitive mutant of the SV40 large T antigen (tsA58-U19). From the primary outgrowths of skin explants, cultures we...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1332
更新日期：1995-10-01 00:00:00
abstract：:We have investigated whether restriction endonucleases produce bands on human chromosomes by extracting DNA, using staining methods which are stoichiometric for DNA. Restriction enzymes that produce C-band patterns appear to remove DNA extensively from chromosome arms. In general, however, those restriction enzymes th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90509-9
更新日期：1985-11-01 00:00:00
abstract：:The epidermal growth factor (EGF) receptor is well known as a mediator of mitogenic signaling and its tyrosine kinase activity has been suggested as a viable target in cancer chemotherapy. To explore the consequences of abolishing the kinase activity of this receptor, we have utilized a potent and specific inhibitor o...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3571
更新日期：1997-06-15 00:00:00
