Abstract：
:We have isolated a clone of human lymphoblastoid cells that is capable of undergoing the phenomenon of contact-mediated cell spreading in vitro. We have detected this behavior when using both transmission electron microscopy (TEM), and differential interference contrast microscopy. Upon cell-cell contact, cells become loosely adherent and then begin to extend cellular processes that contact other cells and the substrate. We have also selected a variant clone that has lost the capability for cell spreading. The adhesions-defective variant becomes adhesion-positive and appears morphologically identical with the adhesive cells only in response to specific amino sugars. In the presence of those sugars the adhesion response is correlated with a shift in the apparent molecular weight of an iodinatable component. We propose that contact-mediated cell spreading in lymphoblastoid cells is mediated by a non-transferable cell surface-associated glycoconjugate. The synthesis of that glycoconjugate is defective in the non-adhesive clone, unless the cells are grown in glucosamine or mannosamine.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Sorrentino JA,Millis AJ,Whipple AP,Colquhoun WRdoi
10.1016/0014-4827(83)90015-0subject
Has Abstractpub_date
1983-05-01 00:00:00pages
369-79issue
2eissn
0014-4827issn
1090-2422journal_volume
145pub_type
杂志文章abstract：:Transcription profiling of early embryos emphasizes that differential gene expression is a fundamental control mechanism of development. Precise regulatory mechanisms operate on a background of epigenetic changes in chromatin composition, modification, and architecture that are integral for the development of a plurip...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2005.06.023
更新日期：2005-10-01 00:00:00
abstract：:We have studied the cytotoxicity of ricin in cells treated with brefeldin A (BFA), which dramatically disrupts the structure of the Golgi apparatus causing Golgi content and membrane to redistribute to the ER. BFA inhibits the cytotoxicity of ricin in Chinese hamster ovary, normal rat kidney, and Vero cells and abolis...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90056-z
更新日期：1991-02-01 00:00:00
abstract：:Two genes, KIN1 and KIN2, were isolated in Saccharomyces cerevisiae by their homology to the protein kinase family of viral oncogenes. In previous studies, we identified the yeast KIN1 gene product (pp145KIN1) and KIN2 gene product (pp145KIN2) as 145 kDa phosphoproteins with serine/threonine protein kinase activity. W...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1177
更新日期：1994-07-01 00:00:00
abstract：:The activation of Cdc2 kinase induces the entry into M-phase of all eukaryotic cells. We have developed a cell-free system prepared from prophase-arrested Xenopus oocytes to analyze the mechanism initiating the all-or-none activation of Cdc2 kinase. Inhibition of phosphatase 2A, the major okadaic acid-sensitive Ser/Th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4220
更新日期：1998-11-01 00:00:00
abstract：:In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：
更新日期：1996-07-10 00:00:00
abstract：:Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.01.025
更新日期：2018-03-01 00:00:00
abstract：:Distal-less homeobox 5 (Dlx5) is a pro-osteogenic but anti-adipogenic transcription factor that regulates lineage commitment in mesenchymal stem cells. Although the expression of Dlx5 is known to be decreased by adipogenic stimuli, the mechanism of Dlx5 down-regulation has not yet been clarified. MicroRNAs (miRNAs) ar...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.04.020
更新日期：2013-08-15 00:00:00
abstract：:DNA topoisomerase (topo) I plays an important role in DNA metabolism by relieving the torsional restraints of DNA topology through ATP-independent single-strand DNA breakage. In the present study, we expressed human topo I in HeLa cells by fusing it to enhanced green fluorescent protein (EGFP). The EGFP-topo I fusion ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4864
更新日期：2000-05-01 00:00:00
abstract：:The effect of RU 486, a synthetic steroid that is a powerful antagonist of glucocorticoid hormones, was tested on the transcription of several glucocorticoid-regulated genes in different cell types: inflammatory murine macrophages and two human mammary gland-derived cell lines, MDA-MB-231 and HBL-100. The transcriptio...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90282-5
更新日期：1987-12-01 00:00:00
abstract：:In this study, we demonstrate that protein kinase C (PKC) activators, including phorbol-12-myristate-13-acetate (PMA), 1,2-dioctanoyl-sn-glycerol (DOG), and platelet-derived growth factor alpha are potent inducers of angiopoietin-like protein 4 (ANGPTL4) expression in several normal lung cell types and carcinoma cell ...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2010-02-15 00:00:00
abstract：:Mast cells are critical regulators of the pathogenesis of the central nervous system diseases, including stroke, multiple sclerosis, and traumatic brain injury, and brain tumors. Here, we have summarized the literature data concerning the involvement of mast cells in blood-brain barrier alterations, and we have sugges...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2015.05.013
更新日期：2015-10-15 00:00:00
abstract：BACKGROUND:Müller cell gliosis not only plays an important physiological role by maintaining retinal neuronal homeostasis but is also associated with multiple pathological events in the retina, including optic nerve crush (ONC) injury. Modulating Müller cell gliosis contributes to the creation of a permissive environme...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.01.009
更新日期：2019-02-15 00:00:00
abstract：:Basic fibroblast growth factor (bFGF) is a mitogen and a survival factor in fibroblasts and endothelial cells. It acts as an angiogenesis factor in breast cancer, but paradoxically inhibits proliferation in several breast cancer cell lines. In this study, we investigated the effects of bFGF on the survival of MCF-7 hu...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3820
更新日期：1998-01-10 00:00:00
abstract：:The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1993.1022
更新日期：1993-02-01 00:00:00
abstract：:Skeletal muscle differentiation is strongly inhibited by transforming growth factor type beta (TGF-beta), although muscle formation as well as regeneration normally occurs in an environment rich in this growth factor. In this study, we evaluated the role of intracellular regulatory Smads proteins as well as TGF-beta-r...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.04.031
更新日期：2010-09-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2010.05.019
更新日期：2010-11-01 00:00:00
abstract：:Rabbit smooth muscle cells (SMC) in primary culture attached to and started proliferating on native and heat-denatured type I collagens, although the amount of cell attachment to denatured collagen was significantly lower. The cells adhered poorly and were unable to grow on commercial gelatin. In contrast, synthetic S...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1225
更新日期：1995-07-01 00:00:00
abstract：:The results of Phase I/II clinical trials indicate that ricin A-chain-containing immunotoxins cause vascular leak syndrome, characterized by hypoalbuminemia with resultant weight gain and edema. Vascular leak syndrome may be a dose-limiting factor during treatment with ricin A-chain-containing immunotoxins. In this re...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1142
更新日期：1993-06-01 00:00:00
abstract：:Chromosomal DNA fiber replication was investigated in the shoot meristem of mustard plants during the morphogenetic transition from the leaf-forming (vegetative) to the flower-forming (evoked) condition. The replicon size, determined using the modal class, was 15 micron in the vegetative meristem and shifted to 7.5 mi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90283-2
更新日期：1988-12-01 00:00:00
abstract：:Small vertebrate organisms have emerged as key players in the post-genomic era for the functional characterization of novel genes on a high-throughput scale. In this context, the zebrafish embryos and Xenopus tadpoles represent attractive and valuable models to rapidly identify and characterize novel genes involved in...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2005.10.018
更新日期：2006-03-10 00:00:00
abstract：:The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5510
更新日期：2002-06-10 00:00:00
abstract：:We developed an improved method for isolation of peroxisome biogenesis-defective somatic animal cell mutants, using a combination of green fluorescent protein (GFP) expression and the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) selection method. We used TKaG1 and TKaG2 cells, the wild-type Chinese hamster ovary (CHO) c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4413
更新日期：1999-05-01 00:00:00
abstract：:It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5222
更新日期：2001-06-10 00:00:00
abstract：:Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.2000.5039
更新日期：2000-11-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90436-6
更新日期：1983-03-01 00:00:00
abstract：:Alexander disease (AxD) is a rare neurodegenerative disorder characterized by large cytoplasmic aggregates in astrocytes and myelin abnormalities and caused by dominant mutations in the gene encoding glial fibrillary acidic protein (GFAP), the main intermediate filament protein in astrocytes. We tested the effects of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.04.035
更新日期：2007-08-01 00:00:00
abstract：:Angiogenesis plays important roles in solid tumors progression. Growth factors such as vascular endothelial growth factors (VEGFs) can induce angiogenesis and hypoxia promotes the expression of VEGFs through activating hypoxia-inducible factor 1 (HIF-1α). However, the regulation of HIF-1α still not been fully understo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111495
更新日期：2019-10-01 00:00:00
abstract：:To study the assembly of intermediate filaments in vivo we have transfected fibroblast cell lines with the cDNAs coding for keratins 8 and 18 under the control of the promoter of the SV40 early region and followed keratin expression by RNA hybridization, two-dimensional gel electrophoresis, and immunofluorescence anal...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90274-1
更新日期：1988-12-01 00:00:00
abstract：:In many systems, events participating in cell division are controlled by intracellular pH (pHi). In Xenopus eggs, fertilization is accompanied by an increase in pHi which occurs concomitantly with an increase in protein synthesis and a reinitiation of DNA synthesis, leading the embryo to cell division. In this paper, ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90313-3
更新日期：1989-12-01 00:00:00
abstract：:The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.12.015
更新日期：2007-03-10 00:00:00
