Abstract:
:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus early development when 12 rapid cell cycles occur without transcription of the genome. We show that after fertilization, part of the large store of p53 previously stored in the cytoplasm of the oocyte is imported into the nucleus. This import was further analyzed in relation with DNA replication and DNA repair using cell-free systems from Xenopus eggs. Formation of a nuclear lamina envelope is necessary for the import of p53 into the nucleus. p53 associates both with decondensed DNA and the nuclear lamina envelope, but no colocalization with prereplication or replication complexes is observed. We show that UV- or gamma-damaged nuclei recruit p53 as well as replication protein A (RPA) in large common foci. Together, these data suggest that p53 plays a role in the regulation of the accelerated S phases that occur during Xenopus early development, in a manner that does not rely on its transcription-mediated activity.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Tchang F,Méchali Mdoi
10.1006/excr.1999.4570keywords:
subject
Has Abstractpub_date
1999-08-25 00:00:00pages
46-56issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(99)94570-6journal_volume
251pub_type
杂志文章abstract::The Golgi apparatus undergoes extensive fragmentation during mitosis in animal cells. Protein kinases play a critical role during fragmentation of the Golgi apparatus. We reported here that Polo-like kinase 3 (Plk3) may be an important mediator during Golgi breakdown. Specifically, Plk3 was concentrated at the Golgi a...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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pub_type: 杂志文章
doi:10.1006/excr.1994.1176
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更新日期:1990-05-01 00:00:00
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更新日期:2003-04-01 00:00:00
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journal_title:Experimental cell research
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更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0014-4827(87)90262-x
更新日期:1987-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2014.08.017
更新日期:2014-12-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.09.010
更新日期:2004-01-15 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:2009-07-15 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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pub_type: 杂志文章
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abstract::The distribution of rDNA and the uptake of tritiated uridine was investigated in nucleoli of human Sertoli cells. The nucleolar components in these cells are spatially arranged in a highly ordered and invariable way and can be recognized in both light and electron microscopy. The pattern of distribution of rDNA and th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90364-9
更新日期:1989-09-01 00:00:00