Abstract:
:Cell union in mating pairs in the ciliate Euplotes raikovi is controlled by a system of multiple mating types which are inherited with alleles codominant at the genetic locus mat and expressed via diffusible mating pheromones. The mating pheromones Er-2, Er-3, and Er-11 were purified from cells homozygous for the mat-2, mat-3, and mat-11 alleles, respectively. These pheromones are proteins of similar Mr (11,000-12,000) and acidity (pI 3.7-4.0) and are active at a concentration that varies from 2.9 X 10(-12) to 1.2 X 10(-11) M. Data on amino acid composition revealed that an unusually high amount of cysteine (12-15.7%) and poor contents of basic amino acids are common to every pheromone. On the basis of this uniformity in the main biochemical traits, which also holds for the previously purified pheromone Er-1, it was concluded that E. raikovi mating pheromones are members of a family of proteins structurally diversified from each other to varying extents.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Raffioni S,Miceli C,Concetti A,Barra D,Luporini Pdoi
10.1016/0014-4827(87)90399-5subject
Has Abstractpub_date
1987-10-01 00:00:00pages
417-24issue
2eissn
0014-4827issn
1090-2422journal_volume
172pub_type
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