Purification and characterization of new mating pheromones of the ciliate Euplotes raikovi.

Abstract:

:Cell union in mating pairs in the ciliate Euplotes raikovi is controlled by a system of multiple mating types which are inherited with alleles codominant at the genetic locus mat and expressed via diffusible mating pheromones. The mating pheromones Er-2, Er-3, and Er-11 were purified from cells homozygous for the mat-2, mat-3, and mat-11 alleles, respectively. These pheromones are proteins of similar Mr (11,000-12,000) and acidity (pI 3.7-4.0) and are active at a concentration that varies from 2.9 X 10(-12) to 1.2 X 10(-11) M. Data on amino acid composition revealed that an unusually high amount of cysteine (12-15.7%) and poor contents of basic amino acids are common to every pheromone. On the basis of this uniformity in the main biochemical traits, which also holds for the previously purified pheromone Er-1, it was concluded that E. raikovi mating pheromones are members of a family of proteins structurally diversified from each other to varying extents.

journal_name

Exp Cell Res

authors

Raffioni S,Miceli C,Concetti A,Barra D,Luporini P

doi

10.1016/0014-4827(87)90399-5

subject

Has Abstract

pub_date

1987-10-01 00:00:00

pages

417-24

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

172

pub_type

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