Inhibition of cell proliferation by the cholecystokinin antagonist L-364,718.

abstract：:RNA polymerase II transcripts are confined to nuclear compartments. A detailed analysis of the nuclear topology of RNA from individual genes was performed for transcripts from the marker gene coding for chloramphenicol acetyltransferase, expressed at a high level from the HTLV-1 LTR promoter. The construct was transfe...

journal_title：Experimental cell research

authors： Bridger JM,Kalla C,Wodrich H,Weitz S,King JA,Khazaie K,Kräusslich HG,Lichter P

更新日期：2005-01-15 00:00:00

abstract：:The interaction of fluorescein-labeled nerve growth factor (NGF) with human melanoma cells (A875) has been studied in order to assess better methodology for rigorous NGF binding studies. The NGF was modified at a single carboxyl group with iodoacetamidofluorescein after reaction with carbodiimide and cystamine. The mo...

journal_title：Experimental cell research

authors： Kasaian MT,Jacobberger JW,Neet KE

更新日期：1994-01-01 00:00:00

abstract：:Fenretinide is an effective inducer of apoptosis in many malignancies but its precise mechanism(s) of action in the induction of apoptosis in neuroblastoma is unclear. To characterize fenretinide-induced apoptosis, neuroblastoma cell lines were treated with fenretinide and flow cytometry was used to measure apoptosis,...

journal_title：Experimental cell research

authors： Lovat PE,Ranalli M,Annichiarrico-Petruzzelli M,Bernassola F,Piacentini M,Malcolm AJ,Pearson AD,Melino G,Redfern CP

更新日期：2000-10-10 00:00:00

abstract：:Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...

journal_title：Experimental cell research

authors： Paillasson S,Van De Corput M,Dirks RW,Tanke HJ,Robert-Nicoud M,Ronot X

更新日期：1997-02-25 00:00:00

abstract：:Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antib...

journal_title：Experimental cell research

authors： Vasilikaki-Baker H,Nishioka Y

更新日期：1983-08-01 00:00:00

abstract：:The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase pro...

journal_title：Experimental cell research

authors： Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KH

更新日期：1983-12-01 00:00:00

abstract：:Accumulating evidence shows that the inhibition of thromboxane synthase (TXS) induced apoptosis in cancer cells. TXS inhibitor 1-Benzylimidzole (1-BI) can trigger apoptosis in lung cancer cells but the mechanism is not fully defined. In this study, lung cancer cells were treated with 1-BI. In this study, the level of ...

journal_title：Experimental cell research

authors： Leung KC,Li MY,Leung BC,Hsin MK,Mok TS,Underwood MJ,Chen GG

更新日期：2010-12-10 00:00:00

abstract：:Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 delta isoform (PP1cdelta) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for regulating the extent of myosin light chain phosphorylation. Here we report the i...

journal_title：Experimental cell research

authors： Twomey E,Li Y,Lei J,Sodja C,Ribecco-Lutkiewicz M,Smith B,Fang H,Bani-Yaghoub M,McKinnell I,Sikorska M

更新日期：2010-01-01 00:00:00

abstract：:The pre-mRNA splicing factor 4 kinase PRP4K (PRPF4B), is an essential kinase that is a component of the U5 snRNP and functions in spliceosome assembly. We demonstrated that PRP4K is a novel biological marker for taxane response in ovarian cancer patients and reduced levels of PRP4K correlate with intrinsic and acquire...

journal_title：Experimental cell research

authors： Lahsaee S,Corkery DP,Anthes LE,Holly A,Dellaire G

更新日期：2016-01-01 00:00:00

abstract：:This study shows the sensitivity of insulin-secreting RIN m5F cells to undergoing apoptosis without modifying intracellular free calcium concentrations. The culture of cells in the absence of serum during 6 h failed to produce spontaneous DNA fragmentation. However, when cultures were carried out in the presence of th...

journal_title：Experimental cell research

authors： Sánchez-Margalet V,Lucas M,Solano F,Goberna R

更新日期：1993-11-01 00:00:00

abstract：:The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...

journal_title：Experimental cell research

authors： Mallia CM,Aguirre V,McGary E,Tang Y,Scandurro AB,Liu C,Noguchi CT,Beckman BS

更新日期：1999-02-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is one of the most common cancers diagnosed worldwide. However, the mechanism underlying HCC pathogenesis remains unknown. In the present study, TRIM24 was found increased in human HCC clinical samples and positively correlated with HCC tumor grade. Furthermore, TRIM24 knockdown inhibits...

journal_title：Experimental cell research

authors： Zhu Y,Zhao L,Shi K,Huang Z,Chen B

更新日期：2018-06-15 00:00:00

abstract：:Proliferation of human B- and T-lymphoid cell lines including Raji and Akata cells was found to be arrested at the G1 stage in the cell cycle by dimethyl sulfoxide (DMSO). The G1 arrest by DMSO occurred gradually and was completed within 96 h after addition of 1.5% DMSO concomitantly with a decrease in growth rate. Pr...

journal_title：Experimental cell research

authors： Sawai M,Takase K,Teraoka H,Tsukada K

更新日期：1990-03-01 00:00:00

abstract：:Embryonic stem (ES) cells have been tested for potential cell transplantation therapy for CNS disorders. Understanding their differentiation mechanism and identifying factors involved in driving excitatory and inhibitory neuron lineages should enhance the efficacy and efficiency of the cell transplantation therapy. We...

journal_title：Experimental cell research

authors： Theus MH,Wei L,Francis K,Yu SP

更新日期：2006-10-01 00:00:00

abstract：:In the trabecular meshwork (TM) of the eye, regulation of tissue contractility by the PPRARI sequence within the Heparin II (HepII) domain of fibronectin is believed to control the movement of aqueous humor and dictate the level of intraocular pressure. This study shows that the HepII domain utilizes activated alpha4b...

journal_title：Experimental cell research

authors： Schwinn MK,Gonzalez JM Jr,Gabelt BT,Sheibani N,Kaufman PL,Peters DM

更新日期：2010-05-15 00:00:00

abstract：:Our results showed that the expression of the src gene in chick embryo fibroblasts (CEF) released the density-dependent inhibition (DDI) of phosphate metabolism (phosphate uptake and phosphorylation of small organic compounds). With increasing cell density, phosphate metabolism decreased by 58% in normal CEF and, in c...

journal_title：Experimental cell research

authors： Jullien M,Harel L,Golde A,Villaudy J,Pugnet P

更新日期：1984-06-01 00:00:00

abstract：:Transcription profiling of early embryos emphasizes that differential gene expression is a fundamental control mechanism of development. Precise regulatory mechanisms operate on a background of epigenetic changes in chromatin composition, modification, and architecture that are integral for the development of a plurip...

journal_title：Experimental cell research

authors： Meehan RR,Dunican DS,Ruzov A,Pennings S

更新日期：2005-10-01 00:00:00

abstract：:The characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wave-length microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cyt...

journal_title：Experimental cell research

authors： Tsunoda Y

更新日期：1990-06-01 00:00:00

abstract：:Keratinocyte growth factor (KGF/FGF7) and fibroblast growth factor 10 (FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is...

journal_title：Experimental cell research

authors： Ceccarelli S,Cardinali G,Aspite N,Picardo M,Marchese C,Torrisi MR,Mancini P

更新日期：2007-05-15 00:00:00

abstract：:Huntingtin interacting protein HYPK (Huntingtin Yeast Partner K) is an intrinsically unstructured protein having chaperone-like activity and can suppress mutant huntingtin aggregates and toxicity in cell model of Huntington's Disease (HD). Heat shock response is an adaptive mechanism of cells characterized by upregula...

journal_title：Experimental cell research

authors： Das S,Bhattacharyya NP

更新日期：2016-05-01 00:00:00

abstract：:Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model - all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fiber...

journal_title：Experimental cell research

authors： Liu Z,Ho CH,Grinnell F

更新日期：2014-08-15 00:00:00

abstract：:Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...

journal_title：Experimental cell research

authors： Halleck MS,Schlegel RA

更新日期：1983-09-01 00:00:00

abstract：:Fluid-phase endocytosis was significantly faster in three lines of human lysosomal storage disease fibroblasts (two I cells and one Sandhoff's) than in three normals. Re-exocytosis of fluid showed similar kinetics in all lines when release was expressed as a percentage of the radioactivity associated with the cells at...

journal_title：Experimental cell research

authors： Dean RT

更新日期：1984-04-01 00:00:00

abstract：:Several cell division cycle (cdc) mutants of Saccharomyces cerevisiae (cdc28, cdc35, cdc19, cdc21, and cdc17) at the restrictive temperature (37 degrees C) in the presence of 1% glucose and defined medium divert most of the carbon (approximately 50%) to ethanol production with low biomass growth yields (Yglc) that cor...

journal_title：Experimental cell research

authors： Mónaco ME,Valdecantos PA,Aon MA

更新日期：1995-03-01 00:00:00

abstract：:We have isolated a clone of human lymphoblastoid cells that is capable of undergoing the phenomenon of contact-mediated cell spreading in vitro. We have detected this behavior when using both transmission electron microscopy (TEM), and differential interference contrast microscopy. Upon cell-cell contact, cells become...

journal_title：Experimental cell research

authors： Sorrentino JA,Millis AJ,Whipple AP,Colquhoun WR

更新日期：1983-05-01 00:00:00

abstract：:Rat IPC-81 promyelocytic leukemia cells responded to cAMP analog by undergoing apoptotic cell death both when anchored to fibronectin and when free in the medium. The protein kinase C stimulator 12-O-tetradecanoylphorbol 13-acetate enhanced the anchoring to substratum without impeding cAMP-induced cell death. The immo...

journal_title：Experimental cell research

authors： Vintermyr OK,Gjertsen BT,Lanotte M,Døskeland SO

更新日期：1993-05-01 00:00:00

abstract：:Dipeptidyl peptidase IV (DPPIV) is a serine peptidase that cleaves N-terminal dipeptides from polypeptides when the second residue is a proline or an alanine. We have recently cloned cDNAs for rat gp110, a membrane glycoprotein with Mr of 110,000 isolated initially from rat liver. Studies reported here establish that ...

journal_title：Experimental cell research

authors： Hong WJ,Petell JK,Swank D,Sanford J,Hixson DC,Doyle D

更新日期：1989-05-01 00:00:00

abstract：:LC3, a mammalian homologue of yeast Atg8, is assumed to play an important part in bulk sequestration and degradation of cytoplasm (macroautophagy), and is widely used as an indicator of this process. To critically examine its role, we followed the autophagic flux of LC3 in rat hepatocytes during conditions of maximal ...

journal_title：Experimental cell research

authors： Szalai P,Hagen LK,Sætre F,Luhr M,Sponheim M,Øverbye A,Mills IG,Seglen PO,Engedal N

更新日期：2015-04-10 00:00:00

abstract：:Several lines of evidence indicate that telomere shortening during in vitro aging of human somatic cells plays a causal role in cellular senescence. A critical telomere length seems to be associated with the replicative block characterizing senescent cells. In this paper we analyzed the mean length of the terminal res...

journal_title：Experimental cell research

authors： Mondello C,Petropoulou C,Monti D,Gonos ES,Franceschi C,Nuzzo F

更新日期：1999-04-10 00:00:00

abstract：:A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

journal_title：Experimental cell research

authors： Moore SA

更新日期：1987-08-01 00:00:00