Abstract:
:Accumulating evidence shows that the inhibition of thromboxane synthase (TXS) induced apoptosis in cancer cells. TXS inhibitor 1-Benzylimidzole (1-BI) can trigger apoptosis in lung cancer cells but the mechanism is not fully defined. In this study, lung cancer cells were treated with 1-BI. In this study, the level of reactive oxygen species (ROS) was measured and NF-κB activity was determined in human lung cancer cells. The roles of ROS and NF-κB in 1-BI-mediated cell death were analyzed. The results showed that 1-BI induced ROS generation but decreased the activity of NF-κB by reducing phosphorylated IκBα (p-IκBα) and inhibiting the translocation of p65 into the nucleus. In contrast to 1-BI, antioxidant N-acetyl cysteine (NAC) stimulated cell proliferation and significantly protected the cells from 1-BI-mediated cell death by neutralizing ROS. Collectively, apoptosis induced by 1-BI is associated with the over-production of ROS and the reduction of NF-κB. Antioxidants can significantly block the inhibitory effect of 1-BI.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Leung KC,Li MY,Leung BC,Hsin MK,Mok TS,Underwood MJ,Chen GGdoi
10.1016/j.yexcr.2010.07.003subject
Has Abstractpub_date
2010-12-10 00:00:00pages
3468-77issue
20eissn
0014-4827issn
1090-2422pii
S0014-4827(10)00365-4journal_volume
316pub_type
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