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Focal adhesion kinase regulates cell-cell contact formation in epithelial cells via modulation of Rho.

Abstract：

:Focal Adhesion Kinase (FAK) is a non-receptor tyrosine kinase that plays a key role in cellular processes such as cell adhesion, migration, proliferation and survival. Recent studies have also implicated FAK in the regulation of cell-cell adhesion. Here, evidence is presented showing that siRNA-mediated suppression of FAK levels in NBT-II cells and expression of dominant negative mutants of FAK caused loss of epithelial cell morphology and inhibited the formation of cell-cell adhesions. Rac and Rho have been implicated in the regulation of cell-cell adhesions and can be regulated by FAK signaling. Expression of active Rac or Rho in NBT-II cells disrupted formation of cell-cell contacts, thus promoting a phenotype similar to FAK-depleted cells. The loss of intercellular contacts in FAK-depleted cells is prevented upon expression of a dominant negative Rho mutant, but not a dominant negative Rac mutant. Inhibition of FAK decreased tyrosine phosphorylation of p190RhoGAP and elevated the level of GTP-bound Rho. This suggests that FAK regulates cell-cell contact formation by regulation of Rho.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Playford MP,Vadali K,Cai X,Burridge K,Schaller MD

doi

10.1016/j.yexcr.2008.08.010

subject

Has Abstract

pub_date

2008-10-15 00:00:00

pages

3187-97

issue

17

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(08)00331-5

journal_volume

314

pub_type

杂志文章

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