Abstract：
:Zinc is found in a variety of organisms and has been suggested to be essential for many cellular functions. We report here that zinc ions (Zn2+) have a specific and striking effect on cell differentiation in the simple eukaryote, Dictyostelium discoideum. In its asexual reproduction phase, solitary cells gather to form multicellular fruiting bodies consisting of spores and stalk cells. In vitro studies have revealed endogenous factors required for the differentiation of these two cell types. For stalk cell differentiation, these are cyclic AMP and a putative morphogen, a unique chlorinated alkylphenone named differentiation-inducing factor (DIF). With millimolar concentrations of Zn2+, however, cells neglect these requirements altogether and differentiate in vitro into mature stalk cells without the addition of cAMP and/or DIF, expressing stalk-specific markers, wst34 (protein) and ecmB (gene), but not ecmA. Zn2+-induced stalk cell formation is thus distinct from normal stalk cell formation and this system would provide a clue to elucidate the mechanism of stalk cell differentiation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Kubohara Y,Okamoto Kdoi
10.1006/excr.1994.1269subject
Has Abstractpub_date
1994-09-01 00:00:00pages
367-72issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(84)71269-9journal_volume
214pub_type
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1996-11-01 00:00:00
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