Role of YAP/TAZ in cell-matrix adhesion-mediated signalling and mechanotransduction.


:Signalling from the extracellular matrix (ECM) is a fundamental cellular input that sustains proliferation, opposes cell death and regulates differentiation. Through integrins, cells perceive both the chemical composition and physical properties of the ECM. In particular, cell behaviour is profoundly influenced by the mechanical elasticity or stiffness of the ECM, which regulates the ability of cells to develop forces through their contractile actomyosin cytoskeleton and to mature focal adhesions. This mechanosensing ability affects fundamental cellular functions, such that alterations of ECM stiffness is nowadays considered not a simple consequence of pathology, but a causative input driving aberrant cell behaviours. We here discuss recent advances on how mechanical signals intersect nuclear transcription and in particular the activity of YAP/TAZ transcriptional coactivators, known downstream transducers of the Hippo pathway and important effectors of ECM mechanical cues.


Exp Cell Res


Dupont S




Has Abstract


2016-04-10 00:00:00














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    abstract::The beta 1-integrin subunit localizes to focal contacts during F9 teratocarcinoma stem cell differentiation to parietal endoderm. Concomitantly, this integrin subunit becomes dephosphorylated at serine 790, the only serine in the well-conserved cytoplasmic domain of beta 1. We hypothesized that this dephosphorylation ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Barreuther MF,Grabel LB

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  • Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.

    abstract::A novel protein that associates with interphase nucleus and mitotic apparatus (INMAP) was identified by screening HeLa cDNA expression library with an autoimmune serum followed by tandem mass spectrometry. Its complete cDNA sequence of 1.818 kb encodes 343 amino acids with predicted molecular mass of 38.2 kDa and nume...

    journal_title:Experimental cell research

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    authors: Shen E,Lei Y,Liu Q,Zheng Y,Song C,Marc J,Wang Y,Sun L,Liang Q

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  • Sodium 4-phenylbutyrate upregulates ENaC and sodium absorption in T84 cells.

    abstract::Butyrate and other short-chain fatty acids (SCFA), produced by colonic bacterial flora, affect numerous epithelial cell functions. To better understand how SCFA regulate ion transport, we investigated the effects of 4-phenylbutyrate (4-PBA) on Na(+) absorption in T84 cells. Under standard cell culture conditions, the ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Iordache C,Duszyk M

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  • Complement 5a stimulates macrophage polarization and contributes to tumor metastases of colon cancer.

    abstract::Inflammatory cells such as macrophages can play a pro-tumorigenic role in the tumor stroma. Tumor-associated macrophages (TAMs) generally display an M2 phenotype with tumor-promoting activity; however, the mechanisms regulating the TAM phenotype remain unclear. Complement 5a (C5a) is a cytokine-like polypeptide that i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Piao C,Zhang WM,Li TT,Zhang CC,Qiu S,Liu Y,Liu S,Jin M,Jia LX,Song WC,Du J

    更新日期:2018-05-15 00:00:00

  • Colony-stimulating factor 1 (CSF-1) is involved in an autocrine growth control of rat myogenic cells.

    abstract::Colony stimulating factor-1 (CSF-1) and the CSF-1 receptor (the c-fms proto-oncogene product) are expressed during the proliferation of the L6 alpha 1 rat myogenic cell line and both are down-regulated during the differentiation to myotubes. Biologically active CSF-1 was shown to be secreted into the culture medium by...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Borycki AG,Smadja F,Stanley R,Leibovitch SA

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  • Intracellular localization of secretable cAMP in relaying Dictyostelium discoideum cells.

    abstract::Dictyostelium discoideum cells synthesize and secrete the chemoattractant cAMP within minutes after chemotactic stimulation. During development, this signal-relay process is instrumental in cell aggregation, pattern formation, and differentiation. Cyclic AMP is known to accumulate inside the cell before secretion. In ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schoen CD,Arents JC,Bruin T,Van Driel R

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  • Compartmentalization of Fas and Fas ligand may prevent auto- or paracrine apoptosis in epithelial cells.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tan KH,Hunziker W

    更新日期:2003-04-01 00:00:00

  • Induction of apoptosis in human replicative senescent fibroblasts.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: DeJesus V,Rios I,Davis C,Chen Y,Calhoun D,Zakeri Z,Hubbard K

    更新日期:2002-03-10 00:00:00

  • Cytoskeletal association of muscle-specific mRNAs in differentiating L6 rat myoblasts.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bagchi T,Larson DE,Sells BH

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  • Regulation of ROCKII membrane localization through its C-terminus.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kher SS,Worthylake RA

    更新日期:2011-12-10 00:00:00

  • Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7 beta, in metaphase to anaphase transition during embryonic development.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sassa T,Ueda-Ohba H,Kitamura K,Harada S,Hosono R

    更新日期:2003-07-15 00:00:00

  • The effect of colchicine on synaptonemal complex formation in Allium ursinum.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Loidl J

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  • Conventional protein kinase C mediates phorbol-dibutyrate-induced cytoskeletal remodeling in a7r5 smooth muscle cells.

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    pub_type: 杂志文章


    authors: Hai CM,Hahne P,Harrington EO,Gimona M

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    pub_type: 杂志文章


    authors: Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

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  • Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells.

    abstract::Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription wa...

    journal_title:Experimental cell research

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    authors: Egyhazi E,Pigon A,Chang JH,Ghaffari SH,Dreesen TD,Wellman SE,Case ST,Olson MO

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  • Intracellular distribution of poly(ADP-ribose) synthetase in rat spermatogenic cells.

    abstract::The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemic...

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    pub_type: 杂志文章


    authors: Concha II,Figueroa J,Concha MI,Ueda K,Burzio LO

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  • Isolation and characterization of cell cycle-enriched subpopulations of a murine macrophage cell line by centrifugal elutriation.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gandour DM,Walker WS

    更新日期:1983-02-01 00:00:00

  • Promiscuous initiation on mammalian chromosomal DNA templates and its possible suppression by transcription.

    abstract::The Chinese hamster dihydrofolate reductase (DHFR) origin consists of many inefficient initiation sites scattered throughout a 55-kb intergenic spacer, with the ori-beta, ori-beta', and ori-gamma subregions being preferred. To test for the presence of genetic replicators within these subregions, fragments containing o...

    journal_title:Experimental cell research

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    authors: Lin HB,Dijkwel PA,Hamlin JL

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  • Mononuclear cell-mediated modulation of synovial cell metabolism. I. Collagen synthesis.

    abstract::Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pujol JP,Penfornis H,Arenzana-Seisdedos F,Bocquet J,Farjanel J,Rattner A,Brisset M,Virelizier JL,Beliard R,Loyau G

    更新日期:1985-05-01 00:00:00

  • Occurrence of proteinaceous 10-nm filaments throughout the cytoplasm of algae of the order Dasycladales.

    abstract::Previously, whole-mount electron microscopy of nuclei extruded together with residual cytoplasm from the rhizoids of several algal species of the order Dasycladales has revealed the occurrence of an intra- and perinuclear network of 10-nm filaments morphologically indistinguishable from that of mammalian vimentin inte...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Berger S,Wittke W,Traub P

    更新日期:1998-05-01 00:00:00

  • Differential localization of unconventional myosin I and nonmuscle myosin II during B cell spreading.

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    pub_type: 杂志文章


    authors: Sumoza-Toledo A,Gillespie PG,Romero-Ramirez H,Ferreira-Ishikawa HC,Larson RE,Santos-Argumedo L

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  • Hypomethylation of human heterochromatin detected by restriction enzyme nick translation.

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    journal_title:Experimental cell research

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    authors: Mitchell AR

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  • Levels of synthesis of primate-specific nuclear proteins differ between growth-arrested and proliferating cells.

    abstract::A monoclonal antibody that reacts specifically with the proliferation-sensitive nuclear proteins, isoelectric focusing (IEF) 8Z30 and 8Z31 (molecular weight (MW), 76,000 charge variants, HeLa protein catalogue number) has been characterized. As determined by indirect immunofluorescence, the antibody stains the nucleol...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Celis JE,Madsen P,Nielsen S,Petersen Ratz G,Lauridsen JB,Celis A

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  • Active RB elicits late G1/S inhibition.

    abstract::The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Angus SP,Fribourg AF,Markey MP,Williams SL,Horn HF,DeGregori J,Kowalik TF,Fukasawa K,Knudsen ES

    更新日期:2002-06-10 00:00:00

  • Hydrogen peroxide lethality is associated with a decreased ability to maintain positive DNA supercoiling.

    abstract::Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Vaughan AT,Gordon DJ

    更新日期:1992-10-01 00:00:00

  • Immortalization of rat keratinocytes by transfection with polyomavirus large T gene.

    abstract::Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate w...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bayley SA,Stones AJ,Smith CG

    更新日期:1988-07-01 00:00:00

  • p120 serine and threonine phosphorylation is controlled by multiple ligand-receptor pathways but not cadherin ligation.

    abstract::p120-catenin (p120) regulates cadherin turnover and is required for cadherin stability. Extensive and dynamic phosphorylation on tyrosine, serine and threonine residues in the N-terminal regulatory domain has been postulated to regulate p120 function, possibly through modulation of the efficiency of p120/cadherin inte...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Xia X,Carnahan RH,Vaughan MH,Wildenberg GA,Reynolds AB

    更新日期:2006-10-15 00:00:00

  • In vitro differentiation of bone and hypertrophic cartilage from periosteal-derived cells.

    abstract::Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nakahara H,Dennis JE,Bruder SP,Haynesworth SE,Lennon DP,Caplan AI

    更新日期:1991-08-01 00:00:00

  • Epigenetic specification of centromeres by CENP-A.

    abstract::Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Bernad R,Sánchez P,Losada A

    更新日期:2009-11-15 00:00:00

  • Immunological visualization of 5-methylcytosine-rich regions in Indian Muntjac metaphase chromosomes.

    abstract::Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antib...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Vasilikaki-Baker H,Nishioka Y

    更新日期:1983-08-01 00:00:00