Abstract：
:Signalling from the extracellular matrix (ECM) is a fundamental cellular input that sustains proliferation, opposes cell death and regulates differentiation. Through integrins, cells perceive both the chemical composition and physical properties of the ECM. In particular, cell behaviour is profoundly influenced by the mechanical elasticity or stiffness of the ECM, which regulates the ability of cells to develop forces through their contractile actomyosin cytoskeleton and to mature focal adhesions. This mechanosensing ability affects fundamental cellular functions, such that alterations of ECM stiffness is nowadays considered not a simple consequence of pathology, but a causative input driving aberrant cell behaviours. We here discuss recent advances on how mechanical signals intersect nuclear transcription and in particular the activity of YAP/TAZ transcriptional coactivators, known downstream transducers of the Hippo pathway and important effectors of ECM mechanical cues.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Dupont Sdoi
10.1016/j.yexcr.2015.10.034subject
Has Abstractpub_date
2016-04-10 00:00:00pages
42-53issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(15)30139-7journal_volume
343pub_type
杂志文章,评审abstract：:The beta 1-integrin subunit localizes to focal contacts during F9 teratocarcinoma stem cell differentiation to parietal endoderm. Concomitantly, this integrin subunit becomes dephosphorylated at serine 790, the only serine in the well-conserved cytoplasmic domain of beta 1. We hypothesized that this dephosphorylation ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0002
更新日期：1996-01-10 00:00:00
abstract：:A novel protein that associates with interphase nucleus and mitotic apparatus (INMAP) was identified by screening HeLa cDNA expression library with an autoimmune serum followed by tandem mass spectrometry. Its complete cDNA sequence of 1.818 kb encodes 343 amino acids with predicted molecular mass of 38.2 kDa and nume...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.01.022
更新日期：2009-04-15 00:00:00
abstract：:Butyrate and other short-chain fatty acids (SCFA), produced by colonic bacterial flora, affect numerous epithelial cell functions. To better understand how SCFA regulate ion transport, we investigated the effects of 4-phenylbutyrate (4-PBA) on Na(+) absorption in T84 cells. Under standard cell culture conditions, the ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.10.012
更新日期：2007-01-15 00:00:00
abstract：:Inflammatory cells such as macrophages can play a pro-tumorigenic role in the tumor stroma. Tumor-associated macrophages (TAMs) generally display an M2 phenotype with tumor-promoting activity; however, the mechanisms regulating the TAM phenotype remain unclear. Complement 5a (C5a) is a cytokine-like polypeptide that i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.03.009
更新日期：2018-05-15 00:00:00
abstract：:Colony stimulating factor-1 (CSF-1) and the CSF-1 receptor (the c-fms proto-oncogene product) are expressed during the proliferation of the L6 alpha 1 rat myogenic cell line and both are down-regulated during the differentiation to myotubes. Biologically active CSF-1 was shown to be secreted into the culture medium by...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1149
更新日期：1995-05-01 00:00:00
abstract：:Dictyostelium discoideum cells synthesize and secrete the chemoattractant cAMP within minutes after chemotactic stimulation. During development, this signal-relay process is instrumental in cell aggregation, pattern formation, and differentiation. Cyclic AMP is known to accumulate inside the cell before secretion. In ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90181-x
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abstract：:Oligomerization of Fas receptor by its ligand, FasL, activates a signaling cascade that leads to apoptosis of Fas bearing cells. Interestingly, many epithelia coexpress Fas and FasL, yet FasL does not trigger Fas present on the same or neighboring cells to induce spontaneous apoptosis. Here, we show that Fas and FasL ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(02)00056-3
更新日期：2003-04-01 00:00:00
abstract：:Cellular senescence is an irreversible growth phase characteristic of normal cells. We have found that human senescent fibroblasts can be induced to undergo programmed cell death (apoptosis) by ceramide, TNF-alpha, or okadaic acid. The most profound effects were induced by TNF-alpha and okadaic acid treatment. In the ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5425
更新日期：2002-03-10 00:00:00
abstract：:The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts and myotubes were analysed for the presence of ribosomes and mRNPs. Polysomal mRNPs were predominantly associated wit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90425-3
更新日期：1987-01-01 00:00:00
abstract：:RhoA activated kinases (ROCKs) are potent effectors of RhoA signaling for regulation of the cytoskeleton. ROCKs have been shown to be localized to several different subcellular locations, suggesting that its localization is context specific and regulated. However, the signaling mechanisms that control ROCK localizatio...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.09.009
更新日期：2011-12-10 00:00:00
abstract：:In Caenorhabditis elegans embryogenesis, phosphorylation events are critical to chromosomal changes. To investigate the dephosphorylation of chromosome behavior, we cloned and characterized the cDNA that encodes C. elegans protein phosphatase type 1 (CeGLC-7 beta), which is composed of 333 amino acids. CeGLC-7 beta po...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00157-5
更新日期：2003-07-15 00:00:00
abstract：:Interference of colchicine with meiotic chromosome pairing in the wild garlic, Allium ursinum, was studied using a whole-mount spreading technique for synaptonemal complexes. Colchicine was found to cause (i) pairing suppression (arrest of leptotene) and (ii) deficient pairing initiation at zygotene in connection with...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90381-3
更新日期：1988-09-01 00:00:00
abstract：:Phorbol dibutyrate (PDBu) induced the formation of podosome-like structures together with partial disassembly of actin stress fibers in A7r5 smooth muscle cells. These podosomes contained alpha-actinin, F-actin, and vinculin and exhibit a tubular, column-like structure arising perpendicularly from the bottom of PDBu-t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5592
更新日期：2002-10-15 00:00:00
abstract：:The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90312-x
更新日期：1990-02-01 00:00:00
abstract：:Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription wa...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90397-7
更新日期：1988-10-01 00:00:00
abstract：:The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemic...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90063-3
更新日期：1989-02-01 00:00:00
abstract：:The cell cycle of the P388D 1 murine macrophage line was delineated and suspensions of exponentially growing cells were separated by centrifugal elutriation into subpopulations enriched in the various phases of the cycle. Analysis of both growth and labelled mitoses curves disclosed that the doubling and cell-cycle ti...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90058-7
更新日期：1983-02-01 00:00:00
abstract：:The Chinese hamster dihydrofolate reductase (DHFR) origin consists of many inefficient initiation sites scattered throughout a 55-kb intergenic spacer, with the ori-beta, ori-beta', and ori-gamma subregions being preferred. To test for the presence of genetic replicators within these subregions, fragments containing o...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.04.012
更新日期：2005-08-01 00:00:00
abstract：:Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90431-8
更新日期：1985-05-01 00:00:00
abstract：:Previously, whole-mount electron microscopy of nuclei extruded together with residual cytoplasm from the rhizoids of several algal species of the order Dasycladales has revealed the occurrence of an intra- and perinuclear network of 10-nm filaments morphologically indistinguishable from that of mammalian vimentin inte...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3924
更新日期：1998-05-01 00:00:00
abstract：:Cross-linking of CD44 in vitro promotes chemokinesis and actin-based dendrite formation in T and B cells. However, the mechanisms by which the adhesion molecule CD44 induces cytoskeleton activation in lymphocytes are still poorly understood. In this study, we have investigated whether myosin isoforms are involved in C...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.002
更新日期：2006-10-15 00:00:00
abstract：:Using the restriction enzymes MspI and HpaII in the nick translation procedure it has been shown that decondensation of the paracentric heterochromatin of chromosome 9 during human spermatogenesis is associated with hypomethylation of the DNA sequences in this domain. Somatic cells treated with 5'-azacytidine also sho...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90421-4
更新日期：1992-09-01 00:00:00
abstract：:A monoclonal antibody that reacts specifically with the proliferation-sensitive nuclear proteins, isoelectric focusing (IEF) 8Z30 and 8Z31 (molecular weight (MW), 76,000 charge variants, HeLa protein catalogue number) has been characterized. As determined by indirect immunofluorescence, the antibody stains the nucleol...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90011-5
更新日期：1987-02-01 00:00:00
abstract：:The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5510
更新日期：2002-06-10 00:00:00
abstract：:Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90088-p
更新日期：1992-10-01 00:00:00
abstract：:Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate w...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90042-0
更新日期：1988-07-01 00:00:00
abstract：:p120-catenin (p120) regulates cadherin turnover and is required for cadherin stability. Extensive and dynamic phosphorylation on tyrosine, serine and threonine residues in the N-terminal regulatory domain has been postulated to regulate p120 function, possibly through modulation of the efficiency of p120/cadherin inte...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.007
更新日期：2006-10-15 00:00:00
abstract：:Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90401-f
更新日期：1991-08-01 00:00:00
abstract：:Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2009.07.023
更新日期：2009-11-15 00:00:00
abstract：:Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antib...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90289-6
更新日期：1983-08-01 00:00:00
