Abstract：
:Plasminogen activation is observed in the human epidermis during reepithelialization of epidermal defects and under certain pathological conditions. The activation reaction depends on keratinocyte-associated plasminogen activators (PAs), which convert the ubiquitous proenzyme plasminogen into the active trypsin-like serine proteinase plasmin. The PAs are controlled by PA inhibitors (PAIs), of which two major types are known: PAI-1 and PAI-2. In vitro and in vivo keratinocytes express both PAIs. In the current study, we have addressed the possible function of PAI-2 in regulating extracellular PA activity in cultured normal human epidermal keratinocytes (NHEK), the human keratinocyte cell line (HaCaT), and a Ha-ras transfected HaCaT variant (HaRas). PAI-2 was detected intracellularly in all three cell types. Whereas only the NHEK and the HaCaT cells secreted detectable levels of PAI-2 into the culture medium, all three cell types released urokinase-type PA (uPA) into the supernatants. When comparing HaCaT and HaRas cells, we found that the cell lines secreted comparable levels of uPA antigen, whereas the levels of uPA activity were low in the presence of PAI-2, indicating that PAI-2 serves to regulate uPA activity. This assumption was supported by the findings that PAI-2 formed complexes with secreted uPA and that uPA/PAI-2 complexes were present at the surface of the PAI-2-secreting HaCaT cells but not at the surface of PAI-2 nonsecreting HaRas cells. Finally, PAI-2 was found to counteract the uPA-dependent and plasmin-mediated detachment of cultured HaCaT cells. Taken together, our findings indicate that secreted PAI-2 serves to regulate the activity of extracellular uPA in keratinocytes.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Reinartz J,Schaefer B,Bechtel MJ,Kramer MDdoi
10.1006/excr.1996.0061subject
Has Abstractpub_date
1996-02-25 00:00:00pages
91-101issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(96)90061-0journal_volume
223pub_type
杂志文章abstract：:Bax, a multi-domain pro-apoptotic Bcl-2 family member, is a key regulator for the release of apoptogenic factors from mitochondria. MOAP-1, which was first isolated from a screen for Bax-associating proteins, interacts with Bax upon apoptotic induction. MOAP-1 is a short-lived protein that is constitutively degraded b...
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abstract：:Skeletal muscle differentiation is strongly inhibited by transforming growth factor type beta (TGF-beta), although muscle formation as well as regeneration normally occurs in an environment rich in this growth factor. In this study, we evaluated the role of intracellular regulatory Smads proteins as well as TGF-beta-r...
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1996-12-15 00:00:00
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doi：10.1016/j.yexcr.2006.06.029
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doi：10.1016/0014-4827(92)90437-d
更新日期：1992-04-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/0014-4827(92)90294-i
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pub_type： 杂志文章
doi：10.1016/0014-4827(86)90543-4
更新日期：1986-07-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2020-06-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2009.04.016
更新日期：2009-08-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2008.10.026
更新日期：2009-05-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4156
更新日期：1998-10-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4518
更新日期：1999-08-01 00:00:00
abstract：:The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell line...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90126-f
更新日期：1991-05-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1164
更新日期：1995-05-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1006/excr.1996.0362
更新日期：1996-12-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2019-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90531-8
更新日期：1986-07-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90320-5
更新日期：1988-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.02.028
更新日期：2010-07-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2011.03.002
更新日期：2011-05-15 00:00:00
abstract：:The effect of gestational age on serum-mediated changes in enolase activity was tested in a fetal rat brain cell culture. After 6 days exposure to graded concentrations (10 and 20%) of nonpregnant female rat sera, enolase activity in brain cell cultures increased from 2.83 +/- 0.03 to 3.74 +/- 0.19 mumol/min/mg protei...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90298-o
更新日期：1990-02-01 00:00:00
abstract：:Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90105-4
更新日期：1991-04-01 00:00:00
