Activation of ROCK by RhoA is regulated by cell adhesion, shape, and cytoskeletal tension.


:Adhesion to the extracellular matrix regulates numerous changes in the actin cytoskeleton by regulating the activity of the Rho family of small GTPases. Here, we report that adhesion and the associated changes in cell shape and cytoskeletal tension are all required for GTP-bound RhoA to activate its downstream effector, ROCK. Using an in vitro kinase assay for endogenous ROCK, we found that cells in suspension, attached on substrates coated with low density fibronectin, or on spreading-restrictive micropatterned islands all exhibited low ROCK activity and correspondingly low myosin light chain phosphorylation, in the face of high levels of GTP-bound RhoA. In contrast, allowing cells to spread against substrates rescued ROCK and myosin activity. Interestingly, inhibition of tension with cytochalasin D or blebbistatin also inhibited ROCK activity within 20 min. The abrogation of ROCK activity by cell detachment or inhibition of tension could not be rescued by constitutively active RhoA-V14. These results suggest the existence of a feedback loop between cytoskeletal tension, adhesion maturation, and ROCK signaling that likely contributes to numerous mechanochemical processes.


Exp Cell Res


Bhadriraju K,Yang M,Alom Ruiz S,Pirone D,Tan J,Chen CS




Has Abstract


2007-10-01 00:00:00














  • Mitosis-arresting effect of the calcium channel inhibitor SK&F 96365 on human leukemia cells.

    abstract::The effect of SK&F 96365 (1-(beta-[3-(4-methoxyphenyl)propoxyl]-4- methoxyphenethyl)-1H-imidazole hydrochloride), a recently synthesized inhibitor of receptor-mediated calcium entry, was investigated on human hematopoietic cell lines. We found that treatment of the T-cell leukemia line Jurkat with SK&F 96365 inhibited...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nordström T,Nevanlinna HA,Andersson LC

    更新日期:1992-10-01 00:00:00

  • Conditional inactivation of Brca1 in the mouse ovarian surface epithelium results in an increase in preneoplastic changes.

    abstract::Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Clark-Knowles KV,Garson K,Jonkers J,Vanderhyden BC

    更新日期:2007-01-01 00:00:00

  • Dynamic expression of the cell adhesion molecule cell-CAM 105 in fetal and regenerating rat liver.

    abstract::Cell-CAM 105 is an integral cell surface glycoprotein that is involved in cell-cell adhesion of adult rat hepatocytes in vitro. In the present report we used a radio-immunoassay, a quantitative immunoblotting technique and immunofluorescence microscopy to investigate the expression of cell-CAM 105 in fetal and regener...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Odin P,Obrink B

    更新日期:1986-05-01 00:00:00

  • Trichostatin A affects the secretion pathways of beta and intestinal endocrine cells.

    abstract::Histone deacetylase inhibitors (HDACi) were recently identified as having significant clinical potential in reversing β-cell functional inhibition caused by inflammation, a shared precursor of Type 1 and Type 2 diabetes. However, HDACi are highly complex and little is known of their direct effect on important cell sec...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tiernan AR,Champion JA,Sambanis A

    更新日期:2015-01-01 00:00:00

  • miR-133b suppresses colorectal cancer cell stemness and chemoresistance by targeting methyltransferase DOT1L.

    abstract::Cancer stem cells (CSCs) are a subpopulation of chemoresistant cells that play a critical role in disease recurrence following chemotherapy. It has been reported that microRNA-133b (miR-133b) acts as a tumor suppressor in colorectal cancer (CRC). However, whether miR-133b is associated with CRC stemness and chemoresis...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lv L,Li Q,Chen S,Zhang X,Tao X,Tang X,Wang S,Che G,Yu Y,He L

    更新日期:2019-12-01 00:00:00

  • Commitment of 3T3-F442A cells to adipocyte differentiation takes place during the first 24-36 h after adipogenic stimulation: TNF-alpha inhibits commitment.

    abstract::We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Castro-Muñozledo F,Beltrán-Langarica A,Kuri-Harcuch W

    更新日期:2003-04-01 00:00:00

  • 1,25-Dihydroxyvitamin D3 and Transforming Growth Factor-beta Act Synergistically to Override Extinction of LiversolidusBonesolidusKidney Alkaline Phosphatase in Osteosarcoma Hybrid Cells

    abstract::In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Johnson-Pais TL,Leach RJ

    更新日期:1996-07-10 00:00:00

  • Extracellular ATP mobilizes intracellular Ca2+ in T51B rat liver epithelial cells: a study involving single cell measurements.

    abstract::T51B rat liver epithelial cells were stimulated with extracellular ATP. Changes in cytoplasmic free Ca2+ concentration [( Ca2+]i) were measured by fura-2 both in a large population of cells on coverslips in a cuvette and in single cells in a microscopic system. Extracellular ATP evoked a prompt increase in [Ca2+]i in ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Boynton AL,Cooney RV,Hill TD,Nilsson T,Arkhammar P,Berggren PO

    更新日期:1989-03-01 00:00:00

  • Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors.

    abstract::Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/we...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Oikawa Y,Hansson J,Sasaki T,Rousselle P,Domogatskaya A,Rodin S,Tryggvason K,Patarroyo M

    更新日期:2011-05-01 00:00:00

  • External Ca2+ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells.

    abstract::Polycystin-2 (PC2, TRPP2) is a nonselective cation channel whose dysfunction is associated with the onset of autosomal dominant polycystic kidney disease (ADPKD). PC2 contributes to Ca2+ transport and cell signaling in renal epithelia and other tissues. Little is known however, as to the external Ca2+ regulation of PC...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Dai XQ,Perez PL,Soria G,Scarinci N,Smoler M,Morsucci DC,Suzuki K,Cantero MDR,Cantiello HF

    更新日期:2017-01-01 00:00:00

  • ERα inhibited myocardin-induced differentiation in uterine fibroids.

    abstract::Uterine fibroids, also known as uterine leiomyomas, are a benign tumor of the human uterus and the commonest estrogen-dependent benign tumor found in women. Myocardin is an important transcriptional regulator in smooth and cardiac muscle development. The role of myocardin and its relationship with ERα in uterine fibro...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liao XH,Li JY,Dong XM,Wang X,Xiang Y,Li H,Yu CX,Li JP,Yuan BY,Zhou J,Zhang TC

    更新日期:2017-01-01 00:00:00

  • SNEV overexpression extends the life span of human endothelial cells.

    abstract::In a recent screening for genes down regulated in replicatively senescent human umbilical vein endothelial cells (HUVECs), we have isolated the novel protein SNEV. Since then SNEV has proven as a multifaceted protein playing a role in pre-mRNA splicing, DNA repair, and the ubiquitin/proteosome system. Here, we report ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Voglauer R,Chang MW,Dampier B,Wieser M,Baumann K,Sterovsky T,Schreiber M,Katinger H,Grillari J

    更新日期:2006-04-01 00:00:00

  • Repopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro. A novel system for type II pneumocyte culture.

    abstract::This paper describes the preparation of lung acellular alveolar matrix fragments and culture of rat type II pneumocytes directly on the alveolar epithelial basement membrane, thereby permitting study of the effect of lung basement membrane on the morphology and function of type II cells. Collagen types I, III, IV and ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lwebuga-Mukasa JS,Ingbar DH,Madri JA

    更新日期:1986-02-01 00:00:00

  • Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.

    abstract::Since the migration of human fetal skin fibroblasts into a denuded area of a cell monolayer has been demonstrated to be serum-independent (fetal phenotype), whereas that of adult-donor skin fibroblasts is serum-dependent (adult phenotype), a study was carried out to determine at which life stage the fetal-adult phenot...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kondo H,Yonezawa Y

    更新日期:1995-10-01 00:00:00

  • Phorbol ester- and calcium-induced reorganization of 180-kDa bullous pemphigoid antigen on the ventral surface of cultured human keratinocytes as studied by immunofluorescence and immunoelectron microscopy.

    abstract::The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kitajima Y,Owaribe K,Nishizawa Y,Jokura Y,Yaoita H

    更新日期:1992-11-01 00:00:00

  • Ultrastructural distribution of the death-domain-containing MyD88 protein in HeLa cells.

    abstract::MyD88, a protein implicated in interleukin-1 signaling, was localized in HeLa cells transiently transfected with an epitope-tagged (flag) version of MyD88. Overexpression of MyD88 can induce apoptosis. We have analyzed the fine structural intracellular distribution of MyD88 using immunoelectron microscopy. MyD88 is lo...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jaunin F,Burns K,Tschopp J,Martin TE,Fakan S

    更新日期:1998-08-25 00:00:00

  • IgG binding to cytoskeletal intermediate filaments activates the complement cascade.

    abstract::The cellular plasma membrane becomes permeable to macromolecules during the cell injury process. This results in exposure of the interior of the cell to plasma proteins and to high-affinity binding of the Fc part of IgG to intermediate filaments (Hansson, G K, Starkebaum, G A, Benditt, E P & Schwartz, S M, Proc natl a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hansson GK,Lagerstedt E,Bengtsson A,Heideman M

    更新日期:1987-06-01 00:00:00

  • Interleukin-2 induces a rapid increase in ornithine decarboxylase mRNA in a cloned murine T lymphocytic cell line.

    abstract::We recently observed a 25-fold increase in the activity of ornithine decarboxylase (ODC) 6 h after treatment of G1-synchronized CTLL-2 cells with interleukin-2 (IL-2). Here we show that the ODC mRNA content increased in parallel with the ODC activity during the first hours of stimulation with IL-2, resulting in a 25-f...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Legraverend C,Potter A,Hölttä E,Alitalo K,Andersson LC

    更新日期:1989-03-01 00:00:00

  • 6-Dimethylaminopurine blocks starfish oocyte maturation by inhibiting a relevant protein kinase activity.

    abstract::The puromycin analog N6,N6-dimethyladenine (6-dimethylaminopurine or 6-DMAP) was found to inhibit meiosis reinitiation in starfish oocytes stimulated by the natural hormone 1-methyladenine. Increasing concentrations of this agent delayed and eventually blocked germinal vesicle breakdown. They were found to be effectiv...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Neant I,Guerrier P

    更新日期:1988-05-01 00:00:00

  • Alpha-factor inhibition of the rate of cell passage through the "start" step of cell division in Saccharomyces cerevisiae yeast: estimation of the division delay per alpha-factor.receptor complex.

    abstract::A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Moore SA

    更新日期:1987-08-01 00:00:00

  • miR-125b/Ets1 axis regulates transdifferentiation and calcification of vascular smooth muscle cells in a high-phosphate environment.

    abstract:OBJECTIVES:Vascular calcification is highly prevalent in patients with chronic kidney disease (CKD) and contributes to increased risk of cardiovascular disease and mortality. Accumulated evidences suggested that vascular smooth muscle cells (VSMCs) to osteoblast-like cells transdifferentiation (VOT) plays a crucial rol...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wen P,Cao H,Fang L,Ye H,Zhou Y,Jiang L,Su W,Xu H,He W,Dai C,Yang J

    更新日期:2014-04-01 00:00:00

  • Changes in deoxyribonucleoside triphosphate pools in the starfish oocyte during maturation and early embryogenesis.

    abstract::dNTP pools are quite low in immature oocytes of the starfish, expand during the 1-methyladenine-induced maturational process and thereafter reach a maximal level (approx. 35, 20, 15 and 5 fmoles/egg for dTTP, dCTP, dATP and dGTP, respectively) which is maintained in overmatured eggs. Maturing oocytes were inseminated ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nagano H,Okano K,Ikegami S

    更新日期:1983-04-15 00:00:00

  • Phenotype-dependent expression of alpha-smooth muscle actin in visceral smooth muscle cells.

    abstract::Alpha-Smooth muscle actin is one of the molecular markers for a phenotype of vascular smooth muscle cells, because the actin is a major isoform expressed in vascular smooth muscle cells and its expression is upregulated during differentiation. Here, we first demonstrate that the phenotype-dependent expression of this ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Saga H,Kimura K,Hayashi K,Gotow T,Uchiyama Y,Momiyama T,Tadokoro S,Kawashima N,Jimbou A,Sobue K

    更新日期:1999-02-25 00:00:00

  • Multidrug-resistance phenotype of a subpopulation of T-lymphocytes without drug selection.

    abstract::Multidrug-resistant (MDR) cells demonstrate the increased activity of the membrane transport system performing efflux of diverse lipophylic drugs and fluorescent dyes from the cells. In order to detect MDR cells we have developed a simple test consisting of three steps: staining of the cells with fluorescent dye rhoda...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Neyfakh AA,Serpinskaya AS,Chervonsky AV,Apasov SG,Kazarov AR

    更新日期:1989-12-01 00:00:00

  • Construction of transferrin-coated liposomes for in vivo transport of exogenous DNA to bone marrow erythroblasts in rabbits.

    abstract::A method for coating liposomes with transferrin is described. Such liposomes have been used for carrying exogenous DNA to rabbit bone marrow (BM) precursor cells, in vivo. A large amount of the DNA contained in the liposomes was delivered specifically into the erythroblasts of the test animals. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Stavridis JC,Deliconstantinos G,Psallidopoulos MC,Armenakas NA,Hadjiminas DJ,Hadjiminas J

    更新日期:1986-06-01 00:00:00

  • Caloric restriction ameliorates cardiomyopathy in animal model of diabetes.

    abstract:BACKGROUND:The db/db mouse is an animal model of diabetes in which leptin receptor activity is deficient resulting accelerated cardiomyopathy when exposed to angiotensin (AT). Toll-like receptors 4 and 2 (TLR4, TLR2) are pattern recognition receptors, that recognize pathogen-associated molecular patterns and exacerbate...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cohen K,Waldman M,Abraham NG,Laniado-Schwartzman M,Gurfield D,Aravot D,Arad M,Hochhauser E

    更新日期:2017-01-01 00:00:00

  • Activity of a novel PDGF beta-receptor enhancer during the cell cycle and upon differentiation of neuroblastoma.

    abstract::PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF beta-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering the entire human PDGFR...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kaneko M,Yang W,Matsumoto Y,Watt F,Funa K

    更新日期:2006-07-01 00:00:00

  • A keratin of fetal skin is reexpressed in human keratinocytes transformed by SV40 virus or treated with the tumor promoter TPA.

    abstract::SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

    更新日期:1984-09-01 00:00:00

  • A comparison of the rate of DNA synthesis in myeloblasts from peripheral blood and bone marrows of patients with acute nonlymphocytic leukemia.

    abstract::Durations of S-phase (Ts) and total cell cycle times (Tc) were measured from the peripheral blood (PB) and bone marrow aspirates (BM) of five patients with acute nonlymphocytic leukemia (ANLL). Intravenous bromodeoxyuridine (BrdU) was used as the first label for S-phase cells and a monoclonal anti-BrdU antibody was us...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Raza A,Yasin Z,Grande C

    更新日期:1988-05-01 00:00:00

  • Mitf regulates osteoclastogenesis by modulating NFATc1 activity.

    abstract::Transcription factors Mitf and NFATc1 share many downstream targets that are critical for osteoclastogenesis. Since RANKL signals induce/activate both NFATc1 and Mitf isoform-E (Mitf-E), a tissue-restricted Mitf isoform in osteoclasts, it is plausible that the two factors work together to promote osteoclastogenesis. A...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lu SY,Li M,Lin YL

    更新日期:2014-10-15 00:00:00