Abstract:
:Incubation of cultured cells at 20 degrees C blocks the transport of newly synthesized plasma membrane proteins, and the proteins accumulate intracellularly in a terminally glycosylated form. When baby hamster kidney cells are infected with the ts O45 mutant of vesicular stomatitis virus, and incubated at 20 degrees C, the terminally glycosylated spike glycoprotein G of the virus accumulates in the membranes of a tubular network localized on the trans side of the Golgi cisternae, the trans-Golgi network (TGN). We have used the G protein of ts O45 as a marker for the TGN and isolated a TGN fraction using a combination of conventional cell fractionation techniques and immunoisolation. The TGN was separated from the bulk of the endoplasmic reticulum, mitochondria, lysosomes, plasma membrane, and endosomes, while the activity of trans-Golgi marker galactosyltransferase copurified with the G protein. Using G protein as the TGN marker we have determined that the TGN was enriched 25-fold in the final fraction relative to the total homogenate. Several polypeptides (Mr 75,000, 87,000, 92,000, and 120,000) copurified with the G protein in the isolated TGN fraction and most likely represent resident markers of the compartment.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
de Curtis I,Howell KE,Simons Kdoi
10.1016/0014-4827(88)90190-5subject
Has Abstractpub_date
1988-04-01 00:00:00pages
248-65issue
2eissn
0014-4827issn
1090-2422journal_volume
175pub_type
杂志文章abstract::Ets variant gene 5 (ETV5) and glial cell-derived neurotrophic factor (GDNF) are produced in Sertoli cells and required for maintenance and self-renewal of spermatogonial stem cells (SSCs) in mice. Fibroblast growth factors (FGFs) have been reported to stimulate Etv5 mRNA expression, and FSH was shown to stimulate Gdnf...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.05.002
更新日期:2007-08-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.07.016
更新日期:2004-12-10 00:00:00
abstract::A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90438-z
更新日期:1991-12-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4104
更新日期:1998-07-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1006/excr.2000.5039
更新日期:2000-11-25 00:00:00
abstract::Dipeptidyl peptidase IV (DPPIV) is a serine peptidase that cleaves N-terminal dipeptides from polypeptides when the second residue is a proline or an alanine. We have recently cloned cDNAs for rat gp110, a membrane glycoprotein with Mr of 110,000 isolated initially from rat liver. Studies reported here establish that ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90296-6
更新日期:1989-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90087-b
更新日期:1991-02-01 00:00:00
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journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2017.08.007
更新日期:2017-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1332
更新日期:1995-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90343-7
更新日期:1992-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2011.01.018
更新日期:2011-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-02-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.03.022
更新日期:2019-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.01.008
更新日期:2018-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.12.016
更新日期:2013-02-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.12.009
更新日期:2007-03-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1210
更新日期:1993-08-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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更新日期:2013-08-15 00:00:00
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pub_type: 杂志文章
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更新日期:2005-10-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1988-04-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90201-2
更新日期:1989-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1999-04-10 00:00:00