Two classes of single-stranded regions evident in deproteinized preparations of replicating DNA isolated from mammalian cells.

abstract：:The existing cytometric methodologies do not allow one to directly correlate, within the same cells, functional cell attributes that are revealed supravitally with features that require cell fixation to be detected or measured. Taking advantage of the "file merge" feature of the laser-scanning cytometer, we have been ...

journal_title：Experimental cell research

authors： Li X,Darzynkiewicz Z

更新日期：1999-06-15 00:00:00

abstract：:Human neutrophils developed long thin tubulovesicular extensions (cytonemes) upon adhesion to fibronectin-coated substrata, when spreading was blocked. We observed extension formation when neutrophils were plated to fibronectin-coated substrata in Na(+)-free extracellular medium or in the presence of drugs capable of ...

journal_title：Experimental cell research

authors： Galkina SI,Sud'ina GF,Ullrich V

更新日期：2001-06-10 00:00:00

abstract：:The centromere is a specialized region of the eukaryotic chromosome that is responsible for directing chromosome movements in mitosis and for coordinating the progression of mitotic events at the crucial transition between metaphase and anaphase. In this review, we will focus on recent advances in the understanding of...

journal_title：Experimental cell research

authors： Craig JM,Earnshaw WC,Vagnarelli P

更新日期：1999-02-01 00:00:00

abstract：:The objective of the present study was to develop an accurate and reproducible method for liver sinusoidal endothelial cell (LSEC) isolation in mice. Non-parenchymal cells were isolated using a modified two-step collagenase digestion combined with Optiprep density gradient centrifugation. LSEC were further purified us...

journal_title：Experimental cell research

authors： Meyer J,Lacotte S,Morel P,Gonelle-Gispert C,Bühler L

更新日期：2016-12-10 00:00:00

abstract：:Fluid-phase endocytosis was significantly faster in three lines of human lysosomal storage disease fibroblasts (two I cells and one Sandhoff's) than in three normals. Re-exocytosis of fluid showed similar kinetics in all lines when release was expressed as a percentage of the radioactivity associated with the cells at...

journal_title：Experimental cell research

authors： Dean RT

更新日期：1984-04-01 00:00:00

abstract：:Autophagy has been shown to be critically associated with the central mechanisms underlying Parkinson's disease (PD), while the mechanisms contributing to the imbalance of autophagy remain unclear. Small nucleolar RNA host gene 1 (SNHG1), a well-studied long noncoding RNA, has been reported to be significantly increas...

journal_title：Experimental cell research

authors： Qian C,Ye Y,Mao H,Yao L,Sun X,Wang B,Zhang H,Xie L,Zhang H,Zhang Y,Zhang S,He X

更新日期：2019-11-01 00:00:00

abstract：:DNA topoisomerase (topo) I plays an important role in DNA metabolism by relieving the torsional restraints of DNA topology through ATP-independent single-strand DNA breakage. In the present study, we expressed human topo I in HeLa cells by fusing it to enhanced green fluorescent protein (EGFP). The EGFP-topo I fusion ...

journal_title：Experimental cell research

authors： Mo YY,Wang P,Beck WT

更新日期：2000-05-01 00:00:00

abstract：:The release of cell surface-derived microparticles, or ectosomes, has now been described for many different cell types. In various diseases characterized by systemic inflammation, the numbers of ectosomes released from specific cell-types are found increased manifold in the circulation. Their pro-inflammatory and pro-...

journal_title：Experimental cell research

authors： Gasser O,Schifferli JA

更新日期：2005-07-15 00:00:00

abstract：:We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. Th...

journal_title：Experimental cell research

authors： Keherly MJ,Papaconstantinou J,McCombs JL

更新日期：1993-04-01 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00

abstract：OBJECTIVES:This study aimed to investigate the expression changes of LOX (lysyl oxidase) family genes, VEGFA, and VEGFB under hypoxic conditions in a co-culture system of ASCs (adipose-derived stromal cells) and ECs (endothelial cells). MATERIALS AND METHODS:ASCs and ECs were co-cultured under hypoxic and normal oxyge...

journal_title：Experimental cell research

authors： Xie Q,Xie J,Tian T,Ma Q,Zhang Q,Zhu B,Cai X

更新日期：2017-03-01 00:00:00

abstract：:Recently, SIRT5 was reported to be a predominant desuccinylase and demalonylase in mitochondria. Ablation of SIRT5 enhances the systemic succinylation and malonylation of mitochondrial proteins, including various metabolic enzymes; however, its function in pancreatic β cells has not yet been clarified. In this study, ...

journal_title：Experimental cell research

authors： Wang Y,Liu Q,Huan Y,Li R,Li C,Sun S,Guo N,Yang M,Liu S,Shen Z

更新日期：2018-10-01 00:00:00

abstract：:Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...

journal_title：Experimental cell research

authors： Gazziola C,Moras M,Ferraro P,Gallinaro L,Verin R,Rampazzo C,Reichard P,Bianchi V

更新日期：1999-12-15 00:00:00

abstract：:Using L1210 murine leukemia cells, we have previously shown that in response to treatment with drugs having different targets, apoptotic cell death occurs through at least two different signaling pathways. Here, we present evidence that nuclear extracts from staurosporine-treated cells elicit DNase II activity that is...

journal_title：Experimental cell research

authors： Belmokhtar CA,Torriglia A,Counis MF,Courtois Y,Jacquemin-Sablon A,Ségal-Bendirdjian E

更新日期：2000-01-10 00:00:00

abstract：:The RAI gene is also known as iASPP and PPP1R13L. Recent investigations have shown that the region encompassing RAI is important for the development of cancer in young and middle-aged persons. It has been speculated that the RAI product induces apoptosis by blocking NF-kappaB or inhibits apoptosis by blocking p53. Eit...

journal_title：Experimental cell research

authors： Laska MJ,Strandbygård D,Kjeldgaard A,Mains M,Corydon TJ,Memon AA,Sørensen BS,Vogel U,Jensen UB,Nexø BA

更新日期：2007-07-15 00:00:00

abstract：:Megakaryocytes (Mks) are unique cells in the human body in that they carry a single and polyploid nucleus. It is therefore of interest to understand their nuclear ultrastructure. PML oncogenic domains (PODs) were described in several types of eukaryotic cells using human autoantibodies which recognize nuclear antigens...

journal_title：Experimental cell research

authors： Drouin A,Schmitt A,Massé JM,Cieutat AM,Fichelson S,Cramer EM

更新日期：2001-12-10 00:00:00

abstract：BACKGROUND AND OBJECTIVE:In recent years, immunotherapy for the treatment of tumors have been established. Dendritic cells (DCs) are extremely efficient and professional antigen presenting cells (APCs), which are an important target for immune therapeutic interventions in cancer. In present study, we investigated wheth...

journal_title：Experimental cell research

authors： Gao X,Han D,Fan W

更新日期：2016-12-10 00:00:00

abstract：:We have used N-hydroxysuccinimido-d-biotin as a reagent for labeling proteins exposed at the surface of cultured bovine adrenal chromaffin cells during Ba2+-stimulated secretion. A specific secretory granule membrane constituent, dopamine-beta-hydroxylase (DBH), has been investigated using immunoprecipitation followed...

journal_title：Experimental cell research

authors： Hunter A,Phillips JH

更新日期：1989-06-01 00:00:00

abstract：:Earlier we reported the undetectable level of endothelin (ET)-1 mRNA in aorta from young donors, in contrast to the detectable levels in older donors (Lab. Invest. 67, 210-217, 1992). We also found that the synthesis of ET-1 peptide is elevated in cultured endothelial cells from the aorta of over-50-year-old donors. I...

journal_title：Experimental cell research

authors： Kumazaki T,Fujii T,Kobayashi M,Mitsui Y

更新日期：1994-03-01 00:00:00

abstract：:By means of plasma clot clonal cultures, the content of the avian spleen in granulomonocytic progenitors was studied during ontogeny. Serum-free media were used that were supplemented with growth activities produced either by embryonic fibroblasts or adult spleen cells. These two conditioned media not only permitted t...

journal_title：Experimental cell research

authors： Nicolas-Bolnet C,Yassine F,Cormier F,Dieterlen-Lièvre F

更新日期：1991-10-01 00:00:00

abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...

journal_title：Experimental cell research

authors： Borrione AC,Zanellato AM,Giuriato L,Scannapieco G,Pauletto P,Sartore S

更新日期：1990-09-01 00:00:00

abstract：:The characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wave-length microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cyt...

journal_title：Experimental cell research

authors： Tsunoda Y

更新日期：1990-06-01 00:00:00

abstract：:Cellular senescence is an irreversible growth phase characteristic of normal cells. We have found that human senescent fibroblasts can be induced to undergo programmed cell death (apoptosis) by ceramide, TNF-alpha, or okadaic acid. The most profound effects were induced by TNF-alpha and okadaic acid treatment. In the ...

journal_title：Experimental cell research

authors： DeJesus V,Rios I,Davis C,Chen Y,Calhoun D,Zakeri Z,Hubbard K

更新日期：2002-03-10 00:00:00

abstract：:Hyperglycemia is a primary factor that disturbs podocyte function in the glomerular filtration process; this disturbance leads to the development of diabetic nephropathy, and ultimately, renal failure. Podocyte function may also be altered by biological agents that modify protein kinase activity, including the cGMP-ac...

journal_title：Experimental cell research

authors： Piwkowska A,Rogacka D,Audzeyenka I,Angielski S,Jankowski M

更新日期：2014-01-01 00:00:00

abstract：:The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...

journal_title：Experimental cell research

authors： Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V

更新日期：2015-04-10 00:00:00

abstract：:The male fetal lung begins to synthesize surfactant later in gestation than the female. This delay appears to be caused by androgens. We hypothesized that male fetal lung differentiation is delayed as a consequence of an extended phase of growth which is elicited by androgens. We observed that in vivo fetal lung prote...

journal_title：Experimental cell research

authors： Nielsen HC,Kirk WO,Sweezey N,Torday JS

更新日期：1990-05-01 00:00:00

abstract：:Several cell division cycle (cdc) mutants of Saccharomyces cerevisiae (cdc28, cdc35, cdc19, cdc21, and cdc17) at the restrictive temperature (37 degrees C) in the presence of 1% glucose and defined medium divert most of the carbon (approximately 50%) to ethanol production with low biomass growth yields (Yglc) that cor...

journal_title：Experimental cell research

authors： Mónaco ME,Valdecantos PA,Aon MA

更新日期：1995-03-01 00:00:00

abstract：:The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase pro...

journal_title：Experimental cell research

authors： Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KH

更新日期：1983-12-01 00:00:00

abstract：:Functional imaging of the intracellular calcium concentration [Ca2+]i using fluorescent indicators is a powerful and frequently applied method for assessing various biological questions in vitro, including ion channel function and intracellular signaling in homeostasis and disease. In functional [Ca2+]i imaging experi...

journal_title：Experimental cell research

authors： Ritter P,Bye LJ,Finol-Urdaneta RK,Lesko C,Adams DJ,Friedrich O,Gilbert DF

更新日期：2020-10-15 00:00:00

abstract：:Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...

journal_title：Experimental cell research

authors： Pujol JP,Penfornis H,Arenzana-Seisdedos F,Bocquet J,Farjanel J,Rattner A,Brisset M,Virelizier JL,Beliard R,Loyau G

更新日期：1985-05-01 00:00:00