Mammalian centromeres: DNA sequence, protein composition, and role in cell cycle progression.

abstract：:The major cell surface proteins of Chinese hamster ovary (CHO) cells have been investigated after reacting cells at 4 degrees C with the membrane-impermeant reagent, trinitrobenzenesulfonate (TNBS). Immunoprecipitation and subsequent two-dimensional, sodium-dodecyl sulfate, polyacrylamide gel electrophoresis (SDS-PAGE...

journal_title：Experimental cell research

authors： Raub TJ,Denny JB,Roberts RM

更新日期：1986-07-01 00:00:00

abstract：:Translational control is an essential process in regulation of gene expression, which occurs at the initiation step performed by a number of translation initiation factor complexes. eIF3a (eIF3 p170) is the largest subunit of the eIF3 complex. eIF3a has been suggested to play roles in regulating translation of a subse...

journal_title：Experimental cell research

authors： Dong Z,Liu Z,Cui P,Pincheira R,Yang Y,Liu J,Zhang JT

更新日期：2009-07-01 00:00:00

abstract：:We examined the relationship of cellular oncogene c-myc and transferrin receptor (TfR) gene expression to cell proliferation and cell cycle progression during myeloid differentiation in the HL-60 myeloid leukemia cell line. In order to determine levels of mRNA for these genes in HL-60 cells induced to differentiate al...

journal_title：Experimental cell research

authors： Barker KA,Newburger PE

更新日期：1990-01-01 00:00:00

abstract：:In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...

journal_title：Experimental cell research

authors： Weitzel G,Pilatus U,Rensing L

更新日期：1987-05-01 00:00:00

abstract：:Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracel...

journal_title：Experimental cell research

authors： Thieszen SL,Dalton M,Gadson PF,Patterson E,Rosenquist TH

更新日期：1996-08-25 00:00:00

abstract：:We have characterized glucocerebrosidase in various cell types of peripheral blood of control subjects and in cultured human blastoid cells. The intracellular level of glucocerebrosidase in cultured blastoid cells (10-30 nmol substrate hydrolyzed/h.mg protein) resembles closely values observed for leukocyte cell types...

journal_title：Experimental cell research

authors： Aerts JM,Heikoop J,van Weely S,Donker-Koopman WE,Barranger JA,Tager JM,Schram AW

更新日期：1988-08-01 00:00:00

abstract：:Cellular senescence is characterized by a decline in sensitivity to growth factors resulting in cessation of cellular growth. The expression of cellular or viral oncogenes may result in the establishment of cell lines with unlimited proliferative potential ("immortalization"). A variety of viral and cellular oncogenes...

journal_title：Experimental cell research

authors： Linder S,Marshall H

更新日期：1990-11-01 00:00:00

abstract：:We developed an improved method for isolation of peroxisome biogenesis-defective somatic animal cell mutants, using a combination of green fluorescent protein (GFP) expression and the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) selection method. We used TKaG1 and TKaG2 cells, the wild-type Chinese hamster ovary (CHO) c...

journal_title：Experimental cell research

authors： Ghaedi K,Kawai A,Okumoto K,Tamura S,Shimozawa N,Suzuki Y,Kondo N,Fujiki Y

更新日期：1999-05-01 00:00:00

abstract：:We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecul...

journal_title：Experimental cell research

authors： Zákány R,Szucs K,Bakó E,Felszeghy S,Czifra G,Bíró T,Módis L,Gergely P

更新日期：2002-04-15 00:00:00

abstract：:Coagulation Factor (F)Xa is a serine protease that plays a crucial role during blood coagulation by converting prothrombin into active thrombin. Recently, however, it emerged that besides this role in coagulation, FXa induces intracellular signaling leading to different cellular effects. Here, we show that coagulation...

journal_title：Experimental cell research

authors： Borensztajn KS,Bijlsma MF,Groot AP,Brüggemann LW,Versteeg HH,Reitsma PH,Peppelenbosch MP,Spek CA

更新日期：2007-07-15 00:00:00

abstract：:Polyamines affect nucleosome oligomerization and DNA conformation in vitro, yet little information exists regarding the influence of naturally synthesized polyamines on mammalian chromatin. Capitalizing on the relative inefficiency of a moderate ionic strength extraction buffer to dissociate histones, we obtained evid...

journal_title：Experimental cell research

authors： Hobbs CA,Paul BA,Gilmour SK

更新日期：2003-11-01 00:00:00

abstract：:To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formalde...

journal_title：Experimental cell research

authors： DeCourcy K,Storrie B

更新日期：1991-01-01 00:00:00

abstract：:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...

journal_title：Experimental cell research

authors： Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L

更新日期：1996-04-10 00:00:00

abstract：:Several species of Streptococcus, such as S. salivarius, S. mitis, and S. anginosus, are found to extensively colonize the oral cavity and the upper respiratory tract, and have been shown to increase in patients with oral squamous cell carcinoma (OSCC). Accumulating evidence have revealed that commensal bacteria are i...

journal_title：Experimental cell research

authors： Wang J,Sun F,Lin X,Li Z,Mao X,Jiang C

更新日期：2018-01-01 00:00:00

abstract：:Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...

journal_title：Experimental cell research

authors： Suzuki ST

更新日期：2000-11-25 00:00:00

abstract：:Paclitaxel (Taxol) has been successfully combined with the monoclonal antibody trastuzumab (Herceptin) in the treatment of ErbB2 overexpressing cancers. However, this combination therapy showed an unexpected synergistic increase in cardiac dysfunction. We have studied the mechanisms of paclitaxel/anti-ErbB2 cardiotoxi...

journal_title：Experimental cell research

authors： Pentassuglia L,Timolati F,Seifriz F,Abudukadier K,Suter TM,Zuppinger C

更新日期：2007-05-01 00:00:00

abstract：:Ets variant gene 5 (ETV5) and glial cell-derived neurotrophic factor (GDNF) are produced in Sertoli cells and required for maintenance and self-renewal of spermatogonial stem cells (SSCs) in mice. Fibroblast growth factors (FGFs) have been reported to stimulate Etv5 mRNA expression, and FSH was shown to stimulate Gdnf...

journal_title：Experimental cell research

authors： Simon L,Ekman GC,Tyagi G,Hess RA,Murphy KM,Cooke PS

更新日期：2007-08-15 00:00:00

abstract：:Mitochondrial DNA depleted (ρ(0)) human skin fibroblasts (HSF) with suppressed oxidative phosphorylation were characterized by significant changes in the expression of 2100 nuclear genes, encoding numerous protein classes, in NF-κB and STAT3 signaling pathways, and by decreased activity of mitochondrial death pathway,...

journal_title：Experimental cell research

authors： Ivanov VN,Ghandhi SA,Zhou H,Huang SX,Chai Y,Amundson SA,Hei TK

更新日期：2011-07-01 00:00:00

abstract：:The differential activation of cyclin and cyclin-dependent kinase genes by the adenovirus E1A gene product (E1A) or serum factors was studied with a rat 3Y1 derivative cell line, g12-21, in which the E1A12S cDNA can be expressed in response to dexamethasone (dex). The induction of DNA synthesis in quiescent g12-21 cel...

journal_title：Experimental cell research

authors： Ishii T,Shimizu M,Kanayama Y,Nakada S,Nojima H,Oda K

更新日期：1993-10-01 00:00:00

abstract：:X-ray-induced damage leads to cell-cycle "checkpoint" arrest by p53-dependent induction of the cyclin-dependent kinase inhibitor p21 (Waf1/Cip1/Sdi1). Human tumor cells that lack this response fail to arrest after exposure to DNA-damaging agents, undergo multiple rounds of endoreduplicative DNA synthesis, and eventual...

journal_title：Experimental cell research

authors： Denko NC,Green SL,Edwards D,Giaccia AJ

更新日期：2000-07-10 00:00:00

abstract：:The voltage-gated potassium channel, Kv1.3, plays an important role in regulating membrane excitability in diverse cell types ranging from T-lymphocytes to neurons. In the present study, we test the hypothesis that the C-terminal PDZ binding domain modulates the function and localization of Kv1.3. We created a mutant ...

journal_title：Experimental cell research

authors： Doczi MA,Damon DH,Morielli AD

更新日期：2011-10-01 00:00:00

abstract：:Phosphorylation of types III and IV intermediate filaments (IFs) is known to regulate their organization and function. Phosphorylation of the amino-terminal head domain sites on types III and IV IF proteins plays a key role in the assembly/disassembly of IF subunits into 10 nm filaments, and influences the phosphoryla...

journal_title：Experimental cell research

authors： Sihag RK,Inagaki M,Yamaguchi T,Shea TB,Pant HC

更新日期：2007-06-10 00:00:00

abstract：:We have studied the mechanisms of angiotensin II (A-II) transport through a cultured arterial endothelial cell monolayer. The transport of 125I-labeled A-II was inhibited by excess unlabeled A-II (50 microM) and [Sar1, Ile8]-A-II (50 microM), but was not inhibited by bradykinin (50 microM). The transport process was s...

journal_title：Experimental cell research

authors： Mineo C,Yagyu Y,Imanaka T,Takano T

更新日期：1990-09-01 00:00:00

abstract：:Tob, a member of the Tob and BTG antiproliferative protein family, plays an important role in many cellular processes including cell proliferation. In this study, we have addressed molecular mechanisms regulating subcellular localization of Tob. Treatment with leptomycin B, an inhibitor of nuclear export signal (NES) ...

journal_title：Experimental cell research

authors： Maekawa M,Yamamoto T,Nishida E

更新日期：2004-04-15 00:00:00

abstract：:A method for isolation of C-cells from rat fetuses was developed, and the morphological plasticity of the cells in primary culture systems was tested. Thyroid-parathyroid-ultimobranchial body (UB) complexes from 16-day rat fetuses were treated with 0.1% collagenase and 1000 PU/ml Dispase at 37 degrees C for 1 h. After...

journal_title：Experimental cell research

authors： Nishiyama I,Fujii T

更新日期：1992-02-01 00:00:00

abstract：:T cell polarization and redistribution of cellular components are critical to processes such as activation, migration, and potentially HIV infection. Here, we investigate the effects of CD4 engagement on the redistribution and localization of chemokine receptors, CXCR4 and CCR5, adhesion molecules, and lipid raft comp...

journal_title：Experimental cell research

authors： Nguyen DH,Giri B,Collins G,Taub DD

更新日期：2005-04-01 00:00:00

abstract：:Diabetic nephropathy (DN) is a major microvascular complication of diabetes that can lead to end-stage renal disease. Podocytes constitute the last barrier of glomerular filtration, whose damage are the direct cause of proteinuria. Dopamine receptors are involved in the regulation of diabetes-induced glomerular hyperf...

journal_title：Experimental cell research

authors： Shao X,Zhang X,Hu J,Gao T,Chen J,Xu C,Wei C

更新日期：2020-03-15 00:00:00

abstract：:We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-...

journal_title：Experimental cell research

authors： Kim H,You S,Kim IJ,Farris J,Foster LK,Foster DN

更新日期：2001-05-01 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is fundamental for normal cell growth and organ development, but has also been implicated in various pathologies, notably tumors of epithelial origin. We have previously shown that the initial 13 amino acids (P13) within the intracellular juxtamembrane region (R645-R657) are...

journal_title：Experimental cell research

authors： Aifa S,Aydin J,Nordvall G,Lundström I,Svensson SP,Hermanson O

更新日期：2005-01-01 00:00:00

abstract：:dUTP nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate and is the central regulator of cellular dUTP pools. Nuclear (DUT-N) and mitochondrial (DUT-M) isoforms of the protein have been identified in humans and arise from the same gene by the alternative use of 5' exons. Recently, ...

journal_title：Experimental cell research

authors： Tinkelenberg BA,Fazzone W,Lynch FJ,Ladner RD

更新日期：2003-07-01 00:00:00