当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Protein phosphatase 2A is involved in the regulation of protein kinase A signaling pathway during in vitro chondrogenesis.

Protein phosphatase 2A is involved in the regulation of protein kinase A signaling pathway during in vitro chondrogenesis.

Abstract：

:We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecule. When 20 nM OA was applied to the HDCs on Days 2 and 3 of culturing, it significantly inhibited protein phosphatase 2A (PP2A), enhanced cartilage formation, and elevated the activity of cAMP-dependent protein kinase (PKA). Application of 20 microM H89 significantly decreased the activity of PKA and blocked the chondrogenesis in HDCs. Furthermore, OA enhanced cartilage formation and elevated the suppressed activity of PKA even in the H89-pretreated HDCs. cGMP-dependent protein kinase was not detected in HDCs, while protein kinase Cmu (PKCmu), which is also inhibited by nanomolar concentrations of H89, was present throughout the culturing period. Neither OA nor H89 influenced the expression of the catalytic subunit of PKA or the cAMP response element binding protein, CREB. However, a significantly elevated amount of Ser-133-phosphorylated-CREB (P-CREB) was detected following addition of OA, while H89 treatment resulted in a decrease of the amount of P-CREB. Our results demonstrate that PP2A plays a role in the regulation of the PKA signaling pathway and that the phosphorylation level of CREB is influenced by the activity of both enzymes during in vitro chondrogenesis.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Zákány R,Szucs K,Bakó E,Felszeghy S,Czifra G,Bíró T,Módis L,Gergely P

doi

10.1006/excr.2002.5487

keywords:

subject

Has Abstract

pub_date

2002-04-15 00:00:00

pages

1-8

issue

1

eissn

0014-4827

issn

1090-2422

pii

S001448270295487X

journal_volume

275

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • An antigen expressed in proliferating cells at late G1-S phase.

    abstract：:A monoclonal antibody Pr-28 was prepared, which recognized an antigen present only in proliferating cells. Immunofluorescence analysis of Pr-28 antigen showed that the antigen was localized mainly in perinuclear cytoplasm. Although Pr-28 antibody was produced against a chicken cell antigen, it reacts not only with chi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90312-0

    authors： Matsuhashi S,Watanabe T,Hori K

    更新日期：1987-06-01 00:00:00

  • Long non-coding RNA HNF1A-AS1 upregulates OTX1 to enhance angiogenesis in colon cancer via the binding of transcription factor PBX3.

    abstract：:Colon cancer shows characteristics of metastasis, which is associated with angiogenesis. Increasing evidence highlights long non-coding RNAs (lncRNAs) as important participants in angiogenesis of cancers, including colon cancer. Hence, this study investigated the role of HNF1A-AS1 in angiogenesis of colon cancer. RT-q...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112025

    authors： Wu J,Meng X,Jia Y,Chai J,Wang J,Xue X,Dang T

    更新日期：2020-08-15 00:00:00

  • In vitro culture of mouse primordial germ cells.

    abstract：:Germ cells were isolated from mouse fetal gonads 11 1/2-16 1/2 days post coitum (dpc), and exposed to various methods of in vitro culture. From 13 1/2 dpc onwards, both male and female germ cells survived well at 37 degrees C for several days. During the culture period the proportion of female germ cells in meiosis in...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90421-4

    authors： De Felici M,McLaren A

    更新日期：1983-04-01 00:00:00

  • Glorin-regulated protein synthesis in Polyspondylium violaceum.

    abstract：:Protein synthesis by Polyspondylium violaceum in response to the chemoattractant glorin was analyzed by two-dimensional gel electrophoresis to determine if glorin can affect gene expression. When cells developing in a shaking suspension culture were given glorin, five proteins exhibited significantly increased and thr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90323-3

    authors： Kopachik W

    更新日期：1990-02-01 00:00:00

  • p21ras. Heterogeneous localization in transformed cells.

    abstract：:The cellular targets for the Kirsten murine sarcoma virus (KiMSV)-transforming protein, p21ras, are unknown. Other studies have indicated that the mature form of p21 is distributed diffusely on the cytoplasmic face of the plasma membrane. However, after fixation without buffer washes, indirect immunofluorescent staini...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(85)80017-3

    authors： Myrdal SE,Auersperg N

    更新日期：1985-08-01 00:00:00

  • Cell-cycle-dependent regulation of cell motility and determination of the role of Rac1.

    abstract：:To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three ke...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.01.011

    authors： Walmod PS,Hartmann-Petersen R,Prag S,Lepekhin EL,Röpke C,Berezin V,Bock E

    更新日期：2004-05-01 00:00:00

  • Identification of integrins involved in cell adhesion to native and denatured type I collagens and the phenotypic transition of rabbit arterial smooth muscle cells.

    abstract：:Rabbit smooth muscle cells (SMC) in primary culture attached to and started proliferating on native and heat-denatured type I collagens, although the amount of cell attachment to denatured collagen was significantly lower. The cells adhered poorly and were unable to grow on commercial gelatin. In contrast, synthetic S...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1225

    authors： Yamamoto M,Yamato M,Aoyagi M,Yamamoto K

    更新日期：1995-07-01 00:00:00

  • Human autoantibody to topoisomerase II.

    abstract：:The rheumatic diseases are characterized by the production of autoantibodies that are usually directed against components of the cell nucleus. In this communication, we describe autoantibodies that recognize DNA topoisomerase II (anti-topoII) present in the serum of a patient with systemic lupus erythematosus. Several...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90067-0

    authors： Hoffmann A,Heck MM,Bordwell BJ,Rothfield NF,Earnshaw WC

    更新日期：1989-02-01 00:00:00

  • Behavior of the cell cycle-associated proteins in an unusual G0-arrestable cancer cell line.

    abstract：:An adenocarcinoma cell line which has the ability to arrest in G0 phase under exhausted culture conditions was established. Using the cell line, we investigated the expression of cell cycle-associated proteins including cdc2, cdk2, cyclin A, cyclin Dl, and Rb during entry into or withdrawal from the cell cycle. MAP ki...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0159

    authors： Ohta T,Fukuda M,Wanebo HJ,Jogo K,Yamaguchi S

    更新日期：1996-05-25 00:00:00

  • Probing effects of pH change on dynamic response of Claudin-2 mediated adhesion using single molecule force spectroscopy.

    abstract：:Claudins belong to a large family of transmembrane proteins that localize at tight junctions (TJs) where they play a central role in regulating paracellular transport of solutes and nutrients across epithelial monolayers. Their ability to regulate the paracellular pathway is highly influenced by changes in extracellul...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.05.015

    authors： Lim TS,Vedula SR,Hui S,Kausalya PJ,Hunziker W,Lim CT

    更新日期：2008-08-15 00:00:00

  • The beta 4 integrin subunit is expressed in mouse fibroblasts and modulated by transforming growth factor-beta 1.

    abstract：:Integrin beta 4 subunit is present in association with alpha 6 chain on both normal and transformed epithelial cells. Recently alpha 6 beta 4 heterodimer was found on the endothelium of medium-sized blood vessels and on immature thymocytes. In this report we show, by Northern blotting, indirect immunofluorescence, imm...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0271

    authors： Scardigli R,Soddu S,Falcioni R,Crescenzi M,Cimino L,Sacchi A

    更新日期：1996-09-15 00:00:00

  • Ontogeny of basic fibroblast growth factor binding sites in mouse ocular tissues.

    abstract：:Basic fibroblast growth factor (bFGF) binding to ocular tissues has been studied by autoradiographical and biochemical approaches directly performed on sections during mouse embryonic and postnatal development. Frozen sections of embryos (9 to 18 days), newborns, and adults (1 day to 6 months) were incubated with iodi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90280-n

    authors： Fayein NA,Courtois Y,Jeanny JC

    更新日期：1990-05-01 00:00:00

  • CD25-expressing Th17 cells mediate CD8+ T cell suppression in CTLA-4 dependent mechanisms in pancreatic ductal adenocarcinoma.

    abstract：:The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.09.030

    authors： Lang C,Wang J,Chen L

    更新日期：2017-11-15 00:00:00

  • Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans.

    abstract：:The mammalian Hippo pathway comprises mammalian Ste20-like kinases (MST1/2) and large tumor suppressor kinases (LATS1/2). LATS1/2, which are activated by MST1/2, phosphorylate a transcriptional co-activator, yes-associated protein (YAP), and induce the recruitment of YAP by 14-3-3 to cytoplasm, so that the TEAD-depend...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.01.020

    authors： Iwasa H,Maimaiti S,Kuroyanagi H,Kawano S,Inami K,Timalsina S,Ikeda M,Nakagawa K,Hata Y

    更新日期：2013-04-15 00:00:00

  • CP beta3, a novel isoform of an actin-binding protein, is a component of the cytoskeletal calyx of the mammalian sperm head.

    abstract：:In the mammalian sperm head, the nucleus is tightly associated with the calyx, a cell type-specific cytoskeletal structure. Previously, we have identified and characterized some basic proteins such as calicin and cylicins I and II as major calyx components of bovine and human spermatids and spermatozoa. Surprisingly w...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3564

    authors： von Bülow M,Rackwitz HR,Zimbelmann R,Franke WW

    更新日期：1997-05-25 00:00:00

  • Induction of M-phase entry of prophase-blocked mouse oocytes through microinjection of okadaic acid, a specific phosphatase inhibitor.

    abstract：:We report that a specific inhibitor of types 1 and 2A phosphatases, okadaic acid (OA), induces germinal vesicle break down (GVBD) and chromosome condensation when microinjected into denuded mouse oocytes maintained in prophase block by analogs of cAMP, inhibitors of phosphodiesterase, or a tumor-promoting phorbol este...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90159-r

    authors： Gavin AC,Tsukitani Y,Schorderet-Slatkine S

    更新日期：1991-01-01 00:00:00

  • R5, a neutralizing antibody to Robo1, suppresses breast cancer growth and metastasis by inhibiting angiogenesis via down-regulating filamin A.

    abstract：:Breast cancer (BC) is one of the most common cancers among women in both developed and developing countries with a rising incidence. Using the MMTV-PyMT transgenic mouse model and xenografted breast cancer model, we found that R5, a neutralizing antibody to Robo1, significantly inhibited BC growth and metastasis. Angi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111756

    authors： Li Q,Cao J,He Y,Liu X,Mao G,Wei B,Liao S,Zhang Q,Li J,Zheng L,Wang L,Qi C

    更新日期：2020-02-01 00:00:00

  • Characteristics of 25-hydroxycholesterol-induced apoptosis in the human leukemic cell line CEM.

    abstract：:Cholesterol and related compounds can give rise to oxygenated sterol molecules (oxysterols) which are potent regulators of lymphoid cell growth. Oxysterols added exogenously cause cell death of several lines of cultured cells, and on the basis of limited criteria, it has been suggested that this death is apoptosis. In...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3630

    authors： Ayala-Torres S,Moller PC,Johnson BH,Thompson EB

    更新日期：1997-08-25 00:00:00

  • Laminin isoforms: biological roles and effects on the intracellular distribution of nuclear proteins in intestinal epithelial cells.

    abstract：:Laminins are structurally and functionally major components of the extracellular matrix. Four isoforms of laminins (laminin-1, -2, -5 and -10) are expressed in a specific pattern along the crypt-villus axis of the intestine. Previous works indicated that expression of these isoforms is developmentally regulated and th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.10.025

    authors： Turck N,Gross I,Gendry P,Stutzmann J,Freund JN,Kedinger M,Simon-Assmann P,Launay JF

    更新日期：2005-02-15 00:00:00

  • Effector mechanisms of fenretinide-induced apoptosis in neuroblastoma.

    abstract：:Fenretinide is an effective inducer of apoptosis in many malignancies but its precise mechanism(s) of action in the induction of apoptosis in neuroblastoma is unclear. To characterize fenretinide-induced apoptosis, neuroblastoma cell lines were treated with fenretinide and flow cytometry was used to measure apoptosis,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4988

    authors： Lovat PE,Ranalli M,Annichiarrico-Petruzzelli M,Bernassola F,Piacentini M,Malcolm AJ,Pearson AD,Melino G,Redfern CP

    更新日期：2000-10-10 00:00:00

  • Stromal cell-derived factor-1alpha (SDF-1alpha/CXCL12) stimulates ovarian cancer cell growth through the EGF receptor transactivation.

    abstract：:Ovarian cancer (OC) is the leading cause of death in gynecologic diseases in which there is evidence for a complex chemokine network. Chemokines are a family of proteins that play an important role in tumor progression influencing cell proliferation, angiogenic/angiostatic processes, cell migration and metastasis, and...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.04.024

    authors： Porcile C,Bajetto A,Barbieri F,Barbero S,Bonavia R,Biglieri M,Pirani P,Florio T,Schettini G

    更新日期：2005-08-15 00:00:00

  • Nonmuscle and smooth muscle myosin isoforms in bovine endothelial cells.

    abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90136-x

    authors： Borrione AC,Zanellato AM,Giuriato L,Scannapieco G,Pauletto P,Sartore S

    更新日期：1990-09-01 00:00:00

  • MPF amplification in Xenopus oocyte extracts depends on a two-step activation of cdc25 phosphatase.

    abstract：:The activation of Cdc2 kinase induces the entry into M-phase of all eukaryotic cells. We have developed a cell-free system prepared from prophase-arrested Xenopus oocytes to analyze the mechanism initiating the all-or-none activation of Cdc2 kinase. Inhibition of phosphatase 2A, the major okadaic acid-sensitive Ser/Th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4220

    authors： Karaïskou A,Cayla X,Haccard O,Jessus C,Ozon R

    更新日期：1998-11-01 00:00:00

  • A population of serum deprivation-induced bone marrow stem cells (SD-BMSC) expresses marker typical for embryonic and neural stem cells.

    abstract：:The bone marrow represents an easy accessible source of adult stem cells suitable for various cell based therapies. Several studies in recent years suggested the existence of pluripotent stem cells within bone marrow stem cells (BMSC) expressing marker proteins of both embryonic and tissue committed stem cells. These ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.10.007

    authors： Sauerzweig S,Munsch T,Lessmann V,Reymann KG,Braun H

    更新日期：2009-01-01 00:00:00

  • Proteins that associate with lamins: many faces, many functions.

    abstract：:Lamin-associated polypeptides (LAPs) comprise inner nuclear membrane proteins tightly associated with the peripheral lamin scaffold as well as proteins forming stable complexes with lamins in the nucleoplasm. The involvement of LAPs in a wide range of human diseases may be linked to an equally bewildering range of the...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2007.03.012

    authors： Schirmer EC,Foisner R

    更新日期：2007-06-10 00:00:00

  • Integrin alpha6 cleavage: a novel modification to modulate cell migration.

    abstract：:Integrins play a major role in cell adhesion and migration. Previous work reported that a cleaved form of integrin alpha6 (alpha6p) was detected in invasive human prostate cancer tissue, absent in normal prostate tissue and was produced by urokinase-type Plasminogen Activator (uPA) in a plasmin-independent manner. Usi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.01.006

    authors： Pawar SC,Demetriou MC,Nagle RB,Bowden GT,Cress AE

    更新日期：2007-04-01 00:00:00

  • The phosphorylation status and cytoskeletal remodeling of striatal astrocytes treated with quinolinic acid.

    abstract：:Quinolinic acid (QUIN) is a glutamate agonist which markedly enhances the vulnerability of neural cells to excitotoxicity. QUIN is produced from the amino acid tryptophan through the kynurenine pathway (KP). Dysregulation of this pathway is associated with neurodegenerative conditions. In this study we treated striata...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.02.024

    authors： Pierozan P,Ferreira F,Ortiz de Lima B,Gonçalves Fernandes C,Totarelli Monteforte P,de Castro Medaglia N,Bincoletto C,Soubhi Smaili S,Pessoa-Pureur R

    更新日期：2014-04-01 00:00:00

  • Resistance of Chinese hamster ovary cell chromatin to endonuclease digestion. I. Reversal by cAMP.

    abstract：:A portion of the DNA within intact nuclei of a spontaneously transformed Chinese hamster ovary cell line (CHO-Kl) is relatively resistant to digestion by pancreatic deoxyribonuclease, as compared to nuclei from primary cultures of Chinese hamster ovary fibroblasts. Treatment of CHO-Kl cells with derivatives of 3',5' c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(83)80007-x

    authors： Schonberg S,Patterson D,Puck TT

    更新日期：1983-04-15 00:00:00

  • Establishment and characterization of a porcine B cell lymphoma cell line.

    abstract：:The lack of available, well characterized, established, domestic porcine cell lines hinders the advancement of porcine cellular immunology. A case of multicentric lymphoma was diagnosed in a market weight pig at the time of slaughter. Affected lymph nodes and spleen were collected and used for single cell isolation an...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.111986

    authors： Rahe MC,Dvorak CMT,Wiseman B,Martin D,Murtaugh MP

    更新日期：2020-05-15 00:00:00

  • Establishment of medakafish as a model for stem cell-based gene therapy: efficient gene delivery and potential chromosomal integration by baculoviral vectors.

    abstract：:Viral vectors hold promise and challenges in gene therapy. Specifically, we have previously shown that baculoviral (BV) vectors have a high efficiency of gene delivery in human embryonic stem (ES) cells. Here we report the development of a complementary system to further our evaluation by utilizing the laboratory fish...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.04.015

    authors： Yan Y,Du J,Chen T,Yi M,Li M,Wang S,Li CM,Hong Y

    更新日期：2009-08-01 00:00:00