Induction of apoptosis in human replicative senescent fibroblasts.


:Cellular senescence is an irreversible growth phase characteristic of normal cells. We have found that human senescent fibroblasts can be induced to undergo programmed cell death (apoptosis) by ceramide, TNF-alpha, or okadaic acid. The most profound effects were induced by TNF-alpha and okadaic acid treatment. In the present study, we also evaluated the contribution of lysosomal activation as a possible mechanism underlying the induction of apoptosis. Four lysosomal enzyme activities were measured: beta-galactosidase, alpha-galactosidase A, beta-glucoronidase, and acid phosphatase. Using an in situ assay, we have found that the activity of beta-galactosidase, which is also a biochemical marker of senescence, is induced in young proliferating fibroblasts following exposure to all three apoptotic inducing agents. The other enzymes were not significantly induced in young fibroblasts following exposure to agents that induce apoptosis. During replicative senescence, three of the four lysosomal enzymes tested (beta-galactosidase, alpha-galactosidase A, and beta-glucoronidase) are constitutively expressed at high levels. TNF-alpha was the only agent that induced lysosomal activity in senescent fibroblasts, of which only alpha-galactosidase A activity was induced. Our studies show that senescent fibroblasts can be induced to undergo apoptosis in a signal-dependent manner. However, the lysosomal enzymes examined do not appear to be correlated with apoptotic induction.


Exp Cell Res


DeJesus V,Rios I,Davis C,Chen Y,Calhoun D,Zakeri Z,Hubbard K





Has Abstract


2002-03-10 00:00:00














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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Theus MH,Wei L,Francis K,Yu SP

    更新日期:2006-10-01 00:00:00

  • Bone morphogenetic protein-induced cell differentiation involves Atg7 and Wnt16 sequentially in human stem cell-derived osteoblastic cells.

    abstract::We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...

    journal_title:Experimental cell research

    pub_type: 杂志文章,收录出版


    authors: Ozeki N,Mogi M,Hase N,Hiyama T,Yamaguchi H,Kawai R,Matsumoto T,Nakata K

    更新日期:2016-09-10 00:00:00

  • Conjugation in the ciliate Euplotes octocarinatus: comparison of ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells.

    abstract::Pair formation in the hypotrichous ciliate Euplotes octocarinatus is a poorly understood phenomenon. In order to obtain information about the molecules involved in this process, we compared ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells. Detection of g...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Plümper E,Freiburg M,Heckmann K

    更新日期:1995-04-01 00:00:00

  • Tissue-specific posttranslational modification of the small heat shock protein HSP27 in Drosophila.

    abstract::Drosophila sHSPs (small heat shock proteins) are expressed in the absence of stress in specific regions of the central nervous system and in gonads of young adults flies. In these two organs, the sHSPs show a cell-specific and developmental stage-specific pattern of expression suggesting distinct regulation and functi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Marin R,Landry J,Tanguay RM

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  • Secretory phospholipase A(2) inhibits epidermal growth factor-induced receptor activation.

    abstract::Secretory phospholipase A(2) (sPLA(2)) plays important roles in mediating various cellular processes, including cell proliferation, differentiation, apoptosis, and inflammatory response. In this study, we demonstrated that a basic sPLA(2) inhibits epidermal growth factor (EGF)-induced EGF receptor activation, as deter...

    journal_title:Experimental cell research

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    authors: Zhao S,Du XY,Chen JS,Zhou YC,Song JG

    更新日期:2002-10-01 00:00:00

  • Characteristics of 25-hydroxycholesterol-induced apoptosis in the human leukemic cell line CEM.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ayala-Torres S,Moller PC,Johnson BH,Thompson EB

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    journal_title:Experimental cell research

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    authors: Vanderpuye OA,Carraway CA,Carraway KL

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  • Mechanical regulation of myofibroblast phenoconversion and collagen contraction.

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    journal_title:Experimental cell research

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    authors: Hinz B,McCulloch CA,Coelho NM

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    authors: Eriksson T,Frisk T,Gray SG,von Schweinitz D,Pietsch T,Larsson C,Sandstedt B,Ekström TJ

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  • Alteration of interendothelial adherens junctions following tumor cell-endothelial cell interaction in vitro.

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    journal_title:Experimental cell research

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    authors: Lewalle JM,Bajou K,Desreux J,Mareel M,Dejana E,Noël A,Foidart JM

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    journal_title:Experimental cell research

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    authors: Minato N

    更新日期:2013-09-10 00:00:00

  • Recruitment of phosphorylated small heat shock protein Hsp27 to nuclear speckles without stress.

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    authors: Bryantsev AL,Chechenova MB,Shelden EA

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    authors: Hong SK,Jeong JH,Chan AM,Park JI

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    journal_title:Experimental cell research

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    authors: Jagiello G,Sung WK,Van't Hof J

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    authors: Rolland Y,Demeule M,Michaud-Levesque J,Béliveau R

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  • Immunofluorescent localization of histone H10 in the nuclei of proliferating and differentiating Friend cells.

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  • Identification of vinculin as a pericentriolar component in mammalian cells.

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    authors: Chevrier V,Paintrand M,Koteliansky V,Block MR,Job D

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  • siRNA - Mediated LRP/LR knock-down reduces cellular viability of malignant melanoma cells through the activation of apoptotic caspases.

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    authors: Rebelo TM,Vania L,Ferreira E,Weiss SFT

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    authors: Dupont S

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    authors: Hergott GJ,Kalnins VI

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    journal_title:Experimental cell research

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    authors: Razin E,Baranes D,Marx G

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  • Isolation and immunohistochemical characterization of two developmentally regulated highly insoluble antigens from the membrane of sinusoidal rat liver cells.

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    authors: Zanetta JP,Bingen A,Dontenwill M,Vincendon G

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li X,Darzynkiewicz Z

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    pub_type: 杂志文章


    authors: Liao XH,Li JY,Dong XM,Wang X,Xiang Y,Li H,Yu CX,Li JP,Yuan BY,Zhou J,Zhang TC

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  • The murine PKR tumor suppressor gene is rearranged in a lymphocytic leukemia.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Abraham N,Jaramillo ML,Duncan PI,Méthot N,Icely PL,Stojdl DF,Barber GN,Bell JC

    更新日期:1998-11-01 00:00:00

  • Cultured endothelial cells produce multiple growth factors for connective tissue cells.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: DiCorleto PE

    更新日期:1984-07-01 00:00:00

  • Cell cycle-dependent DNA damage signaling induced by ICRF-193 involves ATM, ATR, CHK2, and BRCA1.

    abstract::Topoisomerase II is essential for cell proliferation and survival and has been a target of various anticancer drugs. ICRF-193 has long been used as a catalytic inhibitor to study the function of topoisomerase II. Here, we show that ICRF-193 treatment induces DNA damage signaling. Treatment with ICRF-193 induced G2 arr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Park I,Avraham HK

    更新日期:2006-07-01 00:00:00