Involvement of LPA signaling via LPA receptor-2 in the promotion of malignant properties in osteosarcoma cells.

abstract：:In our recent studies, we have demonstrated that monolayer cultures of bovine aortic endothelial (BAE) cells that do not express type I collagen also fail to express and synthesize decorin, a small chondroitin/dermatan sulfate proteoglycan that interacts with type I collagen and regulates collagen fibrillogenesis in v...

journal_title：Experimental cell research

authors： Järveläinen HT,Iruela-Arispe ML,Kinsella MG,Sandell LJ,Sage EH,Wight TN

更新日期：1992-12-01 00:00:00

abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

journal_title：Experimental cell research

authors： Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

更新日期：2008-08-01 00:00:00

abstract：:To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three ke...

journal_title：Experimental cell research

authors： Walmod PS,Hartmann-Petersen R,Prag S,Lepekhin EL,Röpke C,Berezin V,Bock E

更新日期：2004-05-01 00:00:00

abstract：:Coiled bodies (CBs) are structural constituents observed in nuclei of most eukaryotic cells. They usually occur in the nucleoplasm as well as in contact with the nucleolar surface. In this work we studied the hepatocyte nuclei of hibernating dormice in order to investigate possible modifications of CBs along the seaso...

journal_title：Experimental cell research

authors： Malatesta M,Zancanaro C,Martin TE,Chan EK,Amalric F,Lührmann R,Vogel P,Fakan S

更新日期：1994-04-01 00:00:00

abstract：:Microfilament cores, obtained by extracting 13762 mammary ascites tumor cell microvilli with Triton X-100, contain a major glycoprotein migrating at an apparent molecular weight of 80 kDa by dodecyl sulfate-polyacrylamide gel electrophoresis. The 80-kDa component is a disulfide-linked multimer, as demonstrated by velo...

journal_title：Experimental cell research

authors： Ye XH,Metcalf TN 3rd,Andrews DM,Carraway CA,Carraway KL

更新日期：1989-05-01 00:00:00

abstract：:Caveolin-1 (Cav-1) is a principal structural protein of caveolae. Cav-1 has been implicated in cancer progression, but its precise functional roles in pituitary adenoma cells remain largely unclear. In this study, we evidenced that the level of cav-1 was elevated in the invasive pituitary adenoma. Cav-1 knockdown rest...

journal_title：Experimental cell research

authors： Yang W,Xu T,Qiu P,Xu G

更新日期：2018-06-01 00:00:00

abstract：:It is known that freshly dissociated thyroid cell clusters form follicles in suspension culture. Thyroid epithelial cell lines, grown for many generations in vitro, fail to show colloid-containing lumina when cultured as monolayers. Several thyroid cell lines, some transformed, have been tested with respect to their a...

journal_title：Experimental cell research

authors： Nitsch L,Tacchetti C,Tramontano D,Ambesi-Impiombato FS

更新日期：1984-05-01 00:00:00

abstract：:We have investigated the gene targeting frequency at the hprt locus in a range of embryonic stem cell lines selected for variations in cell cycle parameters. Our results show that targeting frequency varies with cell line by as much as 12-fold between nonisogenic lines and 3-fold between isogenic lines and that a noni...

journal_title：Experimental cell research

authors： Udy GB,Parkes BD,Wells DN

更新日期：1997-03-15 00:00:00

abstract：:Huntingtin interacting protein HYPK (Huntingtin Yeast Partner K) is an intrinsically unstructured protein having chaperone-like activity and can suppress mutant huntingtin aggregates and toxicity in cell model of Huntington's Disease (HD). Heat shock response is an adaptive mechanism of cells characterized by upregula...

journal_title：Experimental cell research

authors： Das S,Bhattacharyya NP

更新日期：2016-05-01 00:00:00

abstract：:Spindle assembly is essential for the equal distribution of genetic material to the daughter cells during mitosis. The process of spindle assembly is complicated and involves multiple levels of molecular regulation. It is generally accepted that mitotic spindles are emanated from the centrosomes and are assembled in t...

journal_title：Experimental cell research

authors： Yang CP,Fan SS

更新日期：2008-10-15 00:00:00

abstract：:Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP-110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recr...

journal_title：Experimental cell research

authors： Crimaldi L,Courtneidge SA,Gimona M

更新日期：2009-09-10 00:00:00

abstract：:Herp (homocysteine-induced endoplasmic reticulum protein) is an ER-resident membrane protein, which has a ubiquitin-like domain at its N-terminus. Expression of Herp protein is up-regulated in response to ER stress, including homocysteine. Herp stabilizes neuronal Ca(2+) homeostasis and is involved in improving the ba...

journal_title：Experimental cell research

authors： Lenz B,Bleich S,Beutler S,Schlierf B,Schwager K,Reulbach U,Kornhuber J,Bönsch D

更新日期：2006-12-10 00:00:00

abstract：:Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...

journal_title：Experimental cell research

authors： Gazziola C,Moras M,Ferraro P,Gallinaro L,Verin R,Rampazzo C,Reichard P,Bianchi V

更新日期：1999-12-15 00:00:00

abstract：:An earlier report indicated that a 26-amino-acid peptide (SA), comprised of the nuclear localization signal (NLS) of fibroblast growth factor-1 (FGF-1) and a membrane-permeable peptide, was able to stimulate DNA synthesis after it was taken up by NIH3T3 fibroblasts. Here, we report that SA, but not a mutant with the N...

journal_title：Experimental cell research

authors： Komi A,Suzuki M,Imamura T

更新日期：1998-09-15 00:00:00

abstract：:Transforming growth factor beta (TGFbeta) can modulate the activity of various MAP kinases. However, how this pathway may mediate TGFbeta-induced malignant phenotypes remains elusive. We investigated the role of autocrine TGFbeta signaling through MAP kinases in the regulation of cell survival in breast carcinoma MCF-...

journal_title：Experimental cell research

authors： Lei X,Yang J,Nichols RW,Sun LZ

更新日期：2007-05-01 00:00:00

abstract：:Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in t...

journal_title：Experimental cell research

authors： Iarovaia O,Razin SV,Linares-Cruz G,Sjakste N,Scherrer K

更新日期：2001-11-01 00:00:00

abstract：:Ezrin-Radixin-Moesin (ERM) binding phosphoprotein 50 (EBP50, a.k.a. NHERF-1) is a scaffold protein essential for the localization and coordinated activity of apical transporters, enzymes and receptors in epithelial cells. EBP50 acts via multiple protein binding interactions, including oligomerization through interacti...

journal_title：Experimental cell research

authors： Fouassier L,Nichols MT,Gidey E,McWilliams RR,Robin H,Finnigan C,Howell KE,Housset C,Doctor RB

更新日期：2005-05-15 00:00:00

abstract：:The mitochondrion depends upon the import of cytosolically synthesized preproteins for most of the proteins that comprise its structural elements and metabolic pathways. Here we have examined the influence of redox conditions on mitochondrial preprotein import and processing by mammalian mitochondria. Paraquat pretrea...

journal_title：Experimental cell research

authors： Wright G,Terada K,Yano M,Sergeev I,Mori M

更新日期：2001-02-01 00:00:00

abstract：:Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of v...

journal_title：Experimental cell research

authors： Yuan A,Chia CP

更新日期：2000-12-15 00:00:00

abstract：:Hypopharyngeal squamous cell carcinoma (HSCC) is a relatively rare malignancy and has the worst prognosis among head and neck cancer. Metastasis is the major cause of poor prognosis in HSCC patients. In this study, we found that 3-phosphoinositide-dependent protein kinase 1 (PDK1 or PDPK1) was overexpressed in HSCC. T...

journal_title：Experimental cell research

authors： Jing P,Zhou S,Xu P,Cui P,Liu X,Liu X,Liu X,Wang H,Xu W

更新日期：2020-01-15 00:00:00

abstract：:Translation is mediated partly by regulation of free eukaryotic initiation factor 4E (eIF4E) levels through PI3K-Akt-mTOR signaling. Cancer cells treated with the plant-derived perillyl alcohol (POH) or the mechanistic target of rapamycin (mTOR) inhibitor rapamycin dephosphorylate eIF4E-binding protein (4E-BP1) and at...

journal_title：Experimental cell research

authors： Sundin T,Peffley D,Hentosh P

更新日期：2013-08-01 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00

abstract：:We previously showed that the phenotypes of adipocyte progenitors and macrophages were close. Using functional analyses and microarray technology, we first tested whether this intriguing relationship was specific to adipocyte progenitors or could be shared with other progenitors. Measurements of phagocytic activity an...

journal_title：Experimental cell research

authors： Charrière GM,Cousin B,Arnaud E,Saillan-Barreau C,André M,Massoudi A,Dani C,Pénicaud L,Casteilla L

更新日期：2006-10-15 00:00:00

abstract：:By a sequential mutation and selection utilizing N-methyl-N'-nitro-N-nitrosoguanidine as a mutagen, we succeeded in separating a poly(ADP ribose) polymerase-defective mutant clone (Cl-3527) from a mouse L1210 cell clone (Cl-3). The enzyme activity per cell in Cl-3527 cells was only 8% of that in wild type L1210 (CCL 2...

journal_title：Experimental cell research

authors： Yoshihara K,Itaya A,Hironaka T,Sakuramoto S,Tanaka Y,Tsuyuki M,Inada Y,Kamiya T,Ohnishi K,Honma M

更新日期：1992-05-01 00:00:00

abstract：:Spinal muscular atrophy (SMA), a recessive, neuromuscular disease, is caused by a mutation or deletion in the SMN1 gene. The SMN2 gene is present in the same region of chromosome 5 and is similar in DNA sequence to SMN1 except for a T at position +6 of exon 7 that is likely the predominant functional difference betwee...

journal_title：Experimental cell research

authors： DiMatteo D,Callahan S,Kmiec EB

更新日期：2008-02-15 00:00:00

abstract：:We examined the transcriptional activity profile of the gene for atrial natriuretic factor (ANF) in mouse embryonal carcinoma P19 cells which had been induced for in vitro cardiac myogenesis. Differentiation was assessed visually, by the degree of spontaneous beating activity, and by the appearance of striated muscle ...

journal_title：Experimental cell research

authors： Boer PH

更新日期：1993-08-01 00:00:00

abstract：:Although periodontal diseases are initiated by bacteria that colonize the tooth surface and gingival sulcus, the host response is believed to play an essential role in the breakdown of connective tissue and bone. Mitochondrial reactive oxygen species (mtROS) have been proposed to regulate the activation of the inflamm...

journal_title：Experimental cell research

authors： Li X,Wang X,Zheng M,Luan QX

更新日期：2016-09-10 00:00:00

abstract：:Among the cellular events that are associated with the process of endochondral ossification is an incremental increase in chondrocyte basal intracellular free Ca(2+) concentration ([Ca(2+)](i)) from 50 to 100 nM. To determine if this rise in [Ca(2+)](i) functionally participates in the maturational process of growth p...

journal_title：Experimental cell research

authors： Zuscik MJ,D'Souza M,Ionescu AM,Gunter KK,Gunter TE,O'Keefe RJ,Schwarz EM,Puzas JE,Rosier RN

更新日期：2002-06-10 00:00:00

abstract：:The rapidity of the optional 90-min differentiation of Naegleria gruberi from amoebae to flagellates suggests the possibility of a free-running cascade of events from initiating stimulus through gene expression to organelle assembly and cell morphogenesis. Instead our experiments reveal two points early in the differe...

journal_title：Experimental cell research

authors： Fulton C,Lai EY

更新日期：1998-08-01 00:00:00

abstract：:Rap1 signaling is important for both major processes of vessel formation: vasculogenesis, or de novo vessel formation, and angiogenesis, sprouting of new vessels from pre-existing ones. We provide an overview of genetic studies in mice and zebrafish and discuss some of the proposed underlying mechanisms derived from c...

journal_title：Experimental cell research

authors： Chrzanowska-Wodnicka M

更新日期：2013-09-10 00:00:00