Abstract：
:Microfilament cores, obtained by extracting 13762 mammary ascites tumor cell microvilli with Triton X-100, contain a major glycoprotein migrating at an apparent molecular weight of 80 kDa by dodecyl sulfate-polyacrylamide gel electrophoresis. The 80-kDa component is a disulfide-linked multimer, as demonstrated by velocity sedimentation and agarose-acrylamide gel electrophoresis analyses under nonreducing conditions. This 80-kDa species is not metabolically labeled, as is a minor 80-kDa glycoprotein found in the cores, membranes, and an isolated transmembrane complex with actin. Antibodies prepared against the 80-kDa glycoprotein react strongly with bovine IgM and more weakly with rat IgM. These antibodies were used to demonstrate that the 80-kDa component is present in microvilli, microvillar microfilament cores, and microvillar membranes only if the microvilli are prepared in the presence of calf serum. The 80-kDa component, purified by velocity sedimentation in dodecyl sulfate, reacts with anti-rat IgM by immunoblot analyses. Moreover, immunoprecipitation of detergent extracts of microvilli with anti-rat IgM specifically sediments the 80-kDa component. The 80-kDa glycoprotein fractionates with the actin-containing transmembrane complex prepared by gel filtration of Triton-solubilized microvillar membranes. These results indicate that the disulfide-linked, multi-meric 80-kDa component is bovine IgM, which binds strongly to a cell-surface component of the microvilli, and is indirectly associated with the microfilament cores. Thus, the IgM provides a marker by which the transmembrane complexes to the microfilaments can be identified.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ye XH,Metcalf TN 3rd,Andrews DM,Carraway CA,Carraway KLdoi
10.1016/0014-4827(89)90288-7subject
Has Abstractpub_date
1989-05-01 00:00:00pages
160-72issue
1eissn
0014-4827issn
1090-2422journal_volume
182pub_type
杂志文章abstract：:Up-regulation of intercellular adhesion molecule-1 (ICAM-1) and its soluble form are involved in the chronic inflammation. For the first time, we demonstrated that resveratrol (RE), a natural polyphenol with antioxidant and anti-inflammatory properties, reduces the increase of expression and release of ICAM-1, due to ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.06.024
更新日期：2019-09-15 00:00:00
abstract：:A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90604-9
更新日期：1984-08-01 00:00:00
abstract：:The mammalian Hippo pathway comprises mammalian Ste20-like kinases (MST1/2) and large tumor suppressor kinases (LATS1/2). LATS1/2, which are activated by MST1/2, phosphorylate a transcriptional co-activator, yes-associated protein (YAP), and induce the recruitment of YAP by 14-3-3 to cytoplasm, so that the TEAD-depend...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.01.020
更新日期：2013-04-15 00:00:00
abstract：:O-GlcNAc transferase (OGT)-mediated modification of protein Ser/Thr residues with O-GlcNAc influences protein activity, similar to the effects of phosphorylation. The anti-apoptotic Akt1 is both activated by phosphorylation and modified with O-GlcNAc. However, the nature and significance of the Akt1 O-GlcNAc modificat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.04.014
更新日期：2008-07-01 00:00:00
abstract：:We present evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence. Human fibroblasts immortalized with an inducible SV40 T-antigen become senescent following the de-induction of T-antigen. Plasmids expressing an alternative source of intact T-antigen restore prol...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90453-2
更新日期：1991-09-01 00:00:00
abstract：:The stimulation of human tumor cells overexpressing epidermal growth factor receptor (EGFR) with EGF enhances tumor development and malignancy. Therefore, compounds that modulate the EGF-mediated signal inducing apoptosis in EGFR-overexpressing cells would represent a new class of antitumor drug and might be useful in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5588
更新日期：2002-09-10 00:00:00
abstract：:The MUC1 mucin is a large membrane-tethered glycoprotein that shows differential expression in many adenocarcinomas, where it contributes to their invasive and metastatic properties. We previously identified DNase I hypersensitive sites at -750 and -250 bp in the human MUC1 gene promoter and showed concordance between...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.04.008
更新日期：2005-08-01 00:00:00
abstract：:Cellular senescence is characterized by a decline in sensitivity to growth factors resulting in cessation of cellular growth. The expression of cellular or viral oncogenes may result in the establishment of cell lines with unlimited proliferative potential ("immortalization"). A variety of viral and cellular oncogenes...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/0014-4827(90)90027-8
更新日期：1990-11-01 00:00:00
abstract：:In chick embryo fibroblasts, the mRNA for extracellular matrix protein tenascin-C is induced 2-fold by cyclic strain (10%, 0.3 Hz, 6 h). This response is attenuated by inhibiting Rho-dependent kinase (ROCK). The RhoA/ROCK signaling pathway is primarily involved in actin dynamics. Here, we demonstrate its crucial impor...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.025
更新日期：2006-05-01 00:00:00
abstract：:Incubation of isolated rat hepatocytes under conditions which support maximal autophagy (amino acid-free medium) caused a marked alteration in the density distribution of lysosomes in continuous metrizamide gradients (mean peak density reduced from 1.14 to 1.09 g/ml). The autophagic sequestration inhibitor 3-methylade...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90141-7
更新日期：1985-04-01 00:00:00
abstract：:Prior studies have shown that synthetic peptides representing the domain of thrombin responsible for high-affinity binding to fibroblasts stimulate chemotactic and cell proliferative signals through a nonproteolytic mechanism. One of these peptides, TP508, has recently been shown to be chemotactic for neutrophils, to ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4372
更新日期：1999-03-15 00:00:00
abstract：:Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/we...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.12.019
更新日期：2011-05-01 00:00:00
abstract：:The replication kinetics and cytological changes of normal human oral keratinocytes (NHOK) isolated from the basal surface of oral epithelial sheet and cultured as dispersed cells in low (0.15 mM) Ca(2+) medium without serum were analyzed. Replicating NHOK were quantitated by cell count and identified by [(3)H]thymidi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4943
更新日期：2000-08-01 00:00:00
abstract：:Pure melanophore populations isolated from the tail skin of the tadpole, Rana catesbeiana, were mass cultured for a period of 2-3 years. All cell lines of amphibian melanophores studied exhibited growth crisis (in vitro ageing) followed by spontaneous transformation to a continuous cell line, as shown by changes in gr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90396-8
更新日期：1983-11-01 00:00:00
abstract：:RalGPS2 is a Ras-independent Guanine Nucleotide Exchange Factor (GEF) for RalA containing a PH domain and an SH3-binding region and it is involved in several cellular processes, such as cytokinesis, control of cell cycle progression, differentiation, cytoskeleton organization and rearrangement. Up to now, few data hav...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.11.036
更新日期：2018-01-15 00:00:00
abstract：:We report here the identification and characterization of STIP, a multi-domain nuclear protein that contains a G-patch, a coiled-coil, and several short tryptophan-tryptophan repeats highly conserved in metazoan species. To analyze their functional role in vivo, we cloned nematode stip-1 genes and determined the spati...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.01.003
更新日期：2007-04-15 00:00:00
abstract：:Lithium has been found to be a novel inhibitor of the terminal differentiation of Friend murine erythroleukemia cells. A general method for the quantitative analysis of differentiation inhibitors has been developed and used to compare the site of inhibition by lithium with that by vanadate. Lithium inhibits the commit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90012-7
更新日期：1987-02-01 00:00:00
abstract：:Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antib...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90289-6
更新日期：1983-08-01 00:00:00
abstract：:Pair formation in the hypotrichous ciliate Euplotes octocarinatus is a poorly understood phenomenon. In order to obtain information about the molecules involved in this process, we compared ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells. Detection of g...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1114
更新日期：1995-04-01 00:00:00
abstract：:Neuroblastoma is a childhood tumor with a poor survival in advanced stage disease despite intensive chemotherapeutic regimes. The new histone deacetylase (HDAC) inhibitor BL1521 has shown promising results in neuroblastoma. Inhibition of HDAC resulted in a decrease in proliferation and metabolic activity, induction of...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.06.024
更新日期：2005-10-01 00:00:00
abstract：:A monoclonal antibody that reacts specifically with the proliferation-sensitive nuclear proteins, isoelectric focusing (IEF) 8Z30 and 8Z31 (molecular weight (MW), 76,000 charge variants, HeLa protein catalogue number) has been characterized. As determined by indirect immunofluorescence, the antibody stains the nucleol...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90011-5
更新日期：1987-02-01 00:00:00
abstract：:Chemoresistance is a severe limitation to glioblastoma (GBM) therapy and there is a strong need to understand the underlying mechanisms that determine its response to different chemotherapeutics. Therefore, we induced resistance in C6 rat glioma cell line, which considerably resembles the characteristics of human GBM....
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.05.018
更新日期：2015-07-15 00:00:00
abstract：:Mip130/LIN-9 is part of a large complex that includes homologs of the Drosophila dREAM (drosophila RB-like, E2F, and Myb) and C. elegans DRM complexes. This complex also includes proteins such as Mip40/LIN-37, Mip120/LIN-54, and LIN-52. In mammalian cells, Mip130/LIN-9 specifically associates with the p107/p130-E2F4 r...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.07.014
更新日期：2009-10-15 00:00:00
abstract：:The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for m...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.111958
更新日期：2020-05-15 00:00:00
abstract：:Cytokeratin 19 (CK19) is widely used as a biomarker for the detection of disseminated tumor cells in blood and bone marrow, and its positivity is considered as an independent prognostication indicator in cancer patients. However, its role in breast cancer progression remains unknown. We had established a stable CK19-e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.02.017
更新日期：2009-07-01 00:00:00
abstract：:The epithelial cell adhesion molecule Ep-CAM is capable of mediating Ca2+-independent homotypic cell-cell adhesion when introduced into cells lacking their own means of cell-cell interactions. We used (confocal) immunofluorescent and (immuno-) electron microscopy to investigate the structural organization of Ep-CAM-me...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4263
更新日期：1999-01-10 00:00:00
abstract：:Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.04.022
更新日期：2006-08-01 00:00:00
abstract：:During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.10.004
更新日期：2007-01-01 00:00:00
abstract：:Signalling from the extracellular matrix (ECM) is a fundamental cellular input that sustains proliferation, opposes cell death and regulates differentiation. Through integrins, cells perceive both the chemical composition and physical properties of the ECM. In particular, cell behaviour is profoundly influenced by the...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2015.10.034
更新日期：2016-04-10 00:00:00
abstract：:Embryonic stem (ES) cells have been tested for potential cell transplantation therapy for CNS disorders. Understanding their differentiation mechanism and identifying factors involved in driving excitatory and inhibitory neuron lineages should enhance the efficacy and efficiency of the cell transplantation therapy. We...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.06.022
更新日期：2006-10-01 00:00:00
