Abstract：
:It is known that freshly dissociated thyroid cell clusters form follicles in suspension culture. Thyroid epithelial cell lines, grown for many generations in vitro, fail to show colloid-containing lumina when cultured as monolayers. Several thyroid cell lines, some transformed, have been tested with respect to their ability to form extracellular lumina when transferred from monolayer to suspension culture. One cell line in particular, the T78 cell line, showed this property when cultured in suspension. Lumina formed within 3 days even in the absence of added thyrotropin (TSH). The ultrastructure of lumina within cell aggregates resembled that of the thyroid follicle in vivo. The ability to undergo morphogenesis may therefore be an intrinsic property of thyroid epithelial cells which is retained for a large number of generations in vitro and is revealed by proper culture conditions. The shift from monolayer to suspension culture may thus lead to the expression of a thyroid differentiated function such as the formation of follicle-like structures.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Nitsch L,Tacchetti C,Tramontano D,Ambesi-Impiombato FSdoi
10.1016/0014-4827(84)90226-xsubject
Has Abstractpub_date
1984-05-01 00:00:00pages
22-30issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(84)90226-Xjournal_volume
152pub_type
杂志文章abstract：:Cross-linking of CD44 in vitro promotes chemokinesis and actin-based dendrite formation in T and B cells. However, the mechanisms by which the adhesion molecule CD44 induces cytoskeleton activation in lymphocytes are still poorly understood. In this study, we have investigated whether myosin isoforms are involved in C...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.002
更新日期：2006-10-15 00:00:00
abstract：:We previously identified a novel centrosomal protein CPAP, which carries a 112-residue motif that is essential for microtubule destabilization. In this report, we define both the microtubule (MT) binding and destabilizing domains in human CPAP and analyze the mutations that affect its MT-destabilizing activity. Analys...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.05.012
更新日期：2008-08-15 00:00:00
abstract：:Human, Chinese hamster or Chinese hamster/human hybrid cytoplasts were fused with UV-irradiated xeroderma pigmentosum group A (XP-A) cells. Unscheduled DNA synthesis (UDS) of the XP-A nucleus was measured 0-2 and 2-4 h after seeding of the fused population. Human cytoplasts did correct the defect in the XP-A nucleus i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90209-6
更新日期：1987-04-01 00:00:00
abstract：:Bax-mediated apoptosis in neurons is involved in many pathologic conditions affecting the central nervous system, including degenerative diseases, stroke, and trauma. Two molecules belonging to the Bcl-2 family, Bcl-2 and Bcl-X(L), protect cells from Bax-induced apoptosis and show distinct expression patterns in adult...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4751
更新日期：2000-02-01 00:00:00
abstract：:Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90209-4
更新日期：1983-09-01 00:00:00
abstract：:Rat myotubes in tissue culture form broad areas of close contact with the substrate. These areas often display two distinct, interdigitating sets of membrane domains. One, the "contact domain", is close to the substrate; the other, termed the "AChR domain", is further from the substrate and is rich in acetylcholine re...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90261-9
更新日期：1989-06-01 00:00:00
abstract：:The exchange of chemical signals between cardiac cells and its relevance for cell communication and metabolic cooperation was reviewed. The role of gap junctions on the transfer of chemical information was discussed as well as the different factors involved in its regulation including changes in cell volume, high gluc...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2016.05.009
更新日期：2016-08-01 00:00:00
abstract：:Several lines of evidence indicate that telomere shortening during in vitro aging of human somatic cells plays a causal role in cellular senescence. A critical telomere length seems to be associated with the replicative block characterizing senescent cells. In this paper we analyzed the mean length of the terminal res...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4398
更新日期：1999-04-10 00:00:00
abstract：:The poor outcome of hepatocellular carcinoma (HCC) is mainly due to the development of fast growth, invasion and metastasis. The role of TET2 has been implicated in some cancer types, but its role and mechanisms in HCC remains elusive. In this study, our findings indicated that TET2 expression frequently increased in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.01.011
更新日期：2018-02-15 00:00:00
abstract：:The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4586
更新日期：1999-09-15 00:00:00
abstract：:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4570
更新日期：1999-08-25 00:00:00
abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.01.017
更新日期：2016-06-10 00:00:00
abstract：:In studies of the ontogeny of fibroblast-epithelial interactions during late fetal lung rat lung development, we have identified two subpopulations of fibroblasts which differed in their ability to promote epithelial cell proliferation or differentiation. As glycosaminoglycans (GAGs) have been implicated in the regula...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90072-g
更新日期：1992-10-01 00:00:00
abstract：:Poly (ADP-ribose) polymerase-1 (PARP1) is a DNA damage sensor that gets activated in proportion to the damage, helping cells to determine whether to repair the damage or initiate cell death processes. We have previously shown PARP1's significance in the developmental processes of Dictyostelium discoideum in addition t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112364
更新日期：2020-12-15 00:00:00
abstract：:Laminin is a major component of the basal lamina upon which all epithelial cells reside in vivo. The synthesis of basal lamina components and their subsequent assembly into a morphologically distinct basal lamina is a differentiated function of epithelial cells in vivo. Ultrastructural studies in our laboratory show t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90036-8
更新日期：1992-11-01 00:00:00
abstract：:TIP48 is a highly conserved eukaryotic AAA+ protein which is an essential cofactor for several complexes involved in chromatin acetylation and remodeling, transcriptional and developmental regulation and nucleolar organization and trafficking. We show that TIP48 abundance in HeLa cells did not change during the cell c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.07.030
更新日期：2005-11-01 00:00:00
abstract：:Skin extracellular matrix (ECM) molecules regulate a variety of cellular activities, including cell movement, which are central to wound healing and metastasis. Regulated cell movement is modulated by proteases and their associated molecules, including the serine proteases urinary-type plasminogen activator (uPA) and ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5644
更新日期：2002-11-01 00:00:00
abstract：:Fluid-phase endocytosis was significantly faster in three lines of human lysosomal storage disease fibroblasts (two I cells and one Sandhoff's) than in three normals. Re-exocytosis of fluid showed similar kinetics in all lines when release was expressed as a percentage of the radioactivity associated with the cells at...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90404-x
更新日期：1984-04-01 00:00:00
abstract：:Epidemiological studies report a negative association between circulating bilirubin concentrations and the risk for cancer and cardiovascular disease. Structurally related tetrapyrroles also possess in vitro anti-genotoxic activity and may prevent mutation prior to malignancy. Furthermore, few data suggest that tetrap...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.12.003
更新日期：2013-02-15 00:00:00
abstract：:A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90438-z
更新日期：1991-12-01 00:00:00
abstract：:In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.01.013
更新日期：2005-05-01 00:00:00
abstract：:Laminins are structurally and functionally major components of the extracellular matrix. Four isoforms of laminins (laminin-1, -2, -5 and -10) are expressed in a specific pattern along the crypt-villus axis of the intestine. Previous works indicated that expression of these isoforms is developmentally regulated and th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.025
更新日期：2005-02-15 00:00:00
abstract：:Rat IPC-81 promyelocytic leukemia cells responded to cAMP analog by undergoing apoptotic cell death both when anchored to fibronectin and when free in the medium. The protein kinase C stimulator 12-O-tetradecanoylphorbol 13-acetate enhanced the anchoring to substratum without impeding cAMP-induced cell death. The immo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1132
更新日期：1993-05-01 00:00:00
abstract：:Alternative splicing is an important mechanism that can disrupt cell cycle control resulting in tumorigenesis. Although many alterations of Transforming Growth Factor Beta (TGFβ) signaling are reported in cancers, the role of splice aberrations in destabilizing this signaling is the least understood mechanism. In this...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.05.004
更新日期：2014-09-10 00:00:00
abstract：:Excision of chromatin loop domains and internucleosomal DNA fragmentation are widely considered as consecutive stages of chromatin disassembly during apoptosis. We report here on apoptosis induced by staurosporine in NB-2a neuroblastoma cells, which was accompanied by excision of chromatin loop domains, but proceeded ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.01.025
更新日期：2007-04-15 00:00:00
abstract：RATIONALE:Vessel formation is a crucial event in tissue repair after injury. Thus, one assumption of innovative therapeutic approaches is the understanding of its molecular mechanisms. Notwithstanding our knowledge of the role of Protein Kinase C epsilon (PKCε) in cardio-protection and vascular restenosis, its role in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.11.011
更新日期：2015-01-15 00:00:00
abstract：:The retention of mRNAs near the nuclei that synthesize them may be an important feature of the organization of multinucleated skeletal myotubes. Here, we assess the possible role of two factors in this localization. First, we examine the role of mRNA half-life, by studying the distribution of the mRNA for the transfer...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3753
更新日期：1997-11-01 00:00:00
abstract：:We have previously established the malignant T lymphoma CS-21 cell line from a spontaneous lymphoma in a BALB/c mouse. CS-21 lymphoma cells grew continuously when they were cocultured with stromal CA-12 cells prepared from lymph nodes. CS-21 lymphoma cells, however, could not proliferate by themselves, and they underw...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1193
更新日期：1993-08-01 00:00:00
abstract：:We have been studying mouse F9 embryonic carcinoma cells which contain no detectable vinculin protein (5.51 cells), and compared them with F9 wild-type cells. Employing atomic force microscopy, we probed the elastic properties of individual F9 wild-type and 5.51 cells by measuring the dynamic response of controlled lo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0223
更新日期：1996-07-10 00:00:00
abstract：:Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors in the urinary system, and its incidence continues to increase. Regulator of calcineurin 1 (RCAN1), one of the genes on chromosome 21, is a crucial mediator of tumor inhibition. RCAN1.4 is best characterized as an endogenous inhibitor o...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.09.017
更新日期：2018-11-15 00:00:00
