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Differential localization of unconventional myosin I and nonmuscle myosin II during B cell spreading.

Abstract：

:Cross-linking of CD44 in vitro promotes chemokinesis and actin-based dendrite formation in T and B cells. However, the mechanisms by which the adhesion molecule CD44 induces cytoskeleton activation in lymphocytes are still poorly understood. In this study, we have investigated whether myosin isoforms are involved in CD44-dependent dendrite formation in activated B cells. Pharmacological inhibition of myosin with 2,3-butanedione monoxime strongly affected spreading and dendrite formation, suggesting that these cellular motors may participate in these phenomena. Furthermore, immunofluorescence analysis showed differences in subcellular localization of class I and class II myosin during B cell spreading. In response to CD44 cross-linking, myosin-1c was polarized to lamellipodia, where F-actin was high. In contrast, the distribution of cytosplasmic nonmuscle class II myosin was not altered. Expressions of myosin-1c and II were also demonstrated in B cells by Western blot. Although the inhibition of PLCgamma, PI3K and MEK-1 activation affected the spreading and dendrite formation in activated B cells, only PLCgamma and MEK-1 inhibition correlated with absence of myosin-1c polarization. Additionally, myosin-1c polarization was observed upon cross-linking of other surface molecules, suggesting a common mechanism for B cell spreading. This work shows that class I and class II myosin are expressed in B cells, are differentially distributed, and may participate in the morphological changes of these cells.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Sumoza-Toledo A,Gillespie PG,Romero-Ramirez H,Ferreira-Ishikawa HC,Larson RE,Santos-Argumedo L

doi

10.1016/j.yexcr.2006.07.002

subject

Has Abstract

pub_date

2006-10-15 00:00:00

pages

3312-22

issue

17

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(06)00276-X

journal_volume

312

pub_type

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