当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Characterization and response of newly developed high-grade glioma cultures to the tyrosine kinase inhibitors, erlotinib, gefitinib and imatinib.

Characterization and response of newly developed high-grade glioma cultures to the tyrosine kinase inhibitors, erlotinib, gefitinib and imatinib.

Abstract：

:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression using epidermal growth factor receptor (EGFR), mutant EGFR (EGFRvIII), platelet derived growth factor receptor (PDGFR), C-Kit and C-Abl together with phosphatase and tensin homolog (PTEN) expression and downstream activation of AKT and phosphorylated ribosomal protein S6 (P70S6K) was analysed in 26 primary glioma cultures treated with the tyrosine kinase inhibitors (TKIs) erlotinib, gefitinib and imatinib. Response to TKIs was assessed using 50% inhibitory concentrations (IC(50)). Response for each culture was compared with the EGFR/PDGFR immunocytochemical pathway profile using hierarchical cluster analysis (HCA) and principal component analysis (PCA). Erlotinib response was not strongly associated with high expression of the growth factor pathway components. PTEN expression did not correlate with response to any of the three TKIs. Increased EGFR expression was associated with gefitinib response; increased PDGFR-α expression was associated with imatinib response. The results of this in vitro study suggest gefitinib and imatinib may have therapeutic potential in HGG tumours with a corresponding growth factor receptor expression profile.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

doi

10.1016/j.yexcr.2012.01.014

subject

Has Abstract

pub_date

2012-03-10 00:00:00

pages

641-52

issue

5

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(12)00020-1

journal_volume

318

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • NF-κB mediated miR-130a modulation in lung microvascular cell remodeling: Implication in pulmonary hypertension.

    abstract：:Pulmonary arterial hypertension (PAH) is a progressive pulmonary vascular disease which is associated with pulmonary arterial endothelial cells (PAEC) dysfunction and pulmonary arterial smooth muscle cells proliferation. Moreover, inflammation is contributing a critical role in EC dysfunction and remains elusive. Nucl...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.07.024

    authors： Li L,Kim IK,Chiasson V,Chatterjee P,Gupta S

    更新日期：2017-10-01 00:00:00

  • Aurora A regulates the architecture of the Golgi apparatus.

    abstract：:The Golgi apparatus plays roles in cell polarity, directional cell migration, and bipolar spindle assembly, as well as the secretary pathway. In addition, recent studies have suggested the Golgi-dependent control of mitotic entry. We studied the role of the centrosomal kinase Aurora A in maintaining the Golgi apparatu...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.03.024

    authors： Kimura M,Takagi S,Nakashima S

    更新日期：2018-06-01 00:00:00

  • The cytochrome b5 tail anchors and stabilizes subdomains of human DNA topoisomerase II alpha in the cytoplasm of retrovirally infected mammalian cells.

    abstract：:DNA topoisomerase II (topo II) is the target of many anticancer drugs and is often altered in drug-resistant cell lines. In some tumor cell lines truncated isoforms of topo IIalpha are localized to the cytoplasm. To study the localization and function of individual enzyme domains, we have epitope-tagged several fragme...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4446

    authors： Soltermann A,Ernst A,Leroy D,Stahel RA,Gasser SM

    更新日期：1999-06-15 00:00:00

  • siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.

    abstract：:A large number of intracellular proteins are degraded by the ubiquitin-proteasome system, one of the major protein degradation pathways. It produces peptides of several different sizes through protein degradation, and these peptides are rapidly degraded into free amino acids by various intracellular aminopeptidases. P...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112337

    authors： Osana S,Kitajima Y,Suzuki N,Xu Y,Murayama K,Nagatomi R

    更新日期：2020-12-01 00:00:00

  • RelA/NF-kappaB transcription factor associates with alpha-actinin-4.

    abstract：:The NF-kappaB/RelA family of transcription factors regulates inducible transcription of a large number of genes in response to diverse stimuli. Little is known, however, about the location of NF-kappaB in the cytoplasm and the transport mechanism to the nucleus. We found that NF-kappaB is associated with the actin-bin...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.12.001

    authors： Babakov VN,Petukhova OA,Turoverova LV,Kropacheva IV,Tentler DG,Bolshakova AV,Podolskaya EP,Magnusson KE,Pinaev GP

    更新日期：2008-03-10 00:00:00

  • An antigen expressed in proliferating cells at late G1-S phase.

    abstract：:A monoclonal antibody Pr-28 was prepared, which recognized an antigen present only in proliferating cells. Immunofluorescence analysis of Pr-28 antigen showed that the antigen was localized mainly in perinuclear cytoplasm. Although Pr-28 antibody was produced against a chicken cell antigen, it reacts not only with chi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90312-0

    authors： Matsuhashi S,Watanabe T,Hori K

    更新日期：1987-06-01 00:00:00

  • A high-affinity binding protein for the regulatory subunit of cAMP-dependent protein kinase II in the centrosome of human cells.

    abstract：:In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for human regulatory subunits RII alpha RII beta of the cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies with several antibodies directed against RII alpha and RII beta show that these ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1029

    authors： Keryer G,Rios RM,Landmark BF,Skalhegg B,Lohmann SM,Bornens M

    更新日期：1993-02-01 00:00:00

  • Accumulation of autolysosomes after receptor-mediated introduction of Ep459-asialofetuin conjugate into lysosomes in rat hepatocytes.

    abstract：:Administration of Ep459-asialofetuin conjugate (Ep459-AF) and pepstatin-asialofetuin conjugate (Ps-AF) to rats effectively inhibited lysosomal BANA hydrolase and cathepsin D in the liver, respectively, at a very low dose. Ep459-AF treatment also led to an accumulation of autolysosomes in rat liver. There was a close c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90257-5

    authors： Furuno K,Miwa N,Ishikawa T,Kato K

    更新日期：1985-01-01 00:00:00

  • Hsf1 on a leash - controlling the heat shock response by chaperone titration.

    abstract：:Heat shock factor 1 (Hsf1) is an ancient transcription factor that monitors protein homeostasis (proteostasis) and counteracts disturbances by triggering a transcriptional programme known as the heat shock response (HSR). The HSR is transiently activated and upregulates the expression of core proteostasis genes, inclu...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2020.112246

    authors： Masser AE,Ciccarelli M,Andréasson C

    更新日期：2020-11-01 00:00:00

  • Inhibitors of phosphoinositide 3-kinase cause defects in the postendocytic sorting of beta2-adrenergic receptors.

    abstract：:Phosphatidylinositol 3-kinase inhibitors have been shown to affect endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated beta(2)-adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.04.034

    authors： Awwad HO,Iyer V,Rosenfeld JL,Millman EE,Foster E,Moore RH,Knoll BJ

    更新日期：2007-07-15 00:00:00

  • Protein kinase calpha is an effector of hexamethylene bisacetamide-induced differentiation of Friend erythroleukemia cells.

    abstract：:The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4312

    authors： Mallia CM,Aguirre V,McGary E,Tang Y,Scandurro AB,Liu C,Noguchi CT,Beckman BS

    更新日期：1999-02-01 00:00:00

  • Correlation between torsion and streaming in Physarum.

    abstract：:Experiments are described which show that the torsional oscillations arising in a strand of the slime mould, Physarum polycephalum, under certain conditions remain closely correlated to oscillations in cytoplasmic streaming. When this is the case, the direction of rotation is related to the direction of streaming by a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90401-9

    authors： Obendorf P,O'Sullivan RA

    更新日期：1983-11-01 00:00:00

  • Cell-scaffold interaction within engineered tissue.

    abstract：:The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.02.028

    authors： Chen H,Liu Y,Jiang Z,Chen W,Yu Y,Hu Q

    更新日期：2014-05-01 00:00:00

  • Calpains: markers of tumor aggressiveness?

    abstract：:Rhabdomyosarcoma (RMS) are soft-tissue sarcoma commonly encountered in childhood. RMS cells can acquire invasive behavior and form metastases. The metastatic dissemination implicates many proteases among which are mu-calpain and m-calpain. Study of calpain expression and activity underline the deregulation of calpain ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.02.017

    authors： Roumes H,Leloup L,Dargelos E,Brustis JJ,Daury L,Cottin P

    更新日期：2010-05-15 00:00:00

  • Intracellular trafficking and degradation of unassociated proalpha2 chains of collagen type I.

    abstract：:Procollagen I is a trimer consisting of two proalpha1(I) chains and one proalpha 2(I) chain. In certain cases of mild osteogenesis imperfecta, abnormal proalpha1(I) chains are degraded very soon after synthesis. As a consequence, the cells produce excess proalpha2(I) chains, which cannot form trimers and are not secre...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.01.029

    authors： Gotkin MG,Ripley CR,Lamande SR,Bateman JF,Bienkowski RS

    更新日期：2004-06-10 00:00:00

  • VE-Cadherin mediates endothelial cell capillary tube formation in fibrin and collagen gels.

    abstract：:Various cell adhesion molecules mediate the diverse functions of the vascular endothelium, such as cell adhesion, neutrophil migration, and angiogenesis. In order to identify cell adhesion molecules important for angiogenesis, we used an in vitro model (Chalupowicz, Chowdhury, Bach, Barsigian, and Martinez, J. Cell Bi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3844

    authors： Bach TL,Barsigian C,Chalupowicz DG,Busler D,Yaen CH,Grant DS,Martinez J

    更新日期：1998-02-01 00:00:00

  • In vitro replication and differentiation of normal human oral keratinocytes.

    abstract：:The replication kinetics and cytological changes of normal human oral keratinocytes (NHOK) isolated from the basal surface of oral epithelial sheet and cultured as dispersed cells in low (0.15 mM) Ca(2+) medium without serum were analyzed. Replicating NHOK were quantitated by cell count and identified by [(3)H]thymidi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4943

    authors： Kang MK,Bibb C,Baluda MA,Rey O,Park NH

    更新日期：2000-08-01 00:00:00

  • Cyclic AMP in ovarian cancer cells both inhibits proliferation and increases c-KIT expression.

    abstract：:C-KIT encodes a tyrosine kinase receptor (KIT) that, when activated by its ligand (KL), stimulates proliferation, differentiation, migration, and survival. Greater than 70% of epithelial ovarian cancers coexpress c-KIT and KL. C-KIT and KL expression levels have been shown to be up-regulated by cAMP in some cell types...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5426

    authors： Shaw TJ,Keszthelyi EJ,Tonary AM,Cada M,Vanderhyden BC

    更新日期：2002-02-01 00:00:00

  • Differences in tyrosine phosphorylation of oocyte key proteins during 5HT-induced meiosis reinitiation in two bivalve species.

    abstract：:The neurohormone serotonin (5HT) triggers meiosis reinitiation in oocytes of the pelecypod molluscs Spisula solidissima and Ruditapes philippinarum. However, while Spisula oocytes complete maturation, Ruditapes oocytes arrest in metaphase I. Anti-phosphotyrosine antibody revealed that serotonin triggered an early tyro...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1163

    authors： Abdelmajid H,Rivailler P,Krantic S,Guerrier P

    更新日期：1994-06-01 00:00:00

  • Purification and characterization of integrin alpha 9 beta 1.

    abstract：:A new beta 1-containing integrin was isolated from rat liver by affinity chromatography on Sepharose conjugated with the peptide GRGDSPC. The interaction was weakened but not abolished when the arginine and/or aspartic acid in the peptide were replaced with lysine and glutamic acid, respectively. In contrast, the cyst...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1189

    authors： Forsberg E,Ek B,Engström A,Johansson S

    更新日期：1994-07-01 00:00:00

  • Taxol protects the microtubules of concanavalin A-activated lymphocytes from disassembly by methylmercury, but DNA synthesis is still inhibited.

    abstract：:We have shown previously that there is a good correlation between the degree of microtubule disassembly by methylmercury (MeHg) and the extent of inhibition of DNA replication in Concanavalin A (Con A)-stimulated mouse splenic lymphocytes. The purpose of this study was to determine if these two events are causally rel...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90383-6

    authors： Roy C,Prasad KV,Reuhl KR,Little JE,Valentine BK,Brown DL

    更新日期：1991-08-01 00:00:00

  • Induction of hyper-adhesion attenuates autoimmune-induced keratinocyte cell-cell detachment and processing of adhesion molecules via mechanisms that involve PKC.

    abstract：:In confluent keratinocyte monolayers, desmosomal adhesion gradually becomes calcium-independent and this is associated with an increase in the strength of intercellular adhesion (hyper-adhesion). In this study, we investigated the functional and molecular significance of hyper-adhesion in a system challenged by autoim...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.10.005

    authors： Cirillo N,Lanza A,Prime SS

    更新日期：2010-02-15 00:00:00

  • Regulation of microphthalmia-associated transcription factor MITF protein levels by association with the ubiquitin-conjugating enzyme hUBC9.

    abstract：:The basic helix-loop-helix/leucine zipper (bHLH/ZIP) microphthalmia-associated transcription factor (MITF) regulates transcription of genes encoding enzymes essential for melanin biosynthesis in melanocytes and retinal pigmented epithelial cells. To determine how MITF activity is regulated, we used the yeast two-hybri...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4803

    authors： Xu W,Gong L,Haddad MM,Bischof O,Campisi J,Yeh ET,Medrano EE

    更新日期：2000-03-15 00:00:00

  • The impact of maternal serum on development of enolase activity in fetal rat brain cell culture.

    abstract：:The effect of gestational age on serum-mediated changes in enolase activity was tested in a fetal rat brain cell culture. After 6 days exposure to graded concentrations (10 and 20%) of nonpregnant female rat sera, enolase activity in brain cell cultures increased from 2.83 +/- 0.03 to 3.74 +/- 0.19 mumol/min/mg protei...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90298-o

    authors： Shambaugh GE 3rd,Clough D,Munari L,Koehler RR,Radosevich JA

    更新日期：1990-02-01 00:00:00

  • Microinjected anti-actin antibodies decrease gap junctional intercellular commmunication in cultured astrocytes.

    abstract：:The purpose of the present study was to investigate the influence of the actin cytoskeleton on gap junctional intercellular communication (GJIC) in cultured astrocytes. This report describes an decrease of GJIC following microinjection of anti-actin antibodies or cytochalasin D treatment. Dye transfer of microinjected...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5652

    authors： Theiss C,Meller K

    更新日期：2002-12-10 00:00:00

  • Long noncoding RNA CCAT2 promotes hepatocellular carcinoma proliferation and metastasis through up-regulation of NDRG1.

    abstract：BACKGROUND:Emerging studies demonstrate that long noncoding RNAs (lncRNAs) play crucial roles in hepatocarcinogenesis through various mechanisms. LncRNA CCAT2 was a newly discovered lncRNA and amplified in several cancers. However, the mechanisms involved in function of CCAT2 in hepatocellular carcinoma (HCC) remain to...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.03.029

    authors： Liu Y,Wang D,Li Y,Yan S,Dang H,Yue H,Ling J,Chen F,Zhao Y,Gou L,Tang P,Huang A,Tang H

    更新日期：2019-06-01 00:00:00

  • Osteopontin is associated with nuclear factor kappaB gene expression during tail-suspension-induced bone loss.

    abstract：:Osteoporosis due to unloading-induced bone loss is a critical issue in the modern aging society. Although the mechanisms underlying this phenomenon are largely unknown, osteopontin (OPN) is one of the critical mediators required for unloading-induced bone loss [M. Ishijima, S.R. Rittling, T. Yamashita, K. Tsuji, H. Ku...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.06.003

    authors： Ishijima M,Ezura Y,Tsuji K,Rittling SR,Kurosawa H,Denhardt DT,Emi M,Nifuji A,Noda M

    更新日期：2006-10-01 00:00:00

  • Betulinic acid derivatives can protect human Müller cells from glutamate-induced oxidative stress.

    abstract：:Müller cells are the predominant retinal glial cells. One of the key roles of Müller cells is in the uptake of the neurotransmitter glutamate and in its conversion to glutamine. Müller cell dysfunction due to oxidative stress elicited by high glutamate concentrations can lead to toxicity, which promote the pathogenesi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111509

    authors： Cheng Z,Zhang T,Zheng J,Ding W,Wang Y,Li Y,Zhu L,Murray M,Zhou F

    更新日期：2019-10-01 00:00:00

  • A syndecan-4 binding peptide derived from laminin 5 uses a novel PKCε pathway to induce cross-linked actin network (CLAN) formation in human trabecular meshwork (HTM) cells.

    abstract：:In this study, we examined the role(s) of syndecan-4 in regulating the formation of an actin geodesic dome structure called a cross-linked actin network (CLAN) in which syndecan-4 has previously been localized. CLANs have been described in several different cell types, but they have been most widely studied in human t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.07.035

    authors： Filla MS,Clark R,Peters DM

    更新日期：2014-10-01 00:00:00

  • The protein composition of the nuclear matrix of murine P19 embryonal carcinoma cells is differentiation-stage dependent.

    abstract：:The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90072-4

    authors： Stuurman N,Van Driel R,De Jong L,Meijne AM,Van Renswoude J

    更新日期：1989-02-01 00:00:00