Abstract:
:A new beta 1-containing integrin was isolated from rat liver by affinity chromatography on Sepharose conjugated with the peptide GRGDSPC. The interaction was weakened but not abolished when the arginine and/or aspartic acid in the peptide were replaced with lysine and glutamic acid, respectively. In contrast, the cysteine was necessary for binding of the integrin. The beta 1-associated protein, referred to as alpha 9, had an N-terminal amino acid sequence related to but distinct from previously described integrin alpha-subunits. In addition, an internal peptide sequence was obtained which confirmed that the protein is a new member of the family of integrin alpha-subunits. An antiserum raised against a synthetic peptide corresponding to amino acids 1-16 of alpha 9 reacted specifically with this protein and was used to identify alpha 9 in several tissues. The integrin alpha 9 beta 1 was not retained on Sepharose conjugated with Englebreth-Holm-Swarm tumor (EHS)-laminin, collagen type I, or a 105-kDa cell-binding fragment of fibronectin. However, it did bind specifically to EHS-laminin and collagen type I adsorbed to plastic microtiter wells. The sites of the interactions were localized to fragment E8 of EHS-laminin and to cyanogen bromide fragment 8 of collagen alpha 1(I) and were not inhibited by soluble RGD-containing peptides. The results indicate that alpha 9 beta 1 is a widely distributed laminin/collagen receptor which may have additional, yet unidentified ligands.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Forsberg E,Ek B,Engström A,Johansson Sdoi
10.1006/excr.1994.1189subject
Has Abstractpub_date
1994-07-01 00:00:00pages
183-90issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(84)71189-Xjournal_volume
213pub_type
杂志文章abstract::The rheumatic diseases are characterized by the production of autoantibodies that are usually directed against components of the cell nucleus. In this communication, we describe autoantibodies that recognize DNA topoisomerase II (anti-topoII) present in the serum of a patient with systemic lupus erythematosus. Several...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90067-0
更新日期:1989-02-01 00:00:00
abstract::In this study, we examined the role(s) of syndecan-4 in regulating the formation of an actin geodesic dome structure called a cross-linked actin network (CLAN) in which syndecan-4 has previously been localized. CLANs have been described in several different cell types, but they have been most widely studied in human t...
journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2014.07.035
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
abstract::Autosomal dominant polycystic kidney disease (ADPKD), a hereditary renal disease caused by mutations in PKD1 (85%) or PKD2 (15%), is characterized by the development of gradually enlarging multiple renal cysts and progressive renal failure. Polycystin-1 (PC1), PKD1 gene product, is an integral membrane glycoprotein wh...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Hematopoiesis is the hierarchical process in which all lineages of blood cells are produced by self-renewing hematopoietic stem cells (HSCs) in the bone marrow (BM). While the regulatory factors that maintain proper HSC function and lineage output under normal conditions are well understood, significantly less is know...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
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abstract::Previous work has suggested that adhesive or cytoskeletal structures alter during epithelial transformation. The purpose of the present studies was to see whether such reorganizations were accompanied by consistent changes in the biophysical characteristics of cells. We used in vitro cell lines from rat liver and resp...
journal_title:Experimental cell research
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doi:10.1016/0014-4827(87)90232-1
更新日期:1987-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1311
更新日期:1995-09-01 00:00:00
abstract::We have studied the cytotoxicity of ricin in cells treated with brefeldin A (BFA), which dramatically disrupts the structure of the Golgi apparatus causing Golgi content and membrane to redistribute to the ER. BFA inhibits the cytotoxicity of ricin in Chinese hamster ovary, normal rat kidney, and Vero cells and abolis...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90056-z
更新日期:1991-02-01 00:00:00
abstract::Rat glomerular epithelial cells were grown to confluency on semipermeable tissue culture inserts and the plasminogen system of these cells was analyzed using enzyme assays, Western analysis, zymography, and reverse transcriptase-PCR. The glomerular epithelial cells are capable of activating exogenous plasminogen to pl...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1191
更新日期:1995-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90220-5
更新日期:1990-01-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.04.006
更新日期:2008-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.11.016
更新日期:2017-01-01 00:00:00
abstract::Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90495-7
更新日期:1986-10-01 00:00:00
abstract::Human neutrophils developed long thin tubulovesicular extensions (cytonemes) upon adhesion to fibronectin-coated substrata, when spreading was blocked. We observed extension formation when neutrophils were plated to fibronectin-coated substrata in Na(+)-free extracellular medium or in the presence of drugs capable of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5227
更新日期:2001-06-10 00:00:00
abstract::The mechanisms of replicative senescence by telomere shortening are not fully understood. The Indian muntjac has the fewest chromosomes of all mammals, greatly simplifying the analysis of each telomere over time. In this study, telomere shortening was observed throughout the life span of cultured normal muntjac cells ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5645
更新日期:2002-11-15 00:00:00
abstract::SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90692-x
更新日期:1984-09-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5285
更新日期:2001-08-15 00:00:00
abstract::JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.04.021
更新日期:2009-07-15 00:00:00
abstract::Rap proteins (Rap1, Rap2a, b, c) are small molecular weight GTPases of the Ras family. Rap G proteins mediate diverse cellular events such as cell adhesion, proliferation, and gene activation through various signaling pathways. Activation of Rap signal is regulated tightly by several specific regulatory proteins inclu...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2013.04.009
更新日期:2013-09-10 00:00:00
abstract::Protein synthesis by Polyspondylium violaceum in response to the chemoattractant glorin was analyzed by two-dimensional gel electrophoresis to determine if glorin can affect gene expression. When cells developing in a shaking suspension culture were given glorin, five proteins exhibited significantly increased and thr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90323-3
更新日期:1990-02-01 00:00:00
abstract::Defects in the regulation of programmed cell death play a fundamental role in the development of neoplasia and neurological disorders, both of which are linked to the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection. We previously showed that the HTLV-1 Tax protein protects from apoptosis induced by seru...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.01.009
更新日期:2006-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.06.025
更新日期:2008-01-01 00:00:00
abstract::Primary cultures of renal rabbit proximal tubule cells were initiated from a pure suspension of proximal tubule fragments. Proximal tubule cells were grown in a hormone-supplemented, serum-free medium containing low concentrations of antibiotics. Confluent monolayers exhibited multicellular dome formation, indicating ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90123-c
更新日期:1991-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1158
更新日期:1993-07-01 00:00:00
abstract::Bax∆2 is a functional pro-apoptotic Bax isoform having alterations in its N-terminus, but sharing the rest of its sequence with Baxα. Bax∆2 is unable to target mitochondria due to the loss of helix α1. Instead, it forms cytosolic aggregates and activates caspase 8. However, the functional domain(s) responsible for Bax...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.08.016
更新日期:2017-10-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5118
更新日期:2001-02-15 00:00:00
abstract::Epithelial ovarian cancer is the most common form of gynaecological malignancy. This lethal disease is thought to arise in ovarian surface epithelial (OSE) cells. The biology of these cells is not well understood, due to the limited amount of tissue that can be obtained from a single biopsy and their limited life span...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00218-0
更新日期:2003-08-15 00:00:00
abstract::There are a number of low-abundance small nuclear RNAs (snRNAs) in eukaryotic cells. Many of them have been assigned functions in the biogenesis of cellular RNAs, such as splicing and 3' end processing. Here, we present the sequence of Xenopus U12 snRNA and compare the secondary structures of the low-abundance U11 and...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0372
更新日期:1996-12-15 00:00:00
abstract::Poly (ADP-ribose) polymerase-1 (PARP1) is a DNA damage sensor that gets activated in proportion to the damage, helping cells to determine whether to repair the damage or initiate cell death processes. We have previously shown PARP1's significance in the developmental processes of Dictyostelium discoideum in addition t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2020.112364
更新日期:2020-12-15 00:00:00
abstract::Signal-mediated nuclear import was investigated in proliferating BALB/c 3T3 cells 1, 2, 4, 6, 8, 12, 18, and 21 h after the onset of anaphase. Using nucleoplasmin-coated colloidal gold particles to assay transport capacity, it was found that import was greatest at 1 h postanaphase (after complete reformation of the nu...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1333
更新日期:1994-11-01 00:00:00