当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > A syndecan-4 binding peptide derived from laminin 5 uses a novel PKCε pathway to induce cross-linked actin network (CLAN) formation in human trabecular meshwork (HTM) cells.

A syndecan-4 binding peptide derived from laminin 5 uses a novel PKCε pathway to induce cross-linked actin network (CLAN) formation in human trabecular meshwork (HTM) cells.

Abstract：

:In this study, we examined the role(s) of syndecan-4 in regulating the formation of an actin geodesic dome structure called a cross-linked actin network (CLAN) in which syndecan-4 has previously been localized. CLANs have been described in several different cell types, but they have been most widely studied in human trabecular meshwork (HTM) cells where they may play a key role in controlling intraocular pressure by regulating aqueous humor outflow from the eye. In this study we show that a loss of cell surface synedcan-4 significantly reduces CLAN formation in HTM cells. Analysis of HTM cultures treated with or without dexamethasone shows that laminin 5 deposition within the extracellular matrix is increased by glucocorticoid treatment and that a laminin 5-derived, syndecan-4-binding peptide (PEP75), induces CLAN formation in TM cells. This PEP75-induced CLAN formation was inhibited by heparin and the broad spectrum PKC inhibitor Ro-31-7549. In contrast, the more specific PKCα inhibitor Gö 6976 had no effect, thus excluding PKCα as a downstream effector of syndecan-4 signaling. Analysis of PKC isozyme expression showed that HTM cells also expressed both PKCγ and PKCε. Cells treated with a PKCε agonist formed CLANs while a PKCα/γ agonist had no effect. These data suggest that syndecan-4 is essential for CLAN formation in HTM cells and that a novel PKCε-mediated signaling pathway can regulate formation of this unique actin structure.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Filla MS,Clark R,Peters DM

doi

10.1016/j.yexcr.2014.07.035

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

171-82

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(14)00326-7

journal_volume

327

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing.

    abstract：:We have reported that PAP-1, a product of a causative gene for autosomal retinitis pigmentosa, plays a role in splicing. In this study, CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor, was identified as a PAP-1 binding protein and its function as ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.10.012

    authors： Maita H,Kitaura H,Ariga H,Iguchi-Ariga SM

    更新日期：2005-02-15 00:00:00

  • Isolation of an MSP1-derived transcriptionally active nucleolar particle.

    abstract：:A discrete macromolecular structure with inherent transcription activity has been isolated from Novikoff nucleoli. The particle (350 A diameter) was released from the intact nucleoli by digestion with restriction endonuclease (Msp1) and is enriched in repetitive DNA sequences both 5' and 3' to the ribosomal genes. In ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90531-8

    authors： Rosenberg NL

    更新日期：1986-07-01 00:00:00

  • Screening of genes involved in cell migration in Dictyostelium.

    abstract：:A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.12.002

    authors： Nagasaki A,Uyeda TQ

    更新日期：2008-03-10 00:00:00

  • Signal transduction in endothelial cells by the angiogenesis inhibitor histidine-rich glycoprotein targets focal adhesions.

    abstract：:Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.04.022

    authors： Lee C,Dixelius J,Thulin A,Kawamura H,Claesson-Welsh L,Olsson AK

    更新日期：2006-08-01 00:00:00

  • p66Shc promotes HCC progression in the tumor microenvironment via STAT3 signaling.

    abstract：:The development of hepatocellular carcinoma (HCC) is strongly associated with chronic inflammation. p66Shc is an oxidase previously shown to promote androgen-independent cell growth through generation of reactive oxygen species. However, the importance and biologic functions of p66Shc in HCC are unclear. The clinical ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111550

    authors： Huang P,Feng X,Zhao Z,Yang B,Fang T,Guo M,Xia J

    更新日期：2019-10-15 00:00:00

  • Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color.

    abstract：:Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated nucleotides has been recently proposed as a means of analyzing DNA replication and detecting proliferating cells. The presence of numerous in situ DNA strand breaks is also an inherent feature of apoptotic cells. T...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0004

    authors： Li X,Melamed MR,Darzynkiewicz Z

    更新日期：1996-01-10 00:00:00

  • Detection of the ATP-dependent nonmitochondrial calcium store in a cell surface-derived vesicle fraction from isolated rat hepatocytes.

    abstract：:In preceding studies, the IP3-sensitive Ca2+ store of the hamster insulinoma cell line, HIT, was detected in cell surface protrusions such as microvilli and related membrane structures [Lange, K., and Brandt, U. (1993) FEBS Lett. 320, 183-188; and (1993) FEBS Lett. 325, 205-209]. In this study, these experiments were ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0316

    authors： Lange J,Schlieps K,Lange K,Brandt U,Knoll-Köhler E

    更新日期：1996-11-01 00:00:00

  • TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner.

    abstract：:Satellite cells are a quiescent heterogeneous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-beta (TGF-beta) superfamily members are elevated post-injury and their importance in the r...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.10.037

    authors： Schabort EJ,van der Merwe M,Loos B,Moore FP,Niesler CU

    更新日期：2009-02-01 00:00:00

  • Slit/Robo1 signaling regulates neural tube development by balancing neuroepithelial cell proliferation and differentiation.

    abstract：:Formation of the neural tube is the morphological hallmark for development of the embryonic central nervous system (CNS). Therefore, neural tube development is a crucial step in the neurulation process. Slit/Robo signaling was initially identified as a chemo-repellent that regulated axon growth cone elongation, but it...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.02.011

    authors： Wang G,Li Y,Wang XY,Han Z,Chuai M,Wang LJ,Ho Lee KK,Geng JG,Yang X

    更新日期：2013-05-01 00:00:00

  • Phosphoinositide 3-kinase regulates the role of retromer in transcytosis of the polymeric immunoglobulin receptor.

    abstract：:Retromer is a multimeric protein complex that mediates intracellular receptor sorting. One of the roles of retromer is to promote transcytosis of the polymeric immunoglobulin receptor (pIgR) and its ligand polymeric immunoglobulin A (pIgA) in polarized epithelial cells. In Madin-Darby Canine Kidney (MDCK) cells, overe...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.11.010

    authors： Vergés M,Sebastián I,Mostov KE

    更新日期：2007-02-15 00:00:00

  • Cardiac apoptosis caused by elevated cholesterol level in experimental autoimmune myocarditis.

    abstract：:It has been reported that cholesterol-lowing agents can ameliorate severity of myocarditis. However, the beneficial effect of the agents has been claimed to be independent of cholesterol reduction as there is no significant change in the plasma cholesterol level in myocarditis. In the present study, using experimental...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112169

    authors： Chang H,Wang Y,Wu Y,Ma P,Song Y,Liu C,Ye Y,Qi JH,Qi Z

    更新日期：2020-10-01 00:00:00

  • AKT upregulates B-Raf Ser445 phosphorylation and ERK1/2 activation in prostate cancer cells in response to androgen depletion.

    abstract：:Upregulated ERK1/2 activity is often correlated with AKT activation during prostate cancer (PCa) progression, yet their functional relation needs elucidation. Using androgen-deprived LNCaP cells, in which ERK1/2 activation occurs in strong correlation with AKT activation, we found that AKT-mediated B-Raf regulation is...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.008

    authors： Hong SK,Jeong JH,Chan AM,Park JI

    更新日期：2013-07-15 00:00:00

  • Do IL-3/GM-CSF effect on the myofibroblastic differentiation of human adipose derived stromal cells?

    abstract：BACKGROUND:Capsular contracture is an incurable complication after silicone-based implant surgery. Myofibroblast is the predominant cell in the contracted capsule. We hypothesized that human adipose derive stromal cells (hASCs) together with fibroblast may show a similar phenotypic characteristics of myofibroblast afte...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.03.056

    authors： Lee JS,Tae SS,Kim DY,Han SK,Kim WK,Dhong ES

    更新日期：2017-06-15 00:00:00

  • Cytoskeleton protein 4.1R suppresses murine keratinocyte cell hyperproliferation via activating the Akt/ERK pathway in an EGFR-dependent manner.

    abstract：:The correct functioning of epidermal growth factor receptor (EGFR), a receptor tyrosine kinase, is required for normal skin development and homeostasis. Cellular hyperproliferation induced by dysregulation of EGFR is tightly associated with structural and functional defects of hair follicles, skin lesions, and tumorig...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111648

    authors： Chen L,Wang T,Ji X,Ding C,Liang T,Liu X,Lu J,Guo X,Kang Q,Ji Z

    更新日期：2019-11-15 00:00:00

  • Repopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro. A novel system for type II pneumocyte culture.

    abstract：:This paper describes the preparation of lung acellular alveolar matrix fragments and culture of rat type II pneumocytes directly on the alveolar epithelial basement membrane, thereby permitting study of the effect of lung basement membrane on the morphology and function of type II cells. Collagen types I, III, IV and ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90347-2

    authors： Lwebuga-Mukasa JS,Ingbar DH,Madri JA

    更新日期：1986-02-01 00:00:00

  • Micromanipulation of mitotic chromosomes in PTK2 cells using laser-induced optical forces ("optical tweezers").

    abstract：:To study the potential use of optical forces to manipulate chromosome movement, we have used a Nd:YAG laser at a wavelength of 1.06 microns focused into a phase contrast microscope. Metaphase and anaphase chromosomes were exposed while being monitored by video microscopy. The results indicated that when optical forces...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90475-a

    authors： Liang H,Wright WH,He W,Berns MW

    更新日期：1991-11-01 00:00:00

  • Characterization of glucocerebrosidase in peripheral blood cells and cultured blastoid cells.

    abstract：:We have characterized glucocerebrosidase in various cell types of peripheral blood of control subjects and in cultured human blastoid cells. The intracellular level of glucocerebrosidase in cultured blastoid cells (10-30 nmol substrate hydrolyzed/h.mg protein) resembles closely values observed for leukocyte cell types...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90472-7

    authors： Aerts JM,Heikoop J,van Weely S,Donker-Koopman WE,Barranger JA,Tager JM,Schram AW

    更新日期：1988-08-01 00:00:00

  • Isolation and immunohistochemical characterization of two developmentally regulated highly insoluble antigens from the membrane of sinusoidal rat liver cells.

    abstract：:Using sequential extractions with buffers containing or not containing neutral detergent, two highly insoluble protein components were found in livers of 30-day-old rats. These compounds (molecular weights (MW) 52 900 and 33 200 respectively) were practically absent from livers of young rats (between 5 and 8 postnatal...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90197-6

    authors： Zanetta JP,Bingen A,Dontenwill M,Vincendon G

    更新日期：1984-12-01 00:00:00

  • Characterization and physiological importance of a novel cell cycle regulated protein kinase in Xenopus laevis oocytes that phosphorylates cyclin B2.

    abstract：:We have partially purified a specific cyclin B2 kinase (cyk) from prophase oocytes of Xenopus laevis after an ATP-gamma-S activation step. Phosphopeptide analysis identified Ser53 as the major in vitro phosphorylation site for cyk in cyclin B2. Using a synthetic peptide derived from cyclin B2 encompassing Ser53 (cykti...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3436

    authors： Derua R,Stevens I,Waelkens E,Fernandez A,Lamb N,Merlevede W,Goris J

    更新日期：1997-02-01 00:00:00

  • Commitment of 3T3-F442A cells to adipocyte differentiation takes place during the first 24-36 h after adipogenic stimulation: TNF-alpha inhibits commitment.

    abstract：:We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(02)00036-8

    authors： Castro-Muñozledo F,Beltrán-Langarica A,Kuri-Harcuch W

    更新日期：2003-04-01 00:00:00

  • Cellular and cytoskeleton morphology and strength of adhesion of cells on self-assembled monolayers of organosilanes.

    abstract：:The objective of this study was to explore the potential use of self-assembled monolayers (SAMs) of alkylamine and arylalkyamine as well-defined, homogeneous, tailored in vitro model surfaces for exploring the effect of hydrodynamic flow on morphology and strength of adhesion of human umbilical vein endothelial cells....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4156

    authors： Kapur R,Rudolph AS

    更新日期：1998-10-10 00:00:00

  • EAT/mcl-1 expression in the human embryonal carcinoma cells undergoing differentiation or apoptosis.

    abstract：:Differentiation and apoptosis are precisely regulated events in early embryogenesis. Retinoic acid-induced differentiation in the embryonal carcinoma (EC) cell line NCR-G3 triggers concurrent induction of apoptosis. Using this system, which serves as a model of early embryogenesis, the expression of various bcl-2-rela...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5203

    authors： Sano M,Umezawa A,Abe H,Akatsuka A,Nonaka S,Shimizu H,Fukuma M,Hata J

    更新日期：2001-05-15 00:00:00

  • Retinoic acid modulates attachment of mouse fibroblasts to laminin substrates.

    abstract：:The effect of retinoic acid treatment on cell attachment to plastic substrates precoated with fibronectin, gelatin, laminin, and type IV collagen was investigated. Both retinoic acid-treated and control cells attached efficiently to fibronectin or gelatin substrates without any significant difference. In contrast, ret...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90285-0

    authors： Kato S,De Luca LM

    更新日期：1987-12-01 00:00:00

  • Simultaneous cell death and desquamation of the embryonic diffusion barrier during epidermal development.

    abstract：:The periderm is an epithelial layer covering the emerging epidermis in early embryogenesis of vertebrates. In the chicken embryo, an additional cellular layer, the subperiderm, occurs at later embryonic stages underneath the periderm. The questions arose what is the function of both epithelial layers and, as they are ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.06.009

    authors： Saathoff M,Blum B,Quast T,Kirfel G,Herzog V

    更新日期：2004-10-01 00:00:00

  • Metabolic profiling associated with autophagy of human placenta-derived mesenchymal stem cells by chemical isotope labeling LC-MS.

    abstract：:Autophagy has been reported to have a pivotal role in maintaining stemness, regulating immunomodulation and enhancing the survival of mesenchymal stem cells (MSCs). However, the effect of autophagy on MSC metabolism is largely unknown. Here, we report a workflow for examining the impact of autophagy on human placenta-...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.09.009

    authors： Sun Y,Chen D,Liu J,Xu Y,Shi X,Luo X,Pan Q,Yu J,Yang J,Cao H,Li L,Li L

    更新日期：2018-11-01 00:00:00

  • Conditional inactivation of Brca1 in the mouse ovarian surface epithelium results in an increase in preneoplastic changes.

    abstract：:Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.09.026

    authors： Clark-Knowles KV,Garson K,Jonkers J,Vanderhyden BC

    更新日期：2007-01-01 00:00:00

  • Hydrogen sulfide induces ICAM-1 expression and neutrophil adhesion to caerulein-treated pancreatic acinar cells through NF-kappaB and Src-family kinases pathway.

    abstract：:We have earlier shown that mouse pancreatic acinar cells produce hydrogen sulfide (H(2)S), which plays a key role in the pathogenesis of acute pancreatitis (AP). H(2)S-dependent induction of inflammation is mediated by the activation of transcription factor NF-kappaB. We now provide evidence that activation of Src fam...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.02.044

    authors： Tamizhselvi R,Koh YH,Sun J,Zhang H,Bhatia M

    更新日期：2010-05-15 00:00:00

  • Collective cell migration of primary zebrafish keratocytes.

    abstract：:Fish keratocytes are an established model in single cell motility but little is known about their collective migration. Initially, sheets migrate from the scale at ~145 μm/h but over the course of 24h the rate of leading edge advance decreases to ~23 μm/h. During this period, leader cells retain their ability to migra...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.06.011

    authors： Rapanan JL,Cooper KE,Leyva KJ,Hull EE

    更新日期：2014-08-01 00:00:00

  • The gene cluster for human U2 RNA is located on chromosome 17q21.

    abstract：:The gene cluster for human U2 RNA has been mapped to chromosome 17q21 by in situ hybridization and hybridization analysis of DNA from mouse/human somatic cell hybrids. ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(85)80020-3

    authors： Hammarström K,Santesson B,Westin G,Pettersson U

    更新日期：1985-08-01 00:00:00

  • Expression of dipeptidyl peptidase IV in rat tissues is mainly regulated at the mRNA levels.

    abstract：:Dipeptidyl peptidase IV (DPPIV) is a serine peptidase that cleaves N-terminal dipeptides from polypeptides when the second residue is a proline or an alanine. We have recently cloned cDNAs for rat gp110, a membrane glycoprotein with Mr of 110,000 isolated initially from rat liver. Studies reported here establish that ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90296-6

    authors： Hong WJ,Petell JK,Swank D,Sanford J,Hixson DC,Doyle D

    更新日期：1989-05-01 00:00:00